• 한국환경성돌연변이발암원학회지
• /
• 제21권2호
• /
• pp.89-94
• /
• 2001

Recently, single cell gel electrophoresis, also known as comet assay, is widely used for the detection and measurement of DNA strand breaks in vitro and in vivo in many toxicological fields such as radiation exposure, human monitoring and toxicity evaluation. As well defined, comet assay is a sensitive, rapid and visual method for the detection of DNA strand breaks in individual cells. Briefly, a small number of damaged cells suspended in a thin agarose gel on a microscope slide were lysed, unwinded, electrophoresed, and stained with a fluorescent DNA binding dye. The electric current pulled the charged DNA from the nucleus such that relaxed and broken DNA fragments migrated further. The resulting images which were subsequently named for their appearance as comets, were measured to determine the extent of DNA damages. However, some variations could be occurred in procedures, laboratories's conditions and kind of cells used. Hence, to overcome and to harmonize these matters in comet assay, International Workshop on Genotoxicity Test Procedure (IWGTP) was held with several topics including comet assay at Washington D.C. on March, 1999. In spite of some consensus in procedures and conditions in IWGTP, there are some problems still remained to be solved. In this respect, we attempted to set the practical optimal conditions in the experimental procedures such as lysis, unwinding, electrophoresis and neutralization conditions and so on. First of all, we determined optimal lysis and unwinding time by using 150 $\mu$M methyl methanesulfonate (MMS) which is usually used concentration. And then, we determined optimal positive control concentrations of benzo(a)pyrene (BaP) and MMS in the presence and absence of S9 metabolic activation system, respectively.

• 한국생산제조학회지
• /
• 제9권5호
• /
• pp.65-72
• /
• 2000

This paper presents the study of the tension control in a web transfer system. In this study the sliding mode controller is applied to a time-varying nonlinear mathematical model. The model was derived to consider the effects of changing the roll radius in tension variation during winding and unwinding. The uncertainty in modeling may be due to unmodelled dynamics, on variations in system model. Designed sliding mode controller made the system error always staying in the suggested surface from the beginning. Through this, system is maintained to be robust against a disturbance and uncertainty. To verify the designed controller has a good performance, various inputs such as desired velocity, step input, and trapezoidal input are applied. When the sliding mode controller was used, the system(the tension control) performance was improved comparing to the PID controller. The robustness of the controller with respect to an estimation error was verified through simulations.

• BMB Reports
• /
• 제28권1호
• /
• pp.1-5
• /
• 1995

The effect of 6-sulfooxymethyl benzo(a)pyrene (SMBP) on conformational changes of calf thymus DNA was investigated. As SMBP is a strong electrophile, the covalent binding of SMBP to DNA should distort three dimensional conformation of DNA at the binding sites. A formaldehyde-unwinding methods were used to determine the rate of DNA denaturation. The increase in absorbance at 251nm was detected by addition of formaldehyde following treatment with SMBP. SMBP changed supercoiled DNA to relaxed and linear DNA as determined by electrophoresis, which was similar to the change in DNA due to in vitro treatment with benzo(a) pyrene diol epoxide. Treatment with SMBP completely denatured DNA under alkaline conditions. However, DNA was nicked or partially denatured under neutral condition. The absorption band of DNA was increased by the treatment with SMBP in V79 cells, which may be explained by the formation of stabilized SMBP-DNA adduct.

• 한국정밀공학회:학술대회논문집
• /
• 한국정밀공학회 1994년도 추계학술대회 논문집
• /
• pp.584-589
• /
• 1994

A time-varying nonlinear mathematical model was derived to consider the effect of change in roll radius on tension varation during winding and unwinding. A variable-gain PID controller was designed for tension control at start and stop in a multi-span continuous process system. The controller gains are updated at every control loop as roll radii continuously change. Computer simulation was carried out by using the mathematical model and the controller developed for a typical operating condition including acceleration and deceleration. When the variable-gain PID controller was used, the tension control performance was improved compared with that of existing control method during start-up and stop.

• 한국정밀공학회:학술대회논문집
• /
• 한국정밀공학회 2006년도 춘계학술대회 논문집
• /
• pp.281-282
• /
• 2006

Tension control performance is very important in high-speed printing machine. One of the major factors that effect to tension control performance is the process of roll changing. Even if the turret arm moves during roll changing process and the span length of the unwinding system varies, it is customary to neglect it in motion and tension control and to consider it as a disturbance. In this paper, its effect is modeled nonlinearly and compared with linear model, and an effect of an infeeder dancer is analyzed under the condition with no unwinder dancer. We verify the performance of the proposed method via simulation in the high-speed printing machine.

• Molecules and Cells
• /
• 제26권3호
• /
• pp.308-313
• /
• 2008

In animals, microRNAs (miRNAs) and small interfering RNAs (siRNAs) repress expression of protein coding genes by assembling distinct RNA-induced silencing complexes (RISCs). It has previously been shown that passenger-strand cleavage is the predominant mechanism when siRNA duplexes are loaded into Argonaute2 (Ago2)-containing RISC, while an unwinding bypass mechanism is favored for miRNA duplexes with mismatches. Here I present experimental data indicating that some mammalian miRNAs are assembled into Ago2-containing RISC by cleaving their corresponding miRNA star strands. This phenomenon may depend on the secondary structure near the scissile phosphate of the miRNA duplex. In addition, I show that ATP is not required for star-strand cleavage in this process. Taken together, the data here provide insight into the miRNA-loading mechanisms in mammals.

• 한국염색가공학회지
• /
• 제20권1호
• /
• pp.28-35
• /
• 2008

Texturing is the process of including a characteristic of a natural fiber in a synthetic fiber. The most common method of it the false twist texturing. Nylon textured yarn is primarily manufactured by the disk type. The major process parameters or the disk type false twist machine ratio, disk/yarn, and heater temperature. This study therefore investigated the effects of false twist texturing, especially speed and draw ratio, on the physical properties of nylon textured yarn. The increase of speed was proportional to the increase of unwinding tension, which could reduce the production efficiency by elevating the tension affecting to fiber during the process. In addition, the increase of speed was inversely proportional to the increase of crimp rigidity of nylon textured yarn. Draw ratio was proportionally increased with the increase of tenacity and the reductions of fineness and elongation, showing the influence or draw ratio to the ultimate physical properties of textured yarn.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제9권1호
• /
• pp.23-34
• /
• 2004

Escherichia coli transcription termination factor Rho catalyzes the unwinding of RNA/DNA duplex in reactions that are coupled to ATP binding and hydrolysis. Fluorescence stopped-flow methods using ATP and the fluorescent 2'(3')-O-( N-methylanthraniloyl) derivatives (mant-derivatives) of ATP and ADP were used to probe the kinetics of nucleotide binding to and dissociation from the Rho-RNA complex. Presteady state nucleotide binding kinetics provides evidence for the presence of negative cooperativity in nucleotide binding among the multiple nucleotide binding sites on Rho hexamer. The binding of the first nucleotide to the Rho-RNA complex occurs at a bimolecular rate of 3.6${\times}$10$\^$6/ M$\^$-1/ sec$\^$-1/ whereas the second nucleotide binds at a slower rate of 4.7${\times}$10$\^$5/ M$\^$-1/ sec$\^$-1/ at 18$^{\circ}C$, RNA complexed with Rho affects the kinetics of nucleotide interaction with the active sites through conformational changes to the Rho hexamer, allowing the incoming nucleotide to be more accessible to the sites. Adenine nucleotide binding and dissociation is more favorable when RNA is bound to Rho, whereas ATP binding and dissociation step in the absence of RNA occurs significantly slower, at a rate ∼70- and ∼40-fold slower than those observed with the Rho-RNA complex, respectively.

• 한국생산제조학회지
• /
• 제22권4호
• /
• pp.755-760
• /
• 2013

The dynamics of the winder roller of a roll-to-roll printing system for printed electronics is a time-varying system because of the variation of the winder roller radius owing to rewinding or unwinding of the web. Therefore, an adaptive control method considering the time-variant characteristics is required for precise tension control. In this study, the variable PID gain method is applied to the actual roll-to-roll system and verified by experiments for unwinder tension control. The required value of the winder roller radius for the application of the variable PID gain is estimated from the measurement of the winder tension and winder motor torque. The simulation results as well as experimental results show that the fixed PID gain control cannot stabilize the tension of the winder roller with varying winder roller radius. On the other hand, the variable PID gain method can control the tension of the winder roller regardless of the winder roller radius.

• Journal of Microbiology
• /
• 제44권1호
• /
• pp.11-22
• /
• 2006

Escherichia coli protein Rho is required for the factor-dependent transcription termination by an RNA polymerase and is essential for the viability of the cell. It is a homohexameric protein that recognizes and binds preferably to C-rich sites in the transcribed RNA. Once bound to RNA, it utilizes RNA-dependent ATPase activity and subsequently ATPase-dependent helicase activity to unwind RNA-DNA hybrids and release RNA from a transcribing elongation complex. Studies over the past few decades have highlighted Rho as a molecule and have revealed much of its mechanistic properties. The recently solved crystal structure could explain many of its physiological functions in terms of its structure. Despite all these efforts, many of the fundamental questions pertaining to Rho recognition sites, differential ATPase activity in response to different RNAs, translocation of Rho along the nascent transcript, interactions with elongation complex and finally unwinding and release of RNA remain obscure. In the present review we have attempted to summarize 'the knowns' and 'the unknowns' of the Rho protein revealed by the recent developments in this field. An attempt has also been made to understand the physiology of Rho in the light of its phylogeny.