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Immobilization of Cyclodextrin Glucanotrasferase on Amberline IRA-900 for Biosynthesis of Transglycosylated Xylitol

  • Kim, Pan-Soo;Shin, Hyun-Dong;Park, Joong-Kon;Lee, Young-Hyun
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.3
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    • pp.174-180
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    • 2000
  • Cyclodextrin glucanotransferase (CGTasa) from Thermoanaerobacter sp. was adsorbed on the ion exchange resin Amberlite IRA-900. The optimum conditions for the immobilization of the CGTase were pH6.0 and 600 U CGTase/g resin, and the maximum yield of immobilization was around 63% on the basis of amount ratio of the adsorbed enzyme to intial amount in the solution. Immobilixation of CGTase shifted the optimum temperature for the enzyme to peoduce transglycosylated xylitol from 7$0^{\circ}C$ to 9$0^{\circ}C$ and improved the thermal stability of immobilized CGTase, especially after the addition of soluble starch and calcium ions. Transglycosylated xylitol was continuoncly produced using immobilized CGTase in the column type packed bed reactor, and the operating conditions for maximum yield were 10%(w/v) dextrin (13 of the dextrose equivalent) as the glycosyl donor, 10%(w/v) dextrin (13 of the dextrose equivalent) as the glycosyl donor, 10%(w/v) xylitor as the glycosyl acceptor, 20mL/h of medium fiow rate, and 6$0^{\circ}C$. The maximum yield of transglycosylated xylitol and productivity were 25% and 7.82 g.L-1.h-1, respectively. The half-life of the immobilized CGTase in a column type packed bed reactor was longer than 30 days.

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CMP of BTO Thin Films using Mixed Abrasive slurry (연마제 첨가를 통한 BTO Film의 CMP)

  • Kim, Byeong-In;Lee, Gi-Sang;Park, Jeong-Gi;Jeong, Chang-Su;Gang, Yong-Cheol;Cha, In-Su;Jeong, Pan-Geom;Sin, Seong-Heon;Go, Pil-Ju;Lee, U-Seon
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2006.05a
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    • pp.101-102
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    • 2006
  • BTO ($BaTiO_3$) thin film is one of the high dielectric materials for high-density dynamic random access memories (DRAMs) due to its relatively high dielectric constant, It is generally known that BTO film is difficult to be etched by plasma etching, but high etch rate with good selectivity to pattern mask was required. The problem of sidewall angle also still remained to be solved in plasma etching of BTO thin film. In this study, we first examined the patterning possibility of BTO film by chemical mechanical polishing (CMP) process instead of plasma etching. The sputtered BTO film on TEOS film as a stopper layer was polished by CMP process with the sell-developed $BaTiO_3$- and $TiO_2$-mixed abrasives slurries (MAS). respectively. The removal rate of BTO thin film using the $BaTiO_3$-mixed abrasive slurry ($BaTiO_3$-MAS) was higher than that using the $TiO_2$-mixed abrasive slurry ($TiO_2$-MAS) in the same concentrations. The maximum removal rate of BTO thin film was 848 nm/min with an addition of $BaTiO_3$ abrasive at the concentration of 3 wt%.

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A Study on the Mechanical Properties of Carbon Fiber Reinforced Cement Composite Impregnated in Polymer (폴리머 함침 탄소섬유보강 시멘트 복합체의 역학적 특성에 관한 연구)

  • ;;Lee, Burtrand. I.
    • Magazine of the Korea Concrete Institute
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    • v.4 no.1
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    • pp.107-118
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    • 1992
  • In order to examine the mechanical properties of carbon fiber reinforced cement composites with silica powder PAN - based carbon fiber and Pitch- based carbon fiber, and polymer impregnators experimental studies on CFRC impregnated in polymer were carried out. The effects of types, length, and content~i of carbon fibers and matrices of fresh and hardened CFRC impregnated in polymer were examined. The test results show that compressive, tensile, and flexural strength of CFRC impregnated in polymer were much more iriCreased than those of air cured and autodaved CFIIC CFRC impregnated in polymer was also considerably effective in improving toughness, freeze thaw resistance, loss of shrinkage, and creep resist ance, compared with air cured and autoclaved CFRC.

A Research for Web Documents Genre Classification using STW (STW를 이용한 웹 문서 장르 분류에 관한 연구)

  • Ko, Byeong-Kyu;Oh, Kun-Seok;Kim, Pan-Koo
    • Journal of Information Technology and Architecture
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    • v.9 no.4
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    • pp.413-422
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    • 2012
  • Many researchers have been studied to reveal human natural language to let machine understand its meaning by text based, page rank based or more. Particularly, it has been considered that URL and HTML Tag information in web documents are attracting people' attention again to analyze huge amount of web document automatically. In this paper, we propose a STW (Semantic Term Weight) approach based on syntactic and linguistic structure of web documents in order to classify what genres are. For the evaluation, we analyzed more than 1,000 documents from 20-Genre-collection corpus for training the documents based on SVM algorithm. Afterwards, we tested KI-04 corpus to evaluate performance of our proposed method. This paper measured their accuracy by classifying them into an experiment using STW and one without u sing STW. As the results, the proposed STW based approach showed approximately 10.2% which Is higher than one without use of STW.

Design of Object-Active-Knowledge(OAK) Model for Postal Business (객체-능동-지식 기반 우편 업무 모델 설계)

  • Lee, Jae-Ho;Sin, Pan-Seop;Im, Hae-Cheol;Hwang, Jae-Gak;U, Dong-Jin;Kim, Hye-Gyu
    • The Transactions of the Korea Information Processing Society
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    • v.4 no.8
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    • pp.1907-1920
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    • 1997
  • In this paper, we present integrated model of postal business that would be newly organized. The integrated model is developed through four phase. First, postal business is analyzed by three parts;collection, transportation and delivery. Second, the modeling criteria are developed that will be used for designing integrated postal business model. Third, object-oriented concepts are used by the steps of postal business modeling criteria captured. Finally, methods applied to developed model are grouped according to their function. Also, active-based mechanisms such as trigger and constraints are developed, and knowledge-based mechanisms such as inference are developed. These selected methods and attributes are encapsulated into objects. These constitute an object-active-knowledge based database model. Finally, Operational Scenario and Service Scenario are composed for real application according to proposed O-A-K model.

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Performing Inauthenticity: The Crisis of Asian America and Alternative Identity Politics ("가짜로 살아가기" -정체성으로서의 '아시아계 미국인'의 위기와 대안)

  • Im, Kyeong Kyu
    • Journal of English Language & Literature
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    • v.56 no.5
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    • pp.773-796
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    • 2010
  • This essay examines, first, the possibility and limitation of Asian America as a category of identity and its political and cultural implications through various theoretical perspectives. Here, by closely reading David Mura's poem "The Colors of Desire," I will argue that "Asian America" as a category of identity is now on the verge of falling apart and its politics of identity is no longer an effective way of fighting back against racism in the US. It is because Asian America is indeed what might be called a historical block, a product of ad-hoc coalition between different ethnic groups historically situated and constructed. In this sense, it is a kind of phantasmal object that is marked by practical absence. This fabricatedness inherent in Asian America as an identity category signifies that it has no essence that is meant to define the group in a transcendental way. The internal totality and coherence of that identity can thus be achieved only by suppressing differences between various ethnic groups and positing a single 'authentic' Asian American identity and culture. More dangerously, according to Viet Nguyen, such idealization of a single subject position can reinforces ideological rigidity that might threaten the ability of Asian America to represent itself in a unified fashion. Then, he predicts, Asian America will lose its cohesive force and fall apart. Eventually, every group within Asian America will be ethnicized. The only way of escaping from this bleak situation, as Vincent Cheng argues, is to foregroud the fabricatedness and ad-hocness of Asian America and to perform "inauthenticity," because Asian America is nothing but a functional category that is marked by absence of essence or authenticity. If Asian Americans admit that they have no essence and that they are essentially inauthentic, the practice of performing inauthenticity can become what we might call an alternative Asian American culture and identity.

The development and effects of an online-based community psychiatric nursing practice program with the ARCS model (ARCS모형 적용 온라인 기반 지역사회정신간호학실습 프로그램 개발 및 효과)

  • Kim, Pan Heui;Kim, Hee Sook
    • The Journal of Korean Academic Society of Nursing Education
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    • v.30 no.1
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    • pp.5-18
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    • 2024
  • Purpose: This study aimed to identify whether there is a difference between an online-based community psychiatric nursing practice program with the ARCS model and a conventional community psychiatric nursing practice program in promoting nursing students' learning motivation, knowledge of community psychiatric nursing, communication skills, and learning self-efficacy. Methods: This study used a quasi-experimental design with a non-equivalent control group. The participants were 44 nursing students at three nursing colleges in Gyeongsangbuk-do. The experimental group was provided the online-based community psychiatric nursing practice program with ARCS model, while the control group was provided the conventional community psychiatric nursing practice program from July 9, to September 3, 2022. Both groups received practice training 8 hours a day, 5 days two weeks. The collected data were analyzed using the exact χ2, Mann-Whitney U-test, and Quade's two-way ANCOVA with the IBM SPSS Statistics 28.0 program. Results: The results of the comparison of an experimental group training with the online-based community psychiatric nursing practice program with ARCS model and a control group training with the conventional community psychiatric nursing practice program showed that, there was no statistically significant difference between the two groups in learning motivation knowledge of community psychiatric nursing, and learning self-efficacy. However, communication skills were statistically significantly higher in the experimental group (F=6.23, p=.017). Conclusion: The online-based community psychiatric nursing practice program with ARCS model can be used as a substitute learning to improve community psychiatric nursing capabilities in situations when clinical practice is suspended due to infectious diseases such as coronavirus disease 2019 or when is a shortage of community psychiatric nursing practice institutions.

Variation of Meat Quality Parameters Due to Conformation and Fat Class in Limousin Bulls Slaughtered at 25 to 27 Months of Age

  • Guzek, Dominika;Glabska, Dominika;Pogorzelski, Grzegorz;Kozan, Karolina;Pietras, Jacek;Konarska, Malgorzata;Sakowska, Anna;Glabski, Krzysztof;Pogorzelska, Ewelina;Barszczewski, Jerzy;Wierzbicka, Agnieszka
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.5
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    • pp.716-722
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    • 2013
  • The aim of the present study was to determine the influence of age of animal, hot carcass weight, pH, conformation and fat class on basic beef quality attributes (tenderness, sarcomere length, basic chemical composition, marbling and colour) in a group of purebred animals. The object of the study was beef of Limousin bulls (25 to 27 months, hot carcass weight - $432{\pm}31$ kg, "U" conformation class, "2"-"3" fat class). Analysed cuts were Infraspinatus muscle from the blade and Longissimus dorsi muscle from the cube roll and the striploin. Tenderness was analysed with universal testing machine, colour - chromometer analysis, sarcomere length - microscopic method, basic chemical composition - near-infrared spectroscopy and marbling - computer image analysis. No differences in tenderness and sarcomere length were observed within the age groups of Limousin bulls (age of 25, 26, 27 months) (p>0.05). Moisture (p = 0.0123) and fat (p = 0.0250) content were significantly different for meat of animals slaughtered at the age of 25 and 27 months. No influence of pH value on tenderness was observed, but at the same time, influence on sarcomere length (p = 0.039) and $b^*$ component of colour (0.045) was found. For "U" conformation class, in subclasses, as well as for fat classes "2"-"3", there were no differences in tenderness, sarcomere length and colour components. Higher fat content was observed in the higher fat class, rather than in lower, however this feature was not associated with marbling.

Managerial Ownership and R & D Investment in the Chinese Firms : Comparison between State_Owned Firms and Private_Owned Firms (경영자 지분이 연구개발투자에 미치는 영향: 중국 국유기업과 민영기업 비교를 중심으로)

  • Cho, Young-Gon;Zhou, Xiao Long;Zhang, Xiao Pan
    • The Journal of the Korea Contents Association
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    • v.17 no.5
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    • pp.8-17
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    • 2017
  • Using 1855 observations from 5 years-371 firms panel data during 2010 to 2014 in Chinese stock exchanges, this study examines the impact of managers' ownership on R & D expenditures. The empirical study finds that when firms are state-owned, managers' ownership have negative relation with the level of R & D expenses as well as the likelihood of executing R & D investment, implying that managers are less likely to invest in high risky projects due to managerial ownership's entrenchment effects to pursue private benefits rather than alignment of interest effect as shareholders. The empirical study also finds that when firms are private-owned, managerial ownership are inverse U shaped related to the level of R & D expenses, implying that managers are less likely to invest in high risky projects due to increasing risk aversion resulting from concentration of private wealth at its high level while managers are more likely to invest in high risky projects due to increasing incentives as shareholders at its low level. The results support that the effects of managerial ownership on R & D expenses may be different according to the ownership type of Chinese listed firms.

Semantic Topic Selection Method of Document for Classification (문서분류를 위한 의미적 주제선정방법)

  • Ko, kwang-Sup;Kim, Pan-Koo;Lee, Chang-Hoon;Hwang, Myung-Gwon
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.11 no.1
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    • pp.163-172
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    • 2007
  • The web as global network includes text document, video, sound, etc and connects each distributed information using link Through development of web, it accumulates abundant information and the main is text based documents. Most of user use the web to retrieve information what they want. So, numerous researches have progressed to retrieve the text documents using the many methods, such as probability, statistics, vector similarity, Bayesian, and so on. These researches however, could not consider both the subject and the semantics of documents. As a result user have to find by their hand again. Especially, it is more hard to find the korean document because the researches of korean document classification is insufficient. So, to overcome the previous problems, we propose the korean document classification method for semantic retrieval. This method firstly, extracts TF value and RV value of concepts that is included in document, and maps into U-WIN that is korean vocabulary dictionary to select the topic of document. This method is possible to classify the document semantically and showed the efficiency through experiment.