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The Effect of Bamboo (Phyllostachys nigra var. henenis Strapf) Leaf Extract on Epidermal Melanocytes in Ultraviolet B-irradiated Mice  

Lee, Hae-June (College of Veterinary Medicine, Chonnam National University)
Chae, Se-Lim (College of Veterinary Medicine, Chonnam National University)
Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University)
Publication Information
Journal of Radiation Protection and Research / v.32, no.2, 2007 , pp. 59-64 More about this Journal
Abstract
We induced the activation of melanocytes in the epidermis of C57BL/6 mice by ultraviolet B (UVB) irradiation and observed the effect of bamboo (Phyllostachys nigra var. henenis Strapf) leaf extract (BLE) on the formation, and decrease of UVB-induced epidermal melanocytes. C57BL/6 mice were irradiated by $UVB\;80mJ/cm^2(0.5mW/sec)$ daily for 7 days, and BLE was intraperitoneally or topically applied pre-or post-irradiation. For the estimation of change of epidermal melanocytes, light microscopic observation with dihydroxyphenylalanine (DOPA) stain was performed. Split epidermal sheets prepared from the ear of untreated mice exhibited 11-16 $melanocytes/mm^2$, and one week after UV irradiation, the applied areas show an increased number of strongly DOPA-positive melanocytes with stout dendrites. But intraperitoneal or topical treatment with BLE before each irradiation interrupted UVB-induced pigmentation and resulted in a marked reduction in the number of epidermal melanocytes as compared to radiation control skin. The number and size of DOPA-positive epidermal melanocytes were also significantly decreased in intraperitoneally injected or topically applicated group after irradiation with BLE at 3rd and 6th weeks after irradiation. The results of present study indicate that BLE is likely to be useful as inhibitor of UVB-induced pigmentation and depigmenting agent.
Keywords
Ultraviolet B; Melanocyte; Baboo leaf extract;
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1 Tomita K, Oda N, Ohbayashi M, Kamei H, Miyaki T, Oki T. A new screening method for melanin biosynthesis inhibitors using Streptomyces bikiniensis. J Antibiot (Tokyo) 1990;43: 1601-1605   DOI   PUBMED
2 Chakraborty AK, Funasaka Y, Komoto M, Ichihashi M. Effect of arbutin on melanogenic proteins in human melanocytes. Pigment Cell Res. 1998;11:206-212   DOI   ScienceOn
3 김정아, 최지영, 손애랑, 박성희, 허광화, 이종구, 오인석, 김진준, 장현욱, 정시령, 장태수, 이승호. 대극과 식물로부터 분리한 천연폴리페놀의 멜라닌 생성 억제효과. 생약학회지 2004;35:157-163
4 천현자, 황상구, 이진선, 백승화, 전병훈, 우원홍. 소목의 부탄 올 추출물에 의한 Melan-a 세포의 멜라닌생성 억제효과. 생약 학회지 2002;33:130-136
5 Slominski A, Tobin DJ, Shibahara S, Wortsman J. Melanin pigmentation in mammalian skin and its hormonal regulation. Physiol Rev. 2004;84:1155-1228   DOI   ScienceOn
6 Gerson DE, Szabo G. Effect of single gene substitution on the melanocyte system of the C57BL mouse: quantitative and qualitative histology. Nature 1968;218:381-382   DOI   ScienceOn
7 Hu C, Zhang Y, Kitts DD. Evaluation of antioxidant and prooxidant activities of bamboo Phyllostachys nigra var. Henonis leaf extract in vitro. J Agric Food Chem. 2000 Aug;48(8):3170-3176   DOI   ScienceOn
8 Tang LL, Ding XL. Extraction of bamboo amylase and its biological functions. Dev Res Food. 2000;21(1):8-10
9 Ma W, Wlaschek M, Tantcheva-Poor I, Schneider LA, Naderi L, Razi-Wolf Z, Schuller J, Scharffetter-Kochanek K. Chronological ageing and photoageing of the fibroblasts and the dermal connective tissue. Clin Exp Dermatol. 2001 Oct;26(7):592-599   DOI   ScienceOn
10 Hearing VJ. Biogenesis of pigment granules: a sensitive way to regulate melanocyte function. J Dermatol Sci. 2005 Jan;37(1):3-14   DOI   ScienceOn
11 Maeda K, Fukada M. In vivo effectiveness of several whitening cosmetic components in human melanocytes. J Soc Cosmet Chem. 1991;42:361-368
12 Kreiner PW, Gold CJ, Keirns JJ, Brock WA, Bitensky MW. Hormonal control of melanocytes: MSH-sensitive adenyl cyclase in the Cloudman melanoma. Yale J Biol Med. 1973;46:583-591   PUBMED
13 Giacomini P, Imberti L, Aguzzi A, Fisher PB, Trinchieri G, Ferrone S. Immunochemical analysis of the modulation of human melanoma-associated antigens by DNA recombinant immune interferon. J Immunol. 1985;135:2887-2894   PUBMED
14 Liu XY, Ding J. Study on the extraction and stability of bamboo leaf's chlorophyll, Chem Res Appl. 2000;12(2):202- 204
15 Fu XC, Wang MW, Li SP, Zhou Q, Li YQ. Effect of bamboo leaf extracts on cardiac function and hemodynamic anesthetized dogs. Chin Traditi Herbal Drugs. 2004;35(s):141-144
16 Shibata M, Yamatake Y, Sakamoto M, Kanamori M, Takagi K. Phamacological studies on bamboo grass (1). Acute toxicity and anti-inflammatory and antiulcerogenic activities of water-soluble fraction (Folin) extracted from Sasa albomarginata Makino et Shibata. Nippon Yakurigaku Zasshi. 1975 Jul;71(5):481-490   DOI   ScienceOn
17 Tang LL, Xu RR, Ding XL. The inhibitory effect of bamboo leaf polysaccharide on implanted Sarcoma l80 tumor. J Wuxi Univ Light Ind. 1998;17:62-65   과학기술학회마을
18 Zhang Y, Ding XL. Studies on anti-oxidative fraction in bamboo leaves and its capacity to scavenge active oxygen radicals. J Bamboo Res. 1996;15(3):17-24
19 Hearing VJ, Jimenez M. Mammalian tyrosinase-the critical regulatory control point in melanocyte pigmentation. Int J Biochem. 1987;19:1141-1147   DOI   ScienceOn
20 이충환, 고영희. 멜라닌 생합성 저해물질의 탐색. 생물산업 1996;9:32-35
21 Shin NH, Ryu SY, Choi EJ, Kang SH, Chang IM, Min KR, Kim Y. Oxyresveratrol as the potent inhibitor on dopa oxidase activity of mushroom tyrosinase. Biochem Biophys Res Commun. 1998;243:801-803   DOI   ScienceOn
22 Kahn V. Effect of kojic acid on the oxidation of DL-DOPA, norepinephrine, and dopamine by mushroom tyrosinase. Pigment Cell Res. 1995;8:234-240   DOI   ScienceOn
23 Laskin JD, Piccinini LA. Tyrosinase isozyme heterogeneity in differentiating B16/C3 melanoma. J Biol Chem. 1986;261:16626-16635   PUBMED
24 Reynolds J. The epidermal melanocytes of mice. J. Anat. 1954;88:45-58   PUBMED
25 Kweon MH, Hwang HJ, Sung HC. Identification and antioxidant activity of novel chlorogenic acid derivativesfrom bamboo (Phyllostachys edulis).J Agric Food Chem. 2001 Oct;49(10):4646-4655   DOI   ScienceOn
26 Kameyama K, Takemura T, Hamada Y, Sakai C, Kondoh S, Nishiyama S, Urabe K, Hearing VJ. Pigment production in murine melanoma cells is regulated by tyrosinase, tyrosinaserelated protein 1 (TRP1), DOPAchrome tautomerase (TRP2), and a melanogenic inhibitor. J Invest Dermatol. 1993;100:126-131   DOI   ScienceOn
27 Tomita Y, Fukushima M, Tagami H. Stimulation of melanogenesis by cholecalciferol in cultured human melanocytes : a possible mechanism underlying pigmentation after ultraviolet irradiation. Tohoku J Exp Med. 1986;149:451-452   DOI
28 Xu G, Zhang H. A comparison of the antimicrobial function of both extracts to bacterium between bamboo leaves and Artemisia anomala s. moore. Food Sci Technol. 2001;6:38- 39
29 Yamakoshi J, Otsuka F, Sano A, Tokutake S, Saito M, Kikuchi M, Kubota Y. Lightening effect on ultraviolet-induced pigmentation of guinea pig skin byoral administration of a proanthocyanidin-rich extract from grape seeds. Pigment Cell Res. 2003 Dec;16(6):629-638   DOI   ScienceOn
30 김영근, 박윤기, 김홍직. 자외선B의 조사가 C57BL마우스 표 피의 멜라닌세포에 미치는 영향. 대한피부과학회지 1988;26(2): 139-144
31 Zhang Y, Wu XQ, Yu ZY. Activity of the leaves of bamboo, phyllostachys nigra, and ginkgo bilabo. Chin J Chin Meteria Medica. 2002;27(4):254-257
32 Weixiong L, Helene ZH. Induced melanin reduces mutations and killing in mouse melanoma. Phytochem Phytobiol. 1997;65:480-484   DOI   ScienceOn
33 Naeyaert JM, Eller M, Gordon PR, Park HY, Gilchrest BA. Pigment content of cultured human melanocytes does not correlate with tyrosinase message level. Br J Dermatol. 1991;125:297-303   DOI   ScienceOn
34 김청택, 김원찬, 진무현, 김호정, 강상진, 강세훈, 정민환, 임영희. 전호의 멜라닌 생성억제 물질. 생약학회지 2002;33:395-398
35 Dawley RM, Flurkey WH. 4-hexylresocinol, a potent inhibitor of mushroom tyrosinase. J Food Sci. 1993;58:609-610   DOI   ScienceOn
36 과학백과사전출판사. 약초의 성분과 이용. 서울, 일월서각, 1991:653-654
37 최두영, 안소영, 이승기, 한정선, 김은철, 이향복, 신정현, 김은기, 노경호. 홍경천에 포함된 미백성분의 분리 및 성능검사. 한국생물공학회지 2004;19:169-173
38 Nerya O, Vaya J, Musa R, Izrael S, Ben-Arie R, Tamir S. Glabrene and isoliquiritigenin as tyrosinase inhibitors from licorice roots. J Agric Food Chem. 2003;51:1201-1207   DOI   ScienceOn
39 Huang W, Wang Y, Hu XB, Yin JT. Study on antimicrobial characteristics of bamboo leaf extracts. Chem Ind For Prod. 2002;22:68-70
40 Zhou ZX. The studies on the chemical constituents of bamboo leaves. Res Dev Nat Prod. 1992;4(1):44-51
41 김유찬, 윤재일. 자외선을 조사한 C57BL mice에서 자외선 조 사부 및 차단부 표피내 멜라닌 세포의 변화에 관한 연구. 대한 피부과학회지 1988;26:283-291
42 Zhang Y, Shen JF, Yu ZY, Lu BY, Lou DD. Primary studies on bamboo leaf flavonoids used as anti-aging factor for skin protection. Chem Ind For Prod. 2004;24:95-100
43 Ando S, Ando O, Suemoto Y, Mishima Y. Tyrosinase gene transcription and its control by melanogenic inhibitors. J Invest Dermatol. 1993;100:150S-155S   DOI
44 Kaufman RJ. Vectors used for expression in mammalian cells. Meth In Enzymol. 1991;205:87-92
45 Aroca P, Urabe K, Kobayashi T, Tsukamoto K, Hearing VJ. Melanin biosynthesis patterns following hormonal stimulation. J Biol Chem. 1993;268:25650-25655
46 Jimbow K, Uesugi T. New melanogenesis and photobiological processes in activation and proliferation of precursor melanocytes after UV-exposure: ultrastructural differentiation of precursor melanocytes from Langerhans cells. J Invest Dermatol. 1982;78:108-115   DOI   ScienceOn