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Comparison Analysis of Swine Gut Microbiota between Landrace and Yorkshire at Various Growth Stages (두 돼지 종의 다양한 성장단계에 따른 장내미생물 비교분석)

  • Unno, Tatsuya
    • Korean Journal of Microbiology
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    • v.50 no.4
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    • pp.308-312
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    • 2014
  • In this study, we conducted a next generation sequencing based microbial community analysis to investigate gut microbiota of the two commercially most available swine breeds, Yorkshire and Landrace. Bacterial 16S rRNA gene was amplified from fecal DNA using universal primer sets designed for V4 regions. Our comparison analysis of the gut microbiota of the two breeds suggested that their gut microbiota changed depending on the growth stages, while the difference between the two breeds was insignificant. However, there was a limited number of genera, the abundance of which was found to be different between the breeds. Those included the genus Xylanibacter in the Yorkshire samples, which was previously reported as a fiber digesting bacteria, likely increasing energy harvesting capacity of swine. In addition, others included opportunistic pathogens mostly found in the Yorkshire samples while the Landrace samples had significantly more prevalent Clostridium_IV species that were known to play a key role in systemic immunity of hosts. While microbial community shifts was found to be associated with growth stages, the difference between the two breeds seemed to be insignificant. However, there were several bacterial genera showing differential abundance, which may affect growth of hosts.

Geochemistry and Metamorphism of the Gneisses in Gwangyang-Hadong Area (광양-하동지역에 분포하는 편마암류의 지구화학 및 변성작용)

  • Park, Bae-Young;Suh, Gu-Won
    • Journal of the Korean earth science society
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    • v.29 no.3
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    • pp.221-245
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    • 2008
  • The precambrian granitic gneiss and porphyroblastic gneiss are widely distributed in the Gwangyang-Hadong area of Korea. This study focuses on the geochemical properties and metamorphic P-T conditions of these gneisses. These gneisses are plotted according to granodiorite domain on an IUGS silica-alkali diagram. Geochemical properties of major elements suggest that these rocks are of the sub-alkalic rock series, and were farmed from S-type magmas which were generated in a syn-collision tectonic environment. The amounts of trace elements (Zn, Sc, Sr, V, etc.) decreased as $SiO_2$ concentrations increased. Almandine and spessartine mol%'s and XFe are higher in garnet rims, while pyrope mol%'s are higher in the garnet cores. This seems to be the result of garnet growth and retrogressive metamorphism. Metamorphic zones are divided into sillimanite-cordierite, sillimanite, garnet, and biotite zones. Metamorphic P-T conditions estimated from the gneisses indicate high temperature and low to medium pressure metamorphism (689-757$^{\circ}C$, 5.0-5.6 kbar), followed by medium temperature, low pressure retrorade metamorphism (579-628$^{\circ}C$, 3.1-4.5 kbar), and overprinted retrogade metamorphism (502-558$^{\circ}C$, 1.6-2.3 kbar).

Effect of Setting on the Texture Intensity of Smoked Alaska Pollock Roe Sausage with Cellulose Casing and Its Quality Characteristics during Storage (셀룰로오스 케이싱에 충전한 명란훈연소시지의 텍스쳐에 대한 세팅의 영향 및 저장기간에 따른 품질특성)

  • Park, Jong-Hyuk;Kim, Young-Myung;Kim, Sang-Moo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.1
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    • pp.96-103
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    • 2006
  • Alaska pollock roe is mainly used as the production salted instead of salt-seasoned seafood (Myungranjeot). Alaska pollock roes with broken egg membrane are usually discarded as a waste product. In order to utilize the broken roes of Alaska pollock, imitated fish sausage was manufactured for commercial production. Hardness, cohesiveness, elasticity, brittleness, and gumminess of Alaka pollock roe sausage were evaluated based on mixture design and regression models. The higher amounts of carrageenan and tile lower amounts of starch caused the higher the texture intensity of Alaska pollock roe sausage. The pHs of control, vacuum and $N_2$ packages, increased up to 6.28, 6.23 and 6.24, respectively, during 4 months storage and then decreased. The values of volatile basic nitrogen (VBN), thiobarbituric acid (TBA), and total viable cell counts increased during storage periods, while the parameters were higher in control than in vacuum and Na packages. Coliform bacteria was not detected in all treatments during storage periods.

Monitoring of Veterinary Drug Residues in Foods Produced in Korea (국내 유통 식품의 잔류동물용의약품 모니터링)

  • Kim, Hee-Yun;Chung, So-Young;Choi, Sun-Hee;Lee, Jin-Sook;Choi, In-Sun;Cho, Min-Ja;Shin, Min-Su;Song, Jae-Sang;Choi, Jae-Chun;Park, Hee-Ok;Ha, Sang-Chul;Shin, Il-Shik;Seo, Eun-Chae
    • Korean Journal of Food Science and Technology
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    • v.42 no.6
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    • pp.653-663
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    • 2010
  • This study was conducted to monitor residues of 10 veterinary drugs in food products. Various veterinary drugs were examined including enrofloxacin, ciprofloxacin, norfloxacin, oxolinic acid, amoxicillin, ampicillin, oxytetracycline, tetracycline, chlortetracycline, and doxycycline in beef, pork, egg, chicken, eel, flatfish, and rockfish obtained from 6 different regions (Seoul, Incheon, Daejeon, Gwangju, Daegu, Busan). Residues were detected in 21 (6.5%) samples out of 321 samples. In particular, 2 (1.0%) livestock samples had detected residues among 203 products, and 19 (16.1%) aquaculture samples had residues detected among 118 products. The most frequently detected drug residues in aquaculture products were oxytetracycline and amoxicillin, but the levels were mostly below the MRL (Maximum Residue Limit). In only one flatfish sample, amoxicillin was found at a level higher than the MRL (0.05 mg/kg). In livestock products, residues of most veterinary drugs were not detected. But enrofloxacin was detected in 2 chicken (Korean name: Ogolgae) samples at a higher level than the MRL (0.1 mg/kg as the sum of ciprofloxacin).

Review of educational strategies to facilitate desirable attitudes toward gerontological nursing (노인간호에 대한 바람직한 태도형성을 위한 교육방안 고찰)

  • Yeom, Hyun-E
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.1
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    • pp.561-571
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    • 2016
  • Purpose: This study reviewed the innovative educational strategies that have been implemented successfully in US baccalaureate nursing education programs to facilitate appropriate attitudes about caring for older adults. Methods: The data were collected through a search of PubMed and CINAHL using the key words, gerontological or geriatric, attitudes about aging or older people, baccalaureate or undergraduate nursing education or curriculum. Results: The successful strategies are categorized as follows: 1) a multidisciplinary approach integrating aging issues and gerontological contents into diverse nursing courses, 2) active participation of community-dwelling healthy older individuals as an educational mentor, 3) use of audiovisual materials to investigate the misconceptions and attitudes about aging, and 4) discussion through critical thinking and self-reflection toward aging. Implications: For the interdisciplinary approach within nursing courses, it is essential to derive the key contents for gerontological nursing applicable to integration into diverse nursing courses. In addition, it is necessary to provide administrative support for implementing innovative strategies and constructing consistent partnerships with the community for active participation of the elderly as a mentor. Lastly, recognizing the significance of educational strategies for enhancing desirable attitudes toward gerontological nursing and supporting the development of educational capability of a faculty are key issues.

Spatio-temporal Variability of Phytoplankton Community in the Jeju Marine Ranching Area (JMRA) (제주 바다목장 해역 식물플랑크톤 군집의 시·공간적 변동 특성)

  • Yoon, Yang Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.11
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    • pp.7761-7772
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    • 2015
  • This study describes the spatio-temporal distributions in phytoplankton community such as species composition, standing crops and dominant species from April to November 2008 in the Jeju Marine Ranching Area (JMRA). A total of 106 species of phytoplankton belonging to 63 genera was identified. In particular, diatoms and dinoflagellates were occupied more than 61% and 35% of total species, respectively. The annual dominant species were Skeletonema costatum-like species(ls) in April, Torodinium teredo, Cylindrotheca closterium, Scrippsiella trochoidea in June, S. costatum - ls, Thalassionema nitzschioides, Pseudo-nitzschia pungens, Ebria tripartita in September and Corethron pennatum, Dictyocha fibura, Neoceratium teres in November. Phytoplankton cell density ranged between $0.6cells{\cdot}mL^{-1}$ in June and $64.0cells{\cdot}mL^{-1}$ in April. It fluctuated with an annual mean of $11.7cells{\cdot}mL^{-1}$ between the lowest value of $0.9cells{\cdot}mL^{-1}$ in November and the highest value of $37.0cells{\cdot}mL^{-1}$ by S. costatum -ls in April. Diversity index in September was higher than diatom blooming seasons in April. According to the phytoplankton community structure, the biological oceanographic characteristics of the JMRA was characterized by nanoplankton during a year. That is, primary production is deemed to have a higher possibility of being adjusted by a reproduction by material cycle in the ecosystems than nutrients supply from the lands.

Relation amang Stress, Ego-Resilience, Self-Efficacy, Clinical performance in graduate medical student (의학전문대학원생의 스트레스, 자아탄력성, 자기효능감과 임상수행능력과의 관계)

  • Hong, Sun-Yeun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.12
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    • pp.5797-5804
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    • 2011
  • Recently, the medical education is undergoing system transition from a medical college to a medical graduate school and introduction of performance test in the doctor license state examination. Therefore, To deal with these challenges, it is needed that medical faculty prepare the education programs for considering student's personal characteristics and improving Clinical performance. So, The purpose of this study was to understand relation among Stress, ego-resilience, self-efficacy and Clinical performance in graduate medical student, and for this study, We conducted a survey of 97 medical graduate school students in D city during the period from August 19st to 20st. 2011. The study found a negative correlation between Stress and Ego-Resilience, a positive correlation of stress with confidence at low region in Self-Efficacy. Ego-Resilience correlates positively with CPX at low region in Clinical performance, Preference for difficulty level of the task at low region in Self-Efficacy correlates negatively with OSCE. OSCE and CPX at low region in Clinical performance are positively correlated. Therefore, Stress reduction of students can improve Self-Efficacy, Furthermore, improve ultimately Clinical performance. According to this study, To improve Clinical performance competence, student's personal characteristics should be considered and education programs for self-efficacy and ego-resilience advancement be reflected in curriculum.

Quality Characteristics of Sulgidduk Supplemented with Citrus Peel Powder (감귤과피분말을 첨가한 설기떡의 품질 특성)

  • Kim, Jung-Hyon;Kim, Min-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.7
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    • pp.993-998
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    • 2011
  • Citrus peel powder was used to substitute 2%, 4% and 6% rice flour for making sulgidduk. Proximate composition, sensory characteristics, and principle components of sulgidduk containing citrus peel were analyzed and compared with those of sulgidduk. Protein, fat, carbohydrate, ash, color b-value (yellowness index), springiness, and adhesiveness of sulgidduk all significantly increased, whereas color L- and a-values, hardness, cohesiveness, and chewiness of sulgidduk decreased with the addition of citrus peel powder. No significant differences were observed regarding the contents of moisture and carbohydrates, or fracturability in any of the samples. Substitution of rice flour with citrus peel powder showed acceptable sourness and bitterness sensory scores, which were comparable to sulgidduk. Principle components analysis revealed total variation of 94% in the main structured information: PC1 and PC2 showed 79.31% and 14.69% variation. CP-B (citrus peel powder 4%) and CP-C (citrus peel powder 6%) associated the strongest with PC1 and sulgidduk without citrus peel powder associated with PC2. Attributes associated strongly with PC1 were color, bitterness, adhesiveness, and sourness. Therefore, sulgidduk containing CP-B can be developed based on its favorable quality characteristics and sensory evaluation.

Influence of Amount of Pedigree Information and Parental Misidentification of Progeny on Estimates of Genetic Parameters in Jeju Race Horses (제주마 집단의 혈연 정보량과 정보 오류가 유전 모수 추정치에 미치는 영향)

  • Kim, Nam-Young;Lee, Sung-Soo;Yang, Young-Hoon
    • Journal of Embryo Transfer
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    • v.29 no.3
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    • pp.289-296
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    • 2014
  • The pedigree information and race records of 1,000 m finishing time of Jeju race horses at KRA were used to study the effect of amount of pedigree information and parental misidentification on the estimates of genetic parameters. The modified data sets were made at the range of 2.5 to 25% parental misidentifications or loss of parental information of individuals with an increment of 2.5 percent. For each incremental level, 20 randomly replicated data sets were obtained and analyzed by single-trait analysis with a DF-REML(AI) algorithm. As the rate of misidentification increased or the amount of pedigree information decreased, the estimates of fraction of additive genetics variance component gradually decreased almost linearly (p<0.05), while the estimated fractions of error variance and permanent environmental variance components gradually increased for the finishing time. Regression coefficients of the percentage amount of both parents' information loss and incorrect pedigree information on additive genetic variances were -0.079 and -0.114, respectively (p<0.01). The estimate of heritability decreased by 0.92% for one percent loss of both parents' information and 1.39% for one percent increase of both parental misidentifications of progeny (p<0.01). For the consideration of probable incorrect and missing parent information of progeny in this early population of Jeju horses, the estimates of additive genetic parameters would be biased downward about ten percent. This results indicate that the amount of pedigree information loss and misidentification of progeny would severely affect estimates of genetic parameters and would reduce genetic gains for selection in Jeju horse population.

Spatio-temporal Distribution of Phytoplankton Community in the Jangsu Bay and Adjoining Sea of South Sea, Korea (장수만 식물플랑크톤 군집의 시.공간적 분포 특성)

  • Yoon, Yang Ho
    • Korean Journal of Environmental Biology
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    • v.32 no.1
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    • pp.75-87
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    • 2014
  • This study describes about the spatio-temporal distributions in phytoplankton community such as species composition, standing crop and dominant species from May 2006 to February 2007 in the Jangsu bay and the northwestern parts of Gamak bay. Based on the principal component analysis (PCA) of the environmental factors as well as biological parameters, the bio-oceanographical characteristics were analysed. A total of 83 species of phytoplankton belonging to 47 genera were identified. Whereas diatoms and dinoflagellates occupied more than 65% and 30% of total species, respectively. The annual dominant species were Chaetoceros affinis, Paralia sulcata and Bacillaria paxillifera in spring, Chaetoceros didymus, Ch. affinis and Octactis octonaria in summer, Skeletonema costatum-like species and B. paxillifera in autumn. Moreover phytoplankton cell density was ranged between 3.1 $cells{\cdot}mL^{-1}$ in spring and 521.0 $cells{\cdot}mL^{-1}$ in winter. It fluctuated with an annual mean of 76.0 $cells{\cdot}mL^{-1}$ between the lowest value of 7.6 $cells{\cdot}mL^{-1}$ in spring and the highest value of 220.2 $cells{\cdot}mL^{-1}$ by Skeletonema costatum-like species in winter. Briefly, the phytoplankton cell density in the mixing seasons was higher in comparison with the other seasons. According to the PCA, the biological oceanographic characteristics of the Jangsu bay was affected by the introduction of outside seawater particularly in temperature increasing seasons, and the other seasons, it may be described the light intensity, and mix between inner and outer bay sea waters.