• Journal of Microbiology and Biotechnology
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• v.18 no.6
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• pp.1064-1069
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• 2008

Levan fructotransferase (LFTase) preferentially catalyzes the transfructosylation reaction in addition to levan hydrolysis, whereas other levan-degrading enzymes hydrolyze levan into a levan-oligosaccharide and fructose. Based on sequence comparisons and enzymatic properties, the fructosyl transfer activity of LFTase is proposed to have evolved from levanase. In order to probe the residues that are critical to the intramolecular fructosyl transfer reaction of the Microbacterium sp. AL-210 LFTase, an error-prone PCR mutagenesis process was carried out, and the mutants that led to a shift in activity from transfructosylation towards hydrolysis of levan were screened by the DNS method. After two rounds of mutagenesis, TLC and HPLC analyses of the reaction products by the selected mutants revealed two major products; one is a di-D-fructose-2,6':6,2'-dianhydride (DFAIV) and the other is a levanbiose. The newly detected levanbiose corresponds to the reaction product from LFTase lacking transferring activity. Two mutants (2-F8 and 2-G9) showed a high yield of levanbiose (38-40%) compared with the wild-type enzyme, and thus behaved as levanases. Sequence analysis of the individual mutants responsible for the enhanced hydrolytic activity indicated that Asn-85 was highly involved in the transfructosylation activity of LFTase.

• Proceedings of the Korean Vacuum Society Conference
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• 2015.08a
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• pp.181.2-181.2
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• 2015

Sputtering is one of the most popular physical deposition methods due to their versatility and reproducibility. Synthesis of Cr thin films by DC magnetron sputtering using glancing angle deposition (GLAD) has been reported. Chromium thin films have been prepared at two different working pressure($2.0{\times}10-2$, 30, $3.3{\times}10-3torr$) on Si-wafer substrate using magnetron sputtering with glancing angle deposition (GLAD) technique. The thickness of Cr thin films on the substrate was adjusted about 1 mm. The electrical property was measured by four-point probe method. For the measurement of density in the films, an X-ray reflectivity (XRR) was carried out. The sheet resistance and column angle increased with the increase of glancing angle. However, nanohardness and density of Cr thin films decreased as the glancing angle increased. The measured density for the Cr thin films decreased from 6.1 to 3.8 g/cc as the glancing angle increased from $0^{\circ}$ to $90^{\circ}$ degree. The low density of Cr thin films is resulted from the isolated columnar structure of samples. The evolution of the isolated columnar structure was enhanced at the conditions of low sputter pressure and high glancing angle. This GLAD technique can be potentially applied to the synthesis of thin films requiring porous and uniform coating such as thin film catalysts or gas sensors.

• Journal of Advanced Marine Engineering and Technology
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• v.28 no.6
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• pp.922-930
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• 2004

An experimental investigation was performed to study on the generation of air bubble and air core with swirling flow in a horizontal cicular tube. To determine some characteristics of the flow, 2D PIV technique is employed for velocity measurement in water. The experimental rig is manufactured from an acryl tube. The test tube diameter of 80mm, and a length of 3000mm. The used algorithm is the gray leve cross-correlation method(Kimura et al. 1986). An Ar-ion laser is used and the light from the laser(500mW) passes through a probe to make two-dimensional light sheet. In order to make coded images of the tracer particles on one frame, an AOM(Acoustic-Optical Modulator) is used. The maximum axial velocities showed near the test tube wall at y/D =0.1 and y/D =0.9 along the test tube. The higher Reynolds number increase, the lower axial velocities are showed in the center of the test tube. The air bubbles are generated from Re =10,000 and developed into air core from the recirculating water pump rpm equal 30Hz. The pressure and temperature are measured across the test tube at X/D=3.33.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.3 no.2
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• pp.53-59
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• 2004

In this paper, we propose a new multi-purpose Scanning Force Microscope (SFM) system. The system can be used for nano/micro-scratching, in-process profile measurement, and observation of potential surface defects which occur during the scratching in air or liquid. Experimental results of nano/micro-scratching show that the smallest scratching depth can be controlled to be 10nm, which corresponds to the stability of the SFM system. Profile measurements of nano/micro-scratching surfaces have also been performed by the method of on-machine measurement and in-process measurement. Two measurement results were in good agreement with each other. The maximum difference was approximately 10 nm, which was mainly caused by the sampling repeatability error that influences the measurement accuracy Also, micro-defects on the micro-scratching surface were successfully detected by the SFM system. It was confirmed that the number of micro-defects increases when the surface is subjected to a cyclic bending load. The maximum depth was less than 100nm.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.3 no.3
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• pp.14-21
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• 2010

For the ubiquitous healthcare environment, real-time measurement of biomedical signals and accuracy of the measured biomedical information are very important. In addition, it is important to develop a healthcare device with low power In this paper, the synchronized pulse in a heartbeat was detected from the radial artery using the piezo film sensor, in order to eliminate inconvenience to wear a pulse detection finger probe. We can get a best output after applying the adaptive noise canceller using two piezo film sensor signals, pulse signal having motion artifacts and motion artifacts reference signal. To detect heartbeat, we use maximum point detection method from pulse removed motion artifacts.

• Transactions of the Korean Society of Mechanical Engineers
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• v.19 no.1
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• pp.219-230
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• 1995

The flow characteristics of a two-dimensional offset jet issuing parallel to a rough wall is experimentally investigated by using a split film probe with the modified Stock's calibration method. The mean velocity and turbulent stresses profiles in the up and down-stream locations of the wall-attachment regions are measured and compared with those of the smooth wall attaching offset jet cases. It is found that the wall-attachment region on the rough wall is wider than on the smooth wall for the same offset height and the jet speed. The position of the maximum velocity point is farther away from the wall than that for the smooth wall case because of the thick wall boundary layer established by the surface roughness. It is concluded that the roughness of the wall accelerates the relaxation process to a redeveloped plane wall jet and produces a quite different turbulent diffusion behavior especially near the wall from comparing with the smooth plane wall jet turbulence.

• Journal of Microbiology and Biotechnology
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• v.28 no.11
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• pp.1782-1790
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• 2018

Assessment of microorganism viability is useful in many industrial fields. A large number of methods associated with the use of fluorescent probes have been developed, including fluorimetry, fluorescence microscopy, and cytometry. In this study, a microvolume spectrofluorometer was used to measure the membrane potential variations of Escherichia coli. In order to estimate the sensitivity of the device, the membrane potential of E. coli was artificially disrupted using an ionophore agent: carbonyl cyanide 3-chlorophenylhydrazone. The membrane potential was evaluated using two ratiometric methods: a Rhodamine 123/4',6-diamidino-2-phenylindole combination and a JC-10 ratiometric probe. These methods were used to study the impact of freezing on E. coli, and were compared with the conventional enumeration method. The results showed that it was beneficial to use this compact, easy-to-use, and inexpensive spectrofluorometer to assess the viability of bacterial cells via their membrane potential.

• Journal of the Korea Convergence Society
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• v.7 no.5
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• pp.175-180
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• 2016

The demand for accurate freeform apsheric surface is increasing to satisfy the optical performance. In this paper, we develop the algorithm for opto-mechatronics convergence, that reconstruct the surface 3D profiles from the curvarure data along two orthogonal directions. A synthetic freeform surface with 8.4 m diameter was simulated for the testing. The simulation results show that the reconstruction error is 0.065 nm PV(Peak-to-valley) and 0.013 nm RMS(Root mean square) residual difference. Finally the sensitivity to noise is diagnosed for probe position error, the simulation results proving that the suggested method is robust to position error.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.273.1-273.1
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• 2014

Graphene, a two dimensional plane structure of $sp^2$ bonding, has been promised for a new material in many scientific fields such as physics, chemistry, and so on due to the unique properties. Chemical vapor deposition (CVD) method using transitional metals as a catalyst can synthesize large scale graphene with high quality and transfer on other substrates. However, it is difficult to control the number of graphene layers. Therefore, it is important to manipulate the number of graphene layers. In this work, homogeneous solid solution of Cu and Ni was used to control the number of graphene layers. Each films with different thickness ratio of Cu and Ni were deposited on $SiO_2/Si$ substrate. After annealing, it was confirmed that the thickness ratio accords with the composition ratio by X-ray diffraction (XRD). The synthesized graphene from CVD was analyzed via raman spectroscopy, UV-vis spectroscopy, and 4-point probe to evaluate the properties. Therefore, the number of graphene layers at the same growth condition was controlled, and the correlation between mole fraction of Ni and the number of graphene layers was investigated.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.6 no.2
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• pp.649-663
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• 2012

We propose a percolation based M2M networking architecture and its data transmission method. The proposed network architecture can be server-free and router-free, which allows us to operate routing efficiently with percolations based on six degrees of separation theory in small world network modeling. The data transmission can be divided into two phases: routing and data transmission phases. In the routing phase, probe packets will be transmitted and forwarded in the network thus multiple paths are selected and performed based on the constriction of the maximum hop number. In the second phase, the information will be encoded, say, with the fountain codes, and transmitted using the paths generated in the first phase. In such a way, an efficient routing and data transmission mechanism can be built, which allow us to construct a low-cost, flexible and ubiquitous network. Such a networking architecture and data transmission can be used in many M2M communications, such as the stub network of internet of things, and deep space networking, and so on.