• Journal of Navigation and Port Research
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• v.27 no.5
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• pp.539-546
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• 2003

The numerical model of the flow analysis by finite element technique is described. The Galerkin method is employed for spatial discretization Two step explicit finite element scheme is used to discretize the time function, which has advantage in problems treating large numbers of elements and unsteady state. Two dimensional hydrodynamic model considering moving boundary condition is developed. Also it applied flow model which develop on flow portion of ideal fluid in the model flume and verified, and the results of this study confirm the efficiency of moving boundary treatment in Jeju harbor. The computed results have shown the good adaptability of moving boundary condition From these studies, it can be concluded that the present method is a useful and effective tool in tidal flow analysis.

• Journal of Radiation Industry
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• v.6 no.2
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• pp.129-138
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• 2012

Proteomic analysis was performed in order to identify proteomic changes by salt stress between the Japonica cv. Donganbyeo (WT) and two salt-tolerant (ST) mutant lines by using the SDS-PAGE and 2-DE. Two salt tolerant rice mutant lines, ST-87 and ST-301, were selected by in vitro mutagenesis with gamma-ray. Three-week-old seedlings were treated with 171 mM NaCl for 7 days. In the SDS-PAGE, three proteins with molecular weights of 27, 46 and 58 kDa were highly increased under salt treatment. Total proteins from shoots of both WT and ST-lines were separated by two-dimensional gel electrophoresis. In 2-DE, 201, 226, 217 and 213 protein spots were detected in the untreated-or treated-WT and untreated- or treated-ST-87, respectively. Of theses, 17 and 10 protein spots were up- and down-regulated under salt stress in the WT, respectively. While, 16 and 8 protein spots were up- and down-regulated under salt stress in the ST-87, respectively, compared with the untreated plants. High intensity or de novo synthesized proteins were analyzed by MALDI-TOF/MS analysis.

• Clinical and Experimental Reproductive Medicine
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• v.49 no.3
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• pp.175-184
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• 2022

Objective: The aim of this study was to evaluate the impacts of platelet-rich plasma (PRP) and conditioned medium (CM) derived from endometrial stromal cells on mouse preantral follicle culture in a two-dimensional system to produce competent mature oocytes for fertilization. Methods: In total, 240 preantral follicles were isolated from female mouse ovarian tissue and divided into four groups. The preantral follicles were isolated three times for each group and then cultured, respectively, in the presence of alpha minimum essential medium (control), PRP, CM, and PRP+CM. The in vitro growth, in vitro maturation, and cleavage percentage of the preantral follicles were investigated. Immunocytochemistry (IHC) was also conducted to monitor the meiotic progression of the oocytes. Additionally, the mRNA expression levels of the two folliculogenesis-related genes (Gdf9 and Bmp15) and two apoptosis-related genes (Bcl2 and Bax) were investigated using real-time polymerase chain reaction. Results: In the PRP, CM, and PRP+CM groups, the preantral follicle maturation (evaluated by identifying polar bodies) were greater than the control group. The cleavage rate in the CM, and PRP+CM groups were also greater than the control group. IHC analysis demonstrated that in each treatment group, meiotic spindle was normal. In the PRP+CM group, the gene expression levels of Bmp15, Gdf9, and Bcl2 were greater than in the other groups. The Bax gene was more strongly expressed in the PRP and control groups than in the other groups. Conclusion: Overall, the present study suggests that the combination of CM and PRP can effectively increase the growth and cleavage rate of mouse preantral follicles in vitro.

• Korean Journal of Acupuncture
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• v.39 no.4
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• pp.142-151
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• 2022

Objectives : Parkinson's disease (PD) is a neurodegenerative disorder threatening the quality of life and highly occurred in over 65 years old. Gastrodia elata Blume (GEB), a traditional medicine used for the treatment of headache and convulsion, has been reported to have neuroprotective effect. This study was designed to investigate the neuroprotective effect of GEB and the proteomic changes in the substantia nigra (SN) of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice. Methods : Male eleven-week-old C57BL/6 mice were intraperitoneally injected with 30 mg/kg of MPTP at 24-h intervals for 5 days. Two hours after the daily MPTP injection, the mice were orally administered 800 mg/kg of GEB extract, which continued for 7 days beyond the MPTP injections, for a total of 12 consecutive days. Two hours after the final GEB administration, the brain samples were collected, and dopaminergic neuronal death and proteomic changes in the SN were evaluated. Results : GEB prevented the MPTP-induced dopaminergic neuronal death and regulated the expression of 11 proteins including thimet oligopeptidase, T-complex protein 1, glycine tRNA ligase, and pyruvate kinase isozymes M1. Conclusions : GEB prevents MPTP-induced dopaminergic neuronal death by regulating the proteins in the SN.

• Restorative Dentistry and Endodontics
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• v.38 no.1
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• pp.2-10
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• 2013

A common failure in endodontic treatment of the permanent maxillary first molars is likely to be caused by an inability to locate, clean, and obturate the second mesiobuccal (MB) canals. Because of the importance of knowledge on these additional canals, there have been numerous studies which investigated the maxillary first molar MB root canal morphology using in vivo and laboratory methods. In this article, the protocols, advantages and disadvantages of various methodologies for in-depth study of maxillary first molar MB root canal morphology were discussed. Furthermore, newly identified configuration types for the establishment of new classification system were suggested based on two image reformatting techniques of micro-computed tomography, which can be useful as a further 'Gold Standard' method for in-depth morphological study of complex root canal systems.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.9
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• pp.1209-1217
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• 2002

The use of a two dimensional hierarchical elements are investigated for the incompressible flow computation. The construction of hierarchical elements are explained by both a geometric configuration and a determination of degrees of freedom. Also a systematic treatment of essential boundary values has been developed for the degrees of freedom corresponding to higher order terms. The numerical study for the poisson problem shows that the computation with hierarchical higher order elements can increase the convergence rate and accuracy of finite element solutions in more efficient manner than the use of standard first order element. for Stokes and Cavity flow cases, a mixed version of penalty function approach has been introduced in connection with the hierarchical elements. Solutions from hierarchical elements showed better resolutions with consistent trends in both mesh shapes and the order of elements.

• Korean Journal of Agricultural Science
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• v.45 no.3
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• pp.521-532
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• 2018

In recent decades, the dry stream phenomena of small and medium sized rivers have been attracting much attention as an important social problem. To prevent dry stream phenomena, it is necessary to build an infrastructure that manages rivers. To accurately determine the progress of dry stream phenomena, it is necessary to continuously measure the discharge and other hydrological factors for small and medium sized rivers. However, until now, the flow data for small and medium rivers in Korea has been insufficient. To overcome the lack of supporting data for supporting rational decision-making in policy and project implementation, a short- and long-term hydrological model was developed that takes into consideration hydrological changes such as the increase of the impervious area due to urban development and groundwater pumping, the construction of a large-scale sewage treatment plant, the maintenance of stream-oriented rivers, etc. In the developed model, the distributed grid is represented by three layers: Surface flow, interflow, and groundwater flow. The surface flow and intermediate flow flowed along the flow direction, and the groundwater flow was calculated by a two-dimensional groundwater analysis model such that the outflow occurred in all directions without a specific flow direction. The effects of land use and cover on evapotranspiration and infiltration and the effects of multiple landscapes can be simulated in the developed model.

• Journal of the Society of Naval Architects of Korea
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• v.47 no.3
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• pp.388-397
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• 2010

A two-dimensional time-domain, potential-theory-based fully nonlinear numerical wave tank (NWT) was developed by using boundary element method and the mixed Eulerian-Lagrangian (MEL) approach for free-surface node treatment. The NWT was applied to prediction of primary wave energy conversion efficiency of a bottom-mounted oscillating water column (OWC) wave power device. The nonlinear free-surface condition inside the chamber was specially devised to represent the pneumatic pressure due to airflow velocity and viscous energy loss at the chamber entrance due to wave column motion. The newly developed NWT technique was verified through comparison with given experimental results. The maximum energy extraction was estimated with various chamber-air duct volume ratios.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1995.05a
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• pp.220-223
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• 1995

Stable polyion-complexed polymeric monolayrers were prepared by spreading perfluoroalkyl monomaleatd copolymers. C$_2$F$\_$8/MA-VE$_2$ and C$_2$F$\_$8/Mv-VE$_3$ on aqueous poly(allylamine) subphase. The monolayer properties have been studied by the surface pressure-area($\pi$-A) isotherms. The C$_2$F$\_$8/MA-VE$_3$containing longer oligoethyleneglycol pendant showed more expaned monolayer phase than the C$_2$F$\_$8/MA-VE$_2$ The polyion-complexed monolayers were transferable on various substrates, and the resulting Langmuir-Blodgett(LB) films were characterized b ET-IR spectroscopy and scanning electron microscopy(SEM) Two-dimensional crosslinking to from a polymer network was achieved by amide formation through heat treatment under vacuum with concurrent removel of perfluoroalkyl tails. SEM observation of this film ona porous membrane filter showed that the four layer film was sufficiently stable to cover the filter pore size of 0.1$\mu\textrm{m}$. The C$_2$F$\_$8/MA-VE$_3$revealed better covering capability than the C$_2$F$\_$8/MA-VE$_2$Immersion of this film in water or in benzend did not cause any change in its appearance and in FT-IR spectra.

• Radiation Oncology Journal
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• v.10 no.1
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• pp.95-100
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• 1992

Since LINAC-based stereotactic radiosurgery uses multiple noncoplanar arcs, three-dimensional dose evaluation and many beam parameters, a lengthy computation time is required to optimize even the simplest case by a trial and error. The basic approach presented in this paper is to show promising methods using an experimental optimization and an analytic optimization The purpose of this paper is not to describe the detailed methods, but introduce briefly, proceeding research done currently or in near future. A more detailed description will be shown in ongoing published papers. Experimental optimization is based on two approaches. One is shaping the target volumes through the use of multiple isocenters determined from dose experience and testing. The other method is conformal therapy using a beam's eye view technique and field shaping. The analytic approach is to adapt computer-aided design optimization in finding optimum irradiation parameters automatically.