• KSBB Journal
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• v.15 no.5
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• pp.489-496
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• 2000

Several culture systems including batch, two-stage CSTR, semi-fed batch, and two-stage cyclic fed-batch were investigated for the efficient production of the Fab fraction of PDC-E2 specific human monoclonal antibody using high cell density recombinant E. coli. A two-phase batch system and a two-stage continuous system were examined to overcome plasmid instability problems, by separating the growth and the production stages. The cell density and productivity of the two-stage continuous culture was better than that of the two-phase batch fermentation. In the two-stage continuous culture system with DO-stat, the cell growth and the productivity were superior to those of the system without the DO control. Also, almost total plasmid stability was maintained in the two-stage continuous culture system. Modified M9 medium was selected as an optimum feeding medium for the fed-batch process, and the optimum C/N ratio determined to be 2:3. The optimum feeding rate was $0.6g/\ell/hr$ for a constant feeding strategy in semi-fed batch system. When the feeding medium was fed by pulsing, it was observed that more frequent pulsing resulted in improved cell growth. The linear feeding method was the most efficient of the various feeding methods tested. Finally, high cell density culture using a two-stage cyclic fed batch system with pH-stat was tried because the linear feeding method showed limitations in terms of obtaining high cell densities, and a cell density of $54 g/\ell$ was achieved. It was concluded that the two-stage cyclic fed batch system was the most efficient system for high cell density culture of the systems tested. However, productivity improvements were lower than expected due to the extremely high accumulations of acetate, although the low levels of residual glucose were maintained.

• 제어로봇시스템학회:학술대회논문집
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• 1997.10a
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• pp.305-308
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• 1997

The on-line calculation method is developed to obtain the temperature trajectory that brings the reactants to the desired state in batch styrene polymerization reactor. The temperature trajectory is obtained by applying the moments of the polymer concentration to the 2-step calculation method. The computer simulation is also carried out to verify the superiority of the on-line method to the off-line one. When a temperature disturbance of constant size is introduced, the off-line results shows considerable deviation from the target degree of polymerization. The on-line strategy set up a new trajectory to reach the desired state by using the current state of the reactor. Therefore, the on-line strategy deals with the changes of the system more adequately than the off-line strategy.

• Journal of Institute of Control, Robotics and Systems
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• v.18 no.3
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• pp.285-294
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• 2012

Using bearing measurement only, target motion state is not directly obtained so that TMA (Target Motion Analysis) is needed for this situation. TMA is a nonlinear estimation technique used in passive SONAR systems. Also it is the one of important techniques for underwater combat management systems. TMA can be divided to two parts: batch estimation and sequential estimation. It is preferable to use sequential estimation for reducing computational load as well as adaptively to target maneuvers, batch estimation is still required to attain target initial state vector for convergence of sequential estimation. Selection of batch time interval which depends on observability is critical in TMA performance. Batch estimation in general utilizes predetermined batch time interval. In this paper, we propose a new method called the BTIS (Batch Time Interval and Initial State Estimation). The proposed BTIS estimates target initial status and determines the batch time interval sequentially by using a bank of GMM-TS (Gaussian Mixture Measurement-Track Splitting) filters. The performance of the proposal method is verified by a Monte Carlo simulation study.

• Journal of Korean Institute of Industrial Engineers
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• v.21 no.3
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• pp.345-356
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• 1995

The aim of this paper is to analyze the batch service queueing model M/M(a, b ; ${\mu}_k/1$) under general bulk service rule with mean service rate ${\mu}_k$ for a batch of k units, where $a{\leq}k{\leq}b$. This queueing model consists of the two-dimensional state space so that it is characterized by two-dimensional state Markov process. The steady-state solution and performane measure of this process are derived by using Matrix Geometric method. Meanwhile, a new approach is suggested to calculate the two-dimensional traffic density R which is used to obtain the steady-state solution. In addition, to determine the optimal service initiation threshold a, a decision model of this queueing system is developed evaluating cost of service per batch and cost of waiting per customer. In a job order production system, the decision-making procedure presented in this paper can be applicable to determining when production should be started.

• Journal of the Korean Wood Science and Technology
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• v.45 no.4
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• pp.409-418
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• 2017

This work attempted to manufacture glued-laminated timber (Glulam) bonded with melamine-urea-formaldehyde (MUF) resin adhesives at various melamine contents from 20% to 50% under high frequency (HF) heating for a very short time. Two preparation methods were employed to prepare MUF resin adhesives with different melamine contents: one-batch method of synthesizing MUF resins in a single batch, and two-batch method of mixing urea-formaldehyde (UF) resin with melamine-formaldehyde (MF) resin that had been synthesized separately. As the melamine content increased, the gelation time and peak temperature of MUF resins decreased. The adhesion performance of plywood showed that the one-batch MUF resin adhesive with 50% melamine content only satisfied the standard requirement of water resistance. Thus, the one-batch MUF resin adhesive with 50% melamine content was applied for bonding wood lamina from four softwood species such as Japanese larch, Korean red pine, Korean pine and Japanese cedar to manufacture Glulam under HF heating. All Glulam samples bonded with the one-batch MUF resin adhesives with 50% melamine content except those from Korean Red Pine satisfied the requirement in water soaking or boiling water delamination test as an exterior grade Glulam. The presence of rosin in Korean Red Pine was believed to be responsible for its poor adhesion. These results showed that the one-batch MUF resin adhesives with 50% melamine content provided acceptable water resistance with exterior grade Glulam manufactured under HF heating.

• Journal of the Korean Society of Visualization
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• v.13 no.3
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• pp.24-28
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• 2015

The temperature of the wafer batch in the furnace was calculated and its visualized temperature field was analyzed. The main heat transfer mechanisms from the heater wall to the wafers were radiation and conduction, and the finite difference method was used to analyze the complex heat transfer including those two mechanisms. The visualized temperature field shows that the direction of the heat flux in the wafer batch varies during the heating process, and the heat in the wafer batch diffuses faster by conduction within the wafer than by radiation between the wafers, in the condition of the constant temperature at the heater wall and cap.

• Journal of the Korean Data and Information Science Society
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• v.28 no.6
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• pp.1245-1255
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• 2017

The online learning is a process of obtaining the solution for a given objective function where the data is accumulated in real time or in batch units. The stochastic gradient descent method is one of the most widely used for the online learning. This method is not only easy to implement, but also has good properties of the solution under the assumption that the generating model of data is homogeneous. However, the stochastic gradient method could severely mislead the online-learning when the homogeneity is actually violated. We assume that there are two heterogeneous generating models in the observation, and propose the a new stochastic gradient method that mitigate the problem of the heterogeneous models. We introduce a robust mini-batch optimization method using statistical tests and investigate the convergence radius of the solution in the proposed method. Moreover, the theoretical results are confirmed by the numerical simulations.

• Journal of Microbiology and Biotechnology
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• v.12 no.2
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• pp.273-278
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• 2002

Two-step fed-batch fermentations were carried out to overproduce Bacillus licheniformis maltogenic amylase (BLMA) in recombinant Escherichia coli. The first step was to increase the cell mass by controlling the feeding of a glucose solution, while the second step was designed to improve the amylase expression efficiency by supplementing organic nitrogen sources. The linear gradient feeding method was successfully adopted to maintain the glucose concentration below 0.2 g/l during the fed-batch mode, as effectively minimizing acetic acid formation. When the dissolved oxygen (DO) level became limiting, an accumulation of acetic acid and drastic decrease in specific BLMA productivity were observed. Glucose and organic nitrogen sources consisting of yeast extract and casein hydrolysate were simultaneously supplied in the pH-stat mode to further increase the specific BLMA expression efficiency. An organic nitrogen source consisting of 200 g/1 yeast extract and 100 g/1 casein hydrolysate was found to be the best among the various combinations tested. The feeding of an organic nitrogen source in the second-step fed-batch period was highly beneficial in enhancing the BLMA production. The optimized two-step fed-batch culture resulted in 78 g/l maximum dry cell mass and 443 U/ml maximum BLMA activity, corresponding to 1.5-fold increase in the dry cell mass and 3.7-fold enhancement in BLMA production, compared with the simple fed-batch fermentation.

• 제어로봇시스템학회:학술대회논문집
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• 1995.10a
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• pp.319-322
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• 1995

As a trend toward multi-product batch processes is increasing in Chemical Process Industry (CPI), multi-product batch scheduling has been actively studied. But the optimal production scheduling problems for multi-product batch processes are known as NP-complete. Recently Ku and Karimi [5] have studied Simulated Annealing(SA) and Jung et al.[6] have developed Modified Simulated Annealing (MSA) method which was composed of two stage search algorithms for scheduling of batch processes with UIS and NIS. Jung et al.[9] also have studied the Common Intermediate Storage(CIS) policy which have accepted as a high efficient intermediate storage policy. It can be also applied to pipeless mobile intermediate storage pacilities. In spite of these above researches, there have been no contribution of scheduling of CIS policy for chemical batch processes. In this paper, we have developed another MSA for scheduling chemical batch processes with searching the suitable control parameters for CIS policy and have tested the this algorithm with randomly generated various scheduling problems. From these tests, MSA is outperformed to general SA for CIS batch process system.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.17 no.5
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• pp.161-172
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• 2018

This paper proposed the robot vision control method to track a moving rigid body target using the vision system model that can actively control camera parameters even if the relative position between the camera and the robot and the focal length and posture of the camera change. The proposed robotic vision control scheme uses a batch method that uses all the vision data acquired from each moving point of the robot. To process all acquired data, this robot vision control scheme is divided into two cases. One is to give an equal weight for all acquired data, the other is to give weighting for the recent data acquired near the target. Finally, using the two proposed robot vision control schemes, experiments were performed to estimate the positions of a moving rigid body target whose spatial positions are unknown but only the vision data values are known. The efficiency of each control scheme is evaluated by comparing the accuracy through the experimental results of each control scheme.