• Mycobiology
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• 제34권3호
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• pp.114-119
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• 2006

Biochemical variability among 20 Indian isolates of Sclerotinia sclerotiorum collected from different hosts/soil samples from different localities in India is reported. High Performance Liquid Chromatographic (HPLC) analysis of ethyl acetate fraction of culture filtrate, mycelia, sclerotia and sclerotial exudate showed $15{\sim}23$ peaks but only 11 could be identified. They were tannic, gallic, oxalic, caffeic, vanillic, ferulic, O-coumeric, chlorogenic, cinnamic, salicylic and gentisic acids. The amount of phenolic compounds varied among the culture filtrates, mycelia, sclerotia and sclerotial exudates of S. sclerotiorum.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2002년도 Molecular and Cellular Response to Toxic Substances
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• pp.163-163
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• 2002

4-tert-octylphenol (OP) is surfactant additive widely used in the manufacture of a variety of detergents and plastic products, a U.S. Environmental Protection Agency (EP A) classified endocrine disruptor. The purpose of this study was developed the analytical method of OP and determined toxicokinetics parameters after i. v. and oral administration in SD rats plasma concentration of OP using HPLC.(omitted)

• 대한화장품학회지
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• 제16권1호
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• pp.40-46
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• 1990

A method was described for the analysis of Artemisia extract in cosmetic soap. Esculetin-6-methylether was used as indicator ingredient for analysis of Artemisia extract. It was isolated from the plant and purified with silicagel column. The structure was conformed with IR, NMR and MASS spectroscopy. The Artemisia extract in sample was isolated with chloroform and interfering com- pounds were eliminated with silicagel column. The Artemisia extract was determined by reverse phase high- performance liquid chromatography with a fluorescence detector and a solvent system of H2O/THF/MeOH. The recorveries of Artemisia extract were 93.9-106.1% in the cosmetic soap.

• Nutrition Research and Practice
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• 제14권5호
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• pp.463-477
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• 2020

BACKGROUND/OBJECTIVES: Many studies have suggested that Rhus verniciflua Stokes (RVS) and its extract are anticancer agents. However, RVS had limited use because it contains urushiol, an allergenic toxin. By improving an existing allergen-removal extraction method, we developed a new allergen-free Rhus verniciflua Stokes extract (RVSE) with higher flavonoid content. In this study, we examined whether RVSE inhibits the ability of AGS gastric cancer cells to migrate and invade. MATERIALS/METHODS: The flavonoids content of RVSE was analyzed by HPLC. The effects of RVSE on migration and invasion in AGS cells were analyzed by each assay kit. Matrix metalloproteinase (MMP)-9, plasminogen activator inhibitor-1 (PAI-1) and urokinase-type plasminogen activator (uPA) protein expression was analyzed by protein antibody array. The Phosphorylation of signal transducer and activator of transcription (STAT) 3 were assayed by Western blot analysis. RESULTS: RVSE treatment with 0-100 ㎍/mL dose-dependently reduced the ability of AGS cells to migrate and invade. Notably, treatment with RVSE strongly inhibited the expression of MMP-9 and uPA and the phosphorylation of STAT3. In contrast, RVSE treatment dramatically increased the expression of PAI-1. These results indicate that the inhibition of MMP-9 and uPA expression and STAT3 phosphorylation and the stimulation of PAI-1 expression contributed to the decreased migration and invasion of AGS cells treated with RVSE. CONCLUSIONS: These results suggest that RVSE may be used as a natural herbal agent to reduce gastric cancer metastasis.

• 동의신경정신과학회지
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• 제7권1호
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• pp.39-48
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• 1996

This study was performed to investigate the effects of Qigong training after physical training stress in untrained college students For this study, 6 voluntary subjects(male 4, female 2) were chosen in untrained students of K University. they were trained by teachers during 6 weeks and tested just before Qingong training and after 6 weeks. Each subject was performed a treadmill exercise(model Q65, Quinton Co, U.S.A.) to the all-out state. During exercise stress test, electrocardiogram, heart rate were checked by stress test monitor(model Q4500, Quinton Co, U.S.A) and also oxygen uptake, maximal oxygen uptake analyzed continuously by automatic gas analysis(model QMC, Quinton Co, U.S.A). During physical training the serum were collected 3 times, pre-experimental rest time, and serum catecholamine were measured by HPLC.T-test of statistical analysis system was used in every experiment for statistical assessment. The results of T-test on these data were summarized as follow:1.Heart rate change during exercise stress test after Qigong training was shown more decreasing tendency than before training. Especially, heart rate change after Qigong training during resting periods was decreased significantly than before training.2. Oxygen uptake change during exercise stress test after Qigong training was shown more increasing tendency than before training, And also maximal oxygen uptake after Qigong training was shown more increasing tendency than before 6 weeks.3. Epinephrine level of after Qigong training was more decreased significantly than before training in all-out state. And norepinephrine level of after Qigong training was shown more decreasing tendency than before training in all-out state and after 30 minutes rest time. Above results indicate that Qigong training for 6 weeks could be effective to elevate the cardiopulmonary functions and diminish the stress responses of the physical stress.

• 농업과학연구
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• 제38권1호
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• pp.87-94
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• 2011

Flavonoid contents of common buckwheat (cv. Kitawase) and tartary buckwheat (cv. Hokkai T 8, Hokkai T 9 and Hokkai T 10) were determined by high-performance liquid chromatography (HPLC). Moreover, they were measured at different plant developments such as 10, 18, 20, 22 and 30 days after sowing (DAS) and with plant parts including leaf, stem and flower harvested at 30 DAS. Total flavonoids including chlorogenic acid, four kinds of C-glycosylflavones (orientin, isoorientin, vitexin, isovitexin) and rutin of tartary buckwheats (range of 44.2-54.7, mean 44.2) were found 35% higher than those of common buckwheat (28.9 mg/g dry wt.). Among them, rutin was measured above 80% of total flavonoid contents. The other flavonoids (chlorogenic acid and four kinds of C-glycosylflavones) presented the highest level at 10 DAS and decreased according to plant developments. On the other hand, rutin content of Kitawase presented the highest level (33.6 mg/g dry wt.) at 22 DAS and decreased up to 30 DAS. Rutin content in tartary buckwheat temporarily decreased from 10 to 18 DAS and then reversely increased up to 30 DAS presented the highest level as 'U' curve. In Hokkai T 10, rutin content was found the highest level (53.8 mg/g dry wt.) at 30 DAS. In different plant parts harvested at 30 DAS, rutin content of leaf (range of 42.8-68.0, mean 57.0) was 5.3-fold higher than that of stem (range of 8.0-15.9, 10.8 mg/g dry wt.), regardless of cultivar. Significantly, rutin content (78.7) in the flower of Kitawase was 1.8 times higher than in the leaf and 9.8 times in the stem. Especially, chlorogenic acid content (14.6 mg/g dry wt.) in the flower of Kitawase was 63-fold higher than in the leaf, and 20-fold in the stem.

• KSBB Journal
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• 제15권3호
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• pp.323-328
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• 2000

한국산 장뇌삼과 재배인삼, 그리고 중국산 장뇌삼으로부터 유 리 아미노산을 추출하여 HPLC를 이용하여 14가지의 유리 아미 노산을 분석하였다. 전체적인 총 유리 아미노산의 크기를 비교 해 보면, P>M>KM4>CMl>KM7>Y>S>K>KM5>J>U>CM2>H> K KM2>KM1>KM6 순이었다 한국산 재배인삼 P의 아미노산 총 함량은 12.46 mg/g으로 가장 높았고, KM6가 6.86 mg/g으로 가 장 낮았다. 유리 아미노산 중에서 Arg은 Y에서 8.77 mg/g 함량 을 보였다. 총 유리 아미노산 중 Arg의 함량 비율은 KM2에서 7 77.78%로 가장 높게 나타났으며, 산지를 고려하지 않고 보면, 장뇌삼이 재배인삼과 구별되는 특정은 없었다. 한국산 장뇌삼은 H His, Met에서 한국산 재배인삼은 Thr이 특이적으로 다른 시료들 에 비해 다량 함유되어 았었고, 중국산 장뇌삼은 Lys만이 높은 함량 수치를 보였다. 특히 Gly은 한국산 시료에서 중국산 시료 에 비해 특이적으로 많이 함유하고 있었고, Lys은 중국산 장뇌 삼에 다른 한국산 시료에 비해 대체적으로 많이 함유되어 았었다

• 한국식품조리과학회지
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• 제11권3호
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• pp.303-308
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• 1995

참죽나무(Cedrela sinensis A.Juss) 잎을 재료로 하여, 김치, 나물, 부각 등의 조리하였을 때 이 식물의 flavonoid 화합물인 quercitrin의 함량 변화를 HPLC에 의한 용매계 THF-dioxane-MeOH-HOAc-5% H/Sub 3/PO/Sub 4/H/Sub 2/O(145:125:50:20:2:658)에서 분석하였다. 참죽김치를 5$^{\circ}C$에서 저장하였을 때의 quercitrin 함량은 1일째 13.06%, 6일째는 10.99%, 10일째는 11.49%, 20일째는 7.21%이며, 김치를 2$0^{\circ}C$에 저장하였을 시는 저장 1일째 15.33%, 6일째 10.83%, 10일째 9.85%, 20일째 5.78% 함유하고 있다. 나물의 경우 주성분인 quercitrin의 함량은 13.06%, 부각은 5.03%이었다. 따라서 김치의 경우 저장 일수가 길어짐에 따라 quercitrin함량은 감소하는 경향을 나타내며, 나물의 경우에는 quercetrin함량의 큰 변화를 볼 수 없고, 부각으로 조리한 경우 quercitrin의 함량은 급격히 감소하였다.

• 한국미생물·생명공학회지
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• 제19권5호
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• pp.477-486
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• 1991

Aspergillus niger가 생산하는 $\alpha$-galactosidase의 효소학적 성질을 조사하기 위하여 시험균주를 밀기울 배양한 후 생성된 $\alpha$-galactosidase를 염석, 이온교환 크로마토그래피 및 겔 여과 등의 방법으로 정제한 후 정제효소의 효소학적 성질을 검토하였다. Asp. niger를 밀기울 배지에서 $30^{\circ}C$, 4일 배양했을 때 효소활성이 가장 높았으며 $\alpha$-galactosidase는 황산암모늄 염석, DEAE-cellulose 및 DEAE-Sephadex A-50 이온교환 크로마토그래피, sephadex G-150 겔 여과 등에 의하여 23.7배까지 정제되었으며 비활성이 1,229U/mg.protein, 수율 14이었고 HPLC와 PAGE에 의해 순도가 확인되었다.

• 대한본초학회지
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• 제28권3호
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• pp.39-44
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• 2013

Objectives : The purpose of this study was to identify active fraction and components from antiplatelet Ulmi cortex extract. Methods : The 70% ethanol extract of Ulmi cortex was subjected to column chromatography over D101 resin and eluted with an 20% (W1), 30% (W2), 40% (W3), 50%(W4), 70% (W5), and 100% ethanol (W6) to yield 6 fractions. W6 was further fractioned and its active components were purified using semi-preparative HPLC. The isolated compounds were identified by MS and NMR, and their contents were simultaneously analyzed using HPLC/UV. Antiplatelet aggregation activities of the fractions and the compounds were evaluated using rat platelet-rich plasma in presence of collagen ($5{\mu}g/ml$), arachidonic acid (0.05 U/ml), or thrombin ($100{\mu}M$). Results : Among six fractions, W3 prominently inhibited platelet aggregation. At the concentration of $200{\mu}g/ml$, W3 strongly inhibited arachidonic acid- and collagen-induced platelet aggregations by 78.2% and 65.9%, respectivley, and weakly inhibited thrombin-inducded platelet aggregation by 32.6%. Catechin, epicatehin, and catechin-7-O-${\beta}$-D-glucopyranoside were isolated from W3 and their contents were revealed to be 15.1%, 0.87%, and 0.32%. Catechin and epicatechin at the concentrations of $100{\mu}M$ strongly inhibited collagen-induced platelet aggregation by 79.9% and 86.6%, respectively, but weakly inhibited arachidonic acid- and thrombin-induced platelet aggregations. Conclusions : A main active principle of anitplatelet Ulmi Cortex extract is W3 fraction, of which main active component is catechin considering its antiplatelet activity and content.