• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 1999.10a
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• pp.446-451
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• 1999

A study for the roundness of machining center is classified into two ways. one is the way that progresses the roundness amending the parameter of machining center based on the measured value after the measurement of the roundness of machining center by means of a existing measuring device, another is the way that measures the roundness by remodeling the existing measuring device. The former is studied by pack hei jae team in Seoul university, the latter is studied by TSUTSUMI. Especially TSUTSUMI measures the roundness according to circular compensation after the insertion of developed measuring desire using a rotary encoder to the spindle of machining tool. We study how regulation velocity occuring with circular motion of machining center table influences the roundness after measuring the roundness using Circular Test method by a 2 dimention probe and a standard discus in this experiment.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2000.04a
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• pp.75-81
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• 2000

This study for the roundness and perpendicularity of machining center is classified into two ways. One is the way that progresses the roundness amending the parameter of machining center based on the measured value after the measurement of the roundness of machaning center by means of a existing measuring device, another is the way that measures the roundness by remodeling the existing measuring device. the former is studied by pack hei jae team in Seoul university, the later is studied by TSUTSUMI. Especially TSUTSUMI measures the roundness according to circular compensation after the insertion of developed measuring device using a rotary encoder to the spindle of machining tool. we study how regulation velocity occuring with circular motion of machining center table influences the roundness after measuring the roundness using Circular Test method by a 2 dimention probe and a standard discus in this experiment.

• Journal of the Korean Society for Nondestructive Testing
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• v.2 no.1
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• pp.10-16
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• 1982

This study was carried out to measure the flaw height of welds in consideration of the effective probe angle in ultrasonic oblique detection. Specimens with inserted artificial flaws were made and flaw heights were estimated from detecting these specimens. Two different methods were applied to estimate flaw heights. From the result of the experiment, flaw height could be measured within the accuracy of 15% percent error and the difference between the probe distance method and beam path method is about 5% relatively small. It is considered that the results obtained this experimental study could be helpful informal ions for measuring flaw height.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.792-795
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• 2005

The guide-ways of precision machine tools are one of important element of machine tools. It has usually a pair of surfaces for constraint of one direction with bearing. In the case of precision machine tools, non-contact bearing such as hydrostatic bearing and aerostatic bearing is adopted usually. In this case, profiles of rails has effect on straightness and the clearance of bearing has effect on stiffness of guide way, which changes to higher if clearance changes to smaller. The clearance is varied along moving table according to relative distance of pair of rails. The relative distance of pair of rail can be divided by three properties. First and second properties are straightness of each pair of rail and bearing pad. And, third is parallelism about pair of rails and pairs of bearing pad. There are several methods for measuring straightness of each surface such as reversal method, sequential two point method, and way straightness. These straightness measuring methods are always acquiring deviation of profile from eliminating linear fitted inclined line and don't have the information of parallelism. Therefore, to get the small clearance for high stiffness, the straightness of rail and bearing pad and parallelism about pair of rails and pair of bearing pads are measured for correction such as regrinding, reassembling and lapping. In this research, new and easy method for measuring parallelism of pair of rails is suggested. Two displacement probe and sensor stage, which is carry on the displacement sensor, are needed. The simulation and experiment was accomplished about pair of horizontal guide way to confirm the measurement of parallelism. And, the third probe is added to measure the straightness of each rails by sequential two point method. From the estimation of combined these two methods, it is confirmed that the profiles of a pairs of rails can be measured.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.4
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• pp.496-507
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• 2020

Virus infections of apples result in lowered commercial qualities such as low sugar content, weakened tree vigor, and malformed fruits. An effective way to control viruses is to produce virus-free plants based on the development of an accurate and sensitive diagnostic method. In this study, real-time PCR assays based on SYBR Green I and TaqMan probes were developed for detecting ASGV, ASPV, and ApMV viruses. These methods can detect and quantify 10³ to 10¹¹ RNA copies/μL of each virus separately. Compared with methods with two different dyes, the SYBR Green I-based method was efficient for virus detection as well as for assay using the TaqMan probe. Field tests demonstrated that real-time PCR methods developed in this study were applicable to high-throughput diagnoses for virus research and plant quarantine.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2012.05a
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• pp.633-634
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• 2012

• Journal of ILASS-Korea
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• v.21 no.4
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• pp.184-194
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• 2016

Many industrial processes require reliable temperature measurements in harsh environments with high temperature, dust, humidity, and pressure. However, commercially-available conventional temperature measurement devices are not suitable for use in such conditions. This study thus proposes a reliable, durable two-color radiation thermometer (RT) for harsh environments that was developed by selecting the appropriate components, designing a suitable mechanical structure, and compensating environmental factors such as absorption by particles and gases. The two-color RT has a simple, compactly-designed probe with a well-structured data acquisition system combined with efficient LabVIEW-based code. As a result, the RT can measure the temperature in real time, ranging from 300 to $900^{\circ}C$ in extremely harsh environments, such as that above the burden zone of a blast furnace. The error in the temperature measurements taken with the proposed two-color RT compared to that obtained using K-type thermocouple readouts was within 6.1 to $1.4^{\circ}C$ at a temperature range from 200 to $700^{\circ}C$. The effects of absorption by gases including $CO_2$, CO and $H_2O$ and the scattering by fine particles were calculated to find the transmittance of the two wavelength bands of operation through the path between the measured burden surface and the two-color probe. This method is applied to determine the transmittance of the short and long wavelength bands to be 0.31 and 0.51, respectively. Accordingly, the signals that were measured were corrected, and the true burden surface temperature was calculated. The proposed two-color RT and the correction method can be applied to measure temperatures in harsh environments where light-absorbing gases and scattering particles exist and optical components can be contaminated.

• Journal of the Optical Society of Korea
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• v.20 no.1
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• pp.199-203
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• 2016

An enzyme-free optical method is proposed for estimating high concentrations of glucose in a glucose-lactose mixture, based on a predictive equation that takes advantage of the reflective optical power observed at two discrete wavelengths. Compared to the conventional absorption spectroscopy method based on Beer's Law, which is mainly valid for concentrations below hundreds of mg/dL, the proposed scheme, which relies on reflection signals, can be applied to measure higher glucose concentrations, of even several g/dL in a glucose-lactose mixture. Two probe wavelengths of 1160 and 1300 nm were selected to provide a linear relationship between the reflective power and pure glucose/lactose concentration, where the relevant linear coefficients were derived to complete the predictive equation. Glucose concentrations from 2 to 7 g/dL in a glucose-lactose mixture were efficiently estimated, using the established predictive equation based on monitored reflective powers. The standard error of prediction was 1.17 g/dL.

• Journal of Advanced Marine Engineering and Technology
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• v.19 no.4
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• pp.34-41
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• 1995

The structure of premixed tubulent flames in a constant-volume vessel was investigated using a schlieren method and microprobe method. The schlieren method was used to observe the flame structure qualitatively. The microprobe method, which detects a flamelet by detecting its flame potential signal, was used to investigate the deeper flame structure behind the flame front. The flame potential signal having one to six peaks was obtained in the case of turbulent flames, each of them being regarede as a flamelet existing in the flame zone. Based on this consideration, the flame propagation speed, the thickness of the flame zone, the number of flamelets and the separation distance between adjacent flamelets in the flame zone were measured. Moreover, the thickness of flamelet which could not be attempted in the conventional electrostatic probe method was also investigated. The experimental results of this work suggest the existence of "reactant islands" in the reaction zone, and show that the averaged number of flamelets increases with an increase in the turbulence intensity and/or a decrease in the Damkohler number. The mean thickness of flamelet in the case of turbulent flames was found to be about two times compared to laminar values.ar values.

• Journal of Microbiology and Biotechnology
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• v.19 no.7
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• pp.658-665
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• 2009

Quality control QC for spot-uniformity is a critical point in fabricating an oligonucleotide array, and quantification of targets is very important in array analysis. We developed two new types of QC probes as a means of confirming the quality of the uniformity of attached probes and the quantification of targets. We compared the signal intensities and fluorescent images of the QC and target-specific probes of arrays containing only target-specific probes and those containing both QC and target-specific probes. In a comparison of quality control methods, it was found that the arrays containing QC probes could check spot-uniformity or spot defects during all processes of array fabrication, including after spotting, after washing, and after hybridization. In a comparison of quantification results, the array fabricated by the method using QC probes showed linear and regular results because it was possible to normalize variations in spot size and morphology and amount of attached probe. This method could avoid errors originating in probe concentration and spot morphology because it could be normalized by QC probes. There were significant differences in the signal intensities of all mixtures (P<0.05). This result indicates that the method using QC probes is more useful than the ordinary method for quantification of mixed target. In the quantification of mixed targets, this method could determine a range for mixed targets of various amounts. Our results suggest that methods using QC probes for array fabrication are very useful to the quality control of spots in the fabrication processes of quantitative oligonucleotide arrays.