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Construction of Various Copy Number Plasmid Vectors and Their Utility for Genome Sequencing

  • Yang, Tae-Jin;Yu, Yeisoo;Frisch, David A.;Lee, Seunghee;Kim, Hye-Ran;Kwon, Soo-Jin;Park, Beom-Suk;Wing, Rod A.
    • Genomics & Informatics
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    • 제2권4호
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    • pp.174-179
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    • 2004
  • We developed various plasmid cloning vectors that are useful in the construction of genomic and shotgun libraries. Two medium copy vectors, pCUGlblu21 (pCb21) and pAGlblu21 (pAb21), which are resistant to kanamycin ($Km^R$) and chloramphenicol ($Cam^R$), respectively, are useful for cloning DNA inserts ranging from 5kb to 15kb. Two high copy vectors, pCUGlblu31 (pCb31) and pAGlblu31 (pAb31), containing $Km^R$ and $Cam^R$, respectively, are useful for DNA inserts less than 5kb. These vectors are well adapted for large-scale genome sequencing projects by providing choice of copy number and selectable marker. The small vector size is another advantage of these vectors. All vectors contain lacZa including multicloning sites that originated from pBluscriptllsk- for easy cloning and sequencing. Two medium copy vectors contain unique and rare cutting Swal (ATTTAAAT) restriction enzyme sites for easy determination of insert size. We developed two combined vectors, pC21A31 and pC31A21, which are combinations of (pCb21 + pAb31) and (pCb31 + pAb21), respectively. These two vectors provide four choices of vectors such as $Km^R$ and medium, $Cam^R$ and high, $Cam^R$ and medium, and $Km^R$ and high copy vectors by restriction enzyme cutting, dephosphorylation, and gel purification. These vectors were successfully applied to high throughput shotgun sequencing of rice, tomato, and brassica BAC clones. With an example of extremely biased hydro sheared 3 kb shotgun library of a tomato BAC clone, which is originated from cytogenetically defined peri-centromeric region, we suggest the utility of an additional 10 kb library for sequence assembly of the difficult-to-assemble BAC clone.

기후변화에 따른 농업용수공급패턴의 변화로 인한 이수안전도변화분석 (Water Supply Reliability Revaluation For Agricultural Water Supply Pattern Changes Considering Climate Changes)

  • 최영돈;안종서;신현석;차형선
    • 한국수자원학회:학술대회논문집
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    • 한국수자원학회 2010년도 학술발표회
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    • pp.273-277
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    • 2010
  • This research was performed to examine changes in the timing of the growth of crops along with changes in temperatures due tochanges and to analyze the change of water-supply-reliability by adding an analysis of the change of agricultural water supply patterns in the basin area of Miryang dam in Korea. Had-CM3 model from U.K. was the tool adopted for the GCM model, a stochastic, daily-meteorology-generation-model called LARS-WG was alsoused for downscaling and for the climate change scenario (A1B) which represents Korea's circumstances best. First of all, to calculate changes in the timing of the growth of crops during this period, the theory of GDD was applied. Except for the period of transplanting and irrigation, there was no choice but to find the proper accumulated temperature by comparing actual temperature data and the supply pattern of agricultural use due to limited temperature data. As a result, proper temperatures were found for each period. $400^{\circ}C$ for the preparation period of a nursery bed, $704^{\circ}C$ for a nursery bed's period, $1,295^{\circ}C$ for the rice-transplanting period, $1,744^{\circ}C$ for starting irrigation, and $3,972^{\circ}C$ for finishing irrigation. To analyze future agricultural supply patter changes, the A1B scenario of Had-CM3 model was adopted, and then Downscaling was conducted adopting LARS-WG. To conduct a stochastical analysis of LARS-WG, climate scenarios were generated for the periods 2011~2030, 2046~2065, 2080~2099 using the data of precipitation andMax/Min temperatures collected from the Miryang gauging station. Upon reviewing the result of the analysis of accumulated temperatures from 2011~2030, the supply of agricultural water was 10 days earlier, and in the next periods-2046~2065, 2080~2099 it also was 10 days earlier. With these results, it is assumed that the supply of agricultural water should be about 1 month ahead of the existing schedule to meet the proper growth conditions of crops. From the results of the agricultural water supply patterns should be altered, but the reliability of water supply becomes more favorable, which is caused from the high precipitation change. Furthermore, since the unique characteristics of precipitation in Korea, which has high precipitation in the summer, water-supply-reliability has a pattern that the precipitation in September could significantly affect the chances of drought the following winter and spring. It could be more risky to make changes to the constant supply pattern under these conditions due to the high uncertainty of future precipitation. Although, several researches have been conducted concerning climate changes, in the field of water-industry, those researches have been solely dependent on precipitation. Even so, with the high uncertainty of precipitation, it is difficult for it to be reflected in government policy. Therefore, research in the field of water-supply-patterns or evapotranspiration according to the temperature or other diverse effects, which has higher reliability on anticipation, could obtain more reliable results in the future and that could result in water-resource maintenance to be safer and a more advantageous environment.

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서울 다이나믹스 - 청계천 시점부 광장 설계 - (Seoul Dynamics - Cheonggyecheon Threshold Plaza Design -)

  • 김정윤;오피스 박김
    • 한국조경학회지
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    • 제34권1호
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    • pp.92-106
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    • 2006
  • The process of designing Cheonggyecheon Entrance Plaza began with researching four keywords: plaza, restoration. modernity and icon. The outcome of the research was reinterpreted into and informed the design. An urban plaza must not only be a stage for civic life but should also be a portrait of the city to which it belongs. Many Korean plazas, however, are treated as if they are parks. Yeouido Park, which was originally a vast urban void, and Seoul Plaza, recently paved with grass, are good example. The strong 'green myth' can hinder socio-political activities. Cheonggyecheon cannot be said to have been 'restored', since it is still disconnected from its origin and upper streams, and the water is circulated by electricity. So it is better understood as an artificial urban waterfront, rather than an ecologically restored stream. This fact might diminish its ecological value, but not its recreational one. The entrance plaza therefore should reflect that the new stream brings back an 'experience', not only water itself. At the same time, the catch phrase of this restoration project was 'post-modern'. The demolished Cheonggye Expressway represents the 'economy drive' of the 1970s, so the newly opened Cheonggyecheon serves as a perfect counterpart to it. But modernity in Korea is the spirit that made many of the good things, not only its shortcomings, we have now. And from the philosophy of this restoration project, we can see that it is still an ongoing attitude in a way. Remnant of Cheonggye Expressway can evoke our nostalgia for the era. There are plenty of symbols in Seoul, both as architecture and objects. But none of them provide citizens with experience, other than the experience of looking at them. Cheonggyecheon Entrance Plaza is a good place to serve as an icon for a dynamic Seoul. From the research, the designer concluded that this plaza should commemorate the incomparable horizontal experience of Cheonggyecheon and the old expressway, amid the vertical metropolis. The Pedestrian Sculpture, which people can stroll on and look out over Cheonggyecheon, is to be made of steel cladding with a core structure and represents the dynamism of the stream, Seoul and contemporary Korea. The choice of material and the steel structure are also ways of creating the icon. The Water Plaza, the space underneath the ramp, will accommodate people and their urban activities, providing an opportunity to play with water. The Waterblades will be a device for the dramatic beginning of the stream, simultaneously camouflaging ugly openings in the outlets. The Wall of Archaeology is to be made with pre-fab resin blocks, translucent enough so that people can see through any archaeological findings of the site. The strong water-resistant character of resin makes the wall steady throughout the flood season as well. Cheonggyecheon restoration project is an effort to combine contemporary urban demand with the once-existing physicality by evoking our nostalgia for it. The project itself shows many socio-political issues of present-day Korea. The entrance plaza design thus is focused on suggesting an icon for the metropolis, simultaneously celebrating the stream itself. Within this space, people will be exposed to a unique experience that any 'green myth' cannot offer.

Location Efficiencies of Host Countries for Strategic Offshoring Decisions Amid Wealth Creation Opportunities and Supply Chain Risks

  • Ma, Jin-Hee;Ahn, Young-Hyo
    • Journal of Korea Trade
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    • 제25권3호
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    • pp.21-47
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    • 2021
  • Purpose - Offshoring has emerged as one of the major trends in international trade and has become one of the strategies for achieving competitiveness in the global market. In spite of this, the expected gains of offshoring can be offset by hidden costs and risks, such as those associated with the COVID-19 pandemic, the trade war between the USA and China, and the ongoing trade dispute between Korea and Japan. To obviate such business failure and prevent critical business blunders, offshoring strategies that efficiently consider both risk elements and potential wealth creation are urgently need. The first purpose of this study is to contribute to the development of more advanced offshoring strategies to help host countries select the best locations to manage supply chain risks and create unique value. The second purpose is to specifically analyze the current status of Korea and provide Korean companies with implications to be considered when deciding whether to offshore or re-shore. Design/methodology - A Network DEA model was applied to measure the comparative location efficiency of national competencies for offshoring strategy from perspectives of wealth creation opportunities (profitability and marketability) and supply chain risk management. The location efficiencies are compared among a total 70 countries selected from the Global Competitiveness Index (GCI) and globally attractive locations outlined by Kearney (2017). For the secondary analysis of efficiency, a t-test examining the nature of competitive advantage and the level of sophistication in production processes was implemented in three divisions. We then analyzed differences in offshoring performance in terms of the identified national traits. Moreover, Tobit regression analysis is conducted to investigate the correlation between value-added business activities and each divisional efficiency, seeking to determine how each degree of value-added business activity influences the increase in offshoring productivity. Findings - Regarding overall location efficiency for offshoring performance, only the USA and Italy were identified as being efficient as host countries for offshoring, under circumstances of advanced development, such as productivity and risk management. Korea ranks 13th among 70 countries. The determinants of national competitiveness depend on national traits (the nature of competitive advantage and business sophistication). Countries with labor/resource advantages and labor-intensive industries are more competitive in terms of marketability than others. In contrast, countries with strong technology-intensive industries benefit offshoring companies, particularly in the technology sector, with the added advantage of supply chain risk management. As the perception of a value chain is broader in a country, it can achieve both production sophistication and competitive advantages such as marketability and SCRM. Originality/value - Existing studies focus on offshoring effectiveness from a company perspective. This paper contributes to comparing country efficiency in producing core competencies related to an offshoring strategy and also segments countries into three performance-based considerations associated with the global offshoring market. It also details Korea's position as an offshoring location according to national efficiency and competency.

한·중·일 전통주거의 재료적용 특성 비교 연구 - 각국 대표 상류주택을 중심으로 - (A Comparative Study on Application of Material in Traditional Residents of Korea, China and Japan - Focusing on Representative Upper-class House -)

  • 김휘경;최경란
    • 한국과학예술포럼
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    • 제19권
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    • pp.293-305
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    • 2015
  • 글로벌 시대의 대두와 동시에 각 나라 고유의 문화적 특성이 중요시 되는 현 시점에서, 자국의 문화정체성 확립은 이제 필수 불가결한 요소가 되었다. 이에 전통문화연구가 활발하게 이루어지고 있으며, 특히 주거건축의 연구는 총체적인 문화연구 지표로 활용될 수 있는 가치를 가지고 있다. 본 연구는 조형의 기본요소인 재료를 중심으로 실제적인 조사를 통해 한국 중국 일본의 전통건축의 적용 특성을 파악하여, 동아시아의 주거건축문화를 비교하는 동시에 우리나라만의 고유특성을 파악하는 데에 목적이 있다. 연구는 문헌조사와 현지답사를 함께 진행하였으며 연구의 대상이된 건축물은 한국(반가) - 무첨당, 중국(사합원) - 공왕부, 일본(쇼인츠쿠리) - 도큐도이다. 한·중·일의 건축 재료는 목재, 석재, 토재의 자연재료와 금속재, 지재, 기와, 전, 유리 등의 인공재료를 조합하여 건축을 구성하고 있으며, 이런 공통적 특징은 건축구성적인 부분에서 많이 나타나고 있다. 하지만 실내구성으로 갈수록 서로 다른 재료의 선택과 적용의 차이를 보이며 각 나라의 풍토, 가공방식, 생활문화에 맞는 재료를 선택하여 적용하고 있었다. 먼저 나라마다 식생과 기후에 영향을 받은 재료를 선택함으로써, 각 나라의 식생환경을 주거를 통해 확인 할 수 있었다. 또한 입식-좌식 문화의 특성, 실내구성에서 종이 마감 등 한국과 일본이 비슷한 양상을 보이는 반면, 중국은 확연히 차이를 보임을 알 수 있었다. 특히 재료의 가공과 관련해서 한국은 인위적 가공을 줄여 대체적으로 거칠고 투박한 질감을 그대로 느낄 수 있는 반면, 일본은 직재의 사용으로 정돈되고 세련된 느낌의 건축 표현이 이루어졌다. 중국은 세 나라 중에 재료의 인위적 가공이 가장 높았으며, 중국의 채색문화와 관련이 깊다는 것을 알 수 있다. 또한 벽돌, 유리 등 세부 건축 재료에 관련 기술들이 상당히 발전되어 있음을 알 수 있었다. 이는 '한국의 건축이 중국과 일본의 중간적 성격을 띄고 있다' 는 기존의 연구 결과와 상반되는 것으로 재료적 측면에 있어서는 자연미를 강조한 한국과 인위적인 미를 강조한 중국에 일본이 중간적 입장에서 재료를 적용한 모습을 알 수 있었다. 이렇듯 재료의 비교를 통해 한·중·일의 풍토성, 기능성, 심미성 등의 비물질적 요소가 주거와 어떤 관련을 가지고 적용되었는지 본 논문을 통해 확인 할 수 있다.

가전제품 소비자의 Channel Equity에 관한 탐색적 연구 (An Exploratory Study on Channel Equity of Electronic Goods)

  • 서용구;이은경
    • 마케팅과학연구
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    • 제18권3호
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    • pp.1-25
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    • 2008
  • 본 연구는 가전제품 소매채널에 관한 소비자의 선호 및 이용행태를 조사하고 가전제품 구매 채널 현황과 소비자들의 점포선택과 만족도를 분석하여 소비자가 특정 채널에 대하여 가지고 있는 소위 channel equity에 대하여 탐색적으로 접근 하고자 한다. 분석결과 가전제품 멀티채널 쇼핑환경은 소비자로 하여금 채널별로 차별화된 구매 패턴과 쇼핑동기를 만들어 주고 있었다. 백화점이나 대리점은 품질의 우수성과 A/S 측면에서 우세하며 대형할인점, 양판점, TV홈쇼핑, 인터넷쇼핑몰, 전자제품 판매 상가는 가격적인 측면이 경쟁 우위로 조사되었다. 채널별 소비자 만족도에 있어서는 애프터서비스가 잘되고 있는 백화점이나 대리점 등이 만족도가 상대적으로 높은 소매 채널임을 알 수 있다. 채널 에퀴티의 구성요인은 가격 경쟁력과 비교구매, 이용편리성, A/S, 판매원의 전문성, 배송의 신속성, 제품 검색용이, 판매원의 친절성, 매장의 쾌적성, 교통 편리성 등을 들 수 있다. 백화점의 경우 거의 모든 요소에서 가장 높은 만족도를 가지고 있어 채널 에퀴티가 높게 평가되었다. 인터넷 쇼핑몰은 제품을 쉽게 검색할 수 있다는 점에서 우위에 있으며 TV홈쇼핑의 경우는 비교구매와 가격경쟁력에서 우위가 있음을 알 수 있었고 채널별로 채널 에퀴티를 구성하는 요인들의 상대적 비중은 매우 달랐다. 본 연구에서는 채널 만족도를 평가한 후 에퀴티 포트폴리오와 채널별 에퀴티 구성 결과를 제시하고 있으나 향후 채널 에퀴티의 개념과 관리 툴에 대한 본격적인 연구가 요망된다.

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네트워크 분석을 활용한 딥러닝 기반 전공과목 추천 시스템 (Major Class Recommendation System based on Deep learning using Network Analysis)

  • 이재규;박희성;김우주
    • 지능정보연구
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    • 제27권3호
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    • pp.95-112
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    • 2021
  • 대학 교육에 있어서 전공과목의 선택은 학생들의 진로에 중요한 역할을 한다. 하지만, 산업의 변화에 발맞춰 대학 교육도 학과별 전공과목의 분야가 다양해지고 그 수가 많아지고 있다. 이에 학생들은 본인의 진로에 맞게 수업을 선택하여 수강하는 것에 어려움을 겪고 있다. 본 연구는 대학 전공과목 추천 모델을 제시함으로써 개인 맞춤형 교육을 실현하고 학생들의 교육만족도를 제고하고자 한다. 모델 연구에는 대학교 학부생들의 2015년~2017년 수강 이력 데이터를 활용하였으며, 메타데이터로는 학생과 수업의 전공 명을 사용했다. 수강 이력 데이터는 컨텐츠 소비 여부만을 나타낸 암시적 피드백 데이터로, 수업에 대한 선호도를 반영한 것이 아니다. 따라서 학생과 수업의 특성을 나타내는 임베딩 벡터를 도출했을 시, 표현력이 낮다. 본 연구는 이러한 문제점에 착안하여, 네트워크 분석을 통해 학생, 수업의 벡터를 생성하고 이를 모델의 입력 값으로 활용하는 Net-NeuMF 모델을 제시한다. 모델은 암시적 피드백을 가진 데이터를 이용한 대표적인 모델인 원핫 벡터를 이용하는 NeuMF의 구조를 기반으로 하였다. 모델의 입력 벡터는 네트워크 분석을 통해 학생과 수업의 특성을 나타낼 수 있도록 생성하였다. 학생을 표현하는 벡터를 생성하기 위해, 각 학생을 노드로 설정하고 엣지는 두 학생이 같은 수업을 수강한 경우 가중치를 가지고 연결되도록 설계했다. 마찬가지로 수업을 표현하는 벡터를 생성하기 위해 각 수업을 노드로 설정하고 엣지는 공통으로 수강한 학생이 있는 경우 연결시켰다. 이에 각 노드의 특성을 수치화 하는 표현 학습방법론인 Node2Vec을 이용하였다. 모델의 평가를 위해 추천 시스템에서 주로 활용하는 지표 4가지를 사용하였고, 임베딩 차원이 모델에 미치는 영향을 분석하기 위해 3가지 다른 차원에 대한 실험을 진행하였다. 그 결과 기존 NeuMF 구조에서 원-핫 벡터를 이용하였을 때보다 차원과 관계없이 평가지표에서 좋은 성능을 보였다. 이에 본 연구는 학생(사용자)와 수업(아이템)의 네트워크를 이용해 기존 원-핫 임베딩 보다 표현력을 높였다는 점, 모델을 구성하는 각 구조의 특성에 맞도록 임베딩 벡터를 활용하였다는 점, 그리고 기존의 방법론에 비해 다양한 종류의 평가지표에서 좋은 성능을 보였다는 점을 기여점으로 가지고 있다.

재망상복장구물중전자(在网上服装购物中电子)E-SERVQUAL 대전자충성도적영향(对电子忠诚度的影响) (The Effect of E-SERVQUAL on e-Loyalty for Apparel Online Shopping)

  • Kim, Eun-Young;Jackson, Vanessa P.
    • 마케팅과학연구
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    • 제19권4호
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    • pp.57-63
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    • 2009
  • 随着电子商务指数式增长(电子商务), 营销人员正试图获得通过强调服务质量和服务方面的相互作用而导致客户满意度或行为后果的竞争优势. 特别是对于服装, 服务质量是鼓励客户电子忠诚的关键因素之一, 并增加电子商务中服装零售的成功. 因此, 本研究探讨电子服务质量(电子SERVQUAL)的因素和根据Parasuraman等人(2005)的研究框架对网上服装购物的电子忠诚度的独特影响. 这项研究的具体目标是确定的基本层面的E - SERVQUAL, 分析有关在网上服装购物中电子SERVQUAL对点在忠诚度的影响的结构模型. 对于服务质量, 在网上购物方面的理论框架, 我们比较分析了有关传统和电子服务质量的因素的文献并确定了核心的两个方面和恢复服务. 这项研究的假设是电子SERVQUAL对电子忠诚度的影响. 客户满意度对电子服装网上购物服务的忠诚度有积极影响和客户满意度在电子SERVQUAL对服装网上购物电子忠诚度影响中的中介作用. 根据文献我们制成了一份自填式的问卷. 我们在美国从居住在大城市的有在网上购买服装产品的在线消费者中获得了252份有用的问卷. 通过因子分析(例如, 探索, 验证)评估了有效性和可靠性, 结构方程模型, 包括测量和结构模型, 通过线性结构8.8程序评估了包括测量和结构模型的结构方程模型. 结果显示, 网上购物网站的电子SERVQUAL的五个因素: 赔偿, 执行, 效率, 系统可用性和反应能力. 这支持在网上服装购物方面Parasuraman(2005)的E-S-QUAL包括两个方面的核心服务(如满足, 效率, 系统可用性)和恢复相关的服务(例如, 补偿, 回应). 在结构方程模型中, 有5个电子SERVQUAL因素的外生潜变量, 两个内生潜变量(例如客户满意度, 电子忠诚度). 对于测量模型, 各代表概念的因子载荷有显著的统计学意义, 比 0.60大, 内部一致性信从 0.85 至 0.8 8 不等. 在估计电子SERVEQUAL因素的结构模型, 系统的可用性对电子忠诚的直接和积极的作用, 而效率对网上服装购物的电子忠诚度有负面影响. 然而, 满足不是解释网上服装购物的电子SERVQUAL结果的重要指标. 这一发现意味着, 可被理解为系统提供服务的质量可能会增加对服装网上购物的客户满意度. 然而, 电子忠诚取决于服务质量没有被支持, 因为在网上服装购买中, 通过价值或满意度的中介作用, 服务质量对电子忠诚度(例如再购买意图)有间接影响. 此外, 获得赔偿和回应被发现对客户满意度有显著影响, 会影响网上服装购买的电子忠诚度. 因此, 获得赔偿和回应对电子忠诚度有显著的间接影响. 这表明, 恢复特定的服务因素在客户满意度最大化方面的重要作用, 以及维护客户对网上服装购物网站的忠诚度方面的重要作用. 研究结果在管理和研究方面都有启示. 时装营销者可以在建立与客户的长期关系的基础上, 不断测量顾客感知与恢复服务有关的服务质量等问题, 例如对问题和返回的迅速反应, 客户购买之后的问题赔偿. 为了保持电子忠诚, 在消费者购买服装中恢复服务对消费者第一次选择网站起到重要作用. 由于网上购物的消费者可以在任何地方购物, 提高竞争优势的营销策略是为消费者提供更好的服务质量, 最大限度地满意, 并把创造客户网上服装购买的电子忠诚度. 从研究的角度来看, 解释这些结果时, 本研究的一些局限需要被考虑. 对于未来的研究, 调查结果为这一重要主题理论和实证方面的进一步的研究提供了依据. 根据研究结果, 更全面的模型预测电子SERVQUAL的结果可以被开发和测试. 对全球时装市场, 这项研究可以扩展使用多国样本的跨文化的方法来研究服装的电子服务质量.

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명대의가(明代醫家)들의 두통(頭痛)에 대한 인식변화에 관한 연구 (The Historical Study of Headache in Chinese Ming Dynasty)

  • 전덕봉;맹웅재;김남일
    • 한국의사학회지
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    • 제24권1호
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    • pp.43-56
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    • 2011
  • Everyone once in a life experience headaches as symptoms are very common. According to a study in a country of more than a week and as many as those who have experienced a headache amounts to 69.4%. In addition, the high reported prevalence of migraine in 30s for 80% of all migraine sufferers daily life interfere with work or was affected. In Western medicine, the cause of headaches is traction or deformation of pain induced tissue like scalp, subcutaneous tissue, muscle, fascia, extracranial arteriovenous, nerves, periosteum. But it turns out there are not cause why pain induced tissue is being tracted or deformated. Therefore, most of the western-therapy is mainly conducted with regimen for a temporary symptom reduction. Therefore, I examined how it has been developed in Chinese Ming Dynasty, the perception of headache, change in disease stage and an etiological cause. Oriental medicine in the treatment of headache is a more fundamental way to have an excellent treatment. The recognition of head in "素問($s{\grave{u}}$ $w{\grave{e}}n$)" and "靈樞($l{\acute{i}}ng$ $sh{\bar{u}}$)" began to appear in 'Soul-神($sh{\acute{e}}n$) dwelling place' and 'where to gather all the Yang-'諸陽之會($zh{\bar{u}}$ $y{\acute{a}}ng$ $zh{\bar{i}}$ $hu{\grave{i}}$)'. Also, head was recognized as '六腑($li{\grave{u}}f{\check{u}}$) 淸陽之氣($q{\bar{i}}ng$ $y{\acute{a}}ng$ $zh{\bar{i}}$ $q{\grave{i}}$) and 五臟($w{\check{u}}$ $z{\grave{a}}ng$) 精血($j{\bar{i}}ng$ $xu{\grave{e}}$) gathering place'. More specific structures such as the brain is considered a sea of marrow(髓海-$su{\check{i}}$ $h{\check{a}}i$) in "內經($n{\grave{e}}i$ $j{\bar{i}}ng$)" and came to recognized place where a stroke occurs. Accompanying development of the recognition about head, there had been changed about the perception of headache and the recognition of the cause and mechanism of headache. And the recognition of headache began to be completed in Ming Dynasty through Jin, Yuan Dynasty. Chinese Ming Dynasty, specially 樓英($l{\acute{o}}u$ $y{\bar{i}}ng$), in "醫學綱目($y{\bar{i}}xu{\acute{e}}$ $g{\bar{a}}ngm{\grave{u}}$)", first enumerated prescription in detail by separating postpartum headache. and proposed treatment of headache especially due to postpartum sepsis(敗血-$b{\grave{a}}i$ $xu{\grave{e}}$). 許浚($x{\check{u}}$ $j{\grave{u}}n$) accepted a variety of views without impartial opinion in explaining one kind of headache in "東醫寶鑑($d{\bar{o}}ng-y{\bar{i}}$ $b{\check{a}}oji{\grave{a}}n)$" 張景岳($zh{\bar{a}}ng$ $j{\check{i}}ng$ $yu{\grave{e}}$), in "景岳全書($j{\check{i}}ng$ $yu{\grave{e}}$ $qu{\acute{a}}nsh{\bar{u}}$)", established his own unique classification system-新舊表裏($x{\bar{i}}nji{\grave{u}}$ $bi{\check{a}}ol{\check{i}}$)-, and offered a clear way even in treatment. Acupuncture treatment of headache in the choice of meridian has been developed as a single acupuncture point. Using the classification of headache to come for future generation as a way of locating acupoints were developed. Chinese Ming Dynasty, there are special treatments like 導引按蹻法($d{\check{a}}o$ y ${\check{i}}n$ ${\grave{a}}n$ $ji{\check{a}}o$ $f{\check{a}}$), 搐鼻法($ch{\grave{u}}$ $b{\acute{i}}$ $f{\check{a}})$, 吐法($t{\check{u}}$ $f{\check{a}}$), 外貼法($w{\grave{a}}i$ $ti{\bar{e}}$ $f{\check{a}}$), 熨法($y{\grave{u}}n$ $f{\check{a}}$), 點眼法($di{\check{a}}n$ $y{\check{a}}n$ $f{\check{a}}$), 熏蒸法($x{\bar{u}}nzh{\bar{e}}ng$ $f{\check{a}}$), 香氣療法($xi{\bar{a}}ngq{\grave{i}}$ $li{\acute{a}}of{\check{a}}$). Most of this therapy in the treatment of headache, it is not used here, but if you use a good fit for today's environment can make a difference.

스키마 관점에서 살펴본 인터넷 쇼핑몰 선택에 대한 소비자행동의 이해: Bricks & Clicks와 Pure-Player 인터넷 쇼핑몰 비교를 중심으로 (Empirical Analysis of Consumer Behavior on the Internet Shopping Mall Choice from the Schema Perspective: Comparison Between Bricks & Clicks and Pure-Player Shopping Mall)

  • 정남호;이건창
    • Asia pacific journal of information systems
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    • 제17권4호
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    • pp.165-186
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    • 2007
  • With the advent of a wide variety of Internet shopping malls, consumers can choose a best appealing shopping mall from among the Bricks-and-Clicks and Pure-Player malls. Pure-Players launched their operation grandiosely with the early stage of Internet use in 1995. However, after the burst of Dot-com company bubbles in 1997, Pure-Players introduce various types of business models to meet potential needs of consumers. While Pure-Players suffer skeptical views from market analysts as well as consumers, traditional offline companies learned important lessons from Dot-com companies collapse phenomena, and expanded their business channels into online in the name of Bricks-and-Clicks. Nowadays, Bricks-and-Clicks successfully establish in the market as one of reliable business partners among consumers. Therefore, it is no surprise that recent competitions between Bricks-and Clicks and Pure-Players become fiercer than ever to attract potential customers to their websites. In this situation, consumers can choose a shopping mall to their best satisfaction. Consumers can enjoy both offline and online options for shopping because Bricks-and Clicks provide both offline and online channels to consumers, which is compared with Pure-Players offering only online channel. Offline channel is unique in providing consumers with chances to touch and feel target products and services. Meanwhile, online channel is considered very viable and convenient shopping options for consumers. In this respect, it is easily assumed that consumers will show different online shopping behavior when they have to choose either Bricks-and-Clicks mall or Pure-Player mall for the sake of shopping. Remaining research issue in this case is how much consumers' schema would influence online shopping behavior between Bricks-and-Clicks and Pure-Players. Basically, schema is a framework for synthetic information recognition that individual consumers have and is very characteristic in that it focuses not on fragmentary facts but on the combination of various causes affecting results. Consumers' schema is closely represented by trust, structural assurance, and perceived relative advantage towards a specific type of shopping mall. In literature, there exist a lot of studies comparing Bricks-and-Clicks and Pure-Players. However, there is no study to pursue the analysis of consumer behaviors comparing Bricks-and Clicks and Pure-Players from the schema perspective. Therefore, this study aims to investigate this research gap. Empirical analysis is adopted by garnering valid questionnaires from 514 Internet shopping mall users. 237 were mainly using Bricks-and-Clicks for shopping, while 277 were found to visit Pure-Players for shopping. PLS was applied to analyze the survey data to verify the proposed research hypotheses. Findings from the empirical test results are as follows. First, consumers perceive more trust and relative advantage in Pure-Players, comparing with Bricks-and-Clicks. This result is against widely-accepted perception that Bricks-and-Clicks would be perceived by consumers as more trustworthy and relatively advantageous because they have offline reputation and stores. Therefore, it becomes more obvious that Internet is becoming daily necessaries, and consumers increasingly feel very comfortable in using the Internet for their own personal purposes. Second, consumers have firm faith in transaction safety, regardless Bricks-and-Clicks and Pure-Players. This seems due to the fact that most of shopping malls showing dubious transaction safety have no place in the market. In a nutshell, empirical results tell us that Pure-Players will grow very much in the future, to the extent that consumers perceive no difference in comparison with Bricks-and-Clicks. Besides, consumers' schema accumulated through trust and perceived relative advantage plays crucial role in determining consumer behavior.