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http://dx.doi.org/10.3746/jkfn.2006.35.2.132

Antithrombin and Thrombosis Prevention Activity of Buckwheat Seed, Fagopyrum esculentum Moench  

Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University)
Kwon, Chong-Suk (Dept. of Food and Nutrition, Andong National University)
Son, Kun-Ho (Dept. of Food and Nutrition, Andong National University)
Kwon, Gi-Seok (The School of Bioresource Sciences, Andong National University)
Ryu, Hee-Young (Dept. of Food and Nutrition, Andong National University)
Kum, Eun-Joo (Dept. of Food and Nutrition, Andong National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.35, no.2, 2006 , pp. 132-138 More about this Journal
Abstract
Direct thrombin inhibitor, which is effective to prevent or cure the thrombosis, has been investigated in worldwide. In this study, we tried to screen antithrombosis agent from edible or medicinal plant. A strong antithrombin activity was identified from methanol or $95\%$ ethanol extract of buckwheat seeds. The solvent fractionation of buckwheat extracts using hexane, ethylacetate, butanol revealed that the butanol fraction has a prominent antithrombin activity. Thrombin time (blood-clot formation time) exceeded to over $2,000\%$ by addition of the butanol fraction at concentration of $312.5{\mu}g/mL$, whereas thrombin time extended to $336\%$ by addition of aspirin at concentration of $1,500{\mu}g/mL$. The butanol fraction showed anthrone-positive and ninhydrine-negative reaction. The active components were heat-liable, acid-unstable non-proteinous macromolecules (>30 KD). In vivo analysis using ICR male mouse showed that the buckwheat extract was superior than the aspirin in pulmonary thrombosis, KCN-induced coma and death. Our results suggest that the buckwheat is a potential as an antithrombosis agent and medicinal food.
Keywords
antithrombosis; buckwheat seed; Fagopyrum esculentum Moench; KCN-induced coma and death; pulmonary thrombosis;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
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