• 디지털융복합연구
• /
• 제18권1호
• /
• pp.241-247
• /
• 2020

전자파 노출이 자발운동에 따른 뇌의 유전자 발현에 미치는 영향을 10 주간 4그룹 즉, 정상 그룹, 자발운동 그룹, 전자파 노출 그룹, 전자파 노출 및 자발운동 그룹으로 나누어 조사하였다. 선조체(striata)와 시상하부(hypothalamus)에서 RT-PCR을 수행하였으며, 타이로신수산화효소(TH), FoxO3a, AMPKα, mRNA 발현을 조사하였다. 선조체에서 TH mRNA 발현은 자발운동과 전자파 노출 조건에서 각각 감소하였고, 전자파 노출 및 자발운동 그룹에서 더 많이 감소되었다. 이 결과는 전자파 노출 및 자발운동 그룹에서의 운동량 감소가 선조체에서 도파민이 감소할 수 있음을 시사한다. 선조체에서 FoxO3a mRNA 발현은 자발운동 그룹에서 증가했지만, 전자파 노출 및 자발운동 그룹은 현저히 감소했다. 시상하부에서는 TH mRNA 유전자 발현은 전자파 노출을 받은 자발운동 그룹에서 감소가 유의했으며, FoxO3a mRNA는 발현의 현저한 증가가 있었다. 전자파가 기억력에 미치는 영향도 밝히기 위해 해마에서의 여러 단백질들의 발현을 추후 조사할 것이다.

• Archives of Pharmacal Research
• /
• 제17권4호
• /
• pp.269-272
• /
• 1994

The effect of the root of Coptis japonica (COPT), both dichloromethane soluble $(CH_2Cl_2)$ and insoluble $(H_2O)$ fractions, on catecholamine contents and tyrosine hydorxylase (TH) activity in PC12 cells was investigated. $(CH_2Cl_2){\;}and{\;}H_2O$ fractions showed 21 and 53% inhibitions on dopamine content, respectively, at a ocncentraction of 40 .mu.g/ml in medium : the $(H_2O)$ fraction proveided a grateer inhibitory effect. The TH activity was reduced by the treatment of COPT ($(H_2O)$ fraction). These results suggest that COPT has an inhibitory effect on the catecholamine biosynthesis by the reduction of TH activity in PC12 cells.

• 동의신경정신과학회지
• /
• 제14권2호
• /
• pp.61-78
• /
• 2003

Objective : This study was designed to assess the protective effects of Chengwhabosimtang on the animal model of depression, chronic mild stress(CMS). Method : Male Sprague-Dawley rats were used for this experiment. The subjects were divided into 3 groups ( 1. CMS-drug: Chengwhabosimtang administered during CMS treatment, 2. CMS-vehicle: water administered, 3. normal ). After 4 weeks of CMS treatment, they were executed Forced swimming test(FST) and Elevated plus maze. Tyrosine hydroxylase(TH) in ventral tegmental area(VTA) and c-Fos in paraventricular nucleus(PVN) were measured. Result : 1. In FST, CMS-drug group showed significantly decreased immobility behavior. 2. CMS-drug group showed no significantly lower TH level in VTA than CMS-vehicle group. 3. CMS-drug group showed significantly less c-Fos expressed cell bodies in PVN than CMS-vehicle group. 4. In Elevated plus maze, CMS-drug group showed no significantly anxiety. Conclusion : These results suggest that Chengwhabosimtang may have protective antidepressant effects in CMS model rats. And these effects could be explained by the elevated stress-copying behaviors which are related with PVN of hypothalamus and dopaminergic neurons in VTA.

• 대한수의학회지
• /
• 제37권3호
• /
• pp.489-497
• /
• 1997

The distributions characteristics of neurons displaying immunoreactivity to the catecholamine synthetic enzymes, tyrosine hydroxylase(TH), dopamine-${\beta}$-hydroxylase(DBH), and phenylethanolamine-N-methyltransferase(PNMT) were examined in the adjacent sections of the substantia nigra & ventral tegmentum of the Striped Field Mouse(Apodemus agrarius coreae). None of these cell groups displayed either DBH or PNMT immunoreactivity. Many TH-immunoreactive neurons were present in the substantia nigra & ventral tegmentum. The major dopaminergic cell(TH-positive, DBH- & PNMT-negative) group in the midbrain was present in the pars compacta of substantia nigra and adjacent ventral tegmentum. And smaller dopaminergic cell groups Were found in the pars reticulata of the substantia nigra and central liner nucleus.

• Journal of Microbiology and Biotechnology
• /
• 제21권7호
• /
• pp.757-765
• /
• 2011

Many studies have suggested that the behavioral and reinforcing effects of morphine are induced by hyperactivation of the mesolimbic dopaminergic system, which results in increases in locomotor activity, c-Fos expression in the nucleus accumbens (NAc), and tyrosine hydroxylase (TH) in the ventral tegmental area (VTA). In order to investigate the effect of wild ginseng (WG) on treating morphine addiction, we examined the behavioral sensitization of locomotor activity and c-Fos and TH expression in the rat brain using immunohistochemistry. Intraperitioneal injection of WG (100 and 200 mg/kg), 30 min before administration of a daily injection of morphine (40 mg/kg, s.c.), significantly inhibited morphine-induced increases in c-Fos expression in NAc and TH expression in VTA as well as in locomotor activity, as compared with Panax ginseng. It was demonstrated that the inhibitory effect of WG on the behavioral sensitization after repeated exposure to morphine was closely associated with the reduction of dopamine biosynthesis and postsynaptic neuronal activity. It suggests that WG extract may be effective for inhibiting the behavioral effects of morphine by possibly modulating the central dopaminergic system and that WG might be a useful resource to develop an agent for preventing and treating morphine addiction.

• The Korean Journal of Physiology and Pharmacology
• /
• 제10권6호
• /
• pp.309-315
• /
• 2006

The protective effects of baicalein, one of the flavonoids in Scutellaria baicalensis Georgi, were evaluated against 6-hydroxydopamine (6-OHDA)-induced neuronal damage in mice and cultured human neuroblastoma cells. Nigrostriatal damage was induced by stereotaxically injecting 6-OHDA into the right striatum. Baicalein was administered intraperitoneally 30 min before and 90 min after lesion induction. Animals received a further daily injection of baicalein for 3 consecutive days. Two weeks after 6-OHDA injection, contralateral rotational asymmetry was observed by apomorphine challenge in lesioned mice. Tyrosine hydroxylase (TH) immunohistochemistry revealed a significant loss of terminals in lesioned striatum and the reduction of the numbers of TH-positive cell in the ipsilateral substantia nigra (SN). In addition, the levels of dopamine (DA) and DA metabolites were reduced and lipid peroxidation was increased in lesioned striatum. However, baicalein treatment reduced apomorphine-induced rotational behavior in 6-OHDA-lesioned mice, and increased TH immunoreactivity in the striatum and SN, and DA levels in lesioned striatum. Lipid peroxidation induced by 6-OHDA was also inhibited by baicalein treatment. Furthermore, when SH-SY5Y human neuroblastoma cells were treated with baicalein, 6-OHDA-induced cytotoxicity and reactive oxygen species (ROS) production were significantly reduced. These results indicate that baicalein effectively protects 6-OHDA-induced neuronal damage through antioxidant action.

• Journal of Genetic Medicine
• /
• 제4권2호
• /
• pp.179-185
• /
• 2007

목 적:카테콜라민은 교감신경계에서 신경전달물질이며 스트레스자극에 의해 활성화된다. TH와 DBH는 카테콜라민 합성에 매우 중요한 효소이다. CRH는 스트레스 반응에서 방출되는 호르몬이다. 이번 연구의 목적은 부동스트레스가 흰쥐 뇌에서 TH, BDH와 CRH 유전자발현에 어떤 영향을 미치는가를 알아보기 위한 것이다. 방 법:2시간 동안 부동스트레스와 무처치 흰쥐의 뇌에서 TH, DBH와 CRH 유전자 발현량을 비교하였다. TH, DBH와 CRH 유전자 발현은 RT-PCR과 western blotting analysis에 의해 정량하였다. 결 과:부동스트레스 흰쥐 그룹의 뇌와 부신에서 TH와 DBH 유전자발현은 정상그룹보다도 약 2-3배 유의하게 증가하였으며, CRH유전자는 약 1.5배 유의하게 증가하였다. 결 론:이번 연구는 흰쥐의 뇌와 부신에서 TH, DBH와 CRH 유전자는 스트레스 자극에 의해 발현이 활성화됨을 확인할 수 있었다.

• 한국발생생물학회:학술대회논문집
• /
• 한국발생생물학회 2004년도 추계학술대회
• /
• pp.22-22
• /
• 2004

• 한국발생생물학회:학술대회논문집
• /
• 한국발생생물학회 2004년도 추계학술대회
• /
• pp.21-21
• /
• 2004

• The Korean Journal of Physiology and Pharmacology
• /
• 제8권5호
• /
• pp.245-251
• /
• 2004

The effects of fetal mesencephalic cell grafts on the restoration of nigrostriatal dopaminergic function were studied in the intrastriatal 6-hydroxydopamine-lesioned rats. Four weeks after lesioning, transplantation of ventral mesencephalic cells from embryonic day 14 fetuses showed the number of tyrosine hydroxylase (TH) positive cells and fiber outgrowth in the grafted striatum, and significantly ameliorated symptomatic motor behavior of the animals, as determined by apomorphine-induced rotation. Furthermore, in substantia nigra pars compacta (SNc), the numbers of TH + cells and fibers were markedly restored. Dopamine content of ipsilateral SNc was close to that of contralateral SNc $(91.9{\pm}9.8%)$ in the transplanted animals, while the ratio was approximately 32% in sham-grafted animals. These results indicate that grafted cells restored the activity for the dopaminergic neurons located in SNc, although they were transplanted into striatum. In addition, we showed that the implanted fetal cells expressed high level of glial cell line-derived neurotrophic factor (GDNF), suggesting that the transplanted fetal cells might serve as a dopamine producer and a reservoir of neurotrophic factors. These results may be helpful in consideration of the therapeutic transplantation at early stage of PD.