• YAKHAK HOEJI
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• v.31 no.4
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• pp.224-229
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• 1987

The earthworm(Annelid) is a herbicine which has traditionally been used in the treatment of infectious fever, jaundice, infection of middle ear, laryngitis, pharyngitis, nephritis, headache, toothache and certain urinary tract infections from the olden times. Before the isolation and purification of biologically active components we analyzed the basic constituents(proteins, amino acids, mineral, etc.) with lyophilized powder of Lamnodrilus gotai Hatai. The results were as follows: Minerals detected and quantitatively analyzed were $Ca^#$, $Mg^#$, Fe, Zn, Mn, Cu, Co, Cr, Ni, Ge and Se. Amino acids detected were alanine, phenylalanine, leucine, glutamic acid, tyrosine, threonine, arginine, aspartic acid, methionine, lysine, serine, histidine, isoleucine, glycine, proline and etc. The constituents of proteins, fat, fiber, ash and phosphorous were measured. These constituents were compared and discussed with those of other investigations.

• Natural Product Sciences
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• v.11 no.1
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• pp.1-9
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• 2005

The chemical constituents from Hedyotis diffusa Willd and their pharmacological activities were summarized. It has been known data that this herb contains anthraquinones, terpenoids, steroids, flavonoids, organic acid, and polysaccharides. The studies of pharmacology have shown that Hedyotis diffusa Willd possess various levels of activities such as anticancer, anti-inflammatory, immunostimulatory, antioxidative, neuroprotective, and hepatoprotective activities.

• Korean Journal of Pharmacognosy
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• v.25 no.4 s.99
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• pp.342-347
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• 1994

Soybean and beanpaste have been used as ore of the important food in Korea. Beanpaste is made from soybean(seed of Glycine max $M_{ERRIL}$, Leguminosae) by traditional fermentation. It was expected that the secondary metabolites might be formed by the action of some microorganisms or enzymes on the constituents primarily contained in soybean during manufacturing or storage of beanpaste. A systematic investigation on the chemical constituents was carried out. As a result, sterol glycoside named daucosterin, lipo-daucosterin have been isolated from the lipid fraction soybean and their structures elucidated basis of chemical and physicochemical evidences. A ratio of composition of the lipid constituents in soybean and beanpaste were estimated.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 1997.10a
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• pp.111-124
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• 1997

With the combination of existing tidal predictio model and numerical tidal model, the efficient tidal prediction system was formulated and applied to the neighboring area of Pusan port. Because all tidal constituents for prediction (normally 69 constituents are used) can't be considered due to difficulties on computing efforts, some errors between the observed and predicted values were inevitably occurred. But it was confirmed that the practical results with about 10% of relative errors were obtained if four major tidal constituents(M$_2$, S$_2$. $K_1$, $O_1$) are used at least. Thus, if other constituents than four major tidal constituents are additornaly used, more accurate results will be obtained . Furthermore, if the databases of harmonic constants in coastal waters is made in advance using the numberical tidal model, prompt tidal prediction could be achieved whenever required.

• Natural Product Sciences
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• v.19 no.3
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• pp.236-241
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• 2013

A high-performance liquid chromatography-diode array detector (HPLC-DAD) method was established for quantitative evaluation of nine constituents of Fraxinus rhynchophylla such as four coumarins, esculin (1), fraxin (2), esculetin (3), fraxetin (4), three lignans, syringaresinol 4,4'-O-${\beta}$-diglucoside (5), pinoresinol 4-O-${\beta}$-glucoside (6), pinoresinol (9), one secoiridoid, oleuropein (7), and one coumarinolignan, cleomiscosin C (8). The preferred chromatographic condition was obtained on Phenomenex Gemini-NX (3 ${\mu}m$, C18 110A, $150{\times}4.60$ mm) and the mobile phase was composed of water and acetonitrile using a gradient elution. The wavelength was set at 220 nm. Extraction condition of these constituents in F. rhynchophylla was also optimized through extraction time, extraction solvent and extraction method using established method. From this study, extraction at $70^{\circ}C$ with the mixture of ethanol and water for more than 12 h was suggested to be good extraction condition for these constituents. Quantitation of nine constituents in different F. rhynchophylla samples was also successfully accomplished with the newly established method.

• Journal of Physiology & Pathology in Korean Medicine
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• v.26 no.3
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• pp.344-350
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• 2012

Previously, we showed that Dangguisoo-san (DGSS), an herbal formula that has been traditionally used for the treatment of blood stagnation, is also applicable for inflammatory lung diseases. Activation of Nrf2, an anti-inflammatory transcription factor, and suppression of NF-${\kappa}B$, a pro-inflammatory transcription factor, were suggested as an underlying mechanism. However, the constituents responsible for these activities remain unidentified. To this end, we prepared the water extracts of the 9 constituents of DGSS and tested for their effect on Nrf2 by using an Nrf2-Luciferase reporter cell line and western blot analysis. Results show that Carthamus tinctorius L.(CT), one of the 9 constituents of DGSS, strongly activated Nrf2. Similarly, when measured the effect of the 9 constituents on NF-${\kappa}B$ by using an NF-${\kappa}B$-Luciferase reporter cell line and western blotting for nuclear p65, indicative of activated NF-${\kappa}B$, most constituents were capable of suppressing NF-${\kappa}B$ in various degrees. However, CT and Cyperus rotundus L. (CR) strongly suppressed NF-${\kappa}B$ activity elicited by LPS. Of note, CT activated Nrf2 and suppressed NF-${\kappa}B$ strongly as well. Our results contributes to corroborating the anti-inflammatory effects of DGSS by identifying CT and CR as two major herbs responsible for activating Nrf2 and suppressing NF-${\kappa}B$. These results suggest that CT and CR represent some of the effects of DGSS in the regulation of inflammation.

• Journal of Astronomy and Space Sciences
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• v.26 no.4
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• pp.511-520
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• 2009

In this study, the Ocean Tide Loading (OTL) constituents were detected by the Precise Point Positioning (PPP) technique using GPS. Then, the GPS estimates of OTL constituents were compared with the predictions of the ocean tide models. We picked three permanent GPS stations as test sites and they are ICNW, SEOS, and CJUN. To detect the OTL constituents using GPS, we created vertical coordinate time series at 10-minute intervals using the PPP approach implemented in the GIPSY software. Through the tidal harmonic analysis of this height time series, the four major constituents ($M_2$, $S_2$, $K_1$, $O_1$) were determined. The amplitude obtained from the GPS height time series of the OTL constituents showed best match with the model predictions at CJUN, while the phase showed closest match at ICNW. The amplitude accuracy of the $M_2$, which is the dominant factor out of the 11 major constituents, was 24.8% on average.

• Journal of Korean Society on Water Environment
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• v.35 no.1
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• pp.55-63
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• 2019

The Four Major Rivers Restoration Project started in 2009 and completed in early 2013 is a large-scale inter-ministry SOC project investing ₩22.2 $10^{12}$ and one of the Project's objectives was to enhance the water-quality grade through recovering the river eco-system and environment. The average concentration and probability distribution of water-quality constituents at given and selected sampling sites are very significant elements for analyzing and controlling the water-quality of rivers or reservoirs effectively. Average concentration can be estimated by point estimator, distribution function of water-quality constituents or Bootstrap method, in which the distribution function estimated with more data in case of insufficient dataset, is applied. Ipo and Gangcheon water-quality monitoring stations in South Han River were selected to compare and analyze the variation of concentration before and after Ipo and Gangcheon Weirs construction, using the whole 4-year's data, from 2005 to 2008 and from 2014 to 2017. Water-quality constituents such as BOD and COD relating to oxygen demanding wastes and TP and Chlorophyll-a relating to the process of nutrient enrichment called eutrophication were also selected. The guidelines for water-quality control and management after weir construction including evaluation of water-quality constituents' variations can be presented by this paper.

• Natural Product Sciences
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• v.9 no.1
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• pp.22-27
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• 2003

This study was designed to investigate the effects of main constituents of Nigella sativa (NS) seed on the survival and CNS responses in experimental animals. The toxicological investigations were conducted for the determination of median lethal doses $(LD_{50})$ of NS seed constituents [i.e. aqueous extract (AE), fixed oil (FO), volatile oil (VO)] and main components of its VO [i.e. thymoquinone (TQ), ${\alpha}-pinene$ (AP) and p-cymene (PC)]. A part of this study includes evaluation NS constituents in the induction of minimal neurological deficit (MND) as a parameter for neurotoxicity using chimney test. In this study, the i.p. $LD_{50}$ values of AE, FO, VO, TQ (suspended In 0.5%CMC), TQ (dissolved in corn oil), AP and PC, were 3020, 3371, 1853, 616.6, 90.3, 1726 and 1523 mg/kg, respectively. All the NS constituents can be considered moderately toxic ($LD_{50}$ ranged from 616.6 to 3371 mg/kg), except the oily solution of TQ, which was very toxic ($LD_{50}$ was 90.3 mg/kg). It appeared that the toxicity of the whole VO is mainly due to its content of TQ and to some extent PC. All the NS constituents induced different degrees of MND at certain dose levels. The median neurotoxic (or sedating) doses $(TD_{50})$ of AE, FO, VO, TQ (suspended in CMC) and AP and PC, were 950, 1403, 306, 88.1, >173 and 368 mg/kg, respectively. TQ was the most potent component in inducing MND, whereas the FO and AE were the least. Neurotoxicity induced by the VO in the chimney test may refer basically to its contents of TQ and to some extent PC and AP.

• Asian-Australasian Journal of Animal Sciences
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• v.3 no.4
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• pp.293-298
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• 1990

Pathogen infections or mastitis inflammations usually develop differently on each udder of lactating cow. Although healthy udders will be attacked by the mastitis pathogens or the pathogens from blood in a long term, they would not be always inflamed. Somatic cell counts (SCC) in milk, which is utilized as an index of mastitis diagnosis, and the relation among SCC and milk constituents will have to be examined on each udder individually. Twelve cows of a Holstein cow herd in Nasu Research Station, which were suffering clinical or non-clinical mastitis, were selected, and SCC and milk constituents on each udder milk were measured. The effects of mastitis infection on udder milk components were relatively small except lactose content on udder milks of non-clinical mastitis (SCC< $10.0{\times}10^5$ per ml milk). On udder milks of clinical mastitis, however, high negative correlations were recognized between SCC and milk components. On different sampling days, high contents of fat and protein corresponded to that of total solids.