• Title/Summary/Keyword: ultrasonification

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Immune Activities of Rhodiola sachalinensis A. Bor Extracts Isolated with Various Extraction Process (추출조건에 따른 참돌꽃의 면역 활성)

  • Kim, Cheol-Hee;Kwon, Min-Chul;Han, Jae-Gun;Ha, Ji-Hye;Jeong, Hyang-Suk;Choi, Geun-Pyo;Park, Uk-Yeon;Nam, Jong-Hyun;Hwang, Baik;Lee, Hyeon-Yong
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.6
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    • pp.383-389
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    • 2008
  • This study was performed to compare effect of immune activities of Rhodiola sachalinensis by various extraction process with different temperature and extraction solvents. Experiments were performed for investigate the immune activities on human B and T cell growth and secretion of their cytokines. Also, antibodies in serum were investigated in female ICR mouse by feeding the extracts of R. sachalinensis at doses of 40, 120 and 360 mg/kg orally for 15 days. The immune cell growth and secretion of cytokines (IL-6, TNF-$\alpha$) on human B and T cells were increased by adding R. sachalinensis extracts compare to the control. Also, total serum IgG levels increased by feeding R. sachalinensis extracts. It can be conclude that optimum condition for efficient extraction of R. sachalinensis as functional material is slovent extraction process using water with ultrasonification at below $100^{\circ}C$ than typical process.

Antioxidant Activity, Macamide B Content and Muscle Cell Protection of Maca (Lepidium meyenii) Extracted Using Ultrasonification-Assisted Extraction

  • Buyanbadrakh, Enkhbolor;Hong, Hyeong-Suk;Lee, Kang-Woo;Huang, Wen Yan;Oh, Jun-Hyun
    • Microbiology and Biotechnology Letters
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    • v.48 no.2
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    • pp.129-137
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    • 2020
  • This study aims to evaluate the efficacy of the Ultrasonication-Assisted (UA) extraction on the functionality of the herbaceous biennial plant maca (Lepidium meyenii). The specific objectives include comparison of the antioxidant activities among various maca extracts, determination of the macamide B content of the extracts, and in vitro evaluation of maca on cell viability and creatine kinase (CK) activity. The antioxidant activities of the water, ethanol, and UA extracts were compared by determining the total phenolic and flavonoid contents, the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, and the ferric reducing antioxidant power (FRAP) of the extracts. The macamide B content of maca extracts were analyzed by HPLC. The effects of the extracts on muscle cell viability and creatine kinase activity were also determined using C2C12 myoblasts. UA extraction significantly increased the total phenolic content (2.90 GAE ㎍/mg, p < 0.05), without affecting the flavonoid content. DPPH radical scavenging activity did not exhibit any statistical difference among the extracts. The ethanol and UA extracts exhibited significantly higher FRAP than the water extract (p < 0.05). The macamide B content of ethanol and UA extracts were 0.087 and 0.083 ㎍/mg, respectively. The water and UA extracts exhibited higher C2C12 muscle cell viability than the ethanol extract, and both extracts resulted in a significantly lower CK level than the H2O2-treated control group. This research suggests that the maca extract can protect muscle cells and serve as an antifatigue agent under oxidative stress conditions.

Effects of Alcohol Oxidation of Brassica rapa L. Extraction Process in Kang-Hwa (강화산 순무의 추출 공정에 따른 알코올 분해 효과)

  • Kim, Dae-Ho;Kim, Jung-Hwa;Kim, Cheol-Hee;Kwon, Min-Chul;Kim, Hyo-Sung;Chung, Hae-Gon;Kang, Ha-Young;Lee, Hak-Ju;Lee, Hyeon-Yong
    • Korean Journal of Medicinal Crop Science
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    • v.14 no.1
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    • pp.45-48
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    • 2006
  • Alcohol dehydrogenase (ADH) and Aldehyde dehydroganase (ALDH) activities of turnip extracts with ultrasonification at $60,\;100^{\circ}C$ temperature were investigated. Ultrasonification extracts showed a increase as compared with the normal extracts. ALDH activities were high than ADH activities. So, turnip extracts was shown efficient plants in alcohol oxidation. Appraisers liked powder extracts better than liquid extracts in sensory score.

Enhancement of Immune Activities of Ephedrae Herba and Rubi Fructus at Low Temperature Extraction (저온 추출 공정에 의한 마황과 복분자의 면역 활성 증진 효과)

  • Kim, Dae-Ho;Park, Jin-Hong;Kim, Jung-Hwa;Kim, Cheol-Hee;You, Jin-Hyun;Kwon, Min-Chul;Lee, Hyeon-Yong
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.3
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    • pp.81-86
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    • 2005
  • The immune activities of the extracts from Ephedrae Herba and Rubi Frutus extraction with ultrasonification at $60^{\circ}C$ were compared with the extracts though water extraction at $100^{\circ}C$. The growth of human T cells was increased up to $13{\times}10^4\;viable\;cells/m{\ell}$ in adding $1.0\;g/{\ell}$ of the ultrasonification extracts of R. Fructus at $60^{\circ}C$, compared to adding the extracts at $100^{\circ}C$. The secretion of $TNF-{\alpha}$ for human T cells were also increased up to $13.9{\times}10^{-4}$ pg/cell by adding extracts at $60^{\circ}C$, compared to extracts at $100^{\circ}C$. The extracts of R. Fructus at $60^{\circ}C$ increased NK cell activities up to 50% and the secretion of $NO^{-1}$ from macrophage to $31\;{\mu}M$ for ultrasonification extracts at $60^{\circ}C$.

Continuous Processing of Monodispersed Zirconia Powders (초음파 혼합 연속공정에 의한 단분산 지르코니아 분체의 합성)

  • Rhee, Jhun;Jo, Dong-Soo
    • Journal of the Korean Ceramic Society
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    • v.29 no.12
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    • pp.919-925
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    • 1992
  • Continuous synthesis process for monodispersed zirconia powders with ultrasonic wave mixing was developed. Reactant solutions were flowed through a T-tube with small diameter and then mixed in a microscale with ultrasonification. Reaction and aging were followed during the mixed solution of reactants is in plug flowing through a narrow long teflon tubing. Zr(n-OC4H9)4 in ethanol and H2O in ethanol were used as reactants. From this process monodispersed, spherical, non-agglomerated, singlet hydrated zirconia powders with 0.6 $\mu\textrm{m}$ average size were obtained. Geometrical standard deviation of the particle size distribution was less than 1.2 with ultrasonic mixing, and the geometrical standard deviation was not affected by the flow rate of the reactants.

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Effects of Kanendomycin, Kanamycin on Stearic Acid Monolayer (Kanamycin 및 Kanendomycin이 인공 stearic acid 단분자막에 미치는 영향)

  • Lee, Syng-Il;Kang, Doo-Hee
    • The Korean Journal of Physiology
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    • v.9 no.2
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    • pp.1-5
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    • 1975
  • It has been reported that kanamycin lowers osmotic fragility and mechanical fragility by ultrasonification of human red cells. In the present study, we therefore investigated effects of $Ca^{++}$, kanendomycin and kanamycin on stearic acid monolayer using a langmuir trough and found fellowing results 1) $Ca^{++}$ added to the subphase condensed stearic acid monolayer, confirming earlier reports. 2) Likewise, kanendomycin and kanamycin condensed the stearic acid monolayer, the effects being dose dependent. The possible modes of action of kanamycin and kanendomycin on biological membrane were discussed and compared with those of $Ca^{++}$ and local anesthetics

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Electrical Properties of Polyaniline according to Preparation Conditions (제조 조건에 따른 Polyaniline의 전기적 성질)

  • 김언령;김태영;이보현;김종은;서광석;배종현
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.14 no.3
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    • pp.215-222
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    • 2001
  • Polyaniline-Camphorsulfonic Acid Emeraldine Salt(PANI-CSA ES) was prepared by doping Polyaniline Ermeralidine Base(PANI EB) with DL-10-Camphorsulfonic Acid(CSA). PANI-CSA ES was solved in an organic solvent by ultrasonification for different periods of time and its surface resistivity was measured. Several PANI-CSA ES solutions solved in different organic solvents were prepared and their surface resistivities were measured. Thermal stability of film casted with PANI-CAS ES solution in m-cresol was estimated by measuring its surface conductivity and the content of this moisture and organic solvents. PANI-CSA ES was blended with different polymeric binders to improve its physical properties and the surface resistivities of several kinds of PANI-CSA ES blends were measured as a function of the content of PANI-CSA ES. PANI-CSA ES polymerized by 1-step oxidative polymerization was prepared and its surface resistivity was measured.

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C60@MWCNT: Room Temperature Encapsulation of C60 into Multiwall Carbon Nanotubes

  • Gupta, Vinay;Bahl, Om P.;Mathur, Rakesh B.
    • Carbon letters
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    • v.11 no.1
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    • pp.9-12
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    • 2010
  • The synthesis of $C_{60}$@MWCNT was carried out at room temperature (${\sim}25^{\circ}C$) from arc-discharge prepared Multi-wall carbon nanotubes (MWCNTs). They were oxidized and acid treated for tube opening. Then $C_{60}$ molecules were encapsulated into MWCNTs by wetting them with $C_{60}$-toluene solution for several minutes followed by ultrasonification. $C_{60}$@MWCNT was cleaned by pure toluene to remove any excess $C_{60}$. $C_{60}$@MWCNT was characterized by electron microscopy, which showed large scale filling of $C_{60}$ into MWCNTs. It was observed that the mechanism of insertion of $C_{60}$ into MWCNTs may be due to the capillary suction at the opening ends of MWCNTs.