• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.47 no.5
• /
• pp.522-526
• /
• 2014

Pyropia, economic red algae species, have been cultivated in Korea (referred to as 'gim'), Japan ('nori'), and China ('zicai') for over 300 years. Vegetable seaweed Pyropia species are sold in the public markets in various forms as commercial seafoods. In Korea, two kinds of Pyropia seafood made with species of Pyropia and Ulva (sea lettuce, referred to as 'parae') are also sold. These are referred to as 'parae-gim' (with Pyropia spp. and U. linza) and 'gamtaegim' (with Pyropia spp. and U. prolifera). There is currently no method for identifying the seaweed species that comprise Pyropia seafood products. Therefore, we developed novel molecular markers to identify Ulva species in commercial Pyropia seafoods. Based on rbcL molecular markers, we identified informative characteristics to discriminate U. linza and U. prolifera as seafood ingredients. Moreover, PCR with 3'-end mismatch primers successfully isolated the specific rbcL sequences of U. linza and U. prolifera from Pyropia seafoods. Therefore, our novel molecular markers will be useful for identifying the ingredient species of commercial seafoods.

• Journal of the Korean Chemical Society
• /
• v.40 no.1
• /
• pp.50-58
• /
• 1996

In the presence of metal ions(Co(Ⅱ), Ni(Ⅱ), Sr(Ⅱ), Cu(Ⅱ), Fe(Ⅲ), U(Ⅵ)), the sedimentation of humic acid was increased at constant pH with increasing metal concentration and the strength was increased in the following order: Sr < Ni < Co < Cu < Fe < U. At constant metal concentration, Cu(Ⅱ), Ni(Ⅱ), and Co(Ⅱ) ions caused an increase in sedimentation of humic acid as the solution pH increased, whereas Fe(Ⅲ) and U(Ⅵ) ions caused a decrease. Sr(Ⅱ) ions did not affect the sedimentation even with the variation of pH. The analysis of FT-IR spectra for the sediments prepared from the reaction between humic acid and metal ions showed that metal ions were bound to humic acid to form complexes, suggesting that the metal complexation plays an important role in the sedimentation of humic acid.

• The Journal of Korean Society of Virology
• /
• v.29 no.2
• /
• pp.129-136
• /
• 1999

Transient transfection assay has been done to evaluate whether the c-jun activation would be prerequisite to the induction of permissiveness against human cytomegalovirus using in vitro cell model in which U937 has been induced to express CD11b and CD14 to become potential monocyte/macrophage cells by TPA treatment. U937 cells were treated with $10\;{\mu}M$, $50\;{\mu}M$ or $100\;{\mu}M$ of TPA. The cell morphology change was observed and the expression of the CD11b and CD14 was confirmed by FACS. Differentiated cells were transfected with pJLuc reporter vector which contained the wild type murine c-jun promoter spanning the SP1, CTF, ATF/CREB and MEF-2 binding sites upstream of the firefly luciferase gene. After 48 hrs of transfection, the cells were infected with HCMV Towne strain and the luciferase activity was assessed at 1 hand 4 h pi. The transfection assay showed no activation of the c-jun promoter at 1 h pi, instead, it showed 2 times increase of the its activity at 4 h pi. There was no difference of the c-jun promoter activation between TPA treated and untreated U937 cells, implying that c-jun activation might not be prerequisite for allowing cells to be premissive to HCMV, although HCMV infection itself could activate c-jun promoter.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.38 no.12
• /
• pp.1379-1385
• /
• 2014

Even the rough prediction of the product test time before the lifetime test of mechanical component begins would be of use in estimating cost and deciding how to keep up with the test. The reliability predictions of mechanical components are difficult because failure or degradation mechanisms are complicated, and few plausible databases are available for lifetime prediction. Therefore, this study conducted lifetime predictions of elastomeric U seals that were respectively installed in a hydraulic actuator and a pneumatic actuator using lifetime models and a field database based on failure physics and an actual test database obtained from the NSWC handbook. To validate the results, the predicted failure rates were compared with the actual lifetime test results acquired in the lab durability tests. Finally, this study discussed an engineering procedure to determine the coefficients in the failure rate models and analyzed the sensitivity of each influential parameter on the seal lifetime.

• Korean Journal of Medicinal Crop Science
• /
• v.16 no.2
• /
• pp.74-78
• /
• 2008

Liquiritin in licorice (Glycyrrhiza uralensis Fisch) extract was treated with three different plant crude enzymes (Prunus dulcis enzyme; PDE, P. armeniaca enzyme; PAE and P. persica enzyme; PPE) for biotransformation. The resulting product of liquiritin was analyzed by TLC and HPLC. The ${\beta}glucosidase$ activities of crude enzymes were 259.6 U/g (PDE), 407.6 U/g (PAE) and 445.8 U/g (PPE), respectively. The liquiritin was converted to liquiritigenin after 12 hours of incubation with the crude enzymes. Liquiritigenin content reached its maximum level after the treatment with PPE at $37^{\circ}C$.

• Parasites, Hosts and Diseases
• /
• v.54 no.5
• /
• pp.685-691
• /
• 2016

Chewing lice (Phthiraptera) that parasitize the globally threatened swan goose Anser cygnoides have been long recognized since the early 19th century, but those records were probably biased towards sampling of captive or domestic geese due to the small population size and limited distribution of its wild hosts. To better understand the lice species parasitizing swan geese that are endemic to East Asia, we collected chewing lice from 14 wild geese caught at 3 lakes in northeastern Mongolia. The lice were morphologically identified as 16 Trinoton anserinum (Fabricius, 1805), 11 Ornithobius domesticus Arnold, 2005, and 1 Anaticola anseris (Linnaeus, 1758). These species are known from other geese and swans, but all of them were new to the swan goose. This result also indicates no overlap in lice species between older records and our findings from wild birds. Thus, ectoparasites collected from domestic or captive animals may provide biased information on the occurrence, prevalence, host selection, and host-ectoparasite interactions from those on wild hosts.

• 한국태양에너지학회:학술대회논문집
• /
• 2008.04a
• /
• pp.215-220
• /
• 2008

This study shows the results on thermal performance test with domestic solar collector for low-temperature applications using KS, then reveals the efficiency difference between KS and EN standard. Using the test results, this study presents the status of thermal performance with domestic solar collector including flat-plate, single evacuated, and double evacuated (with mirror or U-tube) solar collector.

• Proceedings of the Korean Society of Medical Physics Conference
• /
• 2002.09a
• /
• pp.211-213
• /
• 2002

The purpose of this work is acquiring some parameters of therapeutic heavy ion beams after penetrating a thick target. The experiments were performed using a pencil-like $\^$12/C beam of about 3 mm in diameter from NIRS-HIMAC, and the data were taken at several points of the target thickness for $\^$12/C beam of 290 MeV/u and 400 MeV/u. By the simultaneous measurements using some detectors, the atomic number of each fragment particle was identified, and the beam profile, the dose distribution and the LET spectrum for each element were derived.

• Proceedings of the Korean Magnestics Society Conference
• /
• 2008.12a
• /
• pp.42.2-42.2
• /
• 2008

• Korean Journal of Mathematics
• /
• v.16 no.3
• /
• pp.259-269
• /
• 2008

The non-existence and existence of the positive solution for the generalized cooperation biological model for two species of animals $${\Delta}u+u(a-bu+g(v))=0\;in\;{\Omega}\\{\Delta}v+v(d+h(u)-cv)=0\;in\;{\Omega}\\u=v=0\;on\;{\partial}{\Omega}$$ are investigated. The techniques used in this paper are elliptic theory, upper-lower solutions, maximum principles and spectrum estimates. The arguments also rely on some detailed properties for the solution of logistic equations.