• Applied Microscopy
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• 제24권3호
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• pp.34-45
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• 1994

This investigation has been carried out to examine the structure of digestive tract from Korean Leech, Erpobdella lineata, using light and electron microscope. The digestive tract is composed of mouth, pharynx, Oesophagus, six-chambered stomach, three-chambered intestine, rectum and anus. Stomach and intestine have not gastric or intestine ceca and consist of only straight tube. All digestive tracts from pharynx to rectum are covered with simple columnar epithelial cells. While the surfaces of endothelial cell of pharynx and rectum are covered with cuticular layer of about $0.3{\mu}m$ in thickness, stomach and intestine are covered with estimated $0.2-0.3{\mu}m$ and $0.5{\mu}m$ microvilli respectively. Circular folds were found only in first and second chambers of stomach, intestine and rectum, but not in pharynx and the other chambers (third to sixth) of stomach. The granules of $0.3-0.8{\mu}m$ and $0.5-1.0{\mu}m$ in diameter were observed in the cytoplasm of stomach endothelial cell. These granules were demonstrated to contain protein which showed a positive reaction to ninhydrin. It was also found that there are well-developed microvilli in the apical portion of intestine endothelial cell in which endocytosis occurs actively.

• 한국자원식물학회지
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• 제16권1호
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• pp.61-73
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• 2003

한국산 용담과 식물중, 덩굴식물인 좁은잎덩굴용담속과 덩굴용담속에는 각기 1분류군씰 분포한다. 이에 대한 외부형태학적 형질을 재검토하였으며, 줄기, 잎, 자방, 악, 자방의 횡단면, 주두, 잎표피, 주맥표면, 화관열편 표면, 밀선, 종피와 화분의 형태를 조사하여 두 속간의 한계 와 유연관계를 재검토하고자 하였다. 두 분류군의 식별형질로는 주로 열매형질(삭과와 장과), 부화관의 유무와 화분형 질(망상과 유선상)로 의존해 왔으나, 조사결과 외부형태형질(뿌리, 잎, 꽃받침, 주두, 약)과 내 부형태형질(꽃받침, 자방) 그리고 미세구조(화피열편의 표면, 잎의표면, 주맥의 표면, 종피)에서도 커다란 차이를 보였다. 두 분류군의 분포는 한반도 육지역에 분포하는 것은 전부 좁은잎덩굴용담이었고 덩굴용담은 해양성기후대인 제주도와 울릉도에서만 발견되었다.

• 생명과학회지
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• 제12권4호
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• pp.399-406
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• 2002

본 연구에서는 DEB를 애기장대 종자에 처리하여 엽록체 발달에 관련된 돌연변이체들을 분리하였다. 이들은 각각 그 특징에 따라 iml, gev, yev로 명명하였으며 iml은 잎에 얼룩무늬를 나타내는 돌연변이체이며, gev는 엽맥에만 녹색을 나타내고 이외의 잎에서는 연한녹색을 나타내며, yev는 엽맥은 연한녹색을 나타내고 엽맥 이외의 잎에서는 녹색을 나타내었다. 주사 전자현미경을 이용하여 엽록체 상세구조를 관찰하여 본 결과 야생형 엽록체의 그라나 티라코이드의 모양은 규칙적인 배열하는 그라나가 쌓여 있었으나, iml, gev, yev 돌연변이체 에서는 그라나 티라코이드의 모양이 불규칙하며 그라나의 수도 불규칙하며 스트로마 티라코이드의 연결도 야생형과 상이함을 보였다. 그리고 이들을 유전분석한 결과 야생종과 돌연변이체의 F$_2$에서 3:1의 분리비를 나타내어, 이들 돌연변이들은 핵내에 존재하는 유전자에서 돌연변이가 일어났음을 알 수 있었고, 단일열성으로 유전됨이 밝혀졌다.

• ALGAE
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• 제22권4호
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• pp.325-331
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• 2007

Cryptoglena pigra Ehrenberg from Korea was a photosynthetic euglenoid alga, which had typical characteristics of the Euglenales. The ultrastructure examination of C. pigra revealed certain features which were distinctly photosynthetic euglenoid: one U-shaped chloroplast with thylakoid membranes; two paramylon grains appressed to both sides of the chloroplast; eyespot associated with the chloroplast but not part of it. Three flagellar roots were associated with the two basal bodies. The four-membered dorsal root arose from the dorsal body and extended anteriorly following the reservoir membrane. At the base of the reservoir the dorsal band was nucleated by the dorsal root and it ran anteriorly between the reservoir membrane and eyespot. The dorsal band was continued with the microtubules of the canal and the pellicle. The singlet dorsal microtubules at the transition level arranged into doublets by a successive linkage of the existing adjacent microtubules, and the doublets rearranged into the cytoskeletal microtubules that were continuous with four microtubules in pellicles. Finally, the sixteen ridges gave rise to the pellicular ridges. The five to six-membered ventral root extended anteriorly into a cytoplasmic pocket through the reservoir and lined a cytoplasmic pocket.

• Applied Microscopy
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• 제26권2호
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• pp.177-195
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• 1996

Fine structure of the processes of intramembranous ossification and endochondral ossification at the tip of the distal phalanx of human fetuses was studied by electron microscopy. In 50 mm fetus, intramembranous ossification of the tip of cartilaginous phalanx was first noted. The osteoblasts of the perichondral zone of tip of cartilaginous phalanx started to lay down a thick membranous bony lamella. Most of the hypertrophied chondrocytes in the marginal parts of tip of the distal phalanx remained viable after being embeded in mineralized cartilaginous septa. The tuberosity of the distal phalanx was formed by membranous bony trabeculae on the exterior of the subperiosteal cap at 80 mm fetus. At this stage endochondral ossification was first observed in distal extremity of the distal phalanx. The maority of hypertrophied chondrocytes in the center of distal extremity appeared to be disintegrating. Resorption of calcified matrix was undertaken by perivascular cells and chondroclasts. From the periosteum, zone of calcification, vascular sprouts expanded within a recently opened lacunae, and the invading osteoblasts laid down osteoid and bone. After 120 mm fetus, endochondral and subperiosteal ossification proceeded in only one direction, just proximally. These findings demonstrate that intramembranous ossification, calcification, and endochondral ossification start at tip of the distal phalanx instead of at the center of the shaft, as was the case in other long bones.

• Applied Microscopy
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• 제28권3호
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• pp.399-414
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• 1998

Glandular trichomes present on the leaf surface of Drosera capensis were examined using transmission electron microscopy. A large number of stalked glands exist on the adaxial surfaces of the leaf blade. The secretory head is composed of two layers of secretory cells, one layer of middle cells, and the inner tracheids. The secretory cells contain rough endoplasmic reticulum, mitochondria, plastids, Golgi apparatus, and vacuoles. The secretory cells show prominent cell wall ingrowth, and thick cuticle restricted on the subcuticular wall. Frequently, the cuticle has some pores, canal-like structures, showing electron -dense granules being penetrated through them. Ultrastructural localization using diaminobenzidine showed the electron-dense deposits in the vacuole. No peroxidase activity was seen in the cell wall and cytolasm. The activity of peroxidase (POX) isozymes in Drosera which isoelectric point (pI) is 3.6 and some anionic POX isozymes which pIs are laid between 3.6 and 4.6 were especially increased according to the development and the formation of glandular trichomes. Also, the activity of some POX isozymes which isoelectric points are laid between 4.6 and 5.1 were increased in the regions of leaves which has trichomes.

• Applied Microscopy
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• 제47권1호
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• pp.8-12
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• 2017

The ultrastructures of the secretory acinar granules of submandibular and parotid salivary gland were examined in the Korean striped field mouse, Apodemus agraius. The acini of the submandibular salivary gland had serous and mucous acinar cells filled with numerous secretory granules. The serous acinar granules had uniformly fine dense contents and were round typed with a definite boundary between the granules. The mucous acinar granules were relatively coarse, with moderate density, and clustered together as a result of the indistinct boundaries between the granules. The acini of the parotid salivary glands contained only serous cells filled with numerous round-typed serous acinar granules. Serous acinar granules had uniformed dense matrix and definite boundaries. The ultrastructures without substructure in a matrix of serous and mucous acinar granules in the submandibular and parotid salivary glands of A. agraius were similar to those of species of Rodentia but different from those of Soricidae in Korea with a characteristic substructure in a matrix. This ultrastructure and charateristics in secretory acinar granules provide fundamental data for molecular comparisions of genetic relationships and are one of the key methods for classifying A. agraius.

• 대한수의학회지
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• 제32권2호
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• pp.207-210
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• 1992

Enterovirus감염 돼지의 감염초기 virus의 동태를 규명하고자 초유를 섭취하지 않는 1~2일령의 돼지에 돼지 enterovirus 3형을 경구감염시킨후 간조직을 전자현미경으로 관찰하였던 바 다음과 같은 결과를 얻었다. 감염후 3일째 부검한 돼지의 간에서 간세포의 심한 공포변성이 인정되었고 간 동양혈관 내피세포 및 Kupffer세포에서 picornavirus의 특징적인 virus결정이 출현하였으며 확장된 ER은 virus 결정과 밀접하게 관련되어 있었다. 또한 동양혈관 세포와 간세포에서는 미숙한 virus모양의 입자들로 구성된 세포질내 봉입체가 관찰되기도 하였다. 이상의 소견으로 보아 돼지 enterovirus 3형은 신경친화성 뿐만 아니라 감염초기 간에 친화성이 큰 것으로 생각된다.

• Fisheries and Aquatic Sciences
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• 제8권1호
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• pp.17-26
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• 2005

The ultrastructure of germ cells during spermatogenesis and the structural changes in the epithelial cells of the seminal vesicle with testicular development in male Neptunea (Barbitonia) arthritica cumingii were investigated monthly based on electron microscopic and histologic observations. N. arthritica cumingii (Gastropod: Buccinidae) undergoes internal fertilization and possesses a modified type of spermatozoon, which is approximately 20$\mu$m long. The axoneme of the tail flagellum consists of nine peripheral pairs of microtubules and one central pair. Many spermatozoa occur in the acini of the testis in the ripe stage and are transported to the seminal vesicles in the accumulating phase. In males, the monthly gonadosomatic index began to increase in September and reached a maximum in February. Subsequently, it decreased rapidly after April. The testis of this species can be classified into four developmental stages: the active (August to September), ripe (October to July), copulation (April to July), and recovery (July to August) stages. Structural changes in the epithelial cells of the seminal vesicles of this species could be classified into three phases: (1) S-I (resting), (2) S-II (accumulating), and (3) S-III (spent) phases. The morphology and structure of the epithelial cells of the seminal vesicle differed in each phase; the cells were cuboidal, squamous, or columnar in the resting, accumulating, or spent phases, respectively.

• 한국어류학회지
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• 제18권4호
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• pp.347-354
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• 2006

참갈겨니(Zacco koreanus) 정세포 변형과정과 성숙한 정자의 미세구조를 주사 및 투과 전자 현미경으로 관찰하였다. 참갈겨니 Z. koreanus 정세포변형과정은 수중형 Type II과 유사하나 기부중심립이 핵와안에 위치해 있는 점에서 일부 차이가 있었다. 또한 두 중심립은 섬유성 물질에 의하여 상호연결되어 있는데 이와 같은 구조는 잉어류에서 공통적으로 나타나고 있다. 편모에서 관찰되는 vesicle은 잉어류의 공통된 형질로 사료되며 Charciformes와 일부 Siluriformes에서도 관찰되고 있어 이들 분류군의 계통적 유연관계를 추정할 수 있는 구조적 특징으로 사료된다.