• Korean Journal of Microbiology
• /
• v.55 no.3
• /
• pp.199-205
• /
• 2019

Yeast two-hybrid (Y2H) technique has been used to study protein-protein interactions, but its application particularly to a large-scale analysis of protein interaction networks, is limited by the fact that the technique is labor-intensive, based on scoring colonies on plate. Here, we develop a new reporter for the measurement of the protein-protein interactions by flow cytometry. The yeast harboring interacting proteins can also be enriched by fluorescence-activated cell sorting (FACS) or magnetic-activated cell sorting (MACS). When two interacting proteins are present in the same yeast cell, a reporter protein containing 10 tandem repeats of c-myc epitope becomes localized on the surface of the cell wall, without affecting cell growth. We successful measured the surface display of c-myc epitope upon interacting p53 with SV40 T antigen by flow cytometry. Thus, the newly developed Y2H assay based on the display of c-myc repeat on yeast cell wall could be used to the simultaneous analysis of multiple protein-protein interactions without laborious counting colonies on plate.

• Journal of the Korean Society for Industrial and Applied Mathematics
• /
• v.25 no.3
• /
• pp.93-106
• /
• 2021

In this paper, an efficient hybrid numerical method for solving two-asset option pricing problem is presented based on the Crank-Nicolson and the radial basis function methods. For this purpose, the two-asset Black-Scholes partial differential equation is considered. Also, the convergence of the proposed method are proved and implementation of the proposed hybrid method is specifically studied on Exchange and Call on maximum Rainbow options. In addition, this method is compared to the explicit finite difference method as the benchmark and the results show that the proposed method can achieve a noticeably higher accuracy than the benchmark method at a similar computational time. Furthermore, the stability of the proposed hybrid method is numerically proved by considering the effect of the time step size to the computational accuracy in solving these problems.

• International Journal of Aeronautical and Space Sciences
• /
• v.9 no.2
• /
• pp.79-86
• /
• 2008

High performance direct-iterative hybrid linear solver for large scale finite element problem is developed. Direct solution method is robust but difficult to parallelize, whereas iterative solution method is opposite for direct method. Therefore, combining two solution methods is desired to get both high performance parallel efficiency and numerical robustness for large scale structural analysis problems. Hybrid method mentioned in this paper is based on FETI-DP (Finite Element Tearing and Interconnecting-Dual Primal method) which has good parallel scalability and efficiency. It is suitable for fourth and second order finite element elliptic problems including structural analysis problems. We are using the hybrid concept of theses two solution method categories, combining the multifrontal solver into FETI-DP based iterative solver. Hybrid solver is implemented for our general structural analysis code, IPSAP.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.19 no.8
• /
• pp.862-877
• /
• 2008

In this paper, two types of wideband 3-dB ring hybrids are compared and discussed to show the ring hybrid with a set of coupled-line sections better. However, the better one still has a realization problem that perfect matching can be achieved only with -3 dB coupling power. To solve the problem, a set of coupled-line sections with two shorts is synthesized using one- and two-port equivalent circuits and design equations are derived to have perfect matching, regardless of the coupling power. Based on the design equations, a modified ${\Pi}-type$ of transmission-line equivalent circuit is newly suggested. It consists of coupled-line sections with two shorts and two open stubs and can be used to reduce a transmission-line section, especially when its electrical length is greater than ${\pi}$. Therefore, the $3\;{\lambda}/4$ transmission-line section of a conventional ring hybrid can be reduced to less than ${\pi}/2$. To verify the modified ${\Pi}-type$ of transmission- line equivalent circuit, two kinds of simulations are carried out; one is fixing the electrical length of the coupled-line sections and the other fixing its coupling coefficient. The simulation results show that the bandwidths of resulting small transmission lines are strongly dependent on the coupling power. Using modified and conventional ${\Pi}-types$ of transmission-line equivalent circuits, a small ring hybrid is built and named a compact wideband coupled-line ring hybrid, due to the fact that a set of coupled-line sections is included. One of compact ring hybrids is compared with a conventional ring hybrid and the compared results demonstrate that the bandwidth of a proposed compact ring hybrid is much wider, in spite of being more than three times smaller in size. To test the compact ring hybrids, a microstrip compact ring hybrid, whose total transmission-line length is $220^{\circ}$, is fabricated and measured. The measured power divisions($S_{21}$, $S_{41}$, $S_{23}$ and $S_{43}$) are -2.78 dB, -3.34 dB, -2.8 dB and -3.2 dB, respectively at a design center frequency of 2 GHz, matching and isolation less than -20 dB in more than 20 % fractional bandwidth.

• 제어로봇시스템학회:학술대회논문집
• /
• 1996.10b
• /
• pp.743-746
• /
• 1996

A hybrid control scheme to regulate the force and position by dual arms is proposed, where two arms are treated as one arm in a kinematic viewpoint. Our approach is different from other hybrid control approaches which consider robot dynamics, in the sense that we employ a purely kinematic based approach for hybrid control, with regard to the nature of position-controlled industrial robots. The proposed scheme is applied to sawing task. In the sawing task, the trajectory of the saw grasped by dual arms is planned in an offline fashion. When the trajectory of the saw is planned to follow a line in a horizontal plane, 3 position parameters are to be controlled(i.e, two translational positions and one rotational position). And a certain level of contact force has to be controlled along the vertical direction(i.e., minus z-direction) not to loose the contact with the object to be sawn. Typical feature of sawing task is that the contact position where the force control is to be performed is continuously changing. Therefore, the kinematic mapping between the force controlled position and the joint actuators has to be updated continuously. The effectiveness of the proposed control scheme is experimentally demonstrated. The proposed hybrid control scheme can be applied to arbitrary dual arm systems, regardless of their kinematic structure and the number of actuated joints.

• Journal of the Korean Mathematical Society
• /
• v.47 no.6
• /
• pp.1107-1122
• /
• 2010

In this paper, we shall use analytic methods to study the hybrid mean value involving the mixed two-term exponential sums C(m, n, r, $\chi$; q), and give several sharp asymptotic formulae.

• Journal of the Institute of Electronics Engineers of Korea TC
• /
• v.40 no.10
• /
• pp.16-23
• /
• 2003

In this paper, we propose a new optical hybrid switching system that takes advantage of both Optical Burst Switching (OBS) and Optical Circuit Switching (OCS) technologies. This system classifies incoming IP traffic flows into short-lived and long-lived flows for hybrid switching. For performance analysis, we model the system as a single server queue in a Markovian environment. The burst generation process is assumed to follow a two-state Markov Modulated Poisson Process (MMPP), and the service rate fluctuates based on the number of concurrent OCS sessions. Results of the mean delay and queue size for OBS bursts are derived.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.8 no.2
• /
• pp.129-133
• /
• 2004

The breeding work was initiated by utilizing two Japanese hybrids namely C135${\times}$N134 and N137${\times}$C146 along with two evolved Indian breeds, J14 and A24. The breed CSR16 which is characterized with marked larvae and white dumbbell cocoons was evolved from the Japanese hybrid C135${\times}$N134 by crossing with J14, while the breed CSR17 which is characterized with plain larvae and white oval cocoons was evolved from the Japanese hybrid N137${\times}$C146 by crossing with A24. The hybrid of these evolved breeds i.e., CSR16${\times}$CSR17 has shown superior over control hybrid $KA{\times}{NB_4}{D_2}$ and on par with the ruling hybrid of CSR2${\times}$CSR4. The hybrid CSR16${\times}$CSR17 was authorized during 1999 by Central Silk Board, Bangalore, Government of India for commercial exploitation during favourable months based on national level race authorization test.

• Tribology and Lubricants
• /
• v.27 no.2
• /
• pp.57-64
• /
• 2011

Hybrid air-foil magnetic bearing integrates two oil free bearing technologies synergetically to adopt the strengths of two bearings with minimizing their weaknesses. This paper presents bending mode vibration control of a flexible shaft supported by the hybrid air-foil magnetic bearing. An experiment set-up of a flexible shaft supported by the hybrid air-foil magnetic bearing is built. In order to verify the effectiveness of the hybrid bearing, unbalance responses of the flexible shaft supported by three different bearings: air-foil, magnetic and hybrid bearings are compared. Effect of load sharing between air-foil and magnetic bearings are investigated through changing the control gain and the rotor center position of magnetic bearing. The experimental results shows that the hybrid bearing can control the bending mode vibration of the flexible shaft effectively and an optimal performance can be achieved with an appropriate load sharing between the air-foil and the magnetic bearings.

• Proceedings of the Korean Society of Propulsion Engineers Conference
• /
• 2017.05a
• /
• pp.686-690
• /
• 2017

In this study, we numerically investigate the detonation characteristics (detonation velocity and pressure) of a hybrid ethylene-air and RDX mixture using two-phase model. Compared with detonation of pure ethylene-air mixture, the detonation of the hybrid ethylene-air and RDX mixture has higher pressure and stronger impulse because the hybrid mixture has additional chemical heat release of RDX particles. To validate the numerical results using two-phase model, we compare the experimental data which show changes of detonation pressure and velocity according to concentration of RDX particles.