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http://dx.doi.org/10.7318/KJFC.2007.22.2.266

Antimicrobial Activity of Aqueous Ethanol Extracts of Perilla frutescens var. acuta Leaf  

Kim, Mi-Hyang (Department of Food Science & Nutrition, Sangju National University)
Lee, Nan-Hee (Department of Food Science & Nutrition, Catholic University of Daegu)
Lee, Myung-Hee (Department of health & Fermentation Food, Kyongbuk Collage and science)
Kwon, Dae-Jun (Department of Oriental Medicinal Resources, Asia University)
Choi, Ung-Kyu (Department of Oriental Medicinal Food and Nutrition, Asia University)
Publication Information
Journal of the Korean Society of Food Culture / v.22, no.2, 2007 , pp. 266-273 More about this Journal
Abstract
This study was conducted to examine the antimicrobial activity of Perilla frutescens var. acuta leaf fractions extracted with a mixture of ethanol and water. The Ca and Mg contents of the leaf were 595.75 mg% and 467.0 mg%, respectively, and they were the highest among all of the test minerals. The extract yield increased w e content of water in e extraction solvent. Antimicrobial activity against Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa was found in the 50,70 and 95% ethanol extracts. Of the various fractions extracted from the mixture of ethanol and water, the ethyl acetate fraction showed antibacterial activity against all microorganisms tested in this experiment, and the antibacterial activity of ethyl acetate fraction from the water extract was the strongest. The phenol and flavonoid content in the ethyl acetate fraction showed no correlation with the concentration of ethanol in the extract solvent; however, their contents were higher in the 30% ethanol and water extraction which the antimicrobial activity of the extract was the strongest.
Keywords
Perilla frutescens var. acuta; antimicrobial activity; polyphenol; flavonoids;
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Times Cited By KSCI : 9  (Citation Analysis)
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1 Hwang JH, Jang MS. 2001. Physicochemical properties of Dongchimi added with Jasoja ( Perillae semen). Korean Society of Food & Cookery Science, 17(6): 555-564   과학기술학회마을
2 Kim CK, Kim YJ, Kwon YJ. 1998. Amino Acid and Fatty Acid Compositions of Perilla semen. Korean Soci. of Food Science and Nutrition, 27(3): 381-385   과학기술학회마을
3 Lee ES. Seo BI. 2005. Growth inhibition of Perilla frutescens var. acuta extract. Korean J. Herbology. 20(2): 83-89
4 Lee OH. Lee HB, Son JY. 2004. Antimicrobial activities and nitritescavenging abiliyh of olive leaf fractions. Korean J. Soc. Food Cookery Sci. 20(2): 204-210
5 Moon GS. 1999. Medicinal herbs component and utilization. Ilwealsaegak, seoul. pp 517-518
6 Sohn J.S. and Kim M.K. 1988 Effects of hesperidin and naringin on antioxidative capacity in the rat. Korean Nutr. Soc. 31(3): 687-696
7 Swo DH and Han DS. 1998. Antitumor Effects of the Chloroform Soluble Fraction of Perilla frutescens against Human Skin Melanoma Cells. Wonkwang univ. dental medical 1.8(2): 55-66
8 Seo HH, Jeong SH and Kim DW. 2003. Research of natural dyeing development using plum - around match dyeing of plum and perilla plant-. Korea Soci. of craft, 6(2): 125-146
9 Takeda, H. Tsuii, M, Matsumiya, T. Kubo, M. 2002. Identification of Rosmarinic Acid as a Novel Antidepressive Substance in the Leaves of Perilla frutescens Britton var. acuta Kudo (Perillae Herba). Japanese Journal of Neuropsycho pharmacology-Tokyo-. 22(2): 15-22
10 Piddok, L. J. V. 1990. Techniques used for the determination of antibacterial resistance and sensitivity in bacteria. J. Appl. Bacteriol. 68(2): 307-318   DOI
11 Do JR, Heo IS, Back SY, Yoon HS, Jo JH, Kim YM, Kim KJ, Kim SK. 2006 Antihypertensive, Antimicrobial and Antifungal Activities of Buckwheat Hydrolysate. Korean Society. of food Sci. & Tech., 38(2): 268-272   과학기술학회마을
12 Yamamoto, H.; Ogawa, T. 2002. Antimicrobial activity of perilla seed polyphenols against oral pathogenic bacteria. Bioscience, Biotechnology, and Biochemistry, 66(4): 921-924   DOI   ScienceOn
13 Bauer AW, Kibby MM, Sherria JC, Turck M. 1966. Antibiotic susceptibility testing y a standardized single disk method. Am. J. Clin. Pathol. 45(4): 493-496   DOI
14 Kang YS, Lee SC, Shin HD, Shin MK, Kim JH and Song HJ. 2004. Studies on the allergy asthma effect of Folium Perillae. Korean Association of herbology,.19(3): 25-34
15 Aritomi, M. Kumori, T. Kawasaki, T. 1985. Cyanogenic glycosides in leaves of Perilla frutescens var. acuta. Phytochemistry. 24(10): 2438-2439   DOI   ScienceOn
16 Kawaguchi K., Mizuno T. Aida, K and Uchino K 1997 Hesperidine as an inhibitor of lipases from porcine pacreas and pseudomonas. Biosci. Biotchnol. Biochem. 61, 102-104   DOI   ScienceOn
17 Yonekura-Sakakibara, K. Tanaka Y. Fukuchi-Mizutani, M. Fuiiwara, H., Fukui, Y. Ashikari, T. Murakami Y. Yamaguchi, M. Kusumi, T. 2000. Molecular and Biochemical Characterization of a Novel Hydroxycinnamoyl-CoA:Anthocyanin 3-OGlucoside- 6'-O-Acyltransferase from Perilla frutescens. Plant & cell physiology. 41(4): 495-502   DOI   ScienceOn
18 Choi HS. 2004. Characteristic odor components of Perilla frutescens var. acuta kudo oil by aroma extract dilution analysis. Food Science and Biotechnology, 13(3): 279-284
19 Ha MH, Park WP, Lee SC, Choi SG, Cho SH. 2006. Antimicrobial Characteristic of Prunus mune extract. Korean Society of Food Preservation, 13(2): 198- 203   과학기술학회마을
20 Kim JG. 1997. Natural medicines dictionary. Namsandang. Seoul. p.172
21 Fujita, T. Nakayama, M. 1993. Monoterpene glucosides and other constituents from Perilla frutescens. Phytochemistry, 34(6): 1545-1548   DOI   ScienceOn
22 Oh DH, Hum SS, Park BK, Ahn C, Yu JY. 1998. Antimiceobial activityes of natural medicinal herbs on the food spoilage or foodborne disease microorganism. Korean J. Food Sci. Techol. 30: 957-963
23 Park HO, Kim CM, Woo GJ, Park SH, Lee DH, 2001. Chang EJ, Park KH. Monitoring and trends analysis of food poisning outbreaks occurrened in recent years in Korea. J. Food Hyg. safety, 16(2): 280-294   과학기술학회마을
24 Lee JJ, Kim SH, Chang BS, Lee JB, Huh CS, Kim TJ, BAEK YJ. 1999. The antimicrobials activity of medicinal plants extracts against Helicobacter Pylori. Korean J. Food Sci. Technol. 31(4): 764-770   과학기술학회마을
25 Yoshida, K. Kameda, K. Kondot, T. 1993. Diglucuronoflavones from purple leaves of Perilla ocimoides. 33(4): 917-919   DOI   ScienceOn
26 Ishikura, N.. 1981. Anthocyanins and flavones in leaves and seeds of Perilla plant. Agricultural and Biological Chemistry, 45(8): 1855-1860   DOI
27 Zheng, Z., pinkham, J. P. and Shetty, K. 1998: Identification of polymeric dye-tolerant oregano(Oreganum vulgare) colonal lines by quantifying total phenolics and peroxideasse activity. J. Agric. Food Chem. 46: 4441-4446   DOI   ScienceOn
28 Fuiiwara, H. Tanaka, Y. Fukui, Y. Ashikaki, T. Yamaguchi M., Kusumi, T. 1998. Purification and characterization of anthocyanin 3-aromatic acyltransferase from Perilla frutescens. Plant science. 137(1): 87-94   DOI   ScienceOn
29 NFRI. 1990. Manuals of Quality Characteristic Analysis for Food Quality Evalution(2). National Food Reserch Institute, Tsukuba, Japan. p 61
30 Hwang JH, Jang MS. 2003. Free Sugar, Free Amino Acid, Non- Volatile Organic Acid and Volatile Compounds of Dongchimi added with Jasoja(Perillae semen).Korean Society of Food & Cookery Science, 19(1): 1-10
31 Joe WA, Choi EY, Jeung SH, Kang BY, Son JH, An BJ, Lee CE, Choi KI, Son AR and Lee JT. 2005. Studies on the application for cosmetrics natural materials of folium perillae. Kor. J. Herbology, 20(3): 1-7
32 Moon HI, Zee OP and Shin KH. 1998. Effect of Perilla (Perilla frutescens Britton) Extract on Serum Ethanol Level and Hepatic Alcohol Dehydrogenase Activity. Korean Medical Crop Science, 6(2): 126-130
33 Choi KE, Kwag JS, Kim YO, Baek SH and Han DS 1997 Development of anthicancer agents from korean medicinal plants(part 4). Korean J. Texicol., 13(4): 311-316   과학기술학회마을
34 Povilaityte, V.; Venskutonis, P. R. 2000. Antioxidative activity of purple peril (Perilla frutescens L.), Moldavian dragonhead (Dracocephalum moldavica L.), and Roman chamomile (Anthemis nobilis L.) extracts in rapeseed oil. Journal of the American Oil Chemists' Society, 77(9): 951-956   DOI   ScienceOn
35 Ueda S., Yamashita H, Nakajima M and Kuwabara Y. Inhibition of microorganisms by spice extracts and flavoring compounds. Niippon Shokuhin Kogyo Gakkaishi, 29(3): 389-392
36 Kim YJ, Kim CK and YJ Kwon. 1997. Isolation of Antioxidative Components of Perilla semen. Korean Society. of food Sci. & Tech, 29(1): 38-48   과학기술학회마을
37 KFDA. 2003. Code DH. Code Food, Korea Food and Drug Association. Seoul, korea