• Title/Summary/Keyword: ulmus davidiana var. japonica

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Physiological Activity of Extracts of Bark from Ulmus davidiana var. japonica and its fractions (유근피 추출물 및 분획의 생리활성)

  • Yang, Sun a;Kim, A young;Pyo, Byoung sik;Kim, Sun min
    • Korean Journal of Pharmacognosy
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    • v.50 no.2
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    • pp.112-117
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    • 2019
  • In this study, extracts of bark from Ulmus davidiana var. japonica and its fractions were investigated the antioxidative, antibacterial and tyrosinase inhibition activity for physiological activity towards functional applications. In the measurement of DPPH radical scavenging activity, the ethyl acetate fraction showed higher radical scavenging ability than others. Moreover, in the tyrosinase inhibition assay, showed that the ethyl acetate fraction has good inhibition effects. Results of the DPPH radical scavenging and tyrosinase inhibition activity are related with the total polyphenol concentrations of ethyl acetate fraction. In antibacterial activity used to find out by utilizing the disc diffusion assay, chloroform fraction showed strong effect against Staphylococcus aureus and S. epidermidis. These results are related with the flavonoid contents of chloroform fraction. On the other hand, in the L929 cell viability measurement by MTT assay, the hexane, butanol and aqueous fraction treated at high concentration were showed cytotoxicity. But the others samples were exhibited a moderate viabilities. As a result of investigated the antioxidant and tyrosinase inhibition activity, the ethyl acetate fraction could be applicable for cosmetics related fields. And the chloroform fraction showed significant antibacterial activity against S. aureus and S. epidermidis.

Population Genetic Variation of Ulmus davidiana var. japonica in South Korea Based on ISSR Markers (ISSR 표지자를 이용한 느릅나무 자연집단의 유전변이 분석)

  • Ahn, Ji Young;Hong, Kyung Nak;Lee, Jei Wan;Yang, Byung Hoon
    • Journal of Korean Society of Forest Science
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    • v.102 no.4
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    • pp.560-565
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    • 2013
  • Population genetic structure and diversity of Ulmus davidiana var. japonica in South Korea were studied using ISSR markers. A total of 45 polymorphic ISSR amplicons were cropped from 7 ISSR primers and 171 individuals of 7 populations. The average of effective alleles and the proportion of polymorphic loci were 1.5 and 89% respectively. The Shannon's diversity index (I) was 0.435 and the expected heterozygosity from the frequentist's method ($H_e$) and the Bayesian inference (hs) were 0.289 and 0.323 respectively. From AMOVA, 4.2% of total genetic variation in the elm populations was explained with the difference among populations (${\Phi}_{ST}=0.042$) and the other 95.8% was distributed within populations. The ${\theta}^{II}$ value by Bayesian method which was comparable to the FST was 0.043. So the level of genetic diversity in the elm populations was similar to that in Genus Ulmus and the level of genetic differentiation was lower than that of others. No population showed a significant difference in the population-specific fixation indices (average of $PS-F_{IS}=0.822$) or the population-specific genetic differentiations (average of $PS-F_{ST}=0.101$). Seven populations were allocated into 3 groups in the UPGMA and the PCA, but the grouping patterns were different. Also, we could not confirm any geographic trend from Bayesian clustering.

Effect of Soil Conditioner and Loess on Physio-Chemical Properties of Dredged Soil and Seedling Height Growth of Ulmus davidiana var. japonica (토양개량재와 황토가 준설토의 이화학성과 느릅나무의 초기 생장에 미치는 영향)

  • Lee, Hanna;Park, Chanwoo;Lim, Joo-Hoon;Koo, Namin;Lee, Im Kyun
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.18 no.3
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    • pp.1-11
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    • 2015
  • This research was conducted to compare the effect of soil amendment and loess on physial and chemical properties of dredged soil and primary height growth of Ulmus davidiana var. japonica. Three types of research plots: control plot (Dredged soil only), DC treatment plot (Dredged soil + soil amendment) and DCL treatment plot (Dredged soil + soil amendment + Loess) were set at Okgu research site in Saemanguem. 32 trees of U. davidiana var. japonica were planted in each plots after 14 months from the construction. Soil texture, pH, electrical conductivity (EC), organic matter (OM), total nitrogen (TN), available P, exchangeable cation ($K^+$, $Na^+$, $Mg^{2+}$, $Ca^{2+}$) and sodium chloride were measured after 3 years from the construction. Also, survival rate and height of U. davidiana var. japonica were measured 22 months after planting, and the correlation between height of trees and the physico-chemical properties of soil were analyzed. Even though pH in both DC and DCL treatment plots are lower than control plot, they are still alkaline (> pH 8). OM, TN and available P in both DC and DCL treatment were higher than the control. Particularly, the content of available P in both DC and DCL treatment plots are 1.4~5.1 times and 2.0~3.1 times higher than the control respectively. The concentration of exchangeable Mg in DCL treatment plot was 1.1~5.5 times higher than the control (p < .05). The survival rate of the species was the highest in DCL treatment plot (98%) followed by DC treatment plot and the control. The average height of the trees in both DC and DCL treatment plots is 1.1m while the control is 0.8m. OM, TN, available P, K+ were significantly related to the height of U. davidiana var. japonica(p < .01). The results indicate that soil amendment affects on soil physial and chemical properties of dredged soil and height growth of U. davidiana var. japonica.

Effect of Ulmus Davidiana var. Japonica Nakai Extract on Antibiotic Resistant Bacteria in Dyed Cotton (유백피 추출액을 이용한 염색 면포의 항생제 내성균주 증식 억제효능)

  • Choi, Na Young;Kang, Sun-Young
    • Journal of the Korean Society of Clothing and Textiles
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    • v.39 no.2
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    • pp.287-293
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    • 2015
  • This research verifies stainability, super bacteria antibacterial features and antibiotic resistance of Ulmus davidiana var. japonica Nakai (UD) extract in dye cotton cloth. UD was extracted with hot water, the test cloth dyed, and then processed by mordanting treatment using $FeSO_4$ $7H_2O$, $CuSO_4$ $5H_2O$ and $Al_2(SO_4)_3$. The surface color and color fastness of the three kinds (original cloth, the cloth without mordancy and mordant cloth) were measured and the influence of dying of cotton cloth and the mordant method on the genesistasis of Methicillin-resistant Staphylococcus aureus (MRSA), antibiotic resistant bacteria. 1. The surface color of the cotton cloth for dyeing (without and with mordancy) displayed a reddish and yellowish color. Stainability was greatest when the mordant of $FeSO_4$ $7H_2O$ was used. 2. When a mordant was not used for dyeing, the color fastness to washing, perspiration and friction of the contaminated cloth was satisfactory at 4 to 5 grade in general. 3. As for the antibiotic effect to super bacteria, the growth of germ was meaningfully suppressed both on the cloth without and with mordancy compared to the comparison cloth; in addition, the dyeing method with the biggest antibacterial impact was found to be the mordancy with the mordant of $CuSO_4$ $5H_2O$ after dyeing. The results of the experiments that involved dyeing with UD extract showed that cotton cloth processed through mordancy with the mordant of $CuSO_4$ $5H_2O$ had the biggest antibiosis to super bacteria and that processed with the mordant of $FeSO_4$ $7H_2O$ had the greatest stainability.

Anti-Allergic Effect of Ulmus davidiana Cortex on Contact Dermatitis Induced by Dinitrofluoro-Benzene in Mice

  • Lyu, Jeonghyeon;Kim, Byung-Joo;Kim, Hyungwoo
    • Journal of Pharmacopuncture
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    • v.16 no.2
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    • pp.41-45
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    • 2013
  • Objective: The root bark of Ulmus davidiana var. Japonica (Ulmi Radicis cortex, URC) is a medicinal herb used for promoting diuresis and treating dampness. In Korea, URC has long been used as an efficacious therapy for inflammation, burns, frostbite and skin diseases such as eczema and psoriasis. Methods: In the present study, we used 1-fluoro-2,4-dinitrofluorobenzene (DNFB)-induced contact dermatitis (CD) mouse model to investigate the anti-allergic and the anti-inflammatory effects of URC on skin lesion, histopathological changes and specific antibody production. Results: URC treatment, 10 mg/mL, effectively inhibited skin lesions induced by repeated paintings with DNFB. In the histopathological observation, topical application of URC inhibited spongiosis. In addition, URC lowered the production levels of total immunoglobulin and IgG2a in serum. Conclusion: These data indicate that URC has an anti-inflammatory effect that produces an improvement of skin lesions in CD mice.

In vivo Antitumor Activity of Mansonone E isolated from Ulmus davidiana var. japonica NAKAI (느릅나무의 근피로부터 분리한 Mansonone E의 항암효과)

  • Cho, Choa-Hyung;Yoon, Won-Ho;Lee, Keyong-Ho
    • Korean Journal of Pharmacognosy
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    • v.35 no.3 s.138
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    • pp.199-202
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    • 2004
  • A highly potent antitumor sesquiterpene O-naphthoquionone has been isolated from Ulmus davidiana, which has been traditionally used as the folk medicine. The structure of this compound was established on the basis of spectral data obtained from UV, IR, MS, and NMR spectrometry, and determined as mansonone E. This compound showed potent antitumor activity on in vivo assay, hollow fiber and xenograft assay.

Distribution of the Genetic Resource and the Biomass of Root Bark of Ulmaceae Species

  • Park, Dong Jin;Yong, Seong Hyeon;Yang, Woo Hyeong;Seol, Yuwon;Choi, Eunji;Kim, Hyeong Ho;Ahn, Mi-Jeong;Choi, Myung Suk
    • Journal of agriculture & life science
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    • v.53 no.2
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    • pp.65-75
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    • 2019
  • Stem and root of elm trees have used as traditional medical materials, but there is little information on the distribution and resources of habitats. Korean native growing Ulmus spp. (U. davidiana var. Japonica, U. parvifolia, U. davidiana, and U. macrocarpa) genetic resources studied through The National Forest Inventory of Korea data and field survey. The distributions of U. davidiana var. japonica according to elevation distributed evenly. Both U. parvifolia and U. davidiana were inhabited mostly at less than 200 m of altitude. Each Ulmaceae species widely were distributed nationwide, but a dominant species was different depending on locals. It observed that Ulmaceae inhabits mainly in steep slopes of 31-45 degrees. Most of the habitats regenerated by natural seeding and the most abundant species were a codominant tree. Distribution of trees in U davidiana var. japonica was 7 m-13 m, and in young U. parvifolia and U. macrocarpa, more than 25% of young trees less than 7 m observed. The distribution of the diameter of breast height of the U. davidiana var. japonica was 46.4% for 11-20 cm, 52.6% for 11-20 cm in U. parvifolia. The average T/R ratio was 0.83, and the mean weight ratio of root bark was 62%. As the results of this study, the domestic Ulmaceae biomassare very small. It is difficult to harvest in that the habitat on the slope. Thus, it is too hard to develop functional materials using biomass at present. Therefore, it is necessary to develop technology for the selection and propagation of elite trees of Ulmaceae.

Change in the Seed Characteristics and Germination Properties of Ulmus davidiana var. Japonica According to Seed Collection Time (채취 시기에 따른 느릅나무의 종자 형질 및 발아 특성 변화)

  • Tak, Woo Sik;Choi, Chung Ho;Kim, Tae Su
    • Journal of Korean Society of Forest Science
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    • v.95 no.3
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    • pp.316-322
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    • 2006
  • This study was conducted to examine the changes of seed characteristics and germination properties and to determine the most suitable collection time in Ulmus davidiana var. japonica. Seeds were collected six times with the interval of seven days after April 21, 2005 at Jeong-seon. There were differences in seed characteristics and germination properties among seed collection times. The sizes of seed and fruit increased with the collection time, but seed moisture contents decreased with the collection time after increasing until May 5. The 1,000 seeds weight decreased with the collection time after increasing until May 12. Collection time showed high positive correlations (r=0.790, p<0.01) with seed size and negative correlations (r=-0.919, p<0.01) with moisture contents. Percent germination (PG) presented the highest value in seeds collected at May 5, and decreased with the collection time. Mean germination time(MGT), germination speed(GS), and germination performance index(GPI) also showed the highest values in seeds collected at May 5. PG, MGT, and GS had correlations with other seed characteristics except the 1,000 seed weight. The relative growth rate of height and root collar diameter of seedlings presented the highest values in seedling germinated from seed collected at May 5, and seedlings germinated from seeds collected at May 5 and 12 showed the highest values in chlorophyll contents. Consequently it will be possible to product a number of seedlings when the seeds of U. davidiana var. japonica are collected just before drying morphologically.

Separation and purification of substance having matrix metalloproteinase-9 inhibition effect in Ulmus davidiana Plancn. var. japonica Nakai (유근피(楡根皮)에 존재하는 matrix metalloproteinase-9 억제 물질의 분리 및 정제)

  • Han, Kee-Jung;Lee, Kwang-Soo;Kong, Kwang-Hoon;Cho, Sung-Hye
    • Analytical Science and Technology
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    • v.16 no.3
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    • pp.179-184
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    • 2003
  • Root bark of Ulmus davidiana Plancn. var. japonica Nakai was extracted by using several solvents with different polarities. Each extract was treated on the MMPs obtained from SK-Hep-1 in order to investigate inhibition effect. Zymography of MMPs showed that MeOH extract has significant inhibition effect. On the GC-MS analysis the highest mass to charge ratio (m/z) of the purified substance was 281. Also, on zymography of MMPs the substance showed 47% inhibition effect at the concentration of $314.7{\mu}g/g$. Cell viability of SK-Hep-1 was 60% at $31.47{\mu}g/g$.