• 한국광학회:학술대회논문집
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• 한국광학회 2009년도 창립 20주년기념 특별학술발표회
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• pp.90-91
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• 2009

• 한국수소및신에너지학회논문집
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• 제19권2호
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• pp.124-131
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• 2008

Crude cytoplasmic fraction of phototrophic purple sulfur bacterium, Thiocapsa roseopersicina NCIB 8347, were initially prepared and purified by sonication, ultracentrifugation, ammonium sulfate fractionation and heat-treatment and it has been previously reported. Using various applications of chromatography far the purification of membrane-bound and soluble hydrogenases from heat-treated enzyme fraction were studied at present report. When the heat-treated enzyme preparation was applied to the anion column chromatography using Q-sepharose, Fraction I and II, which were extracted with the KCl 0-0.5 M gradient, showed the specific evolution hydrogenase activity 3.86 and 2.27 U/mg-protein respectively. Specific hydrogenase activitys of Fraction I and II were further increased to 4.35 and 7.46 U/mg-protein for Fraction I and to 2.49 and 4.41 U/mg-protein fur Fraction II respectively, when hydrophobic interaction column, Phenyl superose, and anion exchange column, Mono-Q, were applied. Size exclusion chromatography using superdex 200 concentrated the hydrogenase Fraction I and II to 9.19 and 7.84 U/mg-protein respectively at the final step of purification.

• KSBB Journal
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• 제26권3호
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• pp.243-247
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• 2011

Using Bacillus subtilis spore display system, with cotG as an anchoring motif, levansucrase from Zymomonas mobilis, was displayed on the outer surface of Bacillus subtilis spore. Flow cytometry of DB104 (pSDJH-cotG-levU) spore, proved the surface localization of CotG-LevU fusion protein on the spore compared to that of DB104. Enzymatic activity of DB104 (pSDJH-cotG-levU) spore showed more than 1.5 times higher levansucrase specific activity compared to that of the host spore, which is a remarkable increase of enzymatic activity considering the existence of sacA (sucrase) and sacB (levansucrase) in the Bacillus subtilis chromosome. The spore integrity, revealed by sporulation frequency test after heat and lysozyme treatment of spore, did not changed at all in spite of the CotG-LevU fusion protein incorporation into the spore coat layer during spore formation process. These data prove again that Bacillus subtilis spore could be considered as good live immobilization vehicle for efficient bioconversion process.

• 대한약학회:학술대회논문집
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• 대한약학회 2000년도 춘계총회 및 학술대회
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• pp.159.1-159.1
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• 2000

• Clinical and Experimental Reproductive Medicine
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• 제44권4호
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• pp.207-213
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• 2017

Objective: This study investigated the prevalence of infections with human papillomavirus, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, and Mycoplasma genitalium in the semen of Korean infertile couples and their associations with sperm quality. Methods: Semen specimens were collected from 400 men who underwent a fertility evaluation. Infection with above five pathogens was assessed in each specimen. Sperm quality was compared in the pathogen-infected group and the non-infected group. Results: The infection rates of human papillomavirus, C. trachomatis, U. urealyticum, M. hominis, and M. genitalium in the study subjects were 1.57%, 0.79%, 16.80%, 4.46%, and 1.31%, respectively. The rate of morphological normality in the U. urealyticum-infected group was significantly lower than in those not infected with U. urealyticum. In a subgroup analysis of normozoospermic samples, the semen volume and the total sperm count in the pathogen-infected group were significantly lower than in the non-infected group. Conclusion: Our results suggest that infection with U. urealyticum alone and any of the five sexually transmitted infections are likely to affect sperm morphology and semen volume, respectively.

• 전자공학회논문지
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• 제50권12호
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• pp.117-128
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• 2013

u-안심서비스 시스템은 GPS가 내장된 이동통신 긴급호출단말기, 이동통신망, 안심서비스사업자, 중계시스템, CCTV관제센터를 운영하는 단체와 CCTV망을 통하여 연동하므로 긴급 호출 신호 발생 시 실시간으로 호출자의 위치를 추적하고, 또 해당위치에 있는 CCTV들을 관제하여 호출자를 추적하여, 구난에 필요한 다양한 정보들을 보호자, 경찰서, 소방서, 병원, 구호단체 및 지자체 등에 SMS나 유선으로 제공할 수 있다. 본 논문에서는 u-안심서비스 중계시스템의 프로토콜 표준화를 위해 새로운 프로토콜을 설계하고 구현하였다. 설계한 u-안심서비스용 프로토콜(Protocol)을 실제 3GPP 및 3GPP2 이동 통신망에 적용했을 때의 성능을 시뮬레이션을 통하여 검증하였다.

• 한국전자파학회논문지
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• 제12권1호
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• pp.27-36
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• 2001

In this paper, micromachined millimeter-wave cavity resonators ar presented. One-port and two-port cavity resonators at Ka-band are designed using 3D design software, HP $HFSS^{TM}$ ver. 5.5 Cavity resonators are fabricated on Si substrate, which is etched down for the cavity, bonded with a Quartz wafer in which metal patterns for the feeding line coupling slot are formed. One-port resonator shows the resonant frequency of 39.34 GHz, the return loss of 14.5 dB, and the loaded $Q(Q_{L})$ of 150. Two-port cavity resonator shows the resonant frequency of 39 GHz, the insertion and return losses of 4.6dB and 19,9dB, the loaded($Q_{L}$) and unloaded $Q(Q_{U})$) of 44.3 and 107, respectively.

• 수산경영론집
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• 제17권1호
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• pp.107-115
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• 1986

The United States is the world's leading importer of frozen processed groundfish products, with over two thirds of total world imports. Over 90% of the U.S. groundfish demand is met by inports, while about 2 million mt of groundfish are taken from U, S. waters by joint-venture and foreign fleets. The objective of this paper is to provide descriptive information concerning the U.S. groundfish market and the potential for groundfish resources off Alaska to become a major source of supply to this market. The size of the U.S. market, U.S. imports, trade policies, and catch from U.S, waters are discussed, and a comparison is made between the potential domestic catch of groundfish off Alaska and current U.S. groundfish consumption. The total optimum yield of 2.3 million mt for flounders, cod, and pollock is about four times the round weight equivalent of U.S. imports of these species in 1984.

• 대한견주관절학회:학술대회논문집
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• 대한견주관절학회 1999년도 학술대회
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• pp.57-57
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• 1999

• 지능정보연구
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• 제14권2호
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• pp.21-44
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• 2008

최근 유비쿼터스 컴퓨팅 환경에서 등장하고 있는 각종 서비스들은 공통적으로 상황인식 컴퓨팅(context-aware computing)의 속성을 갖고 있다. 사용자를 둘러싼 다양한 상황(context)을 인식하여 다른 곳으로 정보를 전송하거나 지능적인(intelligent) 서비스를 제공해주는 것을 유비쿼터스 컴퓨팅 서비스의 기본 속성이라고 할 때, 유비쿼터스 컴퓨팅 서비스는 웰빙케어(well-being care) 분야에도 적용될 수 있다. 본 연구는 상황인식 컴퓨팅의 속성을 스트레스 관리를 위한 새로운 서비스에 적용해 봄으로써, 스트레스 정도를 객관적으로 측정할 수 있고 스트레스를 줄여주는 개인화 된 항스트레스(personalized anti-stress) 서비스를 제공할 수 있는 방법을 고안하였다. 이를 위해 본 연구는 스트레스 지수 모델(stress index model)을 개발하고, 스트레스를 측정하여 그에 맞는 항스트레스 서비스를 제공할 수 있는 방법론적인 접근을 시도하였다. 또한, 데모시스템의 테스트를 통해 스트레스 지수 모델의 기술적 구현 가능성을 확인하였다. 뿐만 아니라, 이와 같은 방법들을 사업적 관점에서 실제 적용할 수 있는 방법의 하나로서, 스트레스 측정과 관리를 주 목적으로 하는 u-SMC(Stress Management Center)의 비즈니스 모델을 개발함으로써 스트레스 관리를 위한 유비쿼터스 컴퓨팅 서비스의 상용화 가능성도 살펴보았다.