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Screening of Crude Drugs for the Inhibitory Effect on $NF-{\kappa}B$ Activation in Transfectant HaCaT Cells  

Ahn, Kwang-Seok (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Kim, Seong-Kie (Korea Research Institute of Chemical Technology)
Moon, Ki-Young (Department of Clinical Pathology, Kwangju Health College)
Hahn, Bum-Soo (Metabolic Engineering Division, National Institute of Agricultural Biotechnology)
Kang, Sam-Sik (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Kim, Yeong-Shik (Natural Products Research Institute, College of Pharmacy, Seoul National University)
Publication Information
Korean Journal of Pharmacognosy / v.34, no.2, 2003 , pp. 156-160 More about this Journal
Abstract
$NF-{\kappa}B$ (nuclear factor-kappa B) plays a particularly central role in epidermal biology. It has been established that ultraviolet radiation (UVR) is one of the mechanisms to induce the activation of $NF-{\kappa}B$ in human skin. We previously demonstrated that melanogenic inhibitors may act through the inhibition of $NF-{\kappa}B$ activation in keratinocytes. In order to find another type of melanogenic inhibitors of $NF-{\kappa}B$ activation, various kinds of the extracts from crude drugs $(30\;{\mu}g/ml)$ were preincubated with transfectant HaCaT cells for 3 hrs and then UVR $(60\;mj/cm^2)$ was irradiated. UVR-exposed cells were incubated for another 6 hrs to measure the $NF-{\kappa}B$ activity. $NF-{\kappa}B$ activation was measured with the secreatory alkaline phosphates (SEAP) reporter gene assay using a fluorescence detection method. Among natural products, Lycium chinense, Acanthopanax senticosus, Angelica koreana, Kalopanax pictus and Asparagus cochinchinensis were the most potent inhibitors of $NF-{\kappa}B$ activation by UVR. These observations suggest that some crude drugs might act partially through the modulation of the synthesis of melanotrophic factors to decrease melanogenesis in keratinocytes.
Keywords
Transfectant HaCaT Cells; Ultraviolet radiation; Nuclear factor-kappa B activity; Melanotrophic factors; Melanogenesis;
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