• Title/Summary/Keyword: two component system

Search Result 1,212, Processing Time 0.041 seconds

Identification of Two-Component Regulatory Genes Involved in o-Xylene Degradation by Rhodococcus sp. Strain DK17

  • Kim, Doc-Kyu;Chae Jong-Chan;Zylstra Gerben J.;Sohn Ho-Yong;Kwon, Gi-Seok;Kim, Eung-Bin
    • Journal of Microbiology
    • /
    • v.43 no.1
    • /
    • pp.49-53
    • /
    • 2005
  • Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.

Measurement of the Three-Dimensional Flow Fields of a Gun-Type Gas Burner Using Triple Hot-Wire Probe (3중 열선 프로브를 이용한 Gun식 가스버너의 3차원 유동장 측정)

  • Kim, J.K.;Jeong, K.J.
    • Journal of Power System Engineering
    • /
    • v.10 no.3
    • /
    • pp.23-31
    • /
    • 2006
  • Mean velocities and turbulent characteristics in the three-dimensional flow fields of a gun-type gas burner were measured by using triple hot-wire probe (T-probe) in order to compare them with the results already presented by X-type hot-wire probe (X-probe). Vectors obtained by the measurement of two kinds of probes in the horizontal plane and in the cross section respectively show more or less difference in magnitude each other, but comparatively similar shape in overall distribution. Axial mean velocity component along the centerline shows that the value by T-probe is about ten times smaller than that by X-probe above the range of X/R=3. Also, the axial component of turbulent intensity along the centerline appears the biggest difference between the two probes. Moreover, axial mean velocity component, axial turbulent intensity component and rotational along the Y-directional distance show a big difference between slits and swirl vanes. On the whole, the values by T-probe appear smaller than those by X-probe.

  • PDF

Study on a Scheme of Investment Considering Customer Interruption Cost in Power Distribution System (정전비용을 고려한 배전계통 설비의 투자 계획 수립 방안연구)

  • Chu, Cheol-Min;Kim, Jae-Chul;Lee, Tae-Hee;Moon, Jong-Fil
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
    • /
    • 2006.05a
    • /
    • pp.365-369
    • /
    • 2006
  • It is concentrated on a methodology to establish a scheme of investment on power distribution systems of components. This paper provides a methodology to estimate the scheme as using a customer interruption cost regarding reliability indices in power distribution systems. The proposed method basically uses the failure rate depending on time for explaining the deterioration of a component. Therefore, the theory of the sensitivity is used for deciding the precedence of the investment to consider an effect of each component's failure rate on the system reliability. After Estimating the sensitivity on component investment cost making incremental reliability level is produced by component's investment cost accumulated according to the precedence of the sensitivity. After that, the failure rate corresponding with reliability level on the curve of investment cost is used as producing the curve of customer interruption cost. Two curves have the crossing point that is proposed to acceptable reliability level for customer and utility. In this paper, the acceptable reliability level for customer with the utility is assessed to analyze customer interruption cost and sensitivity of reliability indices. In conclusion the result of investment based on this method is shown to the reliability level with two cost.

  • PDF

CompGenX: Component Code Generation System based on GenVoca and XML (CompGenX: GenVoca와 XML 기반의 컴포넌트 코드 생성 시스템)

  • Choi Seung-Hoon
    • Journal of Internet Computing and Services
    • /
    • v.4 no.3
    • /
    • pp.57-67
    • /
    • 2003
  • Software product lines are to attain the rapid development of qualify applications by concretizing the general components populated in software assets and assembling them according to the predefined architectures. For supporting the construction of the software product lines, this paper proposes a component code generation techniques based on GenVoca architecture and XML/XSLT technologies, In addition, CompGenX(Component Generator using XML), a component code generation system, is proposed on the basis of this techniques. By providing reconfigurability of component at the time of code generation, CompGenX allows the reusers to create the component source code that is appropriate to their purpose, In this system, the process of the component development is divided into two tasks which are the component family construction task and the component reuse task, For the component family construction, CompGenX provides the feature modeling tool for domain analysis and the domain architecture definition tool. Also, it provides the tool for building the component configuration know1edge specification and the code templates, For the component reuse task, it offers the component family search tool. the component customizing tool and the component code generator. Component code generation techniques and system in this paper should be applicable as basic technology to build the component-based software product lines.

  • PDF

Development of Ternary Inorganic Binder System for Manufacturing High-Functional Ceramic Molds and Core (고기능성 세라믹 주형 및 중자 제작을 위한 3원계 무기 바인더 시스템 개발)

  • Hye-Yeong Park;Geun-Ho Cho;Hyun-Hee Choi;Bong Gu Kim;Eun-Hee Kim;SeungCheol Yang;Yeon-Gil Jung
    • Korean Journal of Materials Research
    • /
    • v.32 no.12
    • /
    • pp.538-544
    • /
    • 2022
  • In existing ceramic mold manufacturing processes, inorganic binder systems (Si-Na, two-component system) are applied to ensure the effective firing strength of the ceramic mold and core. These inorganic binder systems makes it possible to manufacture a ceramic mold and core with high dimensional stability and effective strength. However, as in general sand casting processes, when molten metal is injected at room temperature, there is a limit to the production of thin or complex castings due to reduced fluidity caused by the rapid cooling of the molten metal. In addition, because sodium silicate generated through the vitrification reaction of the inorganic binder is converted into a liquid phase at a temperature of 1,000 ℃. or higher, it is somewhat difficult to manufacture parts through high-temperature casting. Therefore, in this study, a high-strength ceramic mold and core test piece with effective strength at high temperature was produced by applying a Si-Na-Ti three-component inorganic binder. The starting particles were coated with binary and ternary inorganic binders and mixed with an organic binder to prepare a molded body, and then heat-treated at 1,000/1,350/1,500 ℃ to prepare a fired body. In the sample where the two-component inorganic binder was applied, the glass was liquefied at a temperature of 1,000 ℃ or higher, and the strength decreased. However, the firing strength of the ceramic mold sample containing the three-component inorganic binder was improved, and it was confirmed that it was possible to manufacture a ceramic mold and core via high temperature casting.

Engineering of Recombinant Escherichia coli Towards Methanol Sensing Using Methylobacterium extroquens Two-component Systems

  • Selvamani, Vidhya;Ganesh, Irisappan;Chae, Sowon;Maruthamuthu, Murali kannan;Hong, Soon Ho
    • Microbiology and Biotechnology Letters
    • /
    • v.48 no.1
    • /
    • pp.24-31
    • /
    • 2020
  • Five genes (mxbDM, mxcQE and mxaB) are responsible for the transcription of methanol oxidation genes in Methylobacterium strains. Among these, MxbDM and MxcQE constitute the two-component system (TCS) regulating methanol metabolism. In this study, we integrated the methanol-sensing domain of MxbD and MxcQ with the EnvZ/OmpR from Escherichia coli. The domain-swapping strategy resulted in chimeric histidine kinases (HK's) MxbDZ and MxcQZ AM1 containing recombinant E. coli. Real-time quantitative PCR was used to monitor OmpC expression mediated by the chimeric HK and response regulator (RR) OmpR. Further, an ompC promoter based fluorescent biosensor for sensing methanol was developed. GFP fluorescence was studied both qualitatively and quantitatively in response to environmental methanol. GFP measurement also confirmed ompC expression. Maximum fluorescence was observed at 0.05% methanol and 0.01% methanol using MxbDZ and MxcQZ AM1, respectively. Thus the chimeric HK containing E. coli were found to be highly sensitive to methanol, resulting in a rapid response making them an ideal sensor.

A Study on the Characteristics of In-Cylinder Air Flow with 3-D LDV Measurement (3차원 LDV를 이용한 실린더내 공기 유동특성에 관한 연구)

  • Yoo, S.C.
    • Journal of ILASS-Korea
    • /
    • v.11 no.1
    • /
    • pp.39-47
    • /
    • 2006
  • In-cylinder flows in a motored 3.5L four-valve SI engine were investigated quantitatively using three-component LDV system, to determine how engine configuration affects the flow field. The purpose of this work was to develop quantitative methods which correlate in-cylinder flows to engine performance. For this study, two distinct intake/piston arrangements were used to examine the flow characteristics. Quantification of the flow field was done by calculating two major parameters which are believed to characterize adequately in-cylinder motion. These quantities were turbulent kinetic energy(TKE) and tumble ratio in each plane at each crank angle. The results showed that in-cylinder flow pattern is dominated by the intake effects and two counter rotating vortices, developed during the intake stroke, produced relatively low tumble ratio. Therefore, the applicability of these quantities should be carefully considered when evaluating characteristics resulting from the complex in-cylinder flow motions.

  • PDF

Optimal Designs for Constant Stress Partially Accelerated Life Tests under Type I Censoring

  • Moon, Gyoung-Ae
    • Journal of the Korean Data and Information Science Society
    • /
    • v.6 no.2
    • /
    • pp.77-83
    • /
    • 1995
  • The inferences on a series system under the usual condition using data from constant stress partially accelerated life tests and type I censoring is studied. Two optimal designs to determine the sample proportion allocated each stress level model are also presented, which minimize the sum of the generalized asymptotic variances of maximum likelihood estimators of the failure rate and the acceleration factors and the sum of the asymptotic variances of maximum likelihood estimators of the acceleration factors for each component. Each component of a system is assumed to follow an exponenial distribution.

  • PDF

Reasonable Load Characteristic Experiment for Component Load Modeling (개별 부하모델링을 위한 부하의 합리적인 특성실험)

  • Ji, Pyeong-Sik;Lee, Jong-Pil;Im, Jae-Yun;Chu, Jin-Bu;Kim, Jeong-Hun
    • The Transactions of the Korean Institute of Electrical Engineers A
    • /
    • v.51 no.2
    • /
    • pp.45-52
    • /
    • 2002
  • Load modeling is classified into two methods according to approaching method, so called the measurement and component-based method. The measurement method is to model the load characteristics measured directly at substations and feeders. But it is difficult to measure continuously load characteristics from naturally occurring. system variation. The component-based method consists of the fellowing process; component load modeling, composition rate estimation and aggregation of component loads, etc. In this paper, the characteristic experiment of component loads was performed to obtain data for the component load modeling as the component-based method. At first, representative component loads were selected by the proposed method considering the accuracy of load modeling and the performance possibility of component load experiment in the laboratory. Also an algorithm was Proposed to identify the reliability of data obtained from the component load characteristic experiments. In addition, the results were presented as the case studies.

Pridiction of Adsorption Equilibrium for Binary Gas Mixtures on Cation Exchanged Forms of ZSM-5

  • Going Yim;Chai Suck Yim
    • The Journal of Engineering Research
    • /
    • v.6 no.2
    • /
    • pp.85-98
    • /
    • 2004
  • The adsorption equilibrium data for the binary gas mixture system from the pure gas adsorption data of carbon dioxide and ethylene on ZSM-5 prepared were predicted. The binary gas mixture adsorption data have been examined against predicted values by two models-the vacancy solution model(VSM) and the statistical thermodynamic model(STM), using parameters obtained from the single component isotherm. The binary gas mixture data for the carbon dioxide-ethylene system were obtained for cation exchanged forms of ZSM-5 for the gas phase carbon dioxide mole fraction of 0.752 at $37^{\circ}C$ and 1 atm. The experimental adsorption phase diagrams were obtained for carbon dioxide-ethylene on sodium form ZSM-5 synthesized. The single component adsorption isotherms for carbon dioxide and ethylene were also obtained for this zeolite. The single component data were used to obtain parameters derived in two models. These parameters were, in turn, used to predict the binary mixture isotherms for this zeolite. Both the vacancy solution and the statistical thermodynamic models give satisfactory predictions of adsorption phase diagrams for the binary gas mixtures of carbon dioxide and ethylene on sodium exchanged ZSM-5. Also the correlation between the experimental data and the predicted values is generally in good agreement. The system appears to show ideal behavior with a relatively constant separation factor. The slight increase in adsorption capacity with an increase in ionic radius is due, in part, to the higher polarizability associated with larger cations.

  • PDF