• Korean Chemical Engineering Research
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• 제46권2호
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• pp.376-382
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• 2008

안전성 향상을 위한 노력에도 불구하고 연구실험실에서의 화재, 폭발 등 각종 사고가 지속되고 있으며, 이에 따른 인명 및 재산 손실 또한 상당한 수준에 이르고 있다. 실험실 사고는 비슷한 유형의 사고가 주기적으로 반복되는 특성을 가지고 있으며, 안전성 향상을 위한 예방적 차원의 안전관리 체계가 절실하게 요구된다. 연구실험실 종사자들은 앞으로 국가의 과학기술 발전에 이바지할 젊은 과학자들로서 국가적 관점에서 상당한 손실이 아닐 수 없다. 따라서 본 연구에서는 과거의 사고 및 연구실 안전관리에 대한 연구의 분석결과를 기반으로 연구실험실에 상존하는 잠재적 위험성 저감 및 안전성 향상을 위해 요구되는 안전관리 중점요소들을 파악하였다. 궁극적으로 근본적인 실험실 안전성 향상을 위해 실험실 안전관리 매뉴얼, 실험실과 관련한 관리 시스템, 교육관리 시스템, 화학물질관리 시스템 및 실험실 자체안전점검 시스템으로 이루어진 화학 및 화학공학 실험실에 적합한 실험실 안전관리 시스템을 제안하여 실험실의 안전성 향상을 꾀하고자 하였다.

• Journal of Ginseng Research
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• 제48권3호
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• pp.298-309
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• 2024

Background: 20(S)-ginsenoside Rh2(GRh2), an effective natural histone deacetylase inhibitor, can inhibit acute myeloid leukemia (AML) cell proliferation. Lactate regulated histone lactylation, which has different temporal dynamics from acetylation. However, whether the high level of lactylation modification that we first detected in acute promyelocytic leukemia (APL) is associated with all-trans retinoic acid (ATRA) resistance has not been reported. Furthermore, Whether GRh2 can regulate lactylation modification in ATRA-resistant APL remains unknown. Methods: Lactylation and METTL3 expression levels in ATRA-sensitive and ATRA-resistant APL cells were detected by Western blot analysis, qRT-PCR and CO-IP. Flow cytometry (FCM) and APL xenograft mouse models were used to determine the effect of METTL3 and GRh2 on ATRA-resistance. Results: Histone lactylation and METTL3 expression levels were considerably upregulated in ATRA-resistant APL cells. METTL3 was regulated by histone lactylation and direct lactylation modification. Overexpression of METTL3 promoted ATRA-resistance. GRh2 ameliorated ATRA-resistance by downregulated lactylation level and directly inhibiting METTL3. Conclusions: This study suggests that lactylation-modified METTL3 could provide a promising strategy for ameliorating ATRA-resistance in APL, and GRh2 could act as a potential lactylation-modified METTL3 inhibitor to ameliorate ATRA-resistance in APL.

• 한국안전학회지
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• 제28권3호
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• pp.88-94
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• 2013

In university laboratories, areas of studies are becoming diverse and complicated according to the development of the industry. New forms of potential risk factors are increasing and they are unlike existing ones. In addition, many students are conducting various experiments in the laboratory. Therefore, they could be exposed to risk more often. Despite these risks, people do not recognize university lab safety activities properly and observe safety precautions. They are exposed to various laboratory accidents continually. In this study, we do not apply the present diagnosis method, checklist, but the safety assessment that is widely used in industry. Then we can find lots of hazard that checklist method could miss. This study will use the 4M and Hazard & Operability to design a new Laboratory safety assessments method.

• Safety and Health at Work
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• 제13권2호
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• pp.155-162
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• 2022

Background: Workers are exposed to several risks in academic laboratories due to the presence of potentially hazardous substances. The main objective of this study was to assess the prevalence of accident occurrence and associated risk factors among laboratory workers at the scientific laboratories of the public university in Lebanon and the impact of safety measures training and availability. Methods: In this observational study, a survey was conducted for one year in scientific laboratories at faculties of the public university. Results: Among the participants (N = 220), 45.0% have had accidents; the main cause was exposure to chemicals (73.7%) and more specifically by inhalation (45.4%). Females (85.9%) were more exposed to accidents than males. Laboratory workers with a master's degree, a full-time schedule, and more than ten years of experience were significantly more exposed to accidents (p < 0.05). A significant association was found between accident occurrence and training on management of hazardous products (p = 0.044), risks related to workplace (p = 0.030), eyewash and emergency shower (p < 0.001), first aid (p = 0.012), and facial protection availability (p = 0.019). In spite of the lack of safety culture and efficient training on laboratory safety, participants have shown a very good perception regarding safety measures to be applied in case of work accidents. Conclusion: Based on our findings, the prevalence of accident occurrence is elevated among lab workers at the public university. The impact of regular training on laboratory safety preventive measures is of great importance to ensure the efficiency of occupational health and safety in scientific laboratories.

• 한국환경보건학회지
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• 제31권5호
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• pp.365-371
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• 2005

This study was peformed to explore the current situation of university environmental health and safety (EHS) system and evaluate the effects of such system on university EHS practices. Essential elements for the university EHS system were identified based on the representative examples of foreign universities, and Hey were incorporated into a questionnaire, which was used in this study. Among the academic institutions we surveyed in this study, 89% of the universities employed health and safety manager, and 65% kept departments. However, less than 50% of universities maintained EHS policies, maintained health and established safety committees within the university headquarters, and held health and safety meetings on regular basis. Several basic requirements such as chemical hygiene program and laboratory safety training program lacked in many universities. Some basic surveillance was performed in many universities, however, only few universities carried out surveillance on biological safety, medical examination, and chemical exposure monitoring. EHS system had significant influence on university EHS program (p<0.05). Performances of EHS activities were influenced by presence of EHS policies, and university health and safety committees.

• 대한안전경영과학회지
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• 제19권4호
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• pp.133-140
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• 2017

The Ministry of Science, ICT and Future Planning made law for Pre-Hazard Risk Analysis in December 31, 2014 to protect researchers from continuing accidents in laboratory. Conducted before an experiment, Pre-Hazard Risk Analysis finds hazards of the experiment and rules to manage the hazards.So the Pre-Hazard Risk Analysis can support laboratory safety system by prevent accidents in laboratory. Pre-Hazards Risk Analysis is newly created system so that executors need Guidelines to perform this analysis properly. This study is to develop guide tool for Pre-Hazard Risk Analysis by analyzing other risk assessment systems; PSM, Off-site Consequence Assessment, laboratory safety system. Also, this study suggested how to establish database for Pre-Hazard Risk Assessment by analyse KRAS.

• 한국안전학회지
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• 제25권1호
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• pp.72-78
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• 2010

Laboratory accidents have happened incidently. However, laboratory safety management has been out of concerns for a long time. At last, the Korean government established an act called Establishment of safe environment of laboratories in 2005. To support the act and to establish policies on laboratory safety management，a national-wide survey was conducted for the 74 research organizations sampled and their 201 laboratories. For the survey, a safety management checklist with 114 items grouped into 20 categories was developed By using this checklist, safety expert groups investigated the laboratories and evaluated them on the five-point Likert scale. A statistical analysis was conducted to compare the safety management status between different research organization types and between different research fields. The resultsmanaw that the universities are in significant lower level on safety institution and safety education than the public research organizations and theare poration research organizations. In terms of research field, chemistry/chemical engineering laboratories generallymanaw higher level compared with theaothers. Conversely, biology/life science laboratories marked low levels in a number of checklist items.

• 한국안전학회지
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• 제34권1호
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• pp.115-121
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• 2019

Background: Due to the diversification and advancement of research, researchers have become to deal with a variety of chemical and biological harmful materials in the laboratories of universities and research institutes and the risk has increased as well. Therefore, it is necessary to strengthen the social safety net for laboratory accidents by strengthening the compensation to the level comparable to that of Korean Workers' Compensation & Welfare Service, when the researchers become physically disabled by laboratory accidents. The purpose of this study is to secure researchers' health rights and to create a research environment where researchers can work with confidence by strengthening the compensation to the level comparable to that of Korean Workers' Compensation & Welfare Service. Method: We analyzed the laboratory accidents by year, injury type, severity of accident and disability grade with the 6 year data from 2011 to 2016, provided by Laboratory Safety Insurance. Based on the analysis result, we predicted the financial impact on Laboratory Safety Insurance if we introduce a compensation annuity by disability grade which is similar to Injury-Disease Compensation Annuity of Korean Workers' Compensation & Welfare Service. Result :As of 2011, the insured number of Laboratory Safety Insurance was approximately 700,000. The Average premium per insured was KRW 3,339 and there were 158 claims. Total claim amount was KRW 130 million, whereas the premium was about KRW 2.3 billion. The loss ratio was very low at 5.75%. If we introduce a compensation annuity by disability grade similar to Injury-Disease Compensation Annuity of Korean Workers' Compensation & Welfare Service, the expected benefit amount for 1 case of disability grade 1 would be KRW 1.6 billion, assuming 2% of interest rate. Given current premium, the loss ratio, the ratio of premium income to claim payment, is expected 41.4% in 2017 and 151.6% in 2026. The increased loss ratio due to the introduce of the compensation annuity by disability grade is estimated to be 11.0% in 2017 and 40.4% in 2026. Conclusion: Currently, laboratories can purchase insurance companies' laboratory safety insurance that meets the standards prescribed by Act on the Establishment of Safe Laboratory Environment. However, if a compensation annuity is introduced, it would be difficult for insurance companies to operate the laboratory safety insurance due to financial losses from a large-scale accident. Therefore, it is desirable that one or designated entities operate laboratory safety insurance. We think that it is more desirable for laboratory safety insurance to be operated by a public entity rather than private entities.

• 한국축산식품학회지
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• 제38권2호
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• pp.291-301
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• 2018

To shorten the production cycle of Zaodan, this study first pickled Zaodan by a novel technology - vacuum decompression technology. Vacuum decompression technology could reduce the pickling time of Zaodan from 20 wk to about 9 wk. The protein content, moisture and pH of the Zaodan egg white gradually decreased with a concomitant increase in salt during the pickling process. The total sulfhydryl group (SH) group content of the egg white proteins was increased to $2.43{\times}10^{-3}mol/L$ after being pickled for 30 d, whereas the content of disulphide bonds (SS) was reduced to $23.35{\times}10^{-3}mol/L$. The surface hydrophobicity was lowest after pickling for 30 d. In addition, great changes occurred in the secondary structure of the egg white proteins after pickling for 20 d. The disappearance of ovomucin was noticeable based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis.

• 대한안전경영과학회지
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• 제17권3호
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• pp.97-104
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• 2015

The study is conducted in order to identify whether there is difference in laboratory safety management committee and safety manager appointment for type of institutes(university, research institute, enterprise institute). The study conducted statistical analysis of 2484 institutes (university, research institute, enterprise institute) that attended 'current status of laboratory safety management' by year(number of current status participation). As a result, university showed increase in forming safety management committee as the number of current status(year) increases. The number of safety management committee held were the highest amongst all institutes being 2 held/2 year. Furthermore, period of safety management committee held at university and research institutes are decreasing stately. For 2014, the rate of hosting periodic committee and temporary committee simultaneously when necessary are increasing. The rate of appointing safety manager who is in charge of laboratory safety is increasing in university, but it is decreasing in research institute and enterprise institute. The work pattern of safety manager in university is increase in exclusive duties and decrease in adjunct duties, while work pattern in research institute and enterprise institute is more on the contract duties.