• 제목/요약/키워드: two-hybrid

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T-DMB 하이브리드 데이터 서비스 Part 2: 하이브리드 서비스 저작 프레임워크 (T-DMB Hybrid Data Service Part 2: Hybrid Service Authoring Framework)

  • 임영권;김규헌;정제창
    • 방송공학회논문지
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    • 제16권2호
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    • pp.360-371
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    • 2011
  • T-DMB 하이브리드 데이터 서비스는 서비스를 구성하는 장면 기술 정보와 객체 기술 정보를 방송망 이외의 전송 경로를 통해 분산 전송할 수 있도록 구성하는 하이브리드 BIFS 기술을 이용하여 기존 T-DMB 수신기와의 역호환성을 보장하면서 새로운 데이터 서비스를 제공한다. 본 논문에서는 하이브리드 BIFS 기술을 이용하여 분산 전송이 가능한 BIFS를 구성하기 위한 하이브리드 서비스 저작 프레임워크의 구현 결과와 이를 이용한 실험 결과를 소개한다. 하이브리드 서비스 저작 프레임워크는 서비스 생성 시스템, 서비스 관리 시스템, 콘텐츠 제공 시스템 등으로 구성되며, 통합된 하이브리드 서비스를 저작하는 것은 물론 이를 방송망으로 전송되는 데이터와 무선 통신망을 통해 전송되는 개인맞춤형 데이터로 분할하여 생성하고 관리하는 기능을 제공한다. 이 서비스 프레임워크를 통해 구현된 콘텐츠는 기존 수신기와의 역호환성을 보장하면서 새로운 개인맞춤형 데이터 서비스 구현이 가능함을 검증하였다.

Hybrid-Ceramic Crown과 금속 도재관의 파절강도 비교 (COMPARISON OF FRACTURE STRENGTH BETWEEN HYBRID-CERAMIC CROWN AND METAL-CERAMIC CROWN)

  • 구철회;양홍서
    • 대한치과보철학회지
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    • 제39권1호
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    • pp.14-24
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    • 2001
  • The purpose of this study was to compare the fracture strengths and the fracture patterns of several hybrid-ceramic crowns and metal-ceramic crown. Ten crowns were constructed for each group according to the manufacturer's instruction. Removable template of silicone rubber impression material was used for standardization of each crowns. Each crown was cemented on a metal die with hybrid glass ionomer cement. All crowns cemented were stored in distilled water, $36^{\circ}C$ for 24 hours prior to loading in an universal testing machine. The load was directed at 130 degrees the long axis of metal die. The fracture strengths were measured and the fracture patterns were observed. The following results were obtained from this study 1. The mean fracture strengths of $Artglass^{(R)}$, $Sculpture^{(R)}$ and $Targis^{(R)}$ were $57.5{\pm}9.5Kgf,\;62.7{\pm}12.2Kgf$ and $60.2{\pm}10.1Kgf$ respectively. There was no significant difference among each hybrid ceramic crown group. 2. The toad required to fracture hybrid-ceramic crowns was significantly smaller than metal-ceramic crowns($131.7{\pm}22.0Kgf$). 3. In the metal-ceramic crowns, labial porcelain detached partially from porcelain-metal junction of proximal side by load. 4. Hybrid-ceramic crowns showed a simple fracture pattern that fracture line began at the loading area and extended through proximal surface, perpendicular to the margin. The crown was separated into two parts of labial side and lingual side. Above results revealed that three kinds of the hybrid-ceramic crowns used in this study must have careful application in clinical use since the strength of hybrid-ceramic crown was lower(about 1/2) than that of metal-ceramic crown.

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하이브리드 플립드 러닝과 플립드 러닝의 학습 효과 비교 (Comparison of learning effects between hybrid flipped learning and flipped learning)

  • 최보람
    • 대한물리치료과학회지
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    • 제31권2호
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    • pp.90-104
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    • 2024
  • Background: Hybrid learning is an educational approach that combines the teaching methods of online and lecture-style classes to compensate for each method's strengths and weaknesses. Compared to lecture-style classes, flipped learning improves overall class satisfaction and self-directed learning but is associated with lower learning motivation. It is necessary to determine whether hybrid flipped learning can solve the learning motivation problem of flipped learning by incorporating flipped learning into hybrid learning. The purpose of this study is to compare the effects of hybrid flipped learning and flipped learning on students' learning ability. Design: Cross-sectional study Methods: For students in the Department of Physical Therapy, classes were conducted using both flipped learning and hybrid flipped learning. In both learning methods, students took online classes first and participated in them every week. Flipped learning classes was conducted offline at school every week, while hybrid flipped learning alternated between live classes on YouTube and offline classes at school every other week. Results: Hybrid flipped learning resulted in significantly lower learning satisfaction and course evaluation than flipped learning, with no significant difference in grades. Conclusion: Hybrid flipped learning was able to cope with the situation well with the non-face-to-face teaching method caused by COVID-19, but it was difficult to improve learning ability because there were restrictions on activities that could interact with students. Flipped learning is a smooth offline activity that enables two-way activities between professors and students to improve learning ability, but the effect of improving test scores is still unclear.

스팀분사를 고려한 SOFC/GT 하이브리드 시스템의 설계 성능 비교 분석 (Design Performance Analysis of Solid Oxide Fuel Cell / Gas Turbine Hybrid Systems Considering Steam Injection)

  • 박성구;김동섭
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2007년도 춘계학술대회B
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    • pp.3224-3229
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    • 2007
  • This study aims to analyse the influence of steam injection on the performance of hybrid systems combining a solid oxide fuel cell and a gas turbine. The steam is generated by recovering heat from the exhaust gas. Two system configurations, with difference being the operating pressure of the SOFC, are examined and effects of steam injection on performances of the two systems are compared. Two representative gas turbine pressure ratios are simulated and a wide range of both the fuel cell temperature and the turbine inlet temperature is examined. Without steam injection, the pressurized system generally exhibits better system efficiency than the ambient pressure system. Steam injection increases system power capacity for all design cases. However, its effect on system efficiency varies much depending on design conditions. The pressurized system hardly takes advantage of the steam injection in terms of the system efficiency. On the other hand, steam injection contributes to the efficiency improvement of the ambient pressure system in some design conditions. A higher pressure ratio provides a better chance of efficiency increase due to steam injection.

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승용차용 다중모드 동력 전달 시스템의 정상상태 성능분석 (Steady State Performance Analysis of the Multi-mode Power Transmission Systems Equipped on Passenger Car)

  • 임원식;박윤경;박성천
    • 한국생산제조학회지
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    • 제22권3호
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    • pp.364-371
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    • 2013
  • Because of the increases in international oil prices and the level of global warming, the automotive industry has much interest in developing green cars with high fuel efficiencies. In addition, researchers in Korea are actively responding to high oil prices and $CO_2$ emission regulations in many ways. One example is, the multi-mode hybrid system, which is being studied to improve its performance. Because a multi-mode hybrid system is able to overcome the weaknesses of a system that uses simple planetary gears, excellent fuel efficiency and driving performances are the key features of the system. This paper analyzes the driving performance of the power-train system of GM-2MT70, which consists of one engine, two electric motors, one simple planetary gear, one double planetary gear, two clutches, and two brakes. The driving performance of the system's steady state is analyzed using performance modeling. The dynamic performance is analyzed using Matlab Simulink.

실시간 하이브리드 진동대 실험법에 의한 양방향 TLMD의 풍응답 제어성능평가 (Wind Response Control Performance of a Two-way Tuned Liquid Mass Damper Using Real-Time Hybrid Shaking Table Testing Method)

  • 허재성;이성경;이상현;박은천;김홍진;조봉호;조지성;김동영;민경원
    • 한국소음진동공학회:학술대회논문집
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    • 한국소음진동공학회 2007년도 추계학술대회논문집
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    • pp.189-194
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    • 2007
  • An experimental real-time hybrid method, which implements the wind response control of a building structure with only a two-way TLMD, is proposed and verified through a shaking table test. The building structure is divided into the upper experimental TLMD and the lower numerical structural part. The shaking table vibrates the TLMD with the response calculated from the numerical substructure, which is subjected to the excitations of the measured interface control force at its top story and an wind-load input at its base. The results show that the conventional method can be replaced by the proposed methodology with a simple installation and accuracy for evaluating the control performance of a TLMD.

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Identification of a Protein Interacting with Human Nebulin SH3 Domain by Yeast Two-hybrid Screening

  • Lee, Min-A;Kim, Ji-Hee;Min, Byung-In;Park, Soo-Ho;Ko, Han-Suk;Kim, Chong-Rak
    • 대한의생명과학회지
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    • 제7권2호
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    • pp.59-64
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    • 2001
  • Nebulin is an unusually large actin-binding protein specific to the skeletal muscle of vertebrates. The correlation of nebulin size with thin filament length have led to the suggestion that nebulin acts as a molecular ruler for the length of thin filaments. An SH3 domain occupies the C terminus of nebulin, in the sarcomeric Z-disk and is preceded by a 120-residue stretch containing multiple putative phosphorylation sites. SH3 domain mediates protein-protein interaction involved in the subcellular localization of proteins, cytoskeletal organization and signal transduction. However the binding partner and physiological role of nebulin SH3 domains remains unknown. Using the yeast two-hybrid system, we identified supervillin, an actin-binding protein, as a nebulin SH3 domain-interacting protein. The SH3 domain of nebulin binds to the sequence encoding amino acids 977 to 1335 of supervillin. But the sequence encoding amino acids 977 to 1335 displays weaker binding than the sequence encoding amino acids 977 to 1788.

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Mapping of the Interaction Domain of DNA Topoisomerase $II{\alpha}$ and $II{\beta}$ with Extracellular Signal-Regulated Kinase 2

  • Park, Gye-Hwa;Bae, Young-Seuk
    • BMB Reports
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    • 제34권1호
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    • pp.85-89
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    • 2001
  • Both topoisomerase $II{\alpha}$ and $II{\beta}$ east as phosphoproteins in the cells. Recently it was reported that DNA topoisomerase $II{\alpha}$ associates with and is phosphorylated by the extracellular signal-regulated kinase 2 (ERK2). Also, ERK2 stimulates the activity of topoisomerase II by a phosphorylation-independent manner [Shapiro et al., (1999) Mol. Cell. Biol. 19, 3551-3560]. In this study, a yeast two-hybrid system was used to investigate the binding site between topoisomerase $II{\alpha}$ or $II{\beta}$ and ERK2. The two-hybrid test clearly showed that topoisomerase $II{\beta}$ residues 1099-1263, and topoisomerase $II{\alpha}$ residues 1078-1182, mediate the interaction with ERK2, and that the leucine zipper motifs of topoisomerase $II{\alpha}$ and $II{\beta}$ are not required for its physical binding to ERK2. Our results suggest that topoisomerase $II{\beta}$ residues 1099-1263, and topoisomerase $II{\alpha}$ residues 1078-1182, may be common binding sites for activator proteins.

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A hybrid MC-HS model for 3D analysis of tunnelling under piled structures

  • Zidan, Ahmed F.;Ramadan, Osman M.
    • Geomechanics and Engineering
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    • 제14권5호
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    • pp.479-489
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    • 2018
  • In this paper, a comparative study of the effects of soil modelling on the interaction between tunnelling in soft soil and adjacent piled structure is presented. Several three-dimensional finite element analyses are performed to study the deformation of pile caps and piles as well as tunnel internal forces during the construction of an underground tunnel. The soil is modelled by two material models: the simple, yet approximate Mohr Coulomb (MC) yield criterion; and the complex, but reasonable hardening soil (HS) model with hyperbolic relation between stress and strain. For the former model, two different values of the soil stiffness modulus ($E_{50}$ or $E_{ur}$) as well as two profiles of stiffness variation with depth (constant and linearly increasing) were used in attempts to improve its prediction. As these four attempts did not succeed, a hybrid representation in which the hardening soil is used for soil located at the highly-strained zones while the Mohr Coulomb model is utilized elsewhere was investigated. This hybrid representation, which is a compromise between rigorous and simple solutions yielded results that compare well with those of the hardening soil model. The compared results include pile cap movements, pile deformation, and tunnel internal forces. Problem symmetry is utilized and, therefore, one symmetric half of the soil medium, the tunnel boring machine, the face pressure, the final tunnel lining, the pile caps, and the piles are modelled in several construction phases.

Site-Directed Mutagenesis on Putative Macrolactone Ring Size Determinant in the Hybrid Pikromycin-Tylosin Polyketide Synthase

  • Jung, Won-Seok;Kim, Eung-Soo;Kang, Han-Young;Choi, Cha-Yong;Sherman, David-H.;Yoon, Yeo-Joon
    • Journal of Microbiology and Biotechnology
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    • 제13권5호
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    • pp.823-827
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    • 2003
  • Streptomyces venezuelae ATCC 15439 is notable in its ability to produce two distinct groups of macrolactones. It has been reported that the generation of two macrolactone structures results from alternative expression of pikromycin (Pik) polyketide synthase (PKS). It was previously reported that the hybrid pikromycin-tylosin PKS can also produce two different macrolactones but its mechanistic basis remains unclear. In order to address this question, a series of site-directed mutagenesis of tentative alternative ribosome binding site and translation start codons in tylGV were performed. The results suggest that macrolactone ring size is not determined by the alternative expression of TylGV but through other mechanism(s) involving direct interaction between the PikAIII and TE domain or skipping of the final chain elongation step. This provides new insight into the mechanism of macrolactone ring size determination in hybrid PKS as well as an opportunity to develop novel termination activities for combinatorial biosynthesis.