• Title/Summary/Keyword: two-hybrid

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Estimation for the Half Logistic Distribution Based on Double Hybrid Censored Samples

  • Kang, Suk-Bok;Cho, Young-Seuk;Han, Jun-Tae
    • Communications for Statistical Applications and Methods
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    • v.16 no.6
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    • pp.1055-1066
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    • 2009
  • Many articles have considered a hybrid censoring scheme, which is a mixture of Type-I and Type-II censoring schemes. We introduce a double hybrid censoring scheme and derive some approximate maximum likelihood estimators(AMLEs) of the scale parameter for the half logistic distribution under the proposed double hybrid censored samples. The scale parameter is estimated by approximate maximum likelihood estimation method using two different Taylor series expansion types. We also obtain the maximum likelihood estimator(MLE) and the least square estimator(LSE) of the scale parameter under the proposed double hybrid censored samples. We compare the proposed estimators in the sense of the mean squared error. The simulation procedure is repeated 10,000 times for the sample size n = 20(10)40 and various censored samples. The performances of the AMLEs and MLE are very similar in all aspects but the MLE and LSE have not a closed-form expression, some numerical method must be employed.

Enabling role of hybrid simulation across NEES in advancing earthquake engineering

  • Gomez, Daniel;Dyke, Shirley J.;Maghareh, Amin
    • Smart Structures and Systems
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    • v.15 no.3
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    • pp.913-929
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    • 2015
  • Hybrid simulation is increasingly being recognized as a powerful technique for laboratory testing. It offers the opportunity for global system evaluation of civil infrastructure systems subject to extreme dynamic loading, often with a significant reduction in time and cost. In this approach, a reference structure/system is partitioned into two or more substructures. The portion of the structural system designated as 'physical' or 'experimental' is tested in the laboratory, while other portions are replaced with a computational model. Many researchers have quite effectively used hybrid simulation (HS) and real-time hybrid simulation (RTHS) methods for examination and verification of existing and new design concepts and proposed structural systems or devices. This paper provides a detailed perspective of the enabling role that HS and RTHS methods have played in advancing the practice of earthquake engineering. Herein, our focus is on investigations related to earthquake engineering, those with CURATED data available in their entirety in the NEES Data Repository.

Estimation of the exponential distribution based on multiply Type I hybrid censored sample

  • Lee, Kyeongjun;Sun, Hokeun;Cho, Youngseuk
    • Journal of the Korean Data and Information Science Society
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    • v.25 no.3
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    • pp.633-641
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    • 2014
  • The exponential distibution is one of the most popular distributions in analyzing the lifetime data. In this paper, we propose multiply Type I hybrid censoring. And this paper presents the statistical inference on the scale parameter for the exponential distribution when samples are multiply Type I hybrid censoring. The scale parameter is estimated by approximate maximum likelihood estimation methods using two different Taylor series expansion types ($AMLE_I$, $AMLE_{II}$). We also obtain the maximum likelihood estimator (MLE) of the scale parameter ${\sigma}$ under the proposed multiply Type I hybrid censored samples. We compare the estimators in the sense of the root mean square error (RMSE). The simulation procedure is repeated 10,000 times for the sample size n=20 and 40 and various censored schemes. The $AMLE_{II}$ is better than $AMLE_I$ in the sense of the RMSE.

The β Subunit of Heterotrimeric G Protein Interacts Directly with Kinesin Heavy Chains, Kinesin-I (Kinesin-I의 kinesin heavy chains과 직접 결합하는 heterotrimeric G protein의 β subunit의 규명)

  • Seog, Dae-Hyun
    • Journal of Life Science
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    • v.20 no.8
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    • pp.1166-1172
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    • 2010
  • Kinesin-I exists as a tetramer of two heavy chains (KHCs, also called KIF5s), which contain the amino (N)-terminal motor domain and carboxyl (C)-terminal domain, as well as two light chains (KLCs), which bind to the KIF5s (KIF5A, KIF5B and KIF5C) stalk region. To identify the interaction proteins for KIF5A, yeast two-hybrid screening was performed and a specific interaction with the ${\beta}$ subunit of heterotrimeric G proteins ($G{\beta}$) was found. $G{\beta}$ bound to the amino acid residues between 808 and 935 of KIF5A and to other KIF5 members in the yeast two-hybrid assay. The WD40 repeat motif of $G{\beta}$ was essential for interaction with KIF5A. In addition, these proteins showed specific interactions in the glutathione S-transferase (GST) pull-down assay. An antibody to KIF5s specifically co-immunoprecipitated KIF5s associated with heterotrimeric G proteins from mouse brain extracts. These results suggest that kinesin-I motor protein transports heteroterimeric G protein attachment vesicles along microtubules in the cell.

A Study on ESS Optimal Operation Strategy Using Two Stage Hybrid Optimization (Two Stage Hybrid Optimization을 사용한 ESS 최적 운전 전략에 대한 연구)

  • Gong, Eun-Kyoung;Sohn, Jin-Man
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.67 no.7
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    • pp.833-839
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    • 2018
  • This paper presents an analysis and the methodology of optimal operation strategy of the ESS(Energy Storage System) for reduce electricity charges. Electricity charges consist of a basic charge based on the contract capacity and energy charge according to the power usage. In order to use electrical energy at minimal charge, these two factors are required to be reduced at the same time. QP(Quadratic Programming) is appropriate for minimization of the basic charge and LP(Linear Programmin) is adequate to minimize the energy charge. However, the integer variable have to be introduced for modelling of different charge and discharge efficiency of ESS PCS(Power Conversion System), where MILP(Mixed Integer Linear Programming) can be used. In this case, the extent to which the peak load savings is accomplished should be assumed before the energy charge is minimized. So, to minimize the electricity charge exactly, optimization is sequentially performed in this paper, so-called the Two Stage Hybird optimization, where the extent to which the peak load savings is firstly accomplished through optimization of basic charge and then the optimization of energy charge is performed with different charge and discharge efficiency of ESS PCS. Finally, the proposed method is analyzed quantitatively with other optimization methods.

Design Optimization of Single-Stage Launch Vehicle Using Hybrid Rocket Engine

  • Kanazaki, Masahiro;Ariyairt, Atthaphon;Yoda, Hideyuki;Ito, Kazuma;Chiba, Kazuhisa;Kitagawa, Koki;Shimada, Toru
    • International Journal of Aerospace System Engineering
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    • v.2 no.2
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    • pp.29-33
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    • 2015
  • The multidisciplinary design optimization (MDO) of a launch vehicle (LV) with a hybrid rocket engine (HRE) was carried out to investigate the ability of an HRE for a single-stage LV. The non-dominated sorting genetic algorithm-II (NSGA-II) was employed to solve two design problems. The design problems were formulated as two-objective cases involving maximization of the downrange distance over the target flight altitude and minimization of the gross weight, for two target altitudes: 50.0 km and 100.0 km. Each objective function was empirically estimated. Several non-dominated solutions were obtained using the NSGA-II for each design problem, and in each case, a trade-off was observed between the two objective functions. The results for the two design problem indicate that economical performance of the LV is limited with the HRE in terms of the maximum downrange distances achievable. The LV geometries determined from the non-dominated solutions were examined.

A Study on the Development of Photoelastic Experimental Hybrid Method for Color Isochromatics (칼라 등색선무늬용 광탄성실험 하이브릿법 개발에 관한연구)

  • Kwon, O-Sung;Hawong, Jai-Sug;Nam, Jeong-Hwan;Han, Song-Ling;Kwon, Gun;Liu, Yi
    • Proceedings of the KSME Conference
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    • 2007.05a
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    • pp.66-71
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    • 2007
  • Isochromatics obtained from photoelastic experiment shows the stress distributions of full field of a structure under a load. Therefore stress distributions of the structure can be read at a glace through isochromatics. Many experimental data are obtained from isochromatics. And then, the various photoelastic experimental hybrid methods have been developed. Until now, monochromatic light has been used for photoelastic experimental hybrid method. Therefore the photoelastic experimental hybrid method used until now is called the photoelastic experimental hybrid method for black and white isochromatics. When stresses are analyed by photoelastic experimental hybrid method, many experimental data are needed. Therefore some fringe orders of isochromatics are needed for the photoelastic experimental hybrid method for white and black isochromatics. Therefore in this paper, the photoelastic experimental hybrid method for color isochromatics is developed. In this case, two fringe orders are enough for the experimental data of photoelastic experimental hybrid method for color isochromatics. Applying the method to stress concentration problems, its validity is confirmed. In the precision, the photoelastic experimental hybrid method for color isochromatics is better than the photoelastic experimental hybrid method for white and black isochromatics when fringe orders of isochromatics are few. When fringe orders are few, the photoelastic experimental hybrid method for color isochromatics can be used to analyze stress through few fringe orders of isochromatics.

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Physical Properties of Aramid and Aramid/Nylon Hybrid ATY for Protective Garments according to the Dry and Wet Texturing Conditions (건·습 텍스쳐링 가공조건이 방호의류용 Aramid ATY와 Aramid/Nylon hybrid 사의 물성에 미치는 영향)

  • Park, Mi Ra;Kim, Hyun Ah;Kim, Seung Jin
    • Fashion & Textile Research Journal
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    • v.15 no.3
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    • pp.444-451
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    • 2013
  • This paper surveys the physical properties of aramid and aramid/nylon hybrid air-jet textured yarns(ATY) for protective garments according to wet and dry texturing conditions. Aramid and nylon filaments were used to make two kinds of para-aramid ATY and four kinds of aramid/nylon hybrid ATY with dry and wet treatments. The analyzed physical properties of six specimens (made on the ATY machine) are as follows. The tenacity and initial modulus of aramid and aramid/nylon hybrid ATY decreased with the wetting and breaking strain; however, the yarn linear density of aramid and hybrid ATY increased with wetting treatment. The dry and wet thermal shrinkage of the hybrid ATY increased with wetting. The stability of aramid and hybrid ATY also increased with wetting. The physical properties of core/effect type hybrid ATY showed significantly more change than the core type hybrid ATY and the physical properties of nylon/aramid core/effect hybrid ATY showed significantly more change than the of aramid/nylon core/effect hybrid ATY. A higher bulky and breaking strain of hybrid ATY require ATY processing conditions of nylon on the core part with wetting and aramid on the effect part. ATY processing conditions for nylon and aramid on the core part with wetting are required for a higher tenacity and modulus. ATY processing conditions of nylon and aramid on the core with no wetting are required for a low thermal shrinkage.

Breeding Hybrid Rice with Genes Resistant to Diseases and Insects Using Marker-Assisted Selection and Evaluation of Biological Assay

  • Kim, Me-Sun;Ouk, Sothea;Jung, Kuk-Hyun;Song, Yoohan;Le, Van Trang;Yang, Ju-Young;Cho, Yong-Gu
    • Plant Breeding and Biotechnology
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    • v.7 no.3
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    • pp.272-286
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    • 2019
  • Developing elite hybrid rice varieties is one important objective of rice breeding programs. Several genes related to male sterilities, restores, and pollinators have been identified through map-based gene cloning within natural variations of rice. These identified genes are good targets for introducing genetic traits in molecular breeding. This study was conducted to breed elite hybrid lines with major genes related to hybrid traits and disease/insect resistance in 240 genetic resources and F1 hybrid combinations of rice. Molecular markers were reset for three major hybrid genes (S5, Rf3, Rf4) and thirteen disease/insect resistant genes (rice bacterial blight resistance genes Xa3, Xa4, xa5, Xa7, xa13, Xa21; blast resistance genes Pita, Pib, Pi5, Pii; brown planthopper resistant genes Bph18(t) and tungro virus resistance gene tsv1). Genotypes were then analyzed using molecular marker-assisted selection (MAS). Biological assay was then performed at the Red River Delta region in Vietnam using eleven F1 hybrid combinations and two control vatieties. Results showed that nine F1 hybrid combinations were highly resistant to rice bacterial blight and blast. Finally, eight F1 hybrid rice varieties with resistance to disease/insect were selected from eleven F1 hybrid combinations. Their characteristics such as agricultural traits and yields were then investigated. These F1 hybrid rice varieties developed with major genes related to hybrid traits and disease/insect resistant genes could be useful for hybrid breeding programs to achieve high yield with biotic and abiotic resistance.

Evaluation of the Estrogenic Activity by Yeast Two-hybrid Assay and Enzyme-linked Immunosorbent Assay in Sewage Treatment Plant (하수처리장의 내분비계장애물질에 대한 Yeast Two-hybrid Assay와 Enzyme-linked Immunosorbent Assay에 의한 에스트로겐활성도 평가)

  • Lee, Byoung-Cheun;Ra, Jin-Sung;Kim, Sang-Don;Kawai, Hukiko;Lee, Chul-Hee
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.7
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    • pp.771-777
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    • 2007
  • Several endocrine disrupting chemicals(EDCs) were monitored to evaluate the estrogenic activities and the concentrations by yeast two-hybrid assay and enzyme-linked immunosorbent assay(ELISA) in sewage treatment plant(STP) which consist of industrial and domestic line. In the influent of domestic line, estrone, 17$\beta$-estradiol, 17$\alpha$-ethinylestradiol and alkylphenolethoxylate(APE) were detected up to 167.1, 39.7, 7.3 and 145.4 ng/L, respectively. The average removal efficiency of 17$\beta$-estradiol after the activated sludge process was 77.5% and further removed to 80.8% after the sand filtration-ozonation step. These results suggests that the activated sludge process has limited potential to remove the estrogenic activity effectively. The contributions of the estrogenic chemicals to the estrogenic activities were 70.7, 23.3, 3.7 and 2.3% for estrone, 17$\beta$-estradiol 17$\alpha$-ethinylestradiol and APE, respectively, in the domestic line effluents. Therefore, 17$\beta$-estradiol and estrone contributed most of the estrogenic activity in the domestic line effluents.