• 한국식품영양과학회지
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• 제33권5호
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• pp.785-790
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• 2004

배추김치, 갓김치, 부추김치의 용매획분 추출물을 제조한 뒤 항산화관련 물질들의 함량을 측정한 결과 카로테노이드는 갓김치의 hexane층에서, 클로로필 함량은 부추김치의 hexane층에서 가장 높았고, 비타민 C함량은 갓김치의 EtOAc층과 BuOH층에서 가장 높게 나타났다. 총 폐놀화합물은 부추김 치의 CH$_2$Cl$_2$층과 EtOAc층, 배추김치 CH$_2$Cl$_2$층, 갖김치 EtOAc층과 CH$_2$Cl$_2$층에 많이 함유되어 있었다. DPPH법을 이용한 유리기 소거능을 조사한 결과 부추김치 CH$_2$Cl$_2$층과 EtOAc층, 배추김치 CH$_2$Cl$_2$층, 갓김치 EtOAc층에서 효과가 큰 것으로 나타났다. 김치종류별 용매획분을 hairless mouse의 피부균질액에 첨가하여 자외선 B를 조사한 후 지질과산 화물 함량을 측정한 결과 부추김치의 CH$_2$Cl$_2$층과 EtOAc층, 배추김치, 갓김치의 EtOAc층에서 낮게 나타나 항산화성이 높은 획분에서 피부의 광산화 억제효과가 큰 것을 알 수 있었고 김치 용매획분을 크림에 첨가하여 hairless mouse의 등에 직접 도포한 후 자외선 B를 조사하였을 때 대조군에 비해 갓김치의 hexane층, 부추김치의 hexane층과 BuOH층을 제외한 모든 용매획분이 피부홍반의 생성을 억제하였고 특히 갖김치 EtOAc층, 배추김치 CH$_2$Cl$_2$층, 부추김치 물층에서 피부보호효과가 큰 것으로 나타났다.

• 한국동물생명공학회지
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• 제34권1호
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• pp.20-29
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• 2019

Soy isoflavones have been reported to possess many physiological activities such as antioxidant activity and inhibition of cancer cell proliferation. This study investigated the photoprotective effects of soybean extract in human fibroblast cell line and hairless mice model. Human fibroblast was treated with soybean extract before and after ultraviolet B (UVB; 290-302 nm) irradiation. In the soybean extract treated group, the cells showed better resistance to ultraviolet (UV) than control group. The amount of type I collagen recovered from the soybean treated group was higher than the vehicle group exposed to UV-induced damage. Moreover, increased expression of metalloproteinases-1 as a result of UV irradiation was suppressed by the soybean extract. Female mice were orally administered soybean extract and irradiated with UVB light for 8 weeks. The effects of the soybean extract on the skin appearance, collagen deposition and epidermal thickness in the UV-damaged mouse skin were analyzed using histopathological methods. In soybean extract treated group, the skin had a better morphology than that of the control group. Furthermore, the amount of type I collagen was increased and overexpression of MMP-1 was reduced in the soybean extract group compared to vehicle group. Additionally, up-regulation of pro-inflammatory cytokines induced by UV irradiation was suppressed by dietary soybean extract treatment. It appears that soybean extract had a photoprotective effect, including anti-aging and anti-inflammatory effect, from UV-induced damage in not only human fibroblast, but also hairless mice. We confirmed that these effects were possibly due to promotion of collagen synthesis and inhibition of MMP-1 expression.

• Journal of Microbiology and Biotechnology
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• 제24권12호
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• pp.1736-1743
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• 2014

In this study, we evaluated the effect of Lactobacillus plantarum HY7714 on skin hydration in human dermal fibroblasts and in hairless mice. In Hs68 cells, L. plantarum HY7714 not only increased the serine palmitoyltransferase (SPT) mRNA level, but also decreased the ceramidase mRNA level. In order to confirm the hydrating effects of L. plantarum HY7714 in vivo, we orally administered vehicle or L. plantarum HY7714 at a dose of $1{\times}10^9CFU/day$ to hairless mice for 8 weeks. In hairless mice, L. plantarum HY7714 decreased UVB-induced epidermal thickness. In addition, we found that L. plantarum HY7714 administration suppressed the increase in transepidermal water loss and decrease in skin hydration, which reflects barrier function fluctuations following UV irradiation. In particular, L. plantarum HY7714 administration increased the ceramide level compared with that in the UVB group. In the experiment on SPT and ceramidase mRNA expressions, L. plantarum HY7714 administration improved the reduction in SPT mRNA levels and suppressed the increase in ceramidase mRNA levels caused by UVB in the hairless mice skins. Collectively, these results suggest that L. plantarum HY7714 can be a potential candidate for preserving skin hydration levels against UV irradiation.

• Journal of Microbiology and Biotechnology
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• 제28권8호
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• pp.1391-1400
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• 2018

Angelica tenuissima root has historically been used as a traditional medicine in Korea. Previous studies have identified the anti-melanogenic effects of the extract of A. tenuissima root fermented by Aspergillus oryzae (FAT). This study investigated the protective effects of FAT against ultraviolet light B exposure (UVB; $30mJ/cm^2$) in HaCaT (human keratinocyte) or Hs68 (human foreskin fibroblast) skin cells. FAT treatment was able to stimulate wound healing rate at the basal condition. FAT also favored the maintenance and/or improvement of extracellular matrix impairment caused by UVB irradiation through: 1) upregulation of procollagen Type-1 synthesis and secretion; 2) suppression of MMP-1 and elastase expression. FAT was able to play a role in the attenuation of inflammatory responses caused by UVB irradiation via upregulation of photo-protective hemeoxygease-1 and suppression of proinflammatory cyclooxygenase-2 expression. After further verification of the anti-photoaging potential of FAT, it could be utilized as an effective ingredient in anti-aging and anti-wrinkle cosmetics.

• Biomolecules & Therapeutics
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• 제23권6호
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• pp.557-563
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• 2015

Skin aging is the most readily observable process involved in human aging. Ultraviolet B (UVB) radiation causes photo-oxidation via generation of reactive oxygen species (ROS), thereby damaging the nucleus and cytoplasm of skin cells and ultimately leading to cell death. Recent studies have shown that high levels of solar UVB irradiation induce the synthesis of matrix metalloproteinases (MMPs) in skin fibroblasts, causing photo-aging and tumor progression. The MMP family is involved in the breakdown of extracellular matrix in normal physiological processes such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes such as arthritis and metastasis. We investigated the effect of diphlorethohydroxycarmalol (DPHC) against damage induced by UVB radiation in human skin keratinocytes. In UVB-irradiated cells, DPHC significantly reduced expression of MMP mRNA and protein, as well as activation of MMPs. Furthermore, DPHC reduced phosphorylation of ERK and JNK, which act upstream of c-Fos and c-Jun, respectively; consequently, DPHC inhibited the expression of c-Fos and c-Jun, which are key components of activator protein-1 (AP-1, up-regulator of MMPs). Additionally, DPHC abolished the DNA-binding activity of AP-1, and thereby prevented AP-1-mediated transcriptional activation. These data demonstrate that by inactivating ERK and JNK, DPHC inhibits induction of MMPs triggered by UVB radiation.

• Biomolecules & Therapeutics
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• 제27권4호
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• pp.395-403
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• 2019

Purpurogallin, a natural phenol obtained from oak nutgalls, has been shown to possess antioxidant, anticancer, and anti-inflammatory effects. Recently, in addition to ultraviolet B (UVB) radiation that induces cell apoptosis via oxidative stress, particulate matter 2.5 ($PM_{2.5}$) was shown to trigger excessive production of reactive oxygen species. In this study, we observed that UVB radiation and $PM_{2.5}$ severely damaged human HaCaT keratinocytes, disrupting cellular DNA, lipids, and proteins and causing mitochondrial depolarization. Purpurogallin protected HaCaT cells from apoptosis induced by UVB radiation and/or $PM_{2.5}$. Furthermore, purpurogallin effectively modulates the pro-apoptotic and anti-apoptotic proteins under UVB irradiation via caspase signaling pathways. Additionally, purpurogallin reduced apoptosis via MAPK signaling pathways, as demonstrated using MAPK-p38, ERK, and JNK inhibitors. These results indicate that purpurogallin possesses antioxidant effects and protects cells from damage and apoptosis induced by UVB radiation and $PM_{2.5}$.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2006년도 추계 학술발표회 발표논문집
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• pp.455-457
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• 2006

The effects of UV-B irradiation on the growth and photosynthetic activity were investigated in seedlings of sunflower(Helianthus annuus L.). The first leaves irradiated with UV-B were retarded in growth but simultaneously acquired a remarkably decreased chlorophyll fluorescence ratio compared with the non-irradiated leaves. The Fv/Fm ratio decreased by 13 % compared to the control after 2 d, and was kept to be lower than the control level until 5 d. From the results it is suggested that UV-B radiation may induce reduced biomass production and decrease in photosynthetic rate in sunflower plants.

• Nutrition Research and Practice
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• 제8권4호
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• pp.398-403
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• 2014

BACKGROUND/OBJECTIVES: Ultraviolet B (UVB) irradiation on skin can induce production of reactive oxygen species (ROS), which cause expression of matrix metalloproteinases (MMPs) and collagen degradation. Thus, chronic exposure of skin to UVB irradiation leads to histological changes consistent with aging, such as wrinkling, abnormal pigmentation, and loss of elasticity. We investigated the protective effect of the standardized green tea seed extract (GSE) on UVB-induced skin photoaging in hairless mice. MATERIALS/METHODS: Skin photoaging was induced by UVB irradiation on the back of Skh-1 hairless mice three times per week and UVB irradiation was performed for 10 weeks. Mice were divided into six groups; normal control, UVB irradiated control group, positive control (UVB + dietary supplement of vitamin C 100 mg/kg), GSE 10 mg/kg (UVB + dietary supplement of GSE 10 mg/kg), GSE 100 mg/kg (UVB + dietary supplement of GSE 100 mg/kg), and GSE 200 mg/kg (UVB + dietary supplement of GSE 200 mg/kg). RESULTS: The dietary supplement GSE attenuated UVB irradiation-induced wrinkle formation and the decrease in density of dermal collagen fiber. In addition, results of the antioxidant analysis showed that GSE induced a significant increase in antioxidant enzyme activity compared with the UVB irradiation control group. Dietary supplementation with GSE 200 mg/kg resulted in a significant decrease in expression of MMP-1, MMP-3, and MMP-9 and an increase in expression of TIMP and type-1 collagen. CONCLUSIONS: Findings of this study suggest that dietary supplement GSE could be useful in attenuation of UVB irradiation-induced skin photoaging and wrinkle formation due to regulation of antioxidant defense systems and MMPs expression.

• Korean Chemical Engineering Research
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• 제56권4호
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• pp.524-535
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• 2018

본 연구에서는 에멀젼 자기조립틀을 활용하여 거대기공을 갖는 타이타니아 분말을 합성하였으며, 자외선 조사를 활용한 광촉매 실험을 통해 상용화된 타이타니아 나노입자와 다공질 타이타니아 분말을 이용한 고초균의 살균 효과를 비교하였다. 다공질 분말의 기공 크기를 달리하여 자외선 조사 시간에 따른 고초균 수의 감소를 측정하였으며, 살균 실험의 최적 조건을 도출하기 위하여 기공 크기에 따른 다공질 타이타니아 분말의 살균력을 비교하였다. 기공 크기가 작아질수록 살균 효과는 증대됨을 관찰하였으며, 광촉매의 활용 및 자외선 조사에 의해 1시간 동안 50% 이상의 고초균이 제거되는 효과를 확인하였다. 또한 활성 화학종의 발생을 촉진하기 위하여 과산화수소의 희석 용액을 광촉매 살균 방식과 결합하였으며, 1시간 동안의 자외선 조사에 의해 균주의 거의 대부분이 사멸되는 등 고초균에 대한 살균 효과를 증진시킬 수 있었다.

• 생명과학회지
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• 제20권1호
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• pp.27-32
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• 2010

자외선 B는 반응성 산소종(reactive oxygen species, ROS)의 발생을 유도하고 세포내 항산화 물질의 저장을 방해한다. 후코이단은 L-fucose와 황 에스터기를 포함하는 다당류로서 갈조류의 주요 성분으로 알려져 있다. 본 연구에서는 인간 피부 섬유아세포인 HS68세포에 자외선을 조사하여 산화 스트레스를 유도한 후 후코이단의 보호효과를 확인하고자 하였다. 후코이단의 전처치로 malondialdehyde (MDA)의 양이 용량-의존적으로 감소하였고, 세포내 glutathiopne도 후코이단 $100\;{\mu}g/ml$을 투여하였을 때 후코이단을 투여하지 않고 자외선만 조사한 군에 비하여 21.5% 감소하였다. 후코이단을 10, $100\;{\mu}g/ml$의 용량으로 투여하였을 경우 자외선 B에 의한 ROS 생성이 자외선만 조사하였을 때 보다 각각 40.1%와 68.4%로 유의하게 감소하였다. 세포노화와 관련된 $\beta$-galactosidase의 양성 염색률은 후코이단을 10, $100\;{\mu}g/ml$ 투여하였을 경우 자외선만 조사하였을 때보다 각각 23.1%, 16.4% 감소하였다. 이러한 결과로 미루어 후코이단은 자외선에 의한 산화 스트레스에 대하여 광보호 효과가 있는 것으로 생각된다.