• Journal of Environmental Science International
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• v.18 no.4
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• pp.463-471
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• 2009

A study on vegetation in the Mangyeong River and Dongjin River basins and the surrounding regions of the Saemangeum Reclaimed Land was conducted in a series of efforts to determine the expected ecological changes in the salt marshes, to restore their vegetation, to explore the restoring force of halophyte, to examine the community mechanism and, ultimately, to rehabilitate marshy land vegetation along the lakeside, coastal dune and salt marshes of the Saemangeum Project Area. The findings of the study may be summed up as follows: Five species such as Suaeda japonica, Salicornia herbacea, Atriplex gmelini, Aster tripolium and Suaeda asparagoides that are mostly distributed in the estuary of the Saemangeum Reclaimed Land were analyzed to examine the mechanism of halophyte to maintain their community. To find out the strategies of plants for survival and the cause of forming community structure, a research was made as for appearance ratio of biomass, root lengths and germination. With regard to biomasses of halophyte, the biomass of Suaeda japonica increases rapidly, while Salicornia herbacea adopts a strategy of unstable growth pattern by which the biomass increases slowly in parallel with its slow speed of growth since initial appearance of young sapling. In contrast, Suaeda asparagoides, Atriplex gmelini and Aster tripolium choose to adapt themselves to environment promptly by being transformed into life form of annual or biennial plant, a pattern that is presumed to be favorable and stable for survival in the later stage of growth. In short, there is a sharp distinction among the 5 species: i.e. Suaeda japonica that begins to grow fast in the length of surface and underground section but slows down from the mid-stage on; Salicornia herbacea that grows slowly in the beginning step but starts to step up from the middle onward; Atriplex gmelini and Aster tripolium growing slowly in the initial stage but fast later on; and Suaeda asparagoides that turns from the constant growth in the beginning to rapid growth in the later period. The outcomes of the analysis into status of growth and influencing factors of Suaeda japonica in the sowing field that is most widely prevalent in the Saemangeum Reclaimed Land as a sort of ecological pioneer in the salt marshes showed that the average size of grass lands, density and number of individuals increase in the natural sowing field as well as in the plowing field regardless of their physical as well as physico-chemical features of the soil as the season progresses from June to October of a year.

• Membrane and Water Treatment
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• v.11 no.1
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• pp.79-85
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• 2020

A single-stage deammonification with a sequencing batch reactor (SBR) that simultaneous nitritation, anaerobic ammonia oxidation (anammox), and denitrification (SNAD) occur in one reactor has been widely applied for sidestream of wastewater treatment plant. For the stable and well-balanced SNAD, a feeding strategy of influent wastewater is one of the most important operating factors in the single-stage deammonification SBR. In this study, single-stage deammonification SBR (working volume 30L) was operated to treat a high-strength ammonium wastewater (1200 mg NH₄⁺-N/L) with different feeding strategies (single feeding and nine-step feeding) under the condition without COD. Each cycle of the step feeding involved 6 sub-cycles consisted of aerobic and anoxic periods for partial nitritation (PN) and anammox, respectively. Contrary to unstable performance in the single feeding, the step feeding showed better deammonification performance (0.565 kg-N/m³/day). Under the condition with COD, however, the nitrogen removal rate (NRR) decreased to 0.403 kg-N/m³/day when the Nine-step feeding strategies had an additional denitrification period before sub-cycles for PN and anammox. The NRR was recovered to 0.518 kg-N/m³/day by introducing an enhanced multiple-step feeding strategy. The strategy had 50 cycles consisted of feed, denitrification, PN, and anammox, instead of repeated sub-cycles for PN and anammox. The multiple-step feeding strategy without sub-cycle showed the most stable and excellent deammonification performance: high nitrogen removal efficiency (98.6%), COD removal rate (0.131 kg-COD/m³/day), and COD removal efficiency (78.8%). This seemed to be caused by that the elimination of the sub-cycles might reduce COD oxidation during aerobic condition but increase the COD utilization for denitrification period. In addition, among various sensor values, the ORP pattern appeared to be applicable to monitor and control each reaction step for deammonification in the multiple-step feeding strategy without sub-cycle. Further study to optimize the number of multiple-step feeding is still needed but these results show that the multiple-step feeding strategy can contribute to a well-balanced SNAD for deammonification when treating high-strength ammonium wastewater with COD in the single-stage deammonification SBR.

• Microbiology and Biotechnology Letters
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• v.18 no.1
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• pp.81-88
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• 1990

For the selection of powerful antagonistic bacterium for biological control of soilborne Fusarium solani causing root rot of many important crops, the best YPL-1 strain was selected among 300 strains of bacteria isolated from rhizosphere in ginseng root rot-suppressive soil. The strain was identified to be a species to Pseudomonas stutzeri. With in vitro fungal inhibition tests, antagonistic substance of P. stutzeri YPL-1 against F. solani was presumed to be heat unstable, macromolecular substances such as protein. Also, it was shown that antifungal activity of P. stutzeri YPL-1 increased in proportion to its chitinase production. P. stutzeri YPL-M122 (chi-, lam -) which was deprived of the productivity of chitinase and laminarinase by NTG mutagenesis had lost antifungal activity, completely. And P. stutzeri YPL-MI53 (chi-) had only 4.1% of its antifungal activity. P. stutzeri YPL-1 was not able to produce any extracellular siderophore in iron-deficent minimal medium. It is confident that the antifungal mechanism of P. stutzeri YPL-1 for biocontrol of F. solani depends on lysis rather than antibiosis :the mechanism of lysis appears to involve enzymatic degradation of the cell will components of F. solani by hydrolytic enzymes of more chitinase and less laminarinase.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.2
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• pp.132-138
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• 2006

Direct thrombin inhibitor, which is effective to prevent or cure the thrombosis, has been investigated in worldwide. In this study, we tried to screen antithrombosis agent from edible or medicinal plant. A strong antithrombin activity was identified from methanol or $95\%$ ethanol extract of buckwheat seeds. The solvent fractionation of buckwheat extracts using hexane, ethylacetate, butanol revealed that the butanol fraction has a prominent antithrombin activity. Thrombin time (blood-clot formation time) exceeded to over $2,000\%$ by addition of the butanol fraction at concentration of $312.5{\mu}g/mL$, whereas thrombin time extended to $336\%$ by addition of aspirin at concentration of $1,500{\mu}g/mL$. The butanol fraction showed anthrone-positive and ninhydrine-negative reaction. The active components were heat-liable, acid-unstable non-proteinous macromolecules (>30 KD). In vivo analysis using ICR male mouse showed that the buckwheat extract was superior than the aspirin in pulmonary thrombosis, KCN-induced coma and death. Our results suggest that the buckwheat is a potential as an antithrombosis agent and medicinal food.

• Proceedings of the Botanical Society of Korea Conference
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• 1987.07a
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• pp.133-148
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• 1987

Gibberellin(GA) 3-$\beta$ hydroxylation is very important for the shoot elogation in the higher plants, since only 3$\beta$-hydryoxylated GAs promote shoot elogation in several plants. Fluctuation of 3$\beta$-hydryoxylase activity was examined during seed maturation using two cultivars of , P. vulgaris, Kentucky Wonder (normal) and Masterpiece (dwarf). Very immature seeds of both cultivars contain high level of 3$\beta$-hydroxylase activity (per mg protein). Both cultivars showed maximum of enzyme activity (per seed) in the middle of their maturation process. Gibberellin 3$\beta$-hydroxylase catalyzing the hydroxylation of GA20 to GA1 was purified 313-fold from very early immature seeds of P. vulgaris. Crude soluble enzyme extracts were purified by 15% methanol precipitation, hydrophobic interaction chromatogrphy, DEAE ion exchange column chromatography and gel filtration HPLC. The 3$\beta$-hydroxylase activity was unstable and lost much of its activity duting the purification. The molecular weight of purified enzyme was extimated to be 42, 000 by gel filtration HPLC and SDS-PAGE. The enzyme exhibited maximum activity at pH 7.7. The Km values for [2.3-3H] GA20 and [2.3-3H]GA9 were 0.29 $\mu$M and 0.33 $\mu$M, respectively. The enzyme requires 2-oxoglutarate as a cosubstrate; the Km value for 2-oxoglutarate was 250 $\mu$M using 3H GA20 as a substrate. Fe2+ and ascorbate significantly activated the enzyme at all purification steps, while catalase and BSA activated the purified enzyme only. The enzyme was inhibited by divalent cations Mn2+, Co2+, Ni2+, Cu2+, Zn2+, Cd2+ and Hg2+. Effects of several GAs and GA anaogues on the putrified 3$\beta$-hydroxylase were examined using [3H]GA9 and GA20 as a substrates. Among them, GA5, GA9, GA15, GA20 and GA44 inhibited the enzyme activity. [13C, 3H] GA20 was converted by the partially purified enzyme preparation to [13C, 3H]GA1, GA5 and GA6, which were identified by GC-MS, GA9 was converted only GA4, GA15 and GA44 were converted to GA37 and GA38, respectively. GA5 was epoxidized to GA6 by the preparation. This suggests that 3$\beta$-hydroxylation of GA20 and epoxidation of GA5 are catalyzed by the same enzyme in P, vulgaris.

• 한국태양에너지학회:학술대회논문집
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• 2009.11a
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• pp.56-61
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• 2009

Paradigm depending only on fossil fuel for building heat source is rapidly changing. Accelerating the change, as it has been known, is obligation for reducing green house gas coming from use of fossil fuel, i.e. reaction to United Nations Framework Convention on Climate Change. In addition, factors such as high oil price, unstable supply, weapon of petroleum and oil peak, by replacing fossil fuel, contributes to advance of environmental friendly renewable energy which can be continuously reusable. Therefore, current new energy policies, beyond enhancing effectiveness of heat using equipments, are to make best efforts for national competitiveness. Our country supports 11 areas for new renewable energy including sun light, solar heat and wind power. Among those areas, ocean thermal energy specifies tidal power generation using tide of sea, wave and temperature differences, wave power generation and thermal power generation. But heat use of heat source from sea water itself has been excluded as non-utilized energy. In the future, sea water heat source which has not been used so far will be required to be specified as new renewable energy. This research is to survey local heating system in Europe using sea water, central solar heating plants, seasonal thermal energy store and to analyze large scale central solar heating plants in German. Seasonal thermal energy store necessarily need to be equipped with large scale thermal energy store. Currently operating central solar heating system is a effective method which significantly enhances sharing rate of solar heat in a way that stores excessive heat generating in summer and then replenish insufficient heat for winter. Construction cost for this system is primarily dependent on large scale seasonal heat store and this high priced heat store merely plays its role once per year. Since our country is faced with 3 directional sea, active research and development for using sea water heat as cooling and heating heat source is required for seashore villages and building units. This research suggests how to utilize new energy in a way that stores cooling heat of sea water into seasonal thermal energy store when temperature of sea water is its lowest temperature in February based on West Sea and then uses it as cooling heat source when cooling is necessary. Since this method utilizes seasonal thermal energy store from existing central solar heating plant for heating and cooling purpose respectively twice per year maximizing energy efficiency by achieving 2 seasonal thermal energy store, active research and development is necessarily required for the future.

• Journal of the Korean Geotechnical Society
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• v.28 no.9
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• pp.5-15
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• 2012

Field monitoring data for embankments in thirteen road construction sites at coastal area of the Korean Peninsula were analyzed to investigate the characteristics of lateral flow in soft grounds, to which vertical drain methods were applied. First of all, the effect of the embankment scale on the lateral flow was investigated. Thicker soft soils and lager relative embankment scale produced more horizontal displacements in soft grounds. Especially, if thick soft grounds were placed, the relative embankment scale, which was given by the ratio of thickness of soft ground to the bottom width of embankments, became larger and in turn large horizontal displacement was produced. And also higher filling velocity of embankments induced more horizontal displacements in soft grounds. The other major factors affecting the lateral flow in soft ground were the thickness and undrained shear strength of soft grounds, the soil modulus and the stability number. Maximum horizontal displacement was induced by less undrained shear strength and soil modulus of soft grounds. Also more stability numbers produced more maximum horizontal displacements. When the shear deformation does not develop, the stability number was less than 3.0 and the safety factor of bearing was more than 1.7. However, if the stability number was more than 5.14 and the safety factor of bearing was less than 1.0, the unstable shear failure developed in soft ground. 50mm can be recommended as a criterion of the allowable maximum horizontal displacement to prevent the shear deformation in soft ground, while 100mm can be recommended as a criterion of the allowable maximum horizontal displacement to prevent the shear failure in soft ground.

• Korean Journal of Construction Engineering and Management
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• v.19 no.3
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• pp.89-96
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• 2018

This study derived big issues in overseas construction through big data analysis. To derive big issues in overseas construction, candidate groups of big issues were identified through big data analysis targeting 53,759 issues including 39,436 issues from major portal sites, 10,387 issues from daily newspapers, and 336 issues in construction magazines from Oct. 1, 2016 to Sep. 30, 2017. The main results are as follows: First, the main issues of overseas construction for the past one year showed that markets were concentrated in Middle East Asia and most of them were low-price order plant projects, which revealed the limitations. Although orders of overseas construction were slightly upward in the first half of 2017 compared to previous year, overseas construction orders are still unstable due to uncertainties in the international affairs and drops in oil prices. Second, the interest topics based on the 8th core keywords of overseas construction among the overseas construction issues for the past one year showed that region (29.9%), corporation environment (22.0%), profitability (17.0%), organizations (15.1%), projects (5.2%), market environment (3.6%), policy and system (3.6%), and education (3.5%) in the order of interest. Third, 10 core issues that have expandability and persistence of discourse were extracted out of 30 issue candidates with regard to eight keywords. Based on the extracted issues, detailed analysis on each of the core issues in overseas construction and correlation analysis between 10 core issues were conducted.

• Journal of Korean Society of Forest Science
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• v.101 no.1
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• pp.130-137
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• 2012

This study has conducted to develop new gabion wall systems with vegetation base materials for stream bank stability and rapid rehabilitation. Vegetation base materials are primarily compounded with fine soil, organic composts and peat moss as plant fibers, a water retainer and a soil improver. Normally gabion wall systems resist the lateral earth pressures or stream power by their own weight. Therefore, fill material must have suitable weight, compressive strength and durability to resist the loading, as well as the effects of water and weathering. In this project, 100 to 200-mm clean, hard stones are basically specified, and about 50-mm rubbles are also used. Test application of new gabion wall system carried out in the stream bank of a small stream in the Gwangreung experimental forest, belonging to Korea Forest Research Institute (KFRI) in December 16th, 2006. As a result of the analysis of hydraulic stability of new gabion wall system, gabion wall system has highest threshold shear stress when the gabion wall covered by vegetation. New gabion wall system is highly resistant to sliding and overturning because safety coefficients exceed 1.5. As a result of term of slope stability analysis of new gabion wall system by Bishop and Fellenius methods, stability of stream bank was highly increased after the construction of gabion wall. Therefore, new gabion wall system is effective to stabilize unstable stream bank.

• Journal of Life Science
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• v.29 no.2
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• pp.223-230
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• 2019

Ozone is a gaseous molecule able to kill microorganisms, such as yeast, fungi, bacteria, and protozoa. However, ozone gas is unstable and cannot be used easily. In order to utilize ozone properly and efficiently, plant oil can be employed. Ozone reacts with C-C double bonds of fatty acids, converting to ozonized oil. In this reaction, ozonide is produced within fatty acids and the resulting ozonized oil has various biological functions. In this study, we showed that ozonized oil has antimicrobial activity against fungi and bacteria. To test the antimicrobial activity of ozonized oil, we produced ozonized olive oil. Ozonized olive oil was applied to Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. Antimicrobial activity was assayed using the disk diffusion method following the National Committee for Clinical Laboratory Standards. Minimal inhibitory concentrations (MIC) were 0.25 mg for S. aureus, 0.5 mg for S. epidermidis, 3.0 mg for P. aeruginosa, and 1.0 mg for E. coli. Gram positive bacteria were more susceptible than Gram negative bacteria. We compared growth inhibition zones against S. aureus and MRSA, showing that the ozonized olive oil was more effective on MRSA than S. aureus. Furthermore, the ozonized olive oil killed C. albicans within an hour. These data suggested that ozonized olive oil could be an alternative drug for MRSA infection and could be utilized as a potent antimicrobial and antifungal substance.