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Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5  

Zhen, Ao Xuan (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine)
Piao, Mei Jing (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine)
Hyun, Yu Jae (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine)
Kang, Kyoung Ah (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine)
Ryu, Yea Seong (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine)
Cho, Suk Ju (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine)
Kang, Hee Kyoung (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine)
Koh, Young Sang (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine)
Ahn, Mee Jung (Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University)
Kim, Tae Hoon (Department of Food Science and Biotechnology, Daegu University)
Hyun, Jin Won (Jeju National University School of Medicine and Jeju Research Center for Natural Medicine)
Publication Information
Biomolecules & Therapeutics / v.27, no.4, 2019 , pp. 395-403 More about this Journal
Purpurogallin, a natural phenol obtained from oak nutgalls, has been shown to possess antioxidant, anticancer, and anti-inflammatory effects. Recently, in addition to ultraviolet B (UVB) radiation that induces cell apoptosis via oxidative stress, particulate matter 2.5 ($PM_{2.5}$) was shown to trigger excessive production of reactive oxygen species. In this study, we observed that UVB radiation and $PM_{2.5}$ severely damaged human HaCaT keratinocytes, disrupting cellular DNA, lipids, and proteins and causing mitochondrial depolarization. Purpurogallin protected HaCaT cells from apoptosis induced by UVB radiation and/or $PM_{2.5}$. Furthermore, purpurogallin effectively modulates the pro-apoptotic and anti-apoptotic proteins under UVB irradiation via caspase signaling pathways. Additionally, purpurogallin reduced apoptosis via MAPK signaling pathways, as demonstrated using MAPK-p38, ERK, and JNK inhibitors. These results indicate that purpurogallin possesses antioxidant effects and protects cells from damage and apoptosis induced by UVB radiation and $PM_{2.5}$.
Purpurogallin; Ultraviolet B radiation; Particulate matter 2.5; Oxidative stress; Human HaCaT keratinocytes;
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