• Journal of the East Asian Society of Dietary Life
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• v.3 no.2
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• pp.155-163
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• 1993

This study was conducted to investigate the optimal condition of the extraction of red pepper oleoresin. The yield of oleoresin form red pepper was 24~28% when it was extracted by ethanol and methanol. and 8~10% by acetone, ethylene dichloride and n-hexane. But more than 60% of portion in alcohol extracts were moisture and sugars. Capsaicin and pigment were extracted more than 70% of those in red pepper when it was extracted by ethylene dichloride Those useful components were extracted most efficiently by mixed solvents of ethanol and ethylene dichloride at the ratio of 50:150. other extracting conditions were investigated in this studies, such as optimal extraction time was more than 3 hours, temperature was 55$^{\circ}C$ and particle size was finer than 250 mesh

• Parasites, Hosts and Diseases
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• v.52 no.1
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• pp.75-78
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• 2014

Sparganosis, an infection due to the plerocercoid of Spirometra erinacei, are found worldwide but the majority of cases occur in East Asia including Korea. This report is on a recurred case of sparganosis in the subcutaneous tissue of the right lower leg 1 year after a surgical removal of a worm from a similar region. At admission, ultrasonography (USG) of the lesion strongly suggested sparganosis, and a worm was successfully removed which turned out to be a sparganum with scolex. Since sparganum has a variable life span, and may develop into a life-threatening severe case, a patient once diagnosed as sparganosis should be properly followed-up for a certain period of time. Although imaging modalities were useful for the diagnosis of sparganosis as seen in this case, serological test such as ELISA should also be accompanied so as to support the preoperative diagnosis.

• Cross-Cultural Studies
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• v.33
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• pp.121-141
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• 2013

The purpose of this paper is to establish a methodology useful for identifying links between the postwar novels of Spain and Korea. We analyze the lives and actions of the characters from Nada by Carmen Laforet, and Writing on blank paper by Oh Sang Won. The characters in these novels demonstrate the challenge of adapting to the harsh reality of life because of the psychological or physical scars of war: characters such as Jungsub and Jungseo in Writing on blank paper and Juan and Andrea in Nada finally overcome their difficulties thanks to a shared sense of existential conscience and a steadfast fighting spirit; others, like Jungyun and Roman, surrender to the struggle and commit suicide, tragically succumbing to the bitter harshness of reality. Through the analysis of these novels' characters, we can empathize with the common tragedy of war-time life and death; we gain perspective on the destruction of both society and people caused by war.

• Korean Journal of Microbiology
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• v.43 no.4
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• pp.264-272
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• 2007

For the detection of human immunodeficiency virus (HIV), ultra-rapid real-time PCR methods were developed. The target DNA sequences were used 495 bp HIV-1-specific env gene (gi_1184090) and 294 bp HIV-2-specific env gene (gi_1332355). Ultra-rapid real-time PCR was peformed by $Genspector^{TM}$ (Samsung, Korea) using microchip-based, $6\;{\mu}l$ of reaction volume with extremely short running time in only 2 steps (denaturation, annealing/extension) in each cycle of PCR. Total reaction for 30 cycled ultra-rapid PCR detection including melting temperature analysis was completed in 7 min and 30 sec. The HIV-1-specific 117 bp-long or HIV-2-spe-cific 119 bp-long PCR products were successfully amplified from the minimum of template, $2.3{\times}10^3$ copies of each euv gene using 30 cycled two-steps ultra-rapid PCR. This kind of ultra-rapid real-time PCR method would be useful not only for the rapid-detection of HIV, but also rapid-detection of other pathogens.

• International Journal of Naval Architecture and Ocean Engineering
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• v.12 no.1
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• pp.657-666
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• 2020

A reliable and cost-effective technique for the development of corrosion damage model is introduced to predict nonlinear time-dependent corrosion wastage of steel structures. A detailed explanation on how to propose a generalised mathematical formulation of the corrosion model is investigated in this paper (Part I), and verification and application of the developed method are covered in the following paper (Part II) by adopting corrosion data of a ship's ballast tank structure. In this study, probabilistic approaches including statistical analysis were applied to select the best fit probability density function (PDF) for the measured corrosion data. The sub-parameters of selected PDF, e.g., the largest extreme value distribution consisting of scale, and shape parameters, can be formulated as a function of time using curve fitting method. The proposed technique to formulate the refined time-dependent corrosion wastage model (TDCWM) will be useful for engineers as it provides an easy and accurate prediction of the 1) starting time of corrosion, 2) remaining life of the structure, and 3) nonlinear corrosion damage amount over time. In addition, the obtained outcome can be utilised for the development of simplified engineering software shown in Appendix B.

• Journal of the Korean Home Economics Association
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• v.36 no.3
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• pp.173-190
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• 1998

This study reviewed both consumer movement and consumer policy of government in Japan. Their evolution since the Second World War will be reviewed and compared multiple periods, after the Second World war till the late 1960s, the period of the 1970s, the period after 1980s. In addition, firms' consumer satisfaction policies were briefly reviewed in order to fully understand consumer protection activities in japan. This study found four major characteristics of consumer protection activities led by both voluntary consumer organizations and government. First, consumer protection policy of government in Japan after the late 1960s was more active than that of consumer movement by consumer organizations. Second, major concerns for consumer protection were variant over time. The major goal in the first stage of period(during the 1960s) was to protect consumer safety from defective and dangerous goods and services, whereas the goal after the late 1970s was extended to cover various areas concerning the quality of life. Those areas refer to the quality of services, the way of sales and marketing, pollution of environment, and the quality of consuming life. Third, this study found that computer networks, aiming at collecting and analyzing the very useful to improve the consuming life of Japanese by providing sufficient consumer information to encourge rational choices of consumers. Forth, a close cooperation between the central and local administrations was crucial for the successful outcome In Japan This paper gives us useful guideline regarding how to improve consumer movement and govemments' policies for protecting consumer in Korea. In addition, other lessons on successful consumer satisfaction policies of Japanese firms may enable Korean firms to shape effective consumer policies of enhancing their competitiveness.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2005.09a
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• pp.72-77
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• 2005

Orthologs are genes having the same function across different species that specialize from a single gene in the last common ancestor of these species. Orthologous groups are useful in the genome annotation, studies on gene evolution, and comparative genomics. However, the construction of an orthologous group is difficult to automate and it takes so much time. It is also hard to guarantee the accuracy of the constructed orthologous groups. We propose a system to construct orthologous groups on many genomes automatically and rapidly. We utilize the grid computing to reduce the sequence alignment time, and we use clustering algorithm in the application of database to automate whole processes. We have generated orthologous groups for 20 complete prokaryotes genomes just in a day because of the grid computing. Furthermore, new genomes can be accommodated easily by the clustering algorithm and grid computing. We compared the generated orthologous groups with COGs (Clusters of orthologous Group of proteins) and KO (KEGG Ortholog). The comparison shows about 85 percent similarity compared with previous well-known orthologous databases.

• Journal of Dairy Science and Biotechnology
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• v.33 no.4
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• pp.253-262
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• 2015

The authentication and implications of misleading labeling in milk and dairy products is important to protect against cheating consumers from adulteration and to alert sensitive consumers to any undeclared potential allergens. This need to support milk and dairy products labeling has led to the development of specific analytical techniques for the analysis of milk and dairy products ingredients. Recently, several methods based on polymerase chain reaction (PCR), including restriction fragment length polymorphism (PCR-RFLP), multiplex PCR, species-specific PCR, and real-time PCR, have been proposed as useful means for identifying species of origin in milk and dairy products, as well as quantifying and detecting any adulteration. These methods have particular advantages owing to their high specificity and sensitivity, as well as rapid processing time. In this review, we provide an updated and extensive overview of the PCR-based methods used for milk and dairy products authentication with a particular focus on the application of PCR methods to detect adulteration.

• Journal of Microbiology and Biotechnology
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• v.24 no.6
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• pp.812-822
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• 2014

Metagenomic analysis of the human intestinal microbiota has extended our understanding of the role of these bacteria in improving human intestinal health; however, a number of reports have shown that current total fecal DNA extraction methods and 16S rRNA universal primer sets could affect the species coverage and resolution of these analyses. Here, we improved the extraction method for total DNA from human fecal samples by optimization of the lysis buffer, boiling time (10 min), and bead-beating time (0 min). In addition, we developed a new long-range 16S rRNA universal PCR primer set targeting the V6 to V9 regions with a 580 bp DNA product length. This new 16S rRNA primer set was evaluated by comparison with two previously developed 16S rRNA universal primer sets and showed high species coverage and resolution. The optimized total fecal DNA extraction method and newly designed long-range 16S rRNA universal primer set will be useful for the highly accurate metagenomic analysis of adult and infant intestinal microbiota with minimization of any bias.

• YAKHAK HOEJI
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• v.45 no.1
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• pp.71-77
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• 2001

We recently developed a high efficiency expression vectors pCK, which drives a high level of gene expression in the skeletal muscles of mice. In this study, we investigated the pharmacokinetics and biodistribution of pCK-VEGF expressing human VEGF165 after intravenous or intramuscular administration. The quantity of pCK-VEGF in the tissues of mice was measured by the PCR method which has a detection limit of approximately 1 pg of the exogenously added plasmid. In the case of intravenous administration, the half life of the pCK-VEGF plasmid in the bloodstream was 1.68 min. After intra-muscular administration, the half life of pCK-VEGF plasmid in the bloodstream was 6.78 min. At 90 min post-administration, 30% of the injected pCK-VEGF was found at the site of injection, where it persisted for up to 8 hours. Less than 1.6% of the injected pCK-VEGF plasmid DNA was detected in highly vascularized tissues such as the lung, kidney; and liver at 90 min post-administration, but the plasmid was undetectable at later time points. These results suggested that intramuscularly administrated pCK-VEGF persisted for longer periods of time in muscles than in other tissues and that direct intra-muscular injection of pCK-VEGF might be useful for local therapeutic angiogenesis.