• Title/Summary/Keyword: used media

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Effects of Low-Serum Medium and Various Culture Additives on Production of Recombinant Human Erythropoietin in CHO Cell Cultures (CHO 세포 배양을 통한 Recombinant Human Erythropoietin의 생산에서 저혈청 배지와 배양 첨가물질이 미치는 영향)

  • Lee, Kyung-Sun;Cha, Hyun-Myoung;Lim, Jin-Hyuk;Kim, Dong-Il
    • KSBB Journal
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    • v.32 no.2
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    • pp.90-95
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    • 2017
  • Mammalian cell cultures have been used extensively to produce proteins for therapeutic agent because of their ability to perform post-translational modification including glycosylation. To produce recombinant protein, many factors and parameter are considered such as media composition, host cell type, and culture process. In this study, recombinant human erythropoietin (rhEPO) producing cell line was established by using glutamine synthetase system. To reduce serum concentration in media, we compared direct adaptation with step adaptation. Cell growth was faster in step adaptation. In low-level serum media, there were insufficient glucose for cell growth. Thus, we added glucose in low-level serum media from 2 g/L to 4.5 g/L. Titer of rhEPO was higher than other conditions at 4.5 g/L of glucose. Additionally, N-methyl-D-aspartate (NMDA), 13-cis-retinal, and pluronic F-68 (PF-68) were added to enhance productivity in CHO cell cultures. In conclusion, we applied CHO cell producing rhEPO to low-level of serum in media using step-adaptation. Also, we confirmed positive effect of NMDA, 13-cis-retinal, and PF-68.

Structural Relationships Among Children's Duration of Media Use, Behavior Problems, and School Adjustment (아동의 미디어 이용시간, 문제행동, 학교생활적응 간의 구조적 관계)

  • Ahn, Subin;Kang, Boram;Lee, Kangyi
    • Korean Journal of Child Studies
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    • v.38 no.2
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    • pp.191-204
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    • 2017
  • Objective: The purpose of this study was to explore the structural relationships among duration of media use, behavior problems, and school adjustment, while accounting for gender differences. Methods: The study used 4th-wave panel data from the Korean Children and Youth Panel Study, and 2,119 first graders in elementary school were analyzed. The data were analyzed using Structural Equation Modeling. Results: The results can be summarized as follows. First, the duration of media use had a direct effect on school adjustment. Secondly, the duration of media use had an indirect effect on school adjustment through internalizing and externalizing problems. According to multigroup analysis, gender differences were found in the structural relations among variables. Conclusion: This study emphasizes the needs for media usage education to improve children's school adjustment. Furthermore, it suggests that different intervention strategies for internalizing/externalizing behavior problems are needed depending on gender.

Trust in User-Generated Information on Social Media during Crises: An Elaboration Likelihood Perspective

  • Pee, L.G.;Lee, Jung
    • Asia pacific journal of information systems
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    • v.26 no.1
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    • pp.1-21
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    • 2016
  • Social media is increasingly being used as a source of information during crises, such as natural disasters and civil unrests. However, the quality and truthfulness of user-generated information on social media have been a cause of concern. Many users find distinguishing between true and false information on social media difficult. Basing on the elaboration likelihood model and the motivation, opportunity, and ability framework, this study proposes and empirically tests a model that identifies the information processing routes through which users develop trust, as well as the factors that influence the use of these routes. The findings from a survey of Twitter users seeking information about the Fukushima Daiichi nuclear crisis indicate that individuals evaluate information quality more when the crisis information has strong personal relevance or when individuals have low anxiety about the crisis. By contrast, they rely on majority influence more when the crisis information has less personal relevance or when these individuals have high anxiety about the crisis. Prior knowledge does not have significant moderating effects on the use of information quality and majority influence in forming trust. This study extends the theorization of trust in user-generated information by focusing on the process through which users form trust. The findings also highlight the need to alleviate anxiety and manage non-victims in controlling the spread of false information on social media during crises.

A Study on the Survey Method of the Residents′ Housing Needs Using Interactive Media - Focused on the Difference Analysis of Answer Disposition by Media - (인터랙티브 미디어를 이용한 거주자 요구 조사방법에 관한 연구 1 - 매체별 응답성향 차이분석을 중심으로 -)

  • 김석태;오찬옥;박수빈;양세화
    • Korean Institute of Interior Design Journal
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    • v.13 no.5
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    • pp.114-124
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    • 2004
  • Most previous studies, in general, related to housing needs have analyzed the data from self-administered survey or interview using questionnaire or simple 2D floor plan. This study was Intended to suggest how to increase the reliability and effectiveness of such survey methods in examining the residents' housing needs. In order to accomplish the purpose, the two kinds of surveys using Web based VR media and typical questionnaire were peformed. The same questions were used for these two surveys, and they included the furniture characteristics, use of each room, adjustment of room size, preferences for interior colors, preferred furniture in living room, space layout of floor plan, housing life style, and preference for housing characteristics. The answer differences between these two were analyzed. The subjects were 402 housewives who lived in apartment houses in Haeundae, Busan. Finding were as follows: first, the media method was more likely to be effective than typical questionnaire survey in explaining housing needs for the adjustment of room size in housing unit plan. Second, the media method was more realistic and reliable than the other in comprehending the needs for the interior colors. Last, the VR media tended to be more effective than the other in understanding the space layout of floor plan. This research sheds light on the utilization of visual instrument for the analysis of needs related to space use.

Analysis on the Competition among Tourism Information Media through the Theory of the Niches (적소이론을 이용한 관광정보미디어간의 경쟁 분석)

  • Paek, Ji-Hyeon;YiKook, Jou-Yeon;Kim, Hyo-D.
    • The Journal of the Korea Contents Association
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    • v.11 no.8
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    • pp.448-458
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    • 2011
  • The availability of many smartphone applications and ubiquitous Wi-Fi technology has greatly spread the uses of smartphones. Particularly smartphones with the locative media services in tourism are booming because location-based technology such as GPS is well suit with tourism and tourists' needs. Smartphones also overcome the technological limit of traditional information media such as printed book, newspaper, etc. Based on the Niche Theory, the study examines the competitive relationships among information media that are used in tourism including smartphones, traditional paper publications, and computers. The paper also examines the niche of each medium based on the theory and finds which medium is more effective and preferred by people who utilize the services before and during their travel.

Effects of Drainage Types of Soil Media on the Plant Growing in Rooftop Planting (옥상녹화공법의 배수층 구조별 식물생육 효과)

  • Lee, Eun Yeob;Moon, Seok Ki
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.3 no.4
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    • pp.11-21
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    • 2000
  • This study was aimed to establish different drainage types of soil media on the plant growing in rooftop. For this study, experiment plots were installed on the roof of Social Science building, Chongju University, from April, 1998 to September, 1999. (1) Sand and sandy loam as base and check soils, (2) vermiculite as a inorganic soil media (3) "humus sawdust" and "burned rice hull" as organic soil media were used by various mixing ratio Zoysia japonica, was selected for the experiment. The results of this study are as follows : L5B3S2 and L5H3S2 of bad drainage character with a plastic drainage plate combination caused good growth effect on Zoysia japonica examined in 3 types of drainage layer with 5 types of soil media. From this result, it could be suggested that combined design of plastic drainage plate with 2 soil types - L5B3S2 and L5H3S2 - be desirable composition for regarding weight load and plant growth.

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CNN-based Gesture Recognition using Motion History Image

  • Koh, Youjin;Kim, Taewon;Hong, Min;Choi, Yoo-Joo
    • Journal of Internet Computing and Services
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    • v.21 no.5
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    • pp.67-73
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    • 2020
  • In this paper, we present a CNN-based gesture recognition approach which reduces the memory burden of input data. Most of the neural network-based gesture recognition methods have used a sequence of frame images as input data, which cause a memory burden problem. We use a motion history image in order to define a meaningful gesture. The motion history image is a grayscale image into which the temporal motion information is collapsed by synthesizing silhouette images of a user during the period of one meaningful gesture. In this paper, we first summarize the previous traditional approaches and neural network-based approaches for gesture recognition. Then we explain the data preprocessing procedure for making the motion history image and the neural network architecture with three convolution layers for recognizing the meaningful gestures. In the experiments, we trained five types of gestures, namely those for charging power, shooting left, shooting right, kicking left, and kicking right. The accuracy of gesture recognition was measured by adjusting the number of filters in each layer in the proposed network. We use a grayscale image with 240 × 320 resolution which defines one meaningful gesture and achieved a gesture recognition accuracy of 98.24%.

Consumer use of social media for food risk information: Survey findings in the United States and implications for the Korean context

  • Shim, Min Sun
    • Korean Journal of Health Education and Promotion
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    • v.33 no.3
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    • pp.83-93
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    • 2016
  • Objectives: This study aimed (1) to share findings from the U.S. on customer use of social media for information seeking and sharing about food recall risks, and (2) to discuss the implications of the findings for the context of food safety and risk communication in Korea. Methods: A cross-sectional survey was conducted with 1,026 social media users aged 18 years or older in the U.S., recruited from the Knowledge Network's nationally representative panel. Results: About 26 percent of respondents used social media either to seek or share food recall information in the past year, with social networking sites being the most popular tool. With respect to social media use for information seeking, being married, perceived risk of getting foodborne diseases, and trust in Internet were significant, positive predictors; being Whites and trust in health professionals were negative predictors. Social media use for information sharing was positively associated with education, being married, foodborne disease history, and perceived risk of foodborne diseases; Whites, income, and trust in health professionals were negative predictors. Conclusions: The study gives theoretical, methodological, and practical implications for the context of food safety and risks in Korea.

The Effect of Users' Motivations and Interactivity on Online Word of Mouth

  • PARK, Seolwoo
    • The Journal of Asian Finance, Economics and Business
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    • v.7 no.10
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    • pp.855-863
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    • 2020
  • The purpose of this study is to investigate the impacts of two different kinds of interactivity, such as user-to-user and user-to-media interaction, on the relationship between SNS motivation and online word of mouth (WOM). An online survey was conducted with SNS users in Korea. Using the convenience-sampling method, 300 surveys were collected and 295 were used in the actual analysis after excluding data with careless responses or missing values. Hypotheses were tested using Structure Equation Model (SEM) and path analysis by using AMOS22. The results indicate that four different SNS motivations (self-expression, relational, fun, and browsing motivation) have a partially significant positive effect on perceived user-to-user and user-to-media interaction in SNS. Although both user-to-user interactivity and user-to-media interactivity were found to have a significant effect on online word of mouth, by comparing the standardized regression coefficients in these relationships, it was found that user-to-user interactivity has a greater effect on online WOM than user-to-media interactivity. These results show that the motivated SNS users want to express their desire to communicate with other users in contrast than their desire to learn media functions when motivated SNS users reveal their personalities, knowledge, and abilities. Theoretical and managerial implications are discussed.

A Case Study on Using Interactive Media of Large Space Structure (대공간구조물의 Interactive Media 적용 사례 분석)

  • Kim, Sic;Yoon, Sung-Won
    • Journal of Korean Association for Spatial Structures
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    • v.11 no.4
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    • pp.71-78
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    • 2011
  • Interactive media are applied to inner and outer space of small public space to enhance for the interaction between the spectator and works, also widely used in the exterior wall by LED and projector for advertisement. In the other hand, case studies of interactive media for large space have not less accomplished than those of buildings and public spaces. Thus, these studies analyzed 11 large structure examples by display, content, interface, and technology device by comparison of those cases of ordinary building public space. First interactive media for large space are found to be contents(39%), and next one is play(27%), advertisement(17%) and message(17%).