• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.6
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• pp.454-458
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• 2004

A study of the POSE (predispersed solvent extraction) for succinic acid by colloidalliq­uid aphrons was conducted. The organic phase contaning TOA (tri-n-octylamine) and 1-octanol permits a selective extraction of succinic acid from its aqueous solution. There was no difference of the extractability of POSE and that of conventional mixer-settler type extraction. Taking into account the no mechanical mixing in POSE, it was concluded that the POSE process is more adaptive than the conventional mixer-settler type extraction process. From mass transfer analysis at the various concentration of TOA in counter-current continuous operation, the concentration of TOA had no influence on the mass transfer coefficient. The loading values in continuous POSE were almost same as those in batch operation.

• Proceedings of the Korea Information Processing Society Conference
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• 2015.10a
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• pp.865-866
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• 2015

스마트폰은 주로 사용되고 있는 안드로이드 OS는 다양한 악성코드로 인해 금전적 피해, 데이터 유출 및 통제권한 상실 등과 같은 많은 피해를 당하고 있다. 침해 위협을 가중시키고 있는 모바일 악성코드 중 심각한 피해를 유발하는 커널 기반의 루팅(Rooting) 악성코드는 일반적인 탐지 방법으로는 찾아낼 수 없는 어려움이 있다. 본 논문에서는 커널 기반에서 동작하는 루팅(Rooting) 악성코드를 탐지하기 위한 방법을 제안한다. 스마트폰 어플리케이션이 실행될 때마다 생성되는 모든 프로세스의 UID를 확인하여 비정상적으로 사용자(User) 권한에서 관리자(Root) 권한으로 변환되는지를 확인하는 방법이다. 제안하는 방법을 활용하여 알려지지 않은 악성코드로 인한 안드로이드 OS의 피해를 최소화할 수 있을 것으로 기대된다.

• Korean Journal of Veterinary Service
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• v.28 no.4
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• pp.407-412
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• 2005

Ten isolates of Verotoxin-producing Escherichia coli (VTEC) were detected in slaughtered cattle and investigated their phenotypic characteristics and antimicrobial susceptibility. None of the isolates was positive for eae gene. Only one isolate was positive for uidA gene. Eight out of ten isolates of VTEC were originated from broker's cattle. Thus microbiological monitoring for broker farms should be performed to minimize VTEC contamination. In the antimicrobial susceptibility test, all the isolates were highly resistant to bacitracin and lincomycin whilst they are susceptible to apramycin and neomycin.

• The Journal of Society for e-Business Studies
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• v.24 no.2
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• pp.199-216
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• 2019

The public SW market is 3 trillion won, which is less than 10% of the total SW market. However, due to the nature of the domestic market, it is an important market with a relatively large impact on small and medium-sized software companies. In this market, government is operating the Public SW Project Demand Forecasting System in order to support the marketing activities of small and medium sized SW companies and establish a fair market order. The current system has limitations such as lack of user convenience, insufficient analysis capability and less business connection. This study was conducted to identify the problems of these systems and to propose a new system for improving the convenience of users and expanding the information utilization of SMEs. To this end, we analyzed the requirements of each stakeholder. We proposed the 2-phased forecasting cycle, the management cycle, and the system life cycle of public SW projects and created a unified identifier (UID) so that the information of those projects can be identified and linked among them. As a result, an integrated reference model of project information management based on system life cycle was developed, which can explain the demand forecasting and project information, and the improved processes was also designed to implement them. Through the result of this study, it is expected that integrated management of public SW projects will be possible.

• Journal of Plant Biotechnology
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• v.37 no.3
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• pp.319-326
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• 2010

An interest in the production of seed-oil based fuel and raw materials, which comes from renewable plant sources, has been intrigued by the phenomenon of global warming and shortage of fossil fuels. Rapeseed (Brassica napus) is the most important oilseed crop, which produces seeds with 40% oil. It is desirable to develop genetically modified rapeseed producing oils, which can be easily converted to biodiesel. As an initial step for development of genetically modified rapeseed for the production of biofuels or bio-based materials, Korean rapeseed cultivars, Naehan, Youngsan, Tammi and Halla, were analyzed. Four Korean rapeseed cultivars produce 32 to 40% oil of seed dry weight, which is rich in oleic acid (more than 60 mole%). The cotyledonary petioles of rapeseed cultivar, Halla, were transformed using Agrobacterium tumefaciens strain GV3101, carrying the uidA gene encoding $\beta$-glucuronidase (GUS) as a reporter gene and the phosphinothricin acetyltransferase (PAT) gene as a selectable marker. The stable integration of PAT gene in the genome of transgenic rapeseeds was confirmed by PCR analysis. Expression of uidA gene in various rapeseed organs was determined by fluorometric assay and histochemical staining. Transformation efficiency of a Korean rapeseed Halla cultivar was 10.4%. Genetic inheritance of transgenes was confirmed in $T_2$ generation.

• Journal of Food Hygiene and Safety
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• v.26 no.2
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• pp.114-119
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• 2011

In this study, two duplex real-time PCR approach with melting curve analysis is presented for the detection of Escherichia coli O157:H7, Listeria monocytogenes, Salmonella spp. and Staphylococcus aureus, which are important food-borne bacterial pathogens usually present in fresh and/or minimally processed vegetables. Reaction conditions were adjusted for the simultaneous amplification and detection of specific fragments in the ${\beta}$-glucuronidase (uidA, E. coli), thermonuclease (nuc, S. aureus), hemolycin (hly, L. monocytogenes) and tetrathionate reductase (ttr, Salmonella spp.) genes. Melting curve analysis using a SYBR Green I real-time PCR approach showed characteristic $T_m$ values demonstrating the specific and efficient amplification of the four pathogens; $80.6{\pm}0.9^{\circ}C$, $86.9{\pm}0.5^{\circ}C$, $80.4{\pm}0.6^{\circ}C$ and $88.1{\pm}0.11^{\circ}C$ for S. aureus, E. coli O157:H7, L. monocytogenes and Salmonella spp., respectively. For all the pathogens, the two duplex, real-time PCR was equally sensitive to uniplex real-time PCR, using same amounts of purified DNA, and allowed detection of 10 genome equivalents. When our established duplex real-time PCR assay was applied to artificially inoculated fresh lettuce, the detection limit was $10^3$ CFU/g for each of these pathogens without enrichment. The results from this study showed that the developed duplex real-time PCR with melting curve analysis is promising as a rapid and cost-effective test method for improving food safety.

• The Journal of the Korea institute of electronic communication sciences
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• v.8 no.12
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• pp.1891-1904
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• 2013

In RFID System, one of the problem that we must slove is to devise a good anti-collision algorithms to improve the efficiency of tag identification which is usually low because of tag collision. Among of the existing RFID anti-collision algorithm, BS (Binary Search) algorithm, though simple, has a disadvantage that the stage 0f times used to identify the tags increase exponentially as the number of tags does. In this Paper, I propose a new anti-collision algorithm called Multi-collision reflected frame which restricts the number of stages and decided bit. Since the proposal algorithm keep the length size of UID and density of total tag when have 100%.

• The Journal of the Korea institute of electronic communication sciences
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• v.7 no.2
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• pp.235-242
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• 2012

In RFID System, one of the problem that we must slove is to devise a good anti-collision algorithms to improve the efficiency of tag identification which is usually low because of tag collision. Among of the existing RFID anti-collision algorithm, BS (Binary Search) algorithm, though simple, has a disadvantage that the stage of times used to identify the tags increase exponentially as the number of tags does. In this paper, I propose a new anti-collision algorithm called Multi-collision reflected frame which restricts the number of stages and decided bit. Since the proposal algorithm keep the length size of UID and density of total tag when have 100%. The simulation results showed that the proposed algorithm improves the efficiency by 30~50% compared to the existing algorithm.

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.1
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• pp.69-76
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• 1998

This study was conducted to construct of the transgenic plants wliich are resistant to oxidative stresses including ozone with B. mpestris cytosolic glutathione reductase cDNA using the binary vector system of Agrobacterium tumefaciens. The 1.8kb B. campestris cytosolic GR cDNA was subcloned into the unique Sma I site of the plant transformation vector pBKSI- I, downstream of the constitutive CaMV 35s promoter and upstream of the nos termination sequence, in place of the uidA (GUS) reporter gene. The resulting plant transformation vector, pBKS-GRI, was introduced into A. tumefaciens LBA4404 by two cycles of tkeze-thaw method. The B. nqus cotyledonary petioles were transformed by the Agrubaferium harboring pBKS-GRI. Transformed shoots were induced and selected on regeneration medium supplemented with kanarnycin. The shoot formation was increased remarkably by addition of Ag$NO_3$, in MS media. The transgenic plants were analyzed for the presence of the B. campestris GR gene by Southern blot analysis and it was confirmed that a foregin gene was stably integrated into the genomes of B. nqus plants.

• Plant Biotechnology Reports
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• v.4 no.2
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• pp.129-138
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• 2010

An efficient transformation system for high-throughput functional genomic studies of kiwifruit has been developed to overcome the problem of necrosis in Actinidia arguta explants. The system uses Agrobacterium tumefaciens strain EHA105 harbouring the binary vector pART27-10 to inoculate leaf strips. The vector contains neomycin phosphotransferase (nptII) and ${\beta}$-glucuronidase (GUS) (uidA) genes. A range of light intensities and different strengths of Murashige and Skoog (MS) basal salt media was used to overcome the problem of browning and/or necrosis of explants and calli. Callus browning was significantly reduced, resulting in regenerated adventitious shoots when the MS basal salt concentration in the culture medium was reduced to half-strength at low light intensity ($3.4\;{\mu}mol\;m^{-2}\;s^{-1}$) conditions. Inoculated leaf strips produced putative transformed shoots of Actinidia arguta on half-MS basal salt medium supplemented with 3.0 $mg\;l^{-1}$ zeatin, 0.5 $mg\;l^{-1}$ 6-benzyladenine, 0.05 $mg\;l^{-1}$ naphthalene acetic acid, 150 $mg\;l^{-1}$ kanamycin and 300 $mg\;l^{-1}$ $Timentin^{(R)}$. All regenerated plantlets were deemed putativ transgenic by histochemical GUS assay and polymerase chain-reaction analysis.