Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Diphlorethohydroxycarmalol Suppresses Ultraviolet B-Induced Matrix Metalloproteinases via Inhibition of JNK and ERK Signaling in Human Keratinocytes

  • Piao, Mei Jing (School of Medicine, College of Natural Sciences, Jeju National University) ;
  • Kumara, Madduma Hewage Susara Ruwan (School of Medicine, College of Natural Sciences, Jeju National University) ;
  • Kim, Ki Cheon (School of Medicine, College of Natural Sciences, Jeju National University) ;
  • Kang, Kyoung Ah (School of Medicine, College of Natural Sciences, Jeju National University) ;
  • Kang, Hee Kyoung (School of Medicine, College of Natural Sciences, Jeju National University) ;
  • Lee, Nam Ho (Department of Chemistry, College of Natural Sciences, Jeju National University) ;
  • Hyun, Jin Won (School of Medicine, College of Natural Sciences, Jeju National University)
  • Received : 2015.05.11
  • Accepted : 2015.09.08
  • Published : 2015.11.01
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Abstract

Skin aging is the most readily observable process involved in human aging. Ultraviolet B (UVB) radiation causes photo-oxidation via generation of reactive oxygen species (ROS), thereby damaging the nucleus and cytoplasm of skin cells and ultimately leading to cell death. Recent studies have shown that high levels of solar UVB irradiation induce the synthesis of matrix metalloproteinases (MMPs) in skin fibroblasts, causing photo-aging and tumor progression. The MMP family is involved in the breakdown of extracellular matrix in normal physiological processes such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes such as arthritis and metastasis. We investigated the effect of diphlorethohydroxycarmalol (DPHC) against damage induced by UVB radiation in human skin keratinocytes. In UVB-irradiated cells, DPHC significantly reduced expression of MMP mRNA and protein, as well as activation of MMPs. Furthermore, DPHC reduced phosphorylation of ERK and JNK, which act upstream of c-Fos and c-Jun, respectively; consequently, DPHC inhibited the expression of c-Fos and c-Jun, which are key components of activator protein-1 (AP-1, up-regulator of MMPs). Additionally, DPHC abolished the DNA-binding activity of AP-1, and thereby prevented AP-1-mediated transcriptional activation. These data demonstrate that by inactivating ERK and JNK, DPHC inhibits induction of MMPs triggered by UVB radiation.

Keywords

References

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