• Journal of Veterinary Clinics
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• v.17 no.2
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• pp.340-345
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• 2000

The purpose of the present study was to evaluate the possibility of application of pediatric hemodialysis system to dogs weighing less than 6 kg. Six healthy dogs (B .W, 3-6 kg) were used_ Experimental end-stage renal failure was induced by bilateral nephrectomy or bilateral ligation of ure-ters. Hemodialysis was performed when blood urea nitrogen (BUN) value increased over 90 mg/dl and every other day thereafter. Daily investigated parameters included clinical clinical signs such as vomiting, fecal appearance and activity and laboratory data such as PCV, WBC, RBC, BUN, creatinine, Ca, P, $Na^+ Cl^-and K^+$During hemodialysis treatment, BUN and creatinine values were measured hourly. Severe vomiting and inappetence were shown 2 days after infliction of kidney disorder and melena and mucous faces were observed 3 days. The signs were not corrected by hemodialysis treatment. Avel- age hemodialysis treatment time was $5.5 {\times} 0.7$,/TEX> hours until BUN value decreased to normal range. Abnor- mal hematological and electrolytes values were reduced within normal levels after hemodialysis treatments. The complications oft hemodialysis included vomiting, nausea, obstruction of intravascular catheter, tremor, seizure, temporary visual loss and continued decrease in PCV It is suggested that pediatric hemedialysis system can be applied to acute renal failure and acute toxicity. Further works on improvements in maintaining patency of catheter and in managing the complications of hemo- dialysis should be conducted.

• Journal of Environmental Health Sciences
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• v.22 no.4
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• pp.91-101
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• 1996

Exposure indices are important tools which enable scientists to reliably predict and detect exposures to xenobiotics and resultant cell injury. Since the de novo synthesis of stress proteins can be detected early after exposure to some agents, analysis of toxicant-induced changes in gene expression, i.e. alterations in patterns of protein synthesis, may be useful to develop as biomarkers of exposure and toxicity. The acute and chronic effects of cadmium(Cd, $CdCl_2$ 20 mg/kg) on Wistar male rats were evaluated concerning cadmium contents, tissues enzyme activity, HSP expression. The results of the study were as follows: 1. Less cadmium was absorbed through the digestive tracts, but the ratio of contents in renal to hepatic cadmium was higher at 8 weeks after treatment. 2. ALT(alanine aminotransferase), AST(aspartate aminotransferase), glucose, BUN(blood urea nitrogen), creatinine, the key indices of the clinical changes in hepatic and renal function were significantly changed by the cadmium treatment after 1 week in liver, after 4 weeks in kidney. 3. Enhanced synthesis of 70 KDa relative molecular mass proteins were detected in 2 hours after cadmium exposure, with maximum activity occurring at 8~48 hours. Induction of $HSP_{70}$ was evident at proximal tubules and glomeruli in kidney. Testicular cells produced enough HSP to be detected normally. From the above results, it could be concluded that $HSP_{70}$ induction by the cadmium treatment was a rapid reaction to indicate the exposure of xenobiotics.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.1
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• pp.41-48
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• 1999

This experiment was carried out to determine the effect of the silage additives on improvement of quality of fresh rice straw silage using round bale at the forage experimental field, grassland and forage crops division, National Livestock Research Institute, RDA, Suwon from 1997 to 1998. The experiment was arranged in a randomized block design with three replication. The treatments used in this study were consisted of different additives(control, formic acid, molasses, molasses+urea and inoculant). The rice straw silage with molasses+urea treatment resulted in high crude protein content and in vitro dry matter digestibility were increased with molasses of inoculant treatments compare with the control. The mean dry matter of formic acid treatment material was higher than with control but there was no significant difference in dry matter content among the additives treatments. The pH of molasses treatments significantly increased the proportion of lactic acid(P<0.05) and decreased the proportion of butyric acid. The total organic acid content of all treatments had low around 2%. Ammonia-N of molasses+urea treatment was significantly(P<0.05) higher than that of others, but formic acid or inoculant treatments was lower below 10% per total nitrogen. Over a 7d feeding period, the dry matter intake per cattle on the inoculant treatment was higher that on both the untreated round bale silage of fresh rice straw and rice straw hay. Producing cost per kilogram of round bale silage of fresh rice straw was decreased according to the increasement of harvesting area. It is suggested that application of round bale silage system to fresh rice straw with molasses or inoculant was the best treatment for improving preservation as silage, and that animal intake was enhanced by addition of inoculant to fresh rice straw.

• Journal of the Korean Wood Science and Technology
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• v.46 no.2
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• pp.155-165
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• 2018

The objective of the research was to evaluate the effect of particle pre-treatment on physical, mechanical, and durability of jatropha fruit hulls (JFH) particleboard. The pre-treatments included were immersing in cold water, hot water, and acetic acid solution. After each treatment, the particles were dried up to 3% moisture content. Urea-formaldehyde (UF) resin was used to fabricate particleboards with board size, thickness and density target of 25 cm by 25 cm, 0.80 cm, and $0.70g/cm^3$, respectively. Board pressed at $130^{\circ}C$ for 10 minutes, and $25kg/cm^2$ pressure. The evaluation of particleboard followed the JIS A 5908-2003. Whilist their resistance to subterranean termite test (mass loss, mortality, antifeedant value and feeding rate) refers to the Indonesian standard (SNI 01.7207-2006). The physical and mechanical properties of particleboards showed that all pre-treatments decreased the pH of particles. Overall, all particle immersing treatments resulted of better physical and mechanical properties of particleboard than those of untreated ones. The acetic acid treatment resulted the best physical and mechanical properties of particleboard. Based on the mass loss of JFH particleboard, hot water and acetic acid treated particleboards were classified into weak resistance to subterranean attack. The other two treatments were classified into very weak resistance. Hot water treated particleboard provided the highest mortality and antifeedant as much as 87.40% and 34.20%, respectively. Based on antifeedant classification, hot water treated particleboards were classified into moderately strong resistance, while other treatments were categorized into weak resistance. The lowest feeding rate value ($45.30{\mu}g/termite/day$) was attained by hot water treatment.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.8
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• pp.1130-1134
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• 2005

An experiment was performed using 20 calves of about one-month old to investigate the effect of chelation therapy with calcium disodium ethylenediaminetetraacetate ($CaNa_2$EDTA) alone or along with antioxidant $\alpha$-tocopherol in lead loaded calves on blood trace minerals, erythrocytic sulfahydryl groups and some haematobiochemical parameters. Fifteen calves were given lead orally at a daily dose of 7.5 mg of 99% pure lead acetate/kg body weight for 28 days. Then the lead was withdrawn on day 28 and the calves were randomly divided into three groups. Each group of five animals was either treated with $CaNa_2$EDTA alone at the dose rate of 110 mg/kg body weight in two divided doses for 4 days or along with $\alpha$-tocopherol at the dose rate of 100 mg/kg body weight orally daily for 7 days, keeping the remaining five calves as lead-exposed untreated controls. Blood samples were collected at the end of the lead exposure (day 0) and thereafter on day 2, 4, 7 and 10 from the start of the chelation treatment. The treatment with EDTA alone led to slow but non-significant improvement in blood copper level, but incorporation of antioxidant $\alpha$-tocopherol in chelation therapy resulted in its significant decline, as recorded on day 7-post treatment. Withdrawal of lead or treatment with $CaNa_2$EDTA alone or along with $\alpha$-tocopherol enhanced the erythrocytic thiol contents and the levels of T-SH and P-SH became statistically (p<0.05) comparable to those of lead-exposed controls by day 7 and 4, respectively. There was no significant (p>0.05) change in serum urea, creatinine, total protein and albumin levels between the treatment groups. It is concluded from the present investigation that treatment with $CaNa_2$EDTA at the present dose rate is safe to be used for chelation in lead loaded calves.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.8
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• pp.1230-1237
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• 2018

Objective: The present study was undertaken to determine an optimal balance between the amount of physically effective neutral detergent fiber (peNDF) to metabolizable glucose (MG) on rumen fermentation, blood metabolites and growth performance of 8 to 10-month-old heifers. Methods: A total of 15 healthy Holstein heifers weighing an average of 256 kg (8 month of age) were randomly assigned to three groups of five. Treatment diets consisted of the following three $peNDF_{8.0}/MG$ levels: 1.46 (Treatment A), 1.74 (Treatment B), and 2.08 (Treatment C). Results: The results showed that the ratio of $peNDF_{8.0}/MG$ affected rumen fermentation, blood metabolites and growth performance of heifers. The average daily gain of heifers tended to decrease as the ratio of $peNDF_{8.0}/MG$ increased (p = 0.07). The concentrations of blood urea nitrogen, triglyceride, and cholesterol increased significantly (p<0.05), while the high-density lipoprotein concentration decreased (p<0.05). After feeding 2 h and 4 h, insulin concentration in Treatment A was greater than Treatment C (p<0.05). Propionate concentration had decreasing trend (p = 0.07); acetate to propionate ratio and non-glucogenic to glucogenic volatile fatty acid (NGR) increased significantly (p<0.05). In addition, the digestibility of dry matter, crude protein, neutral detergent fiber, and acid detergent fiber decreased significantly (p<0.05). Conclusion: The present investigation indicated that dietary $peNDF_{8.0}/MG$ ratio can affect the growth and development, blood metabolites, rumen fermentation and apparent digestibility of heifers, and the optimal dietary $peNDF_{8.0}/MG$ ratio for 8 to 10-month-old heifers in the present study was 1.46.

• Animal Bioscience
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• v.35 no.10
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• pp.1556-1565
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• 2022

Objective: Tan lambs (n = 36, 3 mo old, 19.1±0.53 kg) were used to assess effects of dietary guanidinoacetic acid (GAA) and rumen-protected methionine (RPM) on growth performance, carcass traits, meat quality, and serum parameters. Methods: Lambs were randomly assigned to three treatment groups, with 6 pens per group and 2 lambs per pen. Dietary treatments were: basal diet alone (I); basal diet supplemented with 0.08% GAA+0.06% RPM (II); and basal diet supplemented with 0.08% GAA+0.08% RPM (III). Diets were provided three times a day for 90 d. Intake per pen was recorded daily and individual lamb body weight (BW) was measured monthly. Carcass traits were measured after slaughter and meat quality at the end of the experiment, blood samples were taken on a subgroup of lambs for analysis of indicators mostly related to protein metabolism. Results: Final BW and average daily gain for the first and second month, and for the entire experiment were greater in Treatment II compared to Treatment I (p<0.05), whereas feed to gain ratio was lower (p<0.05). Treatment II had the optimal dressing percentage and net meat weight proportion, as well as crude protein and intramuscular fat concentrations in muscles. Treatment II improved meat quality, as indicated by the greater water holding capacity, pH after 45 min and 48 h, and lower shear force and cooking loss. Dietary supplementation of GAA and RPM also increased the meat color a^* and b^* values at 24 h. Finally, Treatment II increased total protein, and serum concentrations of albumin and creatinine, but decreased serum urea nitrogen concentrations, indicating improved protein efficiency. Conclusion: In this study, 0.08% GAA+0.06% RPM supplementation improved growth performance and meat quality of Tan lambs.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.10
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• pp.1518-1523
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• 2003

This experiment was conducted to investigate the effect of various feeding regimens on growth performance, nutrient digestibilities, nitrogen retention, blood urea nitrogen (BUN) concentration and feed cost in young pigs weaned at 21 days of age. One hundred twenty crossbred pigs (Landrace${\times}$Large White${\times}$Duroc, average 6.8 kg BW), weaned at 21 days of age, were allotted to 5 treatments in a 5 replicates by a randomized completely block (RCB) design. Treatments were as follow: 1) 1P (1-4 weeks: CP 23% and lysine 1.60%), 2) 2P-I (1 week: CP 23% and lysine 1.60%, 2-4 weeks: CP 21% and lysine 1.45%), 3) 2P-II (1-2 weeks: CP 23% and lysine 1.60%, 3-4 weeks: CP 21% and lysine 1.45%), 4) 2P-III (1-3 weeks: CP 23% and lysine 1.60%, 4 week: CP 21% and lysine 1.45%), 5) 3P (1 week: CP 23% and lysine 1.60%, 2-3 weeks: CP 21% and lysine 1.45%, 4 week: CP 19% and lysine 1.30%). Three different diets were formulated and supplied according to phase feeding programs. Diet 1 contains 23% crude protein and 1.60% lysine, diet 2 contains 21% crude protein and 1.45% lysine and diet 3 contains 19% crude protein and 1.30 lysine, respectively. Although there was no significant difference in growth performances, there was a beneficial effect of 3 phase feeding. The ADG was higher in 3P treatment than other treatments and it was observed clearly in late period (3-4 weeks) than in early period. Also, with increase in age, growth rate of pigs in 3P treatment was higher than that in 1P treatment approximately 37% (p=0.1379). There were no significant differences among all treatments in nutrient digestibility. The concentration of BUN was higher in pigs were fed diet containing 21% crude protein and 1.45% lysine (eg, 2P-1 and 3P) than those supplied diet containing high nutrient value at 2 week. The lowest feed cost/kg weight gain of pigs showed in 3P among treatments (p<0.05) whereas, high feed cost/kg weight gain of pigs was calculated in 1P and 2P-II treatments compared with 2P-I and 2 P-II (p<0.05), because of high milk products were used in those diet.

• Korean Journal of Soil Science and Fertilizer
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• v.27 no.1
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• pp.40-47
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• 1994

The objective of this research was to examine the effect of ammonium thiosulfate(ATS) on urease activity and on biological and chemical properties of flooded paddy soil especially having high organic matter content by comparing with the effect of sodium thiosulfate(STS). The results obtained are summarized as follows. 1. The hydrolysis of urea was inhibited at 3 and 5 days after treatment of thiosulfate(ATS and STS) +glucose and thiosulfate only, respectively. The inhibitory effect of ATS on urea hydrolysis was slightly lower than that of STS in glusoce-added soils, but when the glucose was not added, the effects of ATS and STS were not different significantly. 2. The soil pH and Eh was lowered by 0.3~0.5 units and 30~120 mV, respectively, when incubated flooded soil with ATS and glucose at $25^{\circ}C$. 3. Soil respiration rate in/flooded soil was increased by 10~70% with the treatment of ATS during the 20 day experimental period. 4. The contents of acetic and butyric acid in thiosulfate treatment soil was below $10{\mu}g/g$, which was lower than that($220.3{\mu}g/g$) of critical growth inhibition of rice.

• Journal of Veterinary Clinics
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• v.24 no.2
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• pp.94-98
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• 2007

This study was to investigate the effects of ascorbic acid and alpha-tocopherol on the attenuation of renal ischemia-reperfusion (IR) injury in pigs. Ten pigs were subjected to 60 minutes of warm unilateral renal ischemia followed by removal of contralateral kidney and then divided into two groups. Treatment group was performed ascorbic acid and alpha-tocopherol pretreatment 2 days before operation and ascorbic acid with heparin-saline solution irrigation-aspiration. Otherwise, control group used only irrigation-aspiration of heparin-saline solution. Blood samples were collected from these pigs for measurement of serum blood urea nitrogen (BUN) and creatinine values, antioxidant superoxide dismutase (SOD) at pre, day 1, day 3, day 7 and day 14. The kidneys were taken for histopathologic evaluation after euthanasia on postoperative day 14. The levels of BUN were significantly increased in the control group on day 1, day 3 and day 7 (P<0.05). And the level of creatinine was significantly increased in the control group on day 3 (p<0.05). Activity of antioxidant enzymes in plasma revealed significant difference (p<0.05) between control and treatment group at day 14. In histopathologic findings, treatment group was showed less damage than that of control group on the basis of renal tubular damage. It was concluded that ascorbic acid and alpha-tocopherol attenuated renal I/R injury in the pigs.