• Journal of the Korean Professional Engineers Association
• /
• v.34 no.3
• /
• pp.10-15
• /
• 2001

The biotechnology is the study of biochemical and biological processes in living things. Since 1990's biotechnology has developed more repidly than all of the life technical industries, through the use of extremly sensitive analytical instruments, computers, and radioactive tracers. The biotechnician has been able to gain an understanding of the mechanisms and information on biotechnology, enables the biologist and biochemist to fine farther information about DNA techniques and human beings.

• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2004.10a
• /
• pp.201-201
• /
• 2004

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.8 no.6
• /
• pp.338-348
• /
• 2003

In the past decade, considerable progress has been made in developing the appropriate biotechnology for microalgal mass cultivation aimed at establishing a new agro-industry. This review points out the main biological constraints affecting algal biotechnology outdoors and the requirements for making this biotechnology economically viable. One of them is the availability of a wide variety of algal species and improved strains that favorably respond to varying environmental conditions existing outdoors. It is thus just a matter of time and effort before a new methodology like genetic engineering can and will be applied in this field as well. The study of stress physiology and adaptation of microalgae has also an important application in further development of the biotechnology for mass culturing of microalgae. In outdoor cultures, cells are exposed to severe changes in light and temperature much faster than the time scale re-quired for the cells to acclimate. A better understanding of those parameters and the ability to rapidly monitor those conditions will provide the growers with a better knowledge on how to optimize growth and productivity. Induction of accumulation of high value products is associated with stress conditions. Understanding the physiological response may help in providing a better production system for the desired product and, at a later stage, give an insight of the potential for genetic modification of desired strains. The potential use of microalgae as part of a biological system for bioremediation/detoxification and wastewater treatment is also associated with growing the cells under stress conditions. Important developments in monitoring and feedback control of the culture behavior through application of on-line chlorophyll fluorescence technique are in progress. Understanding the process associated with those unique environmental conditions may help in choosing the right culture conditions as well as selecting strains in order to improve the efficiency of the biological process.

• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2005.04a
• /
• pp.19-21
• /
• 2005

• The Microorganisms and Industry
• /
• v.12 no.1
• /
• pp.28-34
• /
• 1986

Nucleic acids do not only carry the genetic information, but are also the only substances being able of self-replication. Molecular cloning, an essential tool in biotechnology, requires among other things, an understanding of the mechanisms of replication which at present is fairly well known. After an introduction to the general principle, the status of art on replication procedure and its implication for biotechnology are dealt with.

• Proceedings of the Microbiological Society of Korea Conference
• /
• 2009.05a
• /
• pp.154-154
• /
• 2009

• Journal of Microbiology and Biotechnology
• /
• v.33 no.12
• /
• pp.1615-1624
• /
• 2023

Microcystis blooms threaten ecosystem function and cause substantial economic losses. Microorganismbased methods, mainly using cyanobactericidal bacteria, are considered one of the most ecologically sound methods to control Microcystis blooms. This study focused on gaining genomic insights into Paucibacter aquatile DH15 that exhibited excellent cyanobactericidal effects against Microcystis. Additionally, a pan-genome analysis of the genus Paucibacter was conducted to enhance our understanding of the ecophysiological significance of this genus. Based on phylogenomic analyses, strain DH15 was classified as a member of the species Paucibacter aquatile. The genome analysis supported that strain DH15 can effectively destroy Microcystis, possibly due to the specific genes involved in the flagellar synthesis, cell wall degradation, and the production of cyanobactericidal compounds. The pan-genome analysis revealed the diversity and adaptability of the genus Paucibacter, highlighting its potential to absorb external genetic elements. Paucibacter species were anticipated to play a vital role in the ecosystem by potentially providing essential nutrients, such as vitamins B₇, B₁₂, and heme, to auxotrophic microbial groups. Overall, our findings contribute to understanding the molecular mechanisms underlying the action of cyanobactericidal bacteria against Microcystis and shed light on the ecological significance of the genus Paucibacter.

• Journal of Microbiology and Biotechnology
• /
• v.8 no.2
• /
• pp.101-110
• /
• 1998

The extent of biological diversity being revealed by molecular techniques accentuates the need to develop methods to isolate and culture the large numbers of microorganisms that remain to be studied. The discovery and characterization of novel microorganisms will provide information useful in understanding microbial ecosystems and have the potential to lead to new products for the biotechnology industry. In this review, the use of innovative techniques and exploration of unusual ecosystems, that have begun to address the challenge of isolating the "uncultured" members of the microbial population, are examined.

• The Plant Pathology Journal
• /
• v.39 no.1
• /
• pp.1-20
• /
• 2023

In the past, rice bakanae was considered an endemic disease that did not cause significant losses in Korea; however, the disease has recently become a serious threat due to climate change, changes in farming practices, and the emergence of fungicide-resistant strains. Since the bakanae outbreak in 2006, its incidence has gradually decreased due to the application of effective control measures such as hot water immersion methods and seed disinfectants. However, in 2013, a marked increase in bakanae incidence was observed, causing problems for rice farmers. Therefore, in this review, we present the potential risks from climate change based on an epidemiological understanding of the pathogen, host plant, and environment, which are the key elements influencing the incidence of bakanae. In addition, disease management options to reduce the disease pressure of bakanae below the economic threshold level are investigated, with a specific focus on resistant varieties, as well as chemical, biological, cultural, and physical control methods. Lastly, as more effective countermeasures to bakanae, we propose an integrated disease management option that combines different control methods, including advanced imaging technologies such as remote sensing. In this review, we revisit and examine bakanae, a traditional seed-borne fungal disease that has not gained considerable attention in the agricultural history of Korea. Based on the understanding of the present significance and anticipated risks of the disease, the findings of this study are expected to provide useful information for the establishment of an effective response strategy to bakanae in the era of climate change.

• Journal of Embryo Transfer
• /
• v.29 no.1
• /
• pp.1-5
• /
• 2014

Recent 2 decades, including in vitro maturation (IVM), assisted reproductive technologies (ARTs) achieved noteworthy development. However the efficiency of ARTs with in vitro matured oocytes is still lower than that with in vivo oocytes. To overcome those limitations, many researchers attempted to adapt co-culture system during IVM and consequently maturation efficiency has been increased. The beneficial effects of applying co-culture system is contemplated base on communication and interaction between various somatic cells and oocytes, achievement of paracrine factors, and spatial effects of extracellular matrix (ECM) from somatic cell surface. The understanding of co-culture system can provide some information to narrow the gap between in vitro and in vivo. Here we will review current studies about issues for understanding cu-culture system with various somatic cells to improve in vitro maturation microenvironment and provide bird view and strategies for further studies.