• Journal of the Korean Society of Food Culture
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• v.22 no.2
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• pp.266-273
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• 2007

This study was conducted to examine the antimicrobial activity of Perilla frutescens var. acuta leaf fractions extracted with a mixture of ethanol and water. The Ca and Mg contents of the leaf were 595.75 mg% and 467.0 mg%, respectively, and they were the highest among all of the test minerals. The extract yield increased w e content of water in e extraction solvent. Antimicrobial activity against Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa was found in the 50,70 and 95% ethanol extracts. Of the various fractions extracted from the mixture of ethanol and water, the ethyl acetate fraction showed antibacterial activity against all microorganisms tested in this experiment, and the antibacterial activity of ethyl acetate fraction from the water extract was the strongest. The phenol and flavonoid content in the ethyl acetate fraction showed no correlation with the concentration of ethanol in the extract solvent; however, their contents were higher in the 30% ethanol and water extraction which the antimicrobial activity of the extract was the strongest.

• 한국약용작물학회:학술대회논문집
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• 2011.09a
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• pp.300-301
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• 2011

• 대한한약학회:학술대회논문집
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• 2007.11a
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• pp.181-181
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• 2007

• Proceedings of the Korean Nutrition Society Conference
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• 2001.05a
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• pp.309.2-309
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• 2001

• Korean journal of applied entomology
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• v.35 no.1
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• pp.15-17
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• 1996

Acanthopsyche nigraplaga Wileman, Psychidae is reported for the first time from Korea with a brief redescription and illustration of its male genitalia. Their food plants, Gomphrena globosa L., Rhubus parvifolius L. var. triphyllus N., Perilla frutescens B. var. acuta K., Menispermum dahuricum Dc., and Chenopodium album L. are also newly reported.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.2
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• pp.192-198
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• 1999

A pot experiment was carried out to investigate the effect of boron on the growth, symptom, yield, and content of boron in Perilla(Perilla frutescens var. japonica HARA), Melon(Cucumis melo L.) and Cucumber(Cucumis sativus L.). The results obtained are as follows. Perilla. melon and cucumber uptake a lot of boron from the soil, and toxic effects of boron result in leaf tip yellowing by progressive necrosis. Yield of perilla, melon and cucumber are decreased at the $3{\sim}5mg\;kg^{-1}$, $5{\sim}10mg\;kg^{-1}$ of boron applicated in soil, respectively. Boron content in the leaf is the highest among various parts plant, and the tip and margin of the leaf contained more boron than central part of the leaf. Boron applicated was moved and accumulated to the plant and soil.

• Research in Plant Disease
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• v.22 no.3
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• pp.184-189
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• 2016

To select resistant oil seed crops against two species of root-knot nematodes, Meloidogyne incognita and M. arenaria, 10 cultivars of sesame (Sesamum indicum L.) and 10 cultivars of perilla (Perilla frutescens var. japonica) were screened in greenhouse pot test. All sesame cultivars tested were resistant to M. incognita but susceptible to M. arenaria. While, perilla was resistant to both Meloidogyne species. Therefore, perilla cultivars could be used as rotation crop in greenhouse infested with both M. incognita and M. arenaria. But, sesame cultivars only can be used as a rotation crop in greenhouse infested with M. incognita but not for M. arenaria.

• Journal of Life Science
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• v.13 no.6
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• pp.782-787
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• 2003

Perill(Perilla frutescens var. japonica $H_{ARA}$), “Ipdlkkae 1” was tested for the yield components in accordance with several different application levels, seeding methods and seeding dates. The results were summarized as follows: Application of fertilizer remarkably increased leaf number and leaf weight. Specially nitrogen showed the most significant effect. The best combination of NㆍPㆍK for cultivation of perilla was investigated ill three leveles of 6kg /10a, 9kg/10a, and 10kg/10a. The planting density in $10cm\times10cm/m^2$, and the seeding date on December 23 were found as the best results. However, $10cm\times7cm/m^2$ planting was better to get heavy leaf.Perill(Perilla frutescens var. japonica $H_{ARA}$), “Ipdlkkae 1” was tested for the yield components in accordance with several different application levels, seeding methods and seeding dates. The results were summarized as follows: Application of fertilizer remarkably increased leaf number and leaf weight. Specially nitrogen showed the most significant effect. The best combination of NㆍPㆍK for cultivation of perilla was investigated ill three leveles of 6kg /10a, 9kg/10a, and 10kg/10a. The planting density in $10cm\times10cm/m^2$, and the seeding date on December 23 were found as the best results. However, $10cm\times7cm/m^2$ planting was better to get heavy leaf.

• Korean Journal of Agricultural Science
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• v.49 no.2
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• pp.357-366
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• 2022

Biochar can be obtained by using various types of biomass under an oxygen-limited condition. Biochar can be utilized for various applications such as soil improvement, waste management, growth promotion, and adsorption. Wood vinegar is produced by the process of pyrolysis wood biomass and is used as a growth promoter, for soil improvement, and as a feed additive. When wood vinegar is treated on soil, it acts to control soil pH, improve nutrient availability, and alleviate N₂O and NH₃ volatilization. The objective of this study was to evaluate the effect of biochar and wood vinegar on the growth of perilla and soil quality. The experiment was conducted by using a Wagner pot (1·5,000 a^-1) in a glass greenhouse. The biochar was produced by pyrolysis at 450℃ for 30 minutes using rice husk and rice straw. Wood vinegar was diluted to 1 : 500 (v·v^-1) and used in this experiement. In the results of a cultivation experiment, co-application of biochar and wood vinegar enhanced the growth of perilla. In particular, rice husk biochar affected the leaves of the perilla, and rice straw biochar influenced the stems of the perilla. In addition, soil quality after treatment with biochar and wood vinegar applied together was highest compared to other units. Therefore, it is anticipated that co-application of biochar and wood vinegar will be more productive and improve soil quality compared to individual utilization of biochar and wood vinegar.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.10b
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• pp.20-20
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• 2003

Isolation and culture of leaf protoplasts from two chilli cultivars (Capsicum annuum var. accumnatum and Bird chilli) were developed to enhance selection process in the somatic hybridization programmes. In order to isolate the protoplasts from leaves of these two chilli cultivars different incubation periods (3, 5 and 10 hours) were tested with combinations of enzyme mixtures containing cellulase and macerozyme. Leaves were incubated on three enzyme mixtures (2% cellulase +0.4% macerozyme, 1% cellulase +0.2% macerozyme and 0.5% cellulase +0.1% macerozyme in 13% mannitol) at 251oC in the dark. Three hours of incubation using 2% cellulase and 0.4% macerozyme was the best for the protoplast isolation of both chilli cultivars tested. The yield was 5${\times}$108protoplasts/m1/g leaf tissue in both chilli varieties. It was found that in the mixed nurse method using Nagata and Takebe (NT) medium supplemented with 1.0mg/12,4-D, NAA and BAP with 0.5M mannitol and 1.2% Sea Plaque agarose is the best medium for protoplast culture. Protoplasts of Capsicum annum var. accumnatum were alive for 14 days forming cell walls and initiating cell division.