• KSBB Journal
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• 제15권5호
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• pp.489-496
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• 2000

고농도 유전자 재조합 대장균을 이용하여 pyruvate dehy-drogenase complex-E2 특이성 인간 모노클론 항체의 Fab 부분을 효율적으로 생산하기 위해 회분식, 이단 연속식, 반 유 가식, two-stage cyclic fed-batch 동 여러 가지 배양 방법이 조사되었다. 먼저 플라스미드 안정성 문제를 극복하기 위해 growth stage와 production stage를 분리하는 two-phase 회분식 배양과 이단 연속식 시스템을 시도하였다 그 결과 two-phase 회분식 배양보다는 이단 연속식 배양에서의 세포농도와 항체 생산성이 우수하였다 또한 이단 연속식 배양에서의 세포 성 장과 항처l 생산성은 용존산소를 제어한 경우가 그렇지 않은 경우보다 월등하게 높았다. 그리고 plasmid 안정성에 있어서 는 실험기간 내에 거의 100%를 유지하여 높은 안정도를 보 여주었다. 유가식 공정에 적합한 공급 배지로 변형된 M9 배 지가 최적배지로 선정되었고 이 배지 중 최적의 CjN 비율을 조사한 결파 2:3으로 결정되었다. 반 유가식 시스템에서 constant feeding 전략을 사용할 경우 최적 공급속도는 $0.6g/\ell/hr$이었다. 또한 pulse에 의해 공급배지를 공급할 경우에는 총 공급 량이 같을 경우 소량으로 자주 공급해 주는 것이 공급배지를 한꺼번에 많은 양을 공급해주는 것 보다 바람직하였다. 여러 가지 feeding 전략을 조사해 본 결과 linear feeding 방법이 가장 효과적이었다. 하지만 linear feeding 방법마저도 고농도 세포배양에 한계가 있었기 때문에 pH-stat 방법을 이용한 two-stage cyclic fed-batch 시스템을 시도하여 $54 g/\ell$의 세포 를 얻을 수 있었다. 따라서 이 방법이 일단 생산성 향상을 위한 세포의 고농도 배양에는 조사한 여러 배양 시스템 중에 가장 효율적인 시스템임올 알 수 있었다 하지만 이 시스템 에서 포도당을 낮은 level로 유지할 수 있었으나, 초산의 과도한 축적으로 항체 생산성의 향상은 예상에 비해 크지 않았다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1997년도 한국자동제어학술회의논문집; 한국전력공사 서울연수원; 17-18 Oct. 1997
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• pp.305-308
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• 1997

The on-line calculation method is developed to obtain the temperature trajectory that brings the reactants to the desired state in batch styrene polymerization reactor. The temperature trajectory is obtained by applying the moments of the polymer concentration to the 2-step calculation method. The computer simulation is also carried out to verify the superiority of the on-line method to the off-line one. When a temperature disturbance of constant size is introduced, the off-line results shows considerable deviation from the target degree of polymerization. The on-line strategy set up a new trajectory to reach the desired state by using the current state of the reactor. Therefore, the on-line strategy deals with the changes of the system more adequately than the off-line strategy.

• 제어로봇시스템학회논문지
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• 제18권3호
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• pp.285-294
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• 2012

Using bearing measurement only, target motion state is not directly obtained so that TMA (Target Motion Analysis) is needed for this situation. TMA is a nonlinear estimation technique used in passive SONAR systems. Also it is the one of important techniques for underwater combat management systems. TMA can be divided to two parts: batch estimation and sequential estimation. It is preferable to use sequential estimation for reducing computational load as well as adaptively to target maneuvers, batch estimation is still required to attain target initial state vector for convergence of sequential estimation. Selection of batch time interval which depends on observability is critical in TMA performance. Batch estimation in general utilizes predetermined batch time interval. In this paper, we propose a new method called the BTIS (Batch Time Interval and Initial State Estimation). The proposed BTIS estimates target initial status and determines the batch time interval sequentially by using a bank of GMM-TS (Gaussian Mixture Measurement-Track Splitting) filters. The performance of the proposal method is verified by a Monte Carlo simulation study.

• 대한산업공학회지
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• 제21권3호
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• pp.345-356
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• 1995

The aim of this paper is to analyze the batch service queueing model M/M(a, b ; ${\mu}_k/1$) under general bulk service rule with mean service rate ${\mu}_k$ for a batch of k units, where $a{\leq}k{\leq}b$. This queueing model consists of the two-dimensional state space so that it is characterized by two-dimensional state Markov process. The steady-state solution and performane measure of this process are derived by using Matrix Geometric method. Meanwhile, a new approach is suggested to calculate the two-dimensional traffic density R which is used to obtain the steady-state solution. In addition, to determine the optimal service initiation threshold a, a decision model of this queueing system is developed evaluating cost of service per batch and cost of waiting per customer. In a job order production system, the decision-making procedure presented in this paper can be applicable to determining when production should be started.

• Journal of the Korean Wood Science and Technology
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• 제45권4호
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• pp.409-418
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• 2017

This work attempted to manufacture glued-laminated timber (Glulam) bonded with melamine-urea-formaldehyde (MUF) resin adhesives at various melamine contents from 20% to 50% under high frequency (HF) heating for a very short time. Two preparation methods were employed to prepare MUF resin adhesives with different melamine contents: one-batch method of synthesizing MUF resins in a single batch, and two-batch method of mixing urea-formaldehyde (UF) resin with melamine-formaldehyde (MF) resin that had been synthesized separately. As the melamine content increased, the gelation time and peak temperature of MUF resins decreased. The adhesion performance of plywood showed that the one-batch MUF resin adhesive with 50% melamine content only satisfied the standard requirement of water resistance. Thus, the one-batch MUF resin adhesive with 50% melamine content was applied for bonding wood lamina from four softwood species such as Japanese larch, Korean red pine, Korean pine and Japanese cedar to manufacture Glulam under HF heating. All Glulam samples bonded with the one-batch MUF resin adhesives with 50% melamine content except those from Korean Red Pine satisfied the requirement in water soaking or boiling water delamination test as an exterior grade Glulam. The presence of rosin in Korean Red Pine was believed to be responsible for its poor adhesion. These results showed that the one-batch MUF resin adhesives with 50% melamine content provided acceptable water resistance with exterior grade Glulam manufactured under HF heating.

• 한국가시화정보학회지
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• 제13권3호
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• pp.24-28
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• 2015

The temperature of the wafer batch in the furnace was calculated and its visualized temperature field was analyzed. The main heat transfer mechanisms from the heater wall to the wafers were radiation and conduction, and the finite difference method was used to analyze the complex heat transfer including those two mechanisms. The visualized temperature field shows that the direction of the heat flux in the wafer batch varies during the heating process, and the heat in the wafer batch diffuses faster by conduction within the wafer than by radiation between the wafers, in the condition of the constant temperature at the heater wall and cap.

• Journal of the Korean Data and Information Science Society
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• 제28권6호
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• pp.1245-1255
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• 2017

온라인 학습은 자료가 실시간으로 혹은 배치 단위로 축적되는 상황에서 주어진 목적함수의 해를 계산하는 방법을 말한다. 온라인 학습 알고리즘 중 배치를 이용한 확률적 경사 하강법 (stochastic gradient decent method)은 가장 많이 사용되는 방법 중 하나다. 이 방법은 구현이 쉬울 뿐만 아니라 자료가 동질적인 분포를 따른다는 가정 하에서 그 해의 성질이 잘 연구되어 있다. 하지만 자료에 특이값이 있거나 임의의 배치가 확률적으로 이질적 성질을 가질 때, 확률적 경사 하강법이 주는 해는 큰 편이를 가질 수 있다. 본 연구에서는 이러한 비정상 배치 (abnormal batch) 있는 자료 하에서 효과적으로 온라인 학습을 수행할 수 있는 수정된 경사 하강 알고리즘 (modified gradient decent algorithm)을 제안하고, 그 알고리즘을 통해 계산된 해의 수렴성을 밝혔다. 뿐만 아니라 간단한 모의실험을 통해 제안한 방법의 이론적 성질을 실증하였다.

• Journal of Microbiology and Biotechnology
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• 제12권2호
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• pp.273-278
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• 2002

Two-step fed-batch fermentations were carried out to overproduce Bacillus licheniformis maltogenic amylase (BLMA) in recombinant Escherichia coli. The first step was to increase the cell mass by controlling the feeding of a glucose solution, while the second step was designed to improve the amylase expression efficiency by supplementing organic nitrogen sources. The linear gradient feeding method was successfully adopted to maintain the glucose concentration below 0.2 g/l during the fed-batch mode, as effectively minimizing acetic acid formation. When the dissolved oxygen (DO) level became limiting, an accumulation of acetic acid and drastic decrease in specific BLMA productivity were observed. Glucose and organic nitrogen sources consisting of yeast extract and casein hydrolysate were simultaneously supplied in the pH-stat mode to further increase the specific BLMA expression efficiency. An organic nitrogen source consisting of 200 g/1 yeast extract and 100 g/1 casein hydrolysate was found to be the best among the various combinations tested. The feeding of an organic nitrogen source in the second-step fed-batch period was highly beneficial in enhancing the BLMA production. The optimized two-step fed-batch culture resulted in 78 g/l maximum dry cell mass and 443 U/ml maximum BLMA activity, corresponding to 1.5-fold increase in the dry cell mass and 3.7-fold enhancement in BLMA production, compared with the simple fed-batch fermentation.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1995년도 Proceedings of the Korea Automation Control Conference, 10th (KACC); Seoul, Korea; 23-25 Oct. 1995
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• pp.319-322
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• 1995

As a trend toward multi-product batch processes is increasing in Chemical Process Industry (CPI), multi-product batch scheduling has been actively studied. But the optimal production scheduling problems for multi-product batch processes are known as NP-complete. Recently Ku and Karimi [5] have studied Simulated Annealing(SA) and Jung et al.[6] have developed Modified Simulated Annealing (MSA) method which was composed of two stage search algorithms for scheduling of batch processes with UIS and NIS. Jung et al.[9] also have studied the Common Intermediate Storage(CIS) policy which have accepted as a high efficient intermediate storage policy. It can be also applied to pipeless mobile intermediate storage pacilities. In spite of these above researches, there have been no contribution of scheduling of CIS policy for chemical batch processes. In this paper, we have developed another MSA for scheduling chemical batch processes with searching the suitable control parameters for CIS policy and have tested the this algorithm with randomly generated various scheduling problems. From these tests, MSA is outperformed to general SA for CIS batch process system.

• 한국기계가공학회지
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• 제17권5호
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• pp.161-172
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• 2018

This paper proposed the robot vision control method to track a moving rigid body target using the vision system model that can actively control camera parameters even if the relative position between the camera and the robot and the focal length and posture of the camera change. The proposed robotic vision control scheme uses a batch method that uses all the vision data acquired from each moving point of the robot. To process all acquired data, this robot vision control scheme is divided into two cases. One is to give an equal weight for all acquired data, the other is to give weighting for the recent data acquired near the target. Finally, using the two proposed robot vision control schemes, experiments were performed to estimate the positions of a moving rigid body target whose spatial positions are unknown but only the vision data values are known. The efficiency of each control scheme is evaluated by comparing the accuracy through the experimental results of each control scheme.