• Journal of information and communication convergence engineering
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• 제17권1호
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• pp.14-20
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• 2019

Currently, microarray gene expression data take advantage of the sufficient classification of cancers, which addresses the problems relating to cancer causes and treatment regimens. However, the sample size of gene expression data is often restricted, because the price of microarray technology on studies in humans is high. We propose enhancing the gene expression classification of support vector machines with generative adversarial networks (GAN-SVMs). A GAN that generates new data from original training datasets was implemented. The GAN was used in conjunction with nonlinear SVMs that efficiently classify gene expression data. Numerical test results on 20 low-sample-size and very high-dimensional microarray gene expression datasets from the Kent Ridge Biomedical and Array Expression repositories indicate that the model is more accurate than state-of-the-art classifying models.

• BMB Reports
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• 제32권6호
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• pp.554-560
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• 1999

TTD1BI cells are non-hypersensitive to UV irradiation and perform normal genome repair of pyrimidine dimers but fail to excise 6-4 photoproducts and, concomitantly, are unable to restore RNA synthesis levels following UV irradiation. This pointed to a detect in gene-specific repair and this study was undertaken to examine repair of 6-4 photoproducts at the gene-level. The results indicated a defect in gene-specific repair of 6-4 photoproducts in active genes, although strand-specificity of 6-4 photoproduct removal was essentially similar to that of normal cells. These findings indicate that the near normal UV resistance of TTD1BI cells may be due to the inability of these cells to remove DNA lesions preferentially, as well as to the cells opting out of the cell cycle to repair damage before resuming replication.

• Bioinformatics and Biosystems
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• 제2권1호
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• pp.24-27
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• 2007

Human cell regulatory mechanism is one of suspicious problems among biologists. Here we tried to uncover the human breast cancer cell regulatory mechanism from gene expression data (Marc J. Van de vijver, et. al., 2002) using a module network algorithm which is suggested by Segal, et. al.(2003) Finally, we derived a module network which consists of 50 modules and 10 tree depths. Moreover, to validate this candidate network, we applied a GO enrichment test and known transcription factor-target relationships from Transfac(R) (V. Matys, et. al, 2006) and HPRD database (Peri, S. et al., 2003).

• 한국동물위생학회지
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• 제35권2호
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• pp.99-104
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• 2012

Otitis externa (OE) is a frequent disease in the ear canals of dogs. To identify the pathogens causing OE in dogs and to determine their antimicrobial resistances, specimens were collected from animal hospitals in Daejeon. The isolates were examined by morphological and biochemical tests, 16S rRNA analysis and antimicrobial susceptibility tests. We analyzed correlation between the isolated pathogens and external factors of dogs such as breed, age, gender, ear mite, hair in ears and experience with antibiotic therapy. Thirty three strains of bacteria were isolated from 26 of the 68 heads of dogs with OE. The most isolated bacteria were Enterococcus faecalis (E. faecalis) followed by Staphylococcus aureus (Sta. aureus), Sta. pseudointermedius, E. faecium, E. avium and Streptococcus canis (Strep. canis) in order of frequency of occurrence. Isolation frequency of Enterococcus spp. and Staphylococcus spp. were 51.5% and 45.5%, respectively. E. faecalis and E. faecium isolates showed VanB phenotype, which is resistant to vancomycin but sensitive to teicoplanin were 58% and 25%, respectively. Nine isolates among total twelve isolates of E. faecalis were isolated from the dogs treated with antibiotics. There was no methicillin-resistant Sta. aureus (MRSA), but were MR-Sta. pseudointermedius (MRSP) (57.1%) and vancomycin-resistant (VR)-Sta. pseudointermedius (14.3%) (VRSP) showing VanB phenotype. However, vanA, vanB and vanC genes were not detected in VR isolates from the dogs. Taken together, VR-Enterococcus spp. (VRE) is one of the major pathogens in domestic animals, as well as community-and hospital-acquired infection.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.118-118
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• 2005

• 한국작물학회:학술대회논문집
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• 한국작물학회 2006년도 Grop Science and Biotechnology in the 21st Century
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• pp.30.1-30.1
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• 2006

• 대한임상검사과학회지
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• 제49권4호
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• pp.401-406
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• 2017

이 연구에서 VITEK 2 시스템을 사용하여 $6{\mu}g/mL$ vancomycin이 첨가된 Enterococcosel (EV6 agar에서 배양한 327개의 검체 중 74개의 분리균(22.6%)으로 확인하였다. E. faecium은 55주(74.3%), E. gallinarum 16주(21.6%), E. casseliflavus 2주(2.7%) 및 E. avium 1주(1.4%)로 확인되었다. E. faecium의 55가지 표현형 중 vanA가 42주(76.4%), vanB가 9주(16.4%), vanC 표현형이 4주(7.3%)로 나타났다. E. gallinarum 16주와 E. casseliflavus 2주 모두 vanC 표현형을 보였으며 E. avium 1주는 vanB 표현형을 나타내었다. EV4에서만 증식된 E. faecium 1주는 VITEK2 시스템을 이용한 항균제 감수성 검사 결과 vancomycin과 teicoplanin에 모두 감수성이었고 vancomycin 내성 표현형 유전자는 PCR에 의해 검출되지 않았다. 총 327 검체를 $6{\mu}g/mL$ vancomycin (EV6 broth)을 첨가 한 Enterococoscosel broth에서 배양하여 120 균주(36.7%)가 분리되었다. 120균주에서 다중 중합 효소 연쇄반응에 의한 반코마이신 내성 유전형 실험을 실시한 결과, vanA가 51주(42.5%), vanA와 vanC가 5주(4.2%), vanC가 18주(15%), 나머지 46주(38.3%)에서는 vancomycin 내성 유전형 유전자는 검출되지 않았다.

• Parasites, Hosts and Diseases
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• 제53권6호
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• pp.713-717
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• 2015

A 23-year-old female residing in a village of Cao Bang Province, North Vietnam, visited the Hospital of Hanoi Medical University in July 2013. She felt dim eyes and a bulge-sticking pain in her left eye for some days before visiting the hospital. In the hospital, a clinical examination, an eye endoscopy, and an operation were carried out. A nematode specimen was collected from the eye of this patient. The body of this worm was thin and long and measured $22.0{\times}0.3mm$. It was morphologically suggested as an immature female worm of Angiostrongylus cantonensis. By a molecular method using 18S rRNA gene, this nematode was confirmed as A. cantonensis. This is the first molecular study for identification of A. cantonensis in Vietnam.

• 미생물학회지
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• 제48권2호
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• pp.116-124
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• 2012

2008년 3월, 서울의 상수원으로 이용되는 한강에서 분리된 Enterococcus 76주를 동정하고 이 균주들에 대한 항생제 감수성과 항생제 내성유전자 구조분석, 교차접합, PFGE 및 MLST를 실시하였다. 분리 균주 중 E. casseliflavus는 25주이었으며, E. faecalis 및 E. hirae가 각각 4주 및 1주이었다. 항생제 감수성을 조사한 결과 15주가 vancomycin에 대해 내성을 나타내었으며 E. faecium 11주와 E. casseliflavus 4주가 VRE인 것을 알 수 있었다. VRE를 대상으로 vanA 유전자 검출을 통해 6주의 E. faecium가 vanA를 가지는 VREF임을 밝혔으며, vanA가 포함된 transposon인 Tn1546 구조를 분석한 결과 Km36과 Km37은 Tn1 type, Km20과 Km38은 Tn2 type 그리고 Km 39와 Km40은 Tn3 type으로 나타났다. PFGE 결과 6주의 VREF 중 Km36과 Km37은 동일한 subtype을 나타내었으며, 나머지 4주는 각기 다른 subtype을 나타냈다. VREF 6주를 대상으로 MLST를 실시한 결과 3주는 ST78, 나머지 3주는 각각 ST18, ST192 및 ST230로 나타났다. 이들의 clonal complex는 모두 병원에서 분리되는 CC17로부터 파생된 것으로 파악되었으며 4주는 CC78에, 나머지 2주는 각각 CC18과 CC192에 포함된 것을 확인할 수 있었다.

• 한국응용곤충학회지
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• 제61권2호
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• pp.307-311
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• 2022

과실파리, 굴파리의 유충에 기생하는 Areotetes van Achterberg & Li, 2013(벌목: 고치벌과: 꽃파리고치벌아과)는 중국에서 처음으로 보고된 바 있다. 현재까지 Areotetes속에는 4종이 보고되어 있다. 금번 연구에서 Areotetes속의 1종, Areotetes carinuliferus van Achterberg & Li, 2013를 한국에서 처음으로 보고하며, 본 종의 형태 진단, 분포, 도해도를 작성하였고, 추가적으로 미토콘드리아 COI 데이터를 제공한다.