• Journal of Microbiology and Biotechnology
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• 제26권10호
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• pp.1661-1667
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• 2016

The ginsenoside-hydrolyzing β-glucosidase gene (bgy2) was cloned from Lactobacillus brevis. We expressed this gene in Escherichia coli BL21(DE3), isolated the resulting protein, and then utilized the enzyme for the biotransformation of ginsenosides. The bgy2 gene contains 2,223 bp, and encodes a protein of 741 amino acids that is a member of glycosyl hydrolase family 3. β-Glucosidase (Bgy2) cleaved the outer glucose moieties of ginsenosides at the C-20 position, and the inner glucose at the C-3 position. Under optimal conditions (pH 7.0, 30℃), we used 0.1 mg/ml Bgy2 in 20 mM sodium phosphate buffer (PBS) for enzymatic studies. In these conditions, 1.0 mg/ml ginsenoside Rb1 and ginsenoside F2 were converted into 0.59 mg/ml ginsenoside Rd and 0.72mg/ml compound K, with molar conversion productivities of 69% and 91%, respectively. In pharmaceutical and commercial industries, this recombinant Bgy2 would be suitable for producting ginsenoside Rd and compound K.

• 터널과지하공간
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• 제25권6호
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• pp.527-533
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• 2015

본 연구에서는 고정익 무인항공기(드론, SenseFly eBee)를 이용하여 국내 대규모 석회석 노천광산에 대한 지형측량을 수행하였다. 비행고도 300 m, 비행속도 12 m/s 조건으로 약 30분간 자동모드 비행을 수행한 결과 현장에서 총 288장의 항공사진을 촬영할 수 있었다. 특이점 추출이 불가능한 37장의 항공사진을 제외한 251장의 항공사진 자료들을 보정하고, 정합한 결과 7 cm 해상도의 정사영상과 수치표면모델 자료를 생성할 수 있었다. 4곳의 지상기준점에 대하여 고정밀 위성측정시스템를 이용하여 측정한 위치 좌표와 고정익 무인항공기 사진측량시스템을 이용하여 추출한 위치 좌표를 비교한 결과 평균 제곱근 오차가 15 cm 내외로 분석되었다. 고정익 무인항공기는 회전익 무인항공기에 비해 상대적으로 비행시간이 길어 넓은 영역의 신속한 지형측량이 가능하므로 대규모 노천광산 현장에서 효과적으로 활용될 수 있을 것이다.

• Journal of Microbiology and Biotechnology
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• 제32권5호
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• pp.630-637
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• 2022

The objective of this study was to optimize industrial-grade media for improving the biomass production of Weissella cibaria JW15 (JW15) using a statistical approach. Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K₂HPO₄, potassium citrate, ⳑ-cysteine phosphate, MgSO₄, and MnSO₄) were screened by using the Plackett-Burman design. Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM). The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K₂HPO₄, 5.0 g/l sodium acetate, 0.1 g/l MgSO₄·7H₂O, 0.05 g/l MnSO₄·H₂O, and 1.0 g/l Tween 80. The OM significantly improved the biomass production of JW15 over an established commercial medium (MRS). After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l). Correspondingly, JW15 showed a rapid and increased production of lactic and acetic acid in the OM. To perform a scale-up validation, batch fermentation was executed in a 5-l bioreactor at 37℃ with or without a pH control at 6.0 ± 0.1. The biomass production of JW15 significantly improved (1.98 times higher) under the pH control, and the cost of OM was reduced by two-thirds compared to that in the MRS medium. In conclusion, OM may be utilized for mass producing JW15 for industrial use.

• 한국수자원학회논문집
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• 제56권12호
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• pp.969-980
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• 2023

2000년대 이후 우리나라에 도입된 앙상블 유량 예측은 수문 예측 현업에서 성과를 보였음에도 불구하고 수자원 시스템 관리에서는 적극적으로 활용되지 못하고 있다. 따라서 본 연구에서는 용수공급을 위한 수자원 관리 측면에서 앙상블 유량 예측의 효용성을 보이고자 댐 실무자들을 위한 간단한 가상 예제를 만들고, 이를 확장하며 실제 대한민국 다목적댐에 적용하였다. 가상 예제에서는 앙상블 유량 예측의 평균은 같지만 표준편차가 다를 때, 댐 운영 비용이 상이함을 확인하였다. 그리고 이를 대한민국 다목적댐에 적용하기 위해 동일한 평균을 가지지만 표준편차가 평균과 동일한 유형W와 표준편차가 평균의 10배인 유형 P를 가정하여 용량 대비 연유입량 비율에 따라 소양강댐(CIR = 1.345)과 충주댐(CIR = 0.563)에 표본 추계학적 동적계획법으로 최적방류량을 도출하였다. 그리고 도출된 최적방류량으로 2020년부 2022년까지 모의 운영을 진행하였다. 그 결과, 충주댐에서는 표준편차가 적은 유형W에서 상대적으로 공격적인 최적방류량이 도출되었고, 모의 운영 결과 또한 양호함을 보였다. 소양강댐에서도 최적방류량에서 충주댐과 같은 결과를 보였지만, 모의 운영에서는 유형W와 P의 차이가 없었다. 결과적으로 동일한 평균을 가지더라도 앙상블 유량 예측의 표준편차에 따라 다른 최적방류량과 모의 운영 결과가 도출되었고, 특히 용량 대비 연유입량이 작을수록 이러한 불확실성에 더욱 민감하게 반응함을 보였다. 본 내용을 바탕으로 현재 단일 대푯값만을 사용하여 댐 운영을 하는 수자원 관리에 개선을 기대한다.

• Journal of Microbiology
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• 제45권2호
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• pp.133-138
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• 2007

This study addresses the susceptibility of Spodoptera frugiperda (Sf9 and Sf21), Trichoplusia ni (Hi5), and S. exigua (Se301) cells to the Bombyx mori nucleopolyhedrovirus (BmNPV). Although these cells have classically been considered nonpermissive to BmNPV, the cytopathic effect, an increase in viral yield, and viral DNA synthesis by BmNPV were observed in Sf9, Sf21, and Hi5 cells, but not in Se301 cells. Very late gene expression by BmNPV in these cell lines was also detected via ${\beta}-galactosidase$ expression under the control of the polyhedrin promoter. Sf9 cells were most susceptible to BmNPV in all respects, followed by Sf21 and Hi5 cells in decreasing order, while the Se301 cells evidenced no distinct viral replication. This particular difference in viral susceptibility in each of the cell lines can be utilized for our understanding of the mechanisms underlying the host specificity of NPVs.

• 한국세라믹학회지
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• 제53권1호
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• pp.13-17
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• 2016

It is highlighted that increasing the adsorbent surface area on volumetric basis is very important in providing an easy access for gas molecules. Fine particles around $3{\mu}m$ of soft-burned dolomite kiln dust (SB-DKD) were hydrated to wet slurry samples by ball mill process and then placed in a chamber to use spray dryer method. Spherical granules with particle size distribution of $50{\sim}60{\mu}m$ were prepared under the experimental condition with or without addition of a pore-forming agent. The relationship between bead size of the pore-forming agent and size of SB-DKD particles is the most significant factor in preparation of spherical granules with a high porosity. Whereas addition of smaller beads than SB-DKD resulted in almost no change in the surface porosity of spherical granules, addition of larger beads than SB-DKD contributed to obtaining of the particles with both 15 times larger average pore volume and 1 order of magnitude larger porosity. It is considered that spherical granules with improved $N_2$ gas adsorption ability may also be utilized for other atmospheric gas adsorption.

• 산업기술연구
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• 제25권B호
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• pp.81-87
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• 2005

This project addresses the product identification and design application of corrugated products for kinetic architectural applications. Initially, an architectural approach to product identification was carried out with the end goal of demonstrating application design principles of numerous novel architectural examples from the forest products industry. A design and prototyping stage followed demonstrating such application design principles and functional analysis on various novel architectural examples. The results of coupling product identification with proven application advantages will be of potential value not only to architecture but also to other designers in the arts and sciences. The general motivation for the research arises out of a growing interest corrugated and paper products to be ideally suited for various architectural applications for their unparallel environmental benefits. Applications in architecture that use fewer resources and that adapt efficiently to complex and changing conditions are particularly relevant for an industry (architecture) that is increasingly aware of its environmental responsibilities. Corrugated and paper products are highly under-utilized by the building industry as design choice material for both interior and exterior applications. An increased awareness of the benefits of corrugated and paper products coupled with a process of designing for the total use and reuse will prove to be a valuable means by which issues of energy efficiency and environmental quality of buildings could be enhanced to be more efficient, affordable, and therefore reach a broader audience of users.

• 동력기계공학회지
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• 제16권2호
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• pp.30-35
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• 2012

The geothermal energy is one of the renewable energy sources which can contribute in accomplishing a vision and goal of the national plan on energy for a government suggestion. Especially, the geothermal energy is evaluated as the nearly unlimited resources. The yearly underground temperature distribution by depth is very important to the design of air-conditioning system which uses a geothermal energy. Furthermore, there has no data for comparisons to numerical analysis. In this study, the yearly underground temperature is measured under the depth of 2 m in Tongyeong, and these data are compared with numerical analysis results for checking the accuracy. The results showed that the experimental temperature and numerical results had a good agreements and these results will be utilized to predict a performance of air-conditioning system for using a geothermal energy.

• 한국농공학회논문집
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• 제54권2호
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• pp.15-26
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• 2012

For stable and sustainable crop production, understanding the effects of climate changes on agricultural water resources is necessary to minimize the negative effects which might occur due to shifting weather conditions. Although various studies have been carried out in Korea concerning changes in evapotranspiration and irrigation water requirement, the findings are still difficult to utilize fordesigning the demand and unit duty of water, which are the design criteria of irrigation systems. In this study, the impact analysis of climate changes on the paddy water demand and unit duty of water was analyzed based on the high resolution climate change scenarios (specifically under the A1B scenario) provided by the Korea Meteorological Administration. The result of the study indicated that average changes in the paddy water demand in eight irrigation districts were estimated as -2.4 % (2025s), -0.2 % (2055s), and 3.2 % (2085s). The unit duty of water was estimated to increase on an average within 2 % during paddy transplanting season and within 5 % during growing season after transplanting. This result could be utilized for irrigation system design, agricultural water resource development, and rice paddy cultivation policy-making in South Korea.

• KIEAE Journal
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• 제17권1호
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• pp.43-48
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• 2017

Purpose: Building owners or residents have concerns to strive for energy-saving and environmental conservation by utilizing with eco-friendlier energy resources for their physical environment. In this paper, an algae façade system is proposed as an energy-friendly building component to improve energy productivity and indoor environmental quality, and this study aims at verifying alternative technologies for implementing building elevations that contain various colors equipped with algae façade systems and suggesting design guidelines to enhance both building performance and design values. Method: The color of algae is basically ranged about the saturation green, and it is hardly converted to other variations. Such a problem can be resolved through the artificial lights like LED (Light Emitting Diode) lamps to mix the color from the algae and buildings could possibly change the elevation in many ways under the influence of daylight. Result: As a result, the suggested system may increase the aesthetic aspect of the building in response to environmental changes. The system cannot possibly be applied for only new construction, but also it can be utilized with the existing buildings as well. The proposed system is expected to be applied not only a new construction and any existing buildings as well, and it will cover from the environmentally friendly energy generation in the industry to a new application system for increasing energy efficiency and the beauty of building envelopes.