• Title/Summary/Keyword: variable region

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Comparison Measurement Error of T-score Between Lumbar and Femoral Neck According to Kind of DXA (DXA측정기 종류에 따른 요추부와 대퇴경부 골밀도 값의 측정오차 비교)

  • Han, Beom-Hee;Jung, Hong-Ryang;Lim, Cheong-Hwan;Lee, Hye-Nam;Jeong, Cheon-Soo;Lee, Sang-Ho
    • The Journal of the Korea Contents Association
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    • v.10 no.3
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    • pp.250-257
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    • 2010
  • In this study, three different devices, Norland, Osteocore and Lunar were used to compare and analyze the measurement error by each bone density measurement device by classifying the physical characteristics into age, height and weight, the subject of total 300 sampling 100 persons for each device. Categorizing Lumbar region and Femoral neck as normal (T-score$\geqq$-1.0), osteopenia (-1.0>T-score>-2.5) and osteoporosis (T-score$\leqq$-2.5), the findings were observed as follows. Norland device showed the least measurement error in age and height, while Lunar showed the least in weight among the devices. And, the result of comparing the bone density measurement error based on the lumbar region showed that all of Lunar, Norland and Osteocore have the least variation of measurement error in osteopenia and the result of comparing based on the femoral neck showed that all of Lunar, Norland and Osteocore have the least variation of measurement error in osteoporosis. For each variable, the measurement error was observed to be vary upon the device. To solve this, standardized common Phantom should be used to compare and converge the measured value of each company and cross-calibration would be necessary when replacing the software.

Investigation of PCR-RFLPs within Major Histocompatibility Complex B-G Genes Using Two Restriction Enzymes in Eight Breeds of Chinese Indigenous Chickens

  • Xu, R.F.;Li, K.;Chen, G.H.;Qiang, B.Y.Z.;Mo, D.L.;Fan, B.;Li, C.C.;Yu, M.;Zhu, M.J.;Xiong, T.A.;Liu, Bang
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.7
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    • pp.942-948
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    • 2005
  • New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a non-synonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variable-region-like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.

Preliminary Study for Tidal Flat Detection in Yeongjong-do according to Tide Level using Landsat Images (Landsat 위성을 이용한 조위에 따른 영종도 갯벌의 면적 탐지에 관한 선행 연구)

  • Lee, Seulki;Kim, Gyuyeon;Lee, Changwook
    • Korean Journal of Remote Sensing
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    • v.32 no.6
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    • pp.639-645
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    • 2016
  • Yeongjong-do is seventh largest island in the west coast of Korea which is 4.8 km away in the direction of south-west from Incheon. The mudflat area around the Yeongjong-do has variable dimension according to tide level. In addition, Yeongjong-do is important area with high environmental value as wintering sites for migratory birds. The mudflat of Yeongjong-do is also meaningful region because it is used as place of education and tourist attraction. But, there are increasing concerns about preservation of mudflat area caused by artificial development such as land reclamation project and Incheon airport construction. In this paper, mudflat area was detected using Landsat 7 ETM+ images that United States Geological Survey (USGS) is providing the data in 16 days period. The false color composite was made from band 7, 5, and 3 that could dividing boundary between water and land for the purpose of appearance of boundary line in mudflat region. This area was calculated using results of classification based on false color composite images and was digitized through repetitive algorithm during research of period. Therefore, the change of northeastern area in Yeongjong-do was detected according to tide level during 16 years from 2000 to 2015 on the basis of providing period at tide station. This paper will expect as indicator for range of area in same tide level prior to the start of the research for preservation of mudflat. It will be also scientific grounds about change of mudflat area caused by artificial development.

Genetic characterization of Shigella flexneri isolated from the diarrheic patients in Seoul region (서울지역 설사환자로 부터 분리된 Shigella flexneri의 성상과 유전적 특성)

  • Seung, Hyun-Jung;Kim, Moo-Sang;Oh, Young-Hee;Choi, Byung-Hyun;Chae, Hee-Sun;Chu, Jiaqi;Jun, Moo-Hyung
    • Korean Journal of Veterinary Research
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    • v.46 no.4
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    • pp.337-345
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    • 2006
  • The shigellae are common etiological agents of bacillary dysentery in humans and primates. During four years from 2002 to 2005, 22 strains of Shigella spp. were isolated from the diarrheic patients in Seoul region. All of them were identified as S. flexneri by biochemical tests and serotyping. The prevalence of serotypes were variable by year, but the major serotypes were 2a and 3a. In an antimicrobial susceptibility test, all of the isolates were resistant to streptomycin and tetracycline, and susceptible to amikacin, kanamycin, cefoxitin, and gentamicin. All of the isolates showed the multi-resistant patterns over 3 drugs. By analysis of the plasmid profile the isolates were classified into 7 groups (P1~P7). Serotypes 2a and 2b were distributed to P1, P2, P3, and P4. Serotype 3a was differentiated to P5 and serotype 3b, to P6 and serotype 4a, to P7. PCR results showed that all isolates were positive for two virulence genes, ipaH and ial, but none of the strains had stx gene. The set1A and set1B genes were detected from 12 isolates (54.5%) that belonged to serotype 2a and 2b. The sen gene was detected from 19 isolates (86.4%). The 22 isolates showed 12 to 17 DNA fragments in the sizes ranging from 20.5 kb to 1135 kb, resulting in 13 patterns by the PFGE with Not I digestion. The PFGE patterns of the isolates showed the close relation with the serotypes, but no relations with year of isolation and antimicrobial resistance.

Analysis and Improving ways of Factors affecting the Ill-defined Causes of Death of the Aged in Korea (노인의 불명확한 사망원인진단 관련요인 분석과 개선방안)

  • Park, Sang-Hee;Lee, Tae-Yong
    • Health Policy and Management
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    • v.21 no.2
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    • pp.329-348
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    • 2011
  • This research analyzed 168,010 cases of death of the aged over 65 from 244,867cases of death excluding 7 unknown age cases from 244,874 all age cases of death by using the death data of the National Statistical Office for 2007 to figure out factors affecting the quality of causes of death statistics of the aged and to suggest the ways of improving the quality of death statistics of the aged in korea. This research tried to derive factors affecting ill-defined cause of death category in acordance with WHO's guidelines and to find causes of lowering the accuracy of causes of death statistics of the aged. This research identified the problems of causes of death statistics of the aged by using both demographic characteristics such as sex, age, marital status, educational attainment, residential region, region size and factors of death items as independent variable to find causes of ill-defined cause of death of the aged. Logistic regression analysis was executed to calculate the hazard ratio about the ill-defined causes of death of the aged and multiple regression analysis was conducted to derive factors affecting the ill-defined cause of death by regional groups through using these independent variables such as the component ratio of over age 65, female death rates, doctors insitutions rate, medical institutions rate, attaching rates of death certificate by neighborhood. As a results of this research, R-code was the highest of ill-defined causes of death, accounting for 82.1%, and senility death(R54) of R-code was the highest, accounting for 91.2%. through subdivided order distribution of the ill-defined causes of death of the aged. As ill-defined causes of death by regional groups, attaching rates of death certificate by neighborhood was the most important factor(p<0.05) and also showed regression model's description with 83.8% ($R^2$=83.8%). Furthermore, Jeon-nam was the highest in the regional groups and these regions such as Je-ju, Jeon-buk, Chung-nam were not only attaching the death certificate by neighborhood but also were high at the rate of ill-defined causes of death. Therefore, this research found that both reconsideration about death certificate by neighborhood and education for doctors who write death certificate were needed the most.

The Relationship of Two-Point Discrimination Threshold and the Number of Fungiform Papilla According to Anatomical Location of Tongue (해부학적 위치에 따른 혀의 촉각식별능의 차이와 심상유두 개수의 관계)

  • Kim, Kyun-Yo;Hur, Yun-Kyung;Choi, Jae-Kap
    • Journal of Oral Medicine and Pain
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    • v.33 no.4
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    • pp.295-303
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    • 2008
  • The lingual branch of the trigeminal nerve transmitts general sensation from anterior two thirds of the tongue, also bearing within sheath fibers of chordal tympani branch of the facial nerve. Chorda tympani nerve carries special taste sensations from the anterior two thirds of the tongue and sub-serves the existing trigeminal pathway. Chorda tympani nerve and the lingual nerve innervate to fungiform papilla and distribution of fungiform papilla on tongue dorsum is variable according to anatomical location. The purpose of this report is to assess that the relationship of the number of fungiform papilla and the ability of two-point discrimination of tongu dorsum. Twenty-six healthy students(male:female=13:13) whose mean age was $30{\pm}3$ participated in our study. Two-point discrimination thresholds were measured to evaluate the spatial acuity of touch sensation. The measurement was carried out at the tip and posterolateral region of dorsal tongue. After two-point discrimination test, we took the pictures of their dorsal tongue dyed with methylene blue with digital camera. There were no significant differences between the number of fungiform papilla and the two-point discrimination threshold. But, we found that there were the intraregional and intersubject variations of spatial acuity of the tongue. During the test on the posterolateral region of the dorsal tongue, students appealed the difficulty of discrimination of one point and two point.

An Analysis of Operational Efficiency and Productivity for deep-sea fishing vessels in the North Pacific Ocean (북태평양 조업선박의 운영 효율성 및 생산성 분석)

  • Cho, Wooyoun;Jo, Geonsik;Yeo, Gitae
    • Journal of Korea Port Economic Association
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    • v.30 no.2
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    • pp.113-132
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    • 2014
  • With the global warming phenomenon, the deep sea water area that fishing vessels can enter and operate is ever widening. For example, the Arctic Ocean recently has overall competitive advantages due to having many deep-sea fish stocks. The North Pacific region is a strategic coastal district, the closest access point of Arctic Ocean. For Korean fishing vessels which now operate in North Pacific region, and want to entry the Arctic Ocean, the analysis of technical efficiency is needed for preparing the better industry's future. This paper aims to analyze the relative efficiency, and select the low effective deep-sea fishing vessels in the North Pacific, and to suggest their desirables strategies. As a research methodology, Data Envelopment Analysis (DEA) and Malmquist Index are applied to 16 fishing vessels for the periods(2009 to 2013). To draw out the efficiency of targeted deep-sea fishing vessels, gross tons, horsepowers, and operating days are used as input variables while total catch stands for an output variable. As a result, CCR efficiency, BCC efficiency and scalability efficiency are measured to be 0.8405, 0.9484 and 0.8858 respectively for 5 years (2009 to 2013). In conclusion, 38% of total tons, 36% of horsepowers and 29% of operating days each fishing vessel should be reduced to keep their competitive powers.

Molecular genetic analysis of phytochelatin synthase genes in Arabidopsis

  • Ha, Suk-Bong
    • Proceedings of the Botanical Society of Korea Conference
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    • 2002.04a
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    • pp.62-72
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    • 2002
  • This study has investigated the biosynthesis and function of the heavy metal binding peptides, the phytochelatins, in plants. PCs are synthesised enzymatically from glutathione by the enzyme PC synthase in the presence of heavy metal ions. Using Arabidopsis thaliana as a model organism cadmium-sensitive, phytochelatin-deficient mutants have been isolated and characterised in previous studies. The cadl mutants have wildtype levels of glutathione, are PC deficient and lack PC synthase activity. Thus, the CADl gene has been proposed to encode PC synthase. The CADl gene was isolated by a positional cloning strategy The gene was mapped and a candidate identified. Each of four cadl mutants had a single base pair change in the candidate gene and the cadmium-sensitive, cadl phenotype was complemented by the candidate gene. This demonstrated the CADl gene had been cloned. A homologous gene in the fission yeast, Schizosaccharomyces pombe was identified through database searches. A targeted-deletion mutation of this gene was constructed and the mutant, like cadl mutants of Arabidopsis, was cadmium-sensitive and PC-deficient. A comparison of the redicted amino acid sequences reveals a highly conserved N-terminal region Presumed to be the catalytic domain and a variable C-terminal region containing multiple Cys residues proposed to be involved in activation of the enzyme by metal ions. Similar genes were also identified in animal species. The Arabidopsis CADl/AtPCSl and S. pombe SpbPCS genes were expressed in E. coli and were shown to be sufficient for glutathione-dependent, heavy metal activate PC synthesis in vitro, thus demonstrating these genes encode PC synthase enzymes. Using RT-PCR, AtPCSl expression appeared to be independent of Cd exposure. However, at higher levels of Cd exposure a AtPCSl-CUS reporter gene construct appeared to be more highly expressed. Using the reporter gene construct, AtPCSl was expressed most tissues. Expression appeared to be greater in younger tissues and same higher levels of expression was observed in some regions, including carpels and the base of siliques. AtPCS2 was a functional gene encoding an active PC synthase. However, its Pattern of expression and the phenotype of a mutant (or antisense line) have not been determined. Assuming the gene is functional then it has clearly been maintained through evolution and must provide some selective advantage. This implies that, at least in some cells or tissue, it is likely to be the dominant PC synthase expressed. This remains to be determined

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An histochemical study of endocrine cells in the alimentary tract of the red-eared slider, Trachemys scripta elegans (붉은귀거북이 소화관 내분비세포에 관한 조직화학적 연구)

  • Lee, Hyeung-sik;Ku, Sae-kwang;Park, Ki-dae;Lee, Jae-hyun;Lee, Mal-soon
    • Korean Journal of Veterinary Research
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    • v.40 no.2
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    • pp.247-256
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    • 2000
  • The regional distributions and relative frequencies of endocrine cells were studied histochemically (Grimelius and Masson-Hamperl (M-H) silver methods) in the alimentary tract of the red-eared slider, Trachemys scripta elegans. Samples were taken from the esophagus, fundus, pylorus, duodenum, jejunum, ileum and large intestine. Argyrophil (Grimelius-positive) cells and argentaffin (M-H-positive) cells were found in the whole alimentary tract in this study. Spherical to spindle and/or oval to round-shaped argyrophil or argentaffin cells were located in the gastric glands of the stomach regions, in the basal portion of the epithelium of intestinal tract or the esophagus with variable frequencies. Argentaffin cells were more numerously detected in the whole alimentary tract compared with those of argyrophil cells in this study. Argyrophil cells were observed in the whole alimentary tract including the esophagus and the most predominant region was the rectum with moderate frequency. The relative frequency of these cells was rare in the esophagus, fundus, duodenum, jejunum and ileum, respectively and a few frequency in the pylorus. Argentaffin cells were also observed in the whole alimentary tract including the esophagus and the most numerously demonstrated region was the rectum with numerous frequency. They were observed with a few frequencies in the remaining regions of the alimentary tract except for the rectum, respectively. However, to know the exact type of the argyrophil cells and argentaffin cells that were observed in this study, more developmental methods such as immunohistochemistry were needed.

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Characterization of Asphalt Pavement Distress Using Korean Pavement Research Program (한국형포장설계법을 이용한 아스팔트포장의 파손특성)

  • Lee, Kwan-Ho;Lee, Kyung-Suk
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.4
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    • pp.487-493
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    • 2017
  • The main purpose of this study is to evaluate the main parameters involved in the asphalt pavement distresses, including IRI (International Rough Index), fatigue, and permanent deformation. The main parameters are the region (Seoul and Busan), traffic level, asphalt binder, maximum aggregate of surface course, thickness of the surface course and base. A total of 64 case studies were carried out under the auspices of the KPRP (Korea Pavement Research Program). From the analysis of the KPRP test results, the key factors for the asphalt pavement distress were determined. Considering the effect of one variable in the basic condition, asphalt binder was the major factor having an effect on the distresses for an AADT (Annual Average Daily Traffic) of 5000 in the Seoul area. Among the remaining factors, the results were found to be in the order of the base layer thickness (A), surface layer thickness (B), and aggregate particle size thickness (D). The same results were obtained for an AADT of 10000. In the case of Busan with an AADT of 5000, the same result was obtained as for Seoul. Among the remaining factors, the results were in the order of the base layer thickness (A), aggregate particle thickness (D), and surface layer thickness (B). Even though there was a slight difference in the effect of the traffic level and region, asphalt binder was the parameter having the greatest effect on the asphalt pavement distress. In the case where the effect of multiple parameters was analyzed, the combination of the asphalt binder and base thickness showed a relatively strong effect.