• Journal of the Korean Society for Nondestructive Testing
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• 제27권5호
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• pp.393-397
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• 2007

Eddy current standard including an artificial slot for the calibration of absolute type surface probe was fabricated. Developed eddy current standard has the electric conductivity and dimensions, and contains artificial slot as established in ASTM E 1629. The width and depth of artificial slot are 0.1 mm and 0.5 mm respectively. This slot was only possible to measure the depth on the two side edges, and impossible for the middle part with general measurement tools. The ultrasonic test method was applied for measuring depth of the middle part of the artificial slot in the standard. Using this method the dimension could be measured successfully with uncertainty about $15\;{\mu}m$. Calibration of eddy current standard for the absolute probe can be performed by this technique.

• Korean Journal of Pharmacognosy
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• 제41권4호
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• pp.303-307
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• 2010

To overcome the limit of morphological method for classification of herbal drug, a novel method to discriminate its origin using pyrolysis mass spectrometry-multivariate analysis was developed. This method was applied successfully to Scutellaria baicalensis Georgi, one of the most popular herbal drug in oriental countries. The ethylacetate soluble fractions were prepared by sonication from pulverized roots of S. baicalensis which were collected from various regions including Korea and China, and subjected to direct insertion probe (DIP) mass spectrometry to achieve mass spectra of pyrolizates of extracts. The probe temperature was elevated from $30^{\circ}C$ to $320^{\circ}C$ at increasing rate $64^{\circ}C/min$, and the average mass spectrum calculated from total ion chromatography (TIC) was obtained. The relative peak intensities versus m/z were subjected to SAS program, and the training set (9 from Korea origin and 22 from China origin) was clustered two groups as its origin. In the test set, 11 samples among total 13 test sample were successfully classified according to their origin by developed method with accuracy of 85%.

• Fisheries and Aquatic Sciences
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• 제3권1호
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• pp.64-70
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• 2000

DNA probes for the l6S rRNA have been designed for the detection of Edwardsiella tarda. In order to accomplish this purpose, the l6S rRNA gene from E. tarda has been cloned and sequenced. Two highly feasible oligonucleotide probe sites have been determined by the database analysis programs presented by PCGENE and BLAST. These two probes have been evaluated by slot blot hybridization analysis. Hetero- and homo-trimeric templates have been synthesized using these two probe sites. The templates have been further multimerized by PCR to generate between 150 and 300 bp long DIG-11-dUTP labeled probes. Unlike 3' end labeled oligonucleotide probes or templates, multimerized probes showed no cross­hybridization in the given experimental condition. Furthermore, a significant increase in sensitivity has been observed with these probes. This method, we presented here, may be useful for the designing of probes for the detection of other fish pathogenic microorganisms also.

• Journal of the Korean Society of Safety
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• 제5권3호
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• pp.8-26
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• 1990

It is important to have exact information about the contact pressure distribution in the design of connected parts of machines and structures. In previous works, stress analyses on a two body contact problem have been carried out in large numbers. Besides, the measurement of contact stress is important to confirm the adequateness of the theoretical analysis, to verify appropriateness of Hertzian contact theory and to know the practical pressure distribution, but an excellent measuring method con't be found at present. Therefore, a quantitative measurement of contact pressure by means of ultrasonic waves using a normal probe and an angle has been proposed to measure the contact pressure distribution between two rough flat surfaces. At first, in a new proposed calibration method, the relation between mean contact pressure and sound pressure of reflected waves is obtained by using calibration blocks with various surface roughnesses made of the same material as the rectangular section beams And then, this experimental results are compared with the analytical ones, and the utility of this method is discussed.

• Journal of the Korean Society for Nondestructive Testing
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• 제30권4호
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• pp.386-395
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• 2010

The steam generator management program(SGMP) has recently defined the procedures for the qualification of eddy current hardware and technique. These procedures provide two basic methods for qualification. The first way is to qualify the equipment or the probe by using the flaw mechanism and method of the pulled tubes from the heat exchangers or the artificial flawed tubes. The second way is to verify the equivalency with the characteristics of the qualified equipment or probe. In this case, the qualified equipment or probe may be modified to substitute or replace instruments or probes without re-qualification provided that the range of essential variables defined in the examination technique specification sheet are met. This study is to describe the result of the comparative performance evaluation of bobbin coil eddy current probes manufactured by KEPCO Research Institute and probes manufactured by a foreign manufacturer. As a result of this study, although there were minor differences between the two kinds of probes, it was evaluated that the two kinds of probes were almost identical in the significant performance characteristics described in the KEPCO Research Institute guideline.

• BMB Reports
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• 제39권3호
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• pp.247-252
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• 2006

A rapid method for the detection of Hepatitis E Virus (HEV) was developed by utilizing nano-gold labeled oligonucleotide probes, silver stain enhancement and the microarray technique. The 5'-end -$NH_2$ modified oligonucleotide probes were immobilized on the surface of the chip base as the capture probe. The detection probe was made of the 3'-end -SH modified oligonucleotide probe and nano-gold colloid. The optimal concentrations of these two probes were determined. To test the detection sensitivity and specificity of this technique, a conservative fragment of the virus RNA was amplified by the RT-PCR/PCR one step amplification. The cDNA was hybridized with the capture probes and the detection probes on microarray. The detection signal was amplified by silver stain enhancement and could be identified by naked eyes. 100 fM of amplicon could be detected out on the microarray. As the results, preparation of nano-gold was improved and faster. Development time also was shortened to 2 min. Thus, considering high efficiency, low cost, good specificity and high sensitivity, this technique is alternative for the detection of HEV.

• Korean Journal of Veterinary Pathology
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• 제5권2호
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• pp.57-62
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• 2001

Recently various molecular diagnostic techniques have been used to identify rabbit hemorrhagic disease virus (RHDV), a causative agent responsible for acute hepatitis and disseminated intravascular coagulation in rabbit. But they were hard to perform and time consuming. To detect RHDV in a rapid and easy way, we developed biotinylated oligonucleotide probe within ORF 1 region encoding the polyprotein of RHDV in formalin-fixed and paraffin-embedded tissues from various tissues of 20 rabbits naturally infected with RHDV, Our in situ hybridization (ISH) was quickly carried out within two hours by MicroProbe capillary action system. The ISH produced a positive reaction in liver, kidney and lung. In conclusion, ISH with a biotintlated oligonucleotide probe provided a useful diagnostic method for detecting RHDV.

• Progress in Superconductivity and Cryogenics
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• 제9권3호
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• pp.13-15
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• 2007

We employed home-made position marking module in the scanning Hall probe system. The module is composed of two coils of which gap, a, between wires in a coil is $500{\mu}m$. We appiled 10-35mA of current with 15Hz in the coils and recorded ac corresponding magnetic field signal with respect to measuring time while we measured DC field profiles produced due to superconducting film in a perpendicular magnetic field. We calibrate the position, x, of coils using the measuring time and location of the coils in the holder. The error range was about ${\pm}0.1mm$. We test the module as we applied current of 100A and filed of 1kG in the superconducting tape. It was confirmed that there was no interference between superconducting tape and marking coils.

• Journal of the Korean Society for Precision Engineering
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• 제19권10호
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• pp.99-106
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• 2002

This paper proposes an error compensation method that improves accuracy with geometry information of injection molding parts. Geometric information can give an improved accuracy in reverse engineering. Measuring data can not lead to get accurate geometric model, including errors of physical parts and measuring machines. Measuring data include errors which can be classified into two types. One is molding error in product, the other is measuring error. Measuring error includes optical error of laser scanner, deformation by probe forces of CMM and machine error. It is important to compensate these in reverse engineering. Least square method (LSM) provides the cloud data with a geometry compensation, improving accuracy of geometry. Also, the functional shape of a part and design concept can be reconstructed by error compensation using geometry information.

• Smart Structures and Systems
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• 제28권6호
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• pp.727-735
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• 2021

Recently, non-destructive health monitoring methods such as magnetic flux leakage (MFL) method, have become popular due to their advantages over destructive methods. Currently, numerical study on this field has been limited to simplified studies by only obtaining MFL instead of induced voltage inside coil sensor. In this study, it was proposed to perform a novel numerical simulation of MFL's coil sensor by considering vital parameters including specimen's motion with constant velocity and saturation status of specimen in time domain. A steel-rod specimen with two stepwise cross-sectional changes (i.e., 21% and 16%) was fabricated using low carbon steel. In order to evaluate the results of numerical simulation, an experimental test was also conducted using a magnetic probe, with same size specimen and test parameters, exclusively. According to comparative results of numerical simulation and experimental test, similar signal amplitude and signal pattern were observed. Thus, proposed numerical simulation method can be used as a reliable source to check efficiency of sensor probe when different size specimens with different defects should be inspected.