• Biomolecules & Therapeutics
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• 제24권1호
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• pp.75-84
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• 2016

This study was designed to investigate the cytoprotective effect of rosmarinic acid (RA) on ultraviolet B (UVB)-induced oxidative stress in HaCaT keratinocytes. RA exerted a significant cytoprotective effect by scavenging intracellular ROS induced by UVB. RA also attenuated UVB-induced oxidative macromolecular damage, including protein carbonyl content, DNA strand breaks, and the level of 8-isoprostane. Furthermore, RA increased the expression and activity of superoxide dismutase, catalase, heme oxygenase-1, and their transcription factor Nrf2, which are decreased by UVB radiation. Collectively, these data indicate that RA can provide substantial cytoprotection against the adverse effects of UVB radiation by modulating cellular antioxidant systems, and has potential to be developed as a medical agent for ROS-induced skin diseases.

• 생명과학회지
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• 제8권3호
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• pp.272-278
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• 1998

UV-B 강도 변화가 식물의 생장에 미치는 영향을 조사하기 위하여, 오이를 이용하여 3주간 3단계의 UV-B[일일 평균 UV-$B^{BE}$; 무처리(0.03), 저UV-B(6.40), 고UV-B(11.32) kJ $m^{-2}$]조사 실험을 수행하였다. 3주간의 UV-B 조사에 의해 건물중 및 엽면적은 고UV-B 처리와 저UV-B 처리에서 각각 60%와 40% 정도의 감소를 보였으나, 엽중비는 증가하였다. UV-B에 의해 고UV-B 처리에서 약 45%, 저UV-B 처리에서 약 37%의 광합성속도의 감소를 나타내었으나 증산속도의 변환느 나타나지 않았다. 총 엽록소 함량은 UV-B 강도의 증가에 따라 감소하였으며, UV-흡수물질인 flavonoid의 함량은 고UV-B 처리에서 약 19% 저UV-B처리에서 약 14%의 증가를 보였다. 이상의 결과로 볼 때 UV-B조사는 오이 식물에 극심한 생육 저해를 나타내었으며, 그 피해는 UV-B 강도에 따라 증가하였다. 따라서 현재 지구에 도달하는 태양UV-B에 의해서도 오이식물은 영향을 받고 있는 것으로 사료되며, 장래 예상되는 오존층 감소에 의해서도 생육장해를 나타낼 것으로 사료된다.

• 한국식품과학회지
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• 제29권2호
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• pp.355-361
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• 1997

털없는 쥐표피를 적출하여 ultraviolet B로서 조사한 후 녹차의 열수 추출물이 표피의 효소의 활성에 대한 항산화 효과를 조사하였다. 녹차의 열수추출물을 5, 25, 50 및 $100\;{\mu}g$으로 쥐표피에 첨가한 후 ultraviolet B을 $1.0\;joule/cm^{2}$로 조사한 후 효소활성을 조사한 결과 catalase와 glutathione reductase는 녹차의 열수 추출물의 첨가용량이 증가할수록 영향을 미치는 것으로 나타났으나 glutathione peroxidase와 superoxide dismutase는 영향을 받지 않았다. 한편 lipoxygenase의 활성을 알아보기 위하여 arachidonic acid에 $50\;{\mu}g$ 녹차의 열수 추출물을 첨가하여 대사를 검토한 결과 대사산물 12 또는 15-hydroxyeicosa- tetraenoic acid보다 5-HETE 및 8-HETE가 억제되었다. 녹차의 열수 추출물을 5, 25, 50 및 $100\;{\mu}g$ 첨가하였을 때 5-HETE는 각각 32, 52, 62 및 80% 억 제되었고 8-HETE는 각각 36, 47, 70 및 84%로 억제됨을 알 수 있었다.

• 생약학회지
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• 제34권2호통권133호
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• pp.156-160
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• 2003

$NF-{\kappa}B$ (nuclear factor-kappa B) plays a particularly central role in epidermal biology. It has been established that ultraviolet radiation (UVR) is one of the mechanisms to induce the activation of $NF-{\kappa}B$ in human skin. We previously demonstrated that melanogenic inhibitors may act through the inhibition of $NF-{\kappa}B$ activation in keratinocytes. In order to find another type of melanogenic inhibitors of $NF-{\kappa}B$ activation, various kinds of the extracts from crude drugs $(30\;{\mu}g/ml)$ were preincubated with transfectant HaCaT cells for 3 hrs and then UVR $(60\;mj/cm^2)$ was irradiated. UVR-exposed cells were incubated for another 6 hrs to measure the $NF-{\kappa}B$ activity. $NF-{\kappa}B$ activation was measured with the secreatory alkaline phosphates (SEAP) reporter gene assay using a fluorescence detection method. Among natural products, Lycium chinense, Acanthopanax senticosus, Angelica koreana, Kalopanax pictus and Asparagus cochinchinensis were the most potent inhibitors of $NF-{\kappa}B$ activation by UVR. These observations suggest that some crude drugs might act partially through the modulation of the synthesis of melanotrophic factors to decrease melanogenesis in keratinocytes.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2007년도 6th OSONG INTERNATIONAL BIO-SYMPOSIUM
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• pp.244.1-244.1
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• 2007

• Journal of Ginseng Research
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• 제30권4호
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• pp.188-193
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• 2006

We induced the activation of melanocytes in the epidermis of C57BL/6 mice by ultraviolet B (UVB) irradiation and observed the effect of red ginseng (RG) on the formation, and decrease of UVB-induced epidermal mel-anocytes. C57BL/6 mice were irradiated by UVB $80mJ/cm^2$ (0.5 mW/sec) daily for 7 days, and RG was intraperitoneally or topically applied pre- or post-irradiation. For the estimation of change of epidermal melanocytes, light microscopic observation with dihydroxyphenylalanine (DOPA) stain was performed. Split epidermal sheets prepared from the ear of untreated mice exhibited 11-16 $melanocytes/mm^2$, and one week after UV irradiation, the applied areas show an increased number of strongly DOPA-positive melanocytes with stout dendrites. But intraperitoneal or topical treatment with RG before each irradiation interrupted UVB-induced pigmentation and resulted in a marked reduction in the number of epidermal melanocytes as compared to radiation control skin. The number and size of DOPA-positive epidermal mel-anocytes were also significantly decreased in intraperitoneally injected or topically applicated group after irradiation with RG at 3rd and 6th weeks after irradiation. The present study suggests the RG as inhibitor of UVB-induced pigmentation and depigmenting agent.

• 농업과학연구
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• 제41권4호
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• pp.275-281
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• 2014

The ozone depletion has caused plants to be exposed to an increased penetration of solar ultraviolet-B (UV-B) radiation. Enhanced UV-B radiation may have influence on biological functions of plant in many aspects including inhibition of photosynthesis. It is evident that UV-B can potentially impair the performance of all three main component processes of photosynthesis, the photophosphorylation reactions of the thylakoid membrane, the $CO_2$-fixation reactions of the Calvin cycle and stomatal control of $CO_2$ supply. Owing to these depressed reactions, the production and allocation of carbohydrates might be markedly affected, and therefore, the growth and development of plant are distinctly reduced. In this review paper, we provide basic theory and further researches in terms of photosynthesis and carbohydrate synthesis in response to elevated UV-B radiation.

• International Journal of Industrial Entomology and Biomaterials
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• 제47권2호
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• pp.90-98
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• 2023

The human eye, constantly exposed to solar radiation, can be damaged by UV radiation. In particular, ultraviolet B (UVB)-induced damage plays an important role in retinal degeneration and cell aging. In this study, we investigated the protective effects of the methanol extract of Oxya chinensis sinuosa (OCM), an edible insect known for its high protein content (64.2%), and various pharmacological effects, on human retinal pigment epithelial cells. ARPE-19 cells were treated with OCM and subsequently UVB irradiated. Our results showed that OCM effectively attenuates UVB-induced cell damage by reducing MAPK phosphorylation (JNK and p38 MAPK). Additionally, OCM increased the phosphorylation of Akt, and cell cycle regulators, including p21 and p27, in a dose-dependent manner. Moreover, OCM treatment increased ARPE-19 cell proliferation by activating the S6K1/S6 pathway. This study suggests that OCM prevents UVB-induced retinal cell damage by increasing cell proliferation via ROS reduction, suggesting its potential as a functional therapeutic superfood against retinal cell damage.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2006년도 추계 학술발표회 발표논문집
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• pp.455-457
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• 2006

The effects of UV-B irradiation on the growth and photosynthetic activity were investigated in seedlings of sunflower(Helianthus annuus L.). The first leaves irradiated with UV-B were retarded in growth but simultaneously acquired a remarkably decreased chlorophyll fluorescence ratio compared with the non-irradiated leaves. The Fv/Fm ratio decreased by 13 % compared to the control after 2 d, and was kept to be lower than the control level until 5 d. From the results it is suggested that UV-B radiation may induce reduced biomass production and decrease in photosynthetic rate in sunflower plants.

• Journal of Radiation Protection and Research
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• 제32권2호
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• pp.59-64
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• 2007

C57BL/6 마우스에서 자외선 B(UVB)조사에 의한 표피 멜라닌세포의 변화에 대한 대나무(분죽, Phyllostachys nigra var. henenis Suapf ) 잎 추출물 (BLE)의 효과를 관찰하였다. 마우스에 UVB를 매일 $80mJ/cm^2(0.5mW/sec)$씩 7일간 조사하고 BLE를 UV조사 전 또는 조사 후에 복강내주사 또는 피부에 도포하여 멜라닌세포 형성 억제효과 및 형성된 멜라닌세포에 대한 미백효과를 dihydroxyphenylalanine (DOPA) 염색으로 관찰하였다. 마우스의 귀등쪽 표피를 분리하여 관찰한바, 정상대조군에서는 $mm^2$당 11-16개의 멜라닌세포가 관찰되었으며, UV조사 일주일 후 발달된 가지를 가진 DOPA 양성 멜라닌세포는 급격히 증가하였다. 멜라닌세포 형성 억제 실험에서는 평균치를 기준으로 복강내 주사군에서 29.9%, 피부도포군에서 33.3%의 유의성 있는 억제 효과가 관찰되었으며, 형성된 멜라닌세포의 감소 효과 실험에서는 평균치를 기준으로 복강내 주사군의 경우 6주에 24.4%의 감소 효과가 관찰되었고, 피부도포군의 경우 3주에 21.0%, 6주에 10.4%의 유의성 있는 감소 효과를 보였다. 이상의 결과는 BLE가 UV에 의한 멜라닌세포 형성 억제제 및 미백제로서의 적용 가능성을 제시하였다.