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http://dx.doi.org/10.5010/JPB.2010.37.3.319

Development of herbicide-tolerant Korean rapeseed (Brassica napus L.) cultivars  

Kim, Hyo-Jin (Department of Bioenergy Science & Technology, Chonnam National University)
Lee, Hye-Jin (Department of Bioenergy Science & Technology, Chonnam National University)
Go, Young-Sam (Department of Plant Biotechnology, Chonnam National University)
Roh, Kyung-Hee (National Academy of Agricultural Science, Rural Development Administration)
Lee, Young-Hwa (Bioenergy Crop Research Center and National Institute of Crop Science, Rural Development Administration)
Jang, Young-Seok (Bioenergy Crop Research Center and National Institute of Crop Science, Rural Development Administration)
Suh, Mi-Chung (Department of Bioenergy Science & Technology, Chonnam National University)
Publication Information
Journal of Plant Biotechnology / v.37, no.3, 2010 , pp. 319-326 More about this Journal
Abstract
An interest in the production of seed-oil based fuel and raw materials, which comes from renewable plant sources, has been intrigued by the phenomenon of global warming and shortage of fossil fuels. Rapeseed (Brassica napus) is the most important oilseed crop, which produces seeds with 40% oil. It is desirable to develop genetically modified rapeseed producing oils, which can be easily converted to biodiesel. As an initial step for development of genetically modified rapeseed for the production of biofuels or bio-based materials, Korean rapeseed cultivars, Naehan, Youngsan, Tammi and Halla, were analyzed. Four Korean rapeseed cultivars produce 32 to 40% oil of seed dry weight, which is rich in oleic acid (more than 60 mole%). The cotyledonary petioles of rapeseed cultivar, Halla, were transformed using Agrobacterium tumefaciens strain GV3101, carrying the uidA gene encoding $\beta$-glucuronidase (GUS) as a reporter gene and the phosphinothricin acetyltransferase (PAT) gene as a selectable marker. The stable integration of PAT gene in the genome of transgenic rapeseeds was confirmed by PCR analysis. Expression of uidA gene in various rapeseed organs was determined by fluorometric assay and histochemical staining. Transformation efficiency of a Korean rapeseed Halla cultivar was 10.4%. Genetic inheritance of transgenes was confirmed in $T_2$ generation.
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