Browse > Article
http://dx.doi.org/10.5352/JLS.2002.12.4.399

Analysis of Chloroplast Mutants of Arabidopsis Induced by Diepoxybutane  

윤용휘 (경북대학교 농과대학 농학과)
이정훈 (경북대학교 농과대학 농학과)
박해진 (경북대학교 농과대학 농학과)
강용원 (경북대학교 농과대학 농학과)
이경민 (경북대학교 농과대학 농학과)
신동현 (경북대학교 농과대학 농학과)
이인중 (경북대학교 농과대학 농학과)
김학윤 (계명대학교 환경학부)
김달웅 (경북대학교 농과대학 농학과)
Publication Information
Journal of Life Science / v.12, no.4, 2002 , pp. 399-406 More about this Journal
Abstract
The Arabidopsis mutants involved in chloroplast development were induced by seed treatment of diepoxybutane which was rarely known mutagenic compound in plant mutagenesis. Three kinds of mutants designated as iml, gev, and yev were represented by the characteristics of variegated leaves, green vein with yellow leaves, and yellow green vein with green leaves respectively. We investigated the ultrastructure of chloroplast in mutated regions using transmission electron microscopy. The ultrastructure of chroloplast in wildtype showed regularly stacked grana thylakoid and stroma thylakoid while iml, gev and yev mutants displayed different shapes of grana stacking and stroma stacking of chloroplasts. Genetic analysis of three chloroplast mutants exhibit that divergent traits were ruled by a single recessive nuclear gene.
Keywords
chloroplast mutants; dieopxybutane (DEB); mutagenesis;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Mutations in the DET1 gene affect cell-type-specific expression of light-regulated genes and chlroplast development in Arabidopsis /
[ Chory,J.;C.A.Peto ] / Proc.Natl Acad.Sci   DOI   ScienceOn
2 Isolation of a gene encoding a novel chloroplast protein by T-DNA tagging in Arabidopsis thaliana /
[ Koncz,C.;R.Mayethofer;Z.Konz-kalman;C.N.Resis;G.P.Redei;J.Schell ] / EMBO J.
3 Butadiene-induced intrastrand DNA cross-links:a possible role in delection mutagenesis /
[ Carmical J.R.;A.Kowalczyk;Y.Zou;B.Van Houten;L.V.Nechev;C.M.Harris;T.M.Harris;R.S.Lloyd ] / J.Biol.Chem   DOI   ScienceOn
4 Rapid image analysis screening procedure for identifying chloroplast number mutant in mesophyll cells of Arabidopsis thaliana /
[ Pyke,K.A.;R.Leech ] / Plant Physiology   DOI   ScienceOn
5 Linkage map of Arabidopsis thaliana /
[ Koornneef,M;E.C.Hanbart;P.Stam.F.J.Braaksma;W.J.Feenstra ] / J.Heredity
6 Bundle sheath cells and cell-specifia plastid development in Arabidopsis leaves /
[ Kinsman,E.A;K.A.Pyke ] / Development
7 The unfolding drama of flower development:recent results from gentic and molecular analyses /
[ Hong M. ] / Gene&development   DOI
8 Genetics of homology-dependent gene silencing in Arabidopsis;a role for methylation /
[ Davis,G.J;M.A.Sheikh;O.J.Ratcliffe;G.Groupland;I.J.Furner ] / Plant Journey   DOI   ScienceOn
9 Arabidopsis thaliana:A new model system for molecular genetics /
[ Kang,H.G.;H.G.Nam ] / Biochemistry News of Kor.Biochem.Soc.
10 Leaf permease1 gene of maize if required for chloroplast development /
[ Neil,P.S.;T.P.Brutnell;A.Allen;S.L.Dellaporta;T.Nelson;J.Chen ] / Plant Cell   DOI   ScienceOn
11 Diepoxybutane and diepoxyoctane interstrand cross-linking of the 5S DNA nucleosomal core particle /
[ Millard J.T.;E.E.Wilks ] / Biochemistry   DOI   ScienceOn
12 Overexpression of the Arabidopsis thaliana MinD1 gene alters choroplast size and number in transgenic tobacco plants /
[ Dinkins R.;M.S.Reddy;M.Leng;G.B.Collins ] / Planta   DOI   ScienceOn
13 Intracellular signalling:The chloroplast talks! /
[ P.Jarvis ] / Current Biology   DOI   ScienceOn
14 The arabidopsis immutans mutation affects plastid differentiation and the morphogenesis of white and green sectors in variegated plants /
[ Aluru,M.R.;H.Bae;D.Wu;S.R.Rodermel ] / Plant Physiology   DOI   ScienceOn
15 The effects of reduced amouts of liqid unsaturation on chloroplast ultrastructure and photo synthesis in a mutant of arabidopsis /
[ McCourt P;L.Kunst;J.Browse;C.R.Somerville ] / Plant Physiology   DOI   ScienceOn
16 /
[ Csaba K,;J.Shell;N.H.Chua ] / Methods on arabidopsis research
17 Pollen of a male-sterile mutant of Arabidopsis thaliana isolated from a T-DNA insertion pool is not effectively released from the anther locule /
[ Park,S.k.;W.H.Yoo;B.C.Kim;Y.H.Hwang;I.K.Chung;H.G.Nam;D.U.Kim ] / Plant Cell Physiol   DOI   ScienceOn
18 Mutagenesis of Arabidopsis thaliana by diepoxybutane and mutant analysis /
[ Yoon,Y.H;D.U.Kim ] / Korean J. Breed
19 Eukaryotic peptide deformylases.Nuclear-encoded and chloroplast-targeted enzyme in arabidopsis /
[ Dirk.L.M.A.;M.A.Williams;R.L.Houtz ] / Plant Physiology   DOI   ScienceOn
20 The yellow variegated mutant of arabidopsis is plastid autonomous and delayed in chloroplast biogensis /
[ Chen,M.;M.Jensen;S.Rodermel ] / J.Heredity   DOI   ScienceOn
21 Plastid division and developent /
[ Pyke.K.A. ] / Plant Cell   DOI   ScienceOn
22 Altered mitochondrial gene expression in maternal distorted leaf mutant of Arabidopsis induced by chloroplast mutator /
[ Sakamoate,W.;H.Kondo;M.Murata;R.Motoyoshi ] / Plant Cell   DOI   ScienceOn
23 Signal transduction mutants of Arabidopsis uncouple nuclear CAB and RBCS gene expression from chloroplast development /
[ Ronald,E.S.;M.F.M.Ausbel;J.Chory ] / Cell
24 Biochemical aspects of a genetically determined variegation in Arabidopsis /
[ Redei,G.P. ] / Genetics
25 Molecular analysis of diepoxybutane-induced mutations at the rosy locus of Drosophila melanogaster /
[ Reardon,J.T.;C.A.Liljestrand-Golden;R.L.Duesenbery;P.D.Smith ] / Genetics