• 제목/요약/키워드: tweezers

검색결과 41건 처리시간 0.038초

Simultaneous Detection of Biomolecular Interactions and Surface Topography Using Photonic Force Microscopy

  • 허승진;김기범;조용훈
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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    • pp.402.1-402.1
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    • 2014
  • Photonic force microscopy (PFM) is an optical tweezers-based scanning probe microscopy, which measures the forces in the range of fN to pN. The low stiffness leads proper to measure single molecular interaction. We introduce a novel photonic force microscopy to stably map various chemical properties as well as topographic information, utilizing weak molecular bond between probe and object's surface. First, we installed stable optical tweezers instrument, where an IR laser with 1064 nm wavelength was used as trapping source to reduce damage to biological sample. To manipulate trapped material, electric driven two-axis mirrors were used for x, y directional probe scanning and a piezo stage for z directional probe scanning. For resolution test, probe scans with vertical direction repeatedly at the same lateral position, where the vertical resolution is ~25 nm. To obtain the topography of surface which is etched glass, trapped bead scans 3-dimensionally and measures the contact position in each cycle. To acquire the chemical mapping, we design the DNA oligonucleotide pairs combining as a zipping structure, where one is attached at the surface of bead and other is arranged on surface. We measured the rupture force of molecular bonding to investigate chemical properties on the surface with various loading rate. We expect this system can realize a high-resolution multi-functional imaging technique able to acquire topographic map of objects and to distinguish difference of chemical properties between these objects simultaneously.

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Growth of Budding Yeasts under Optical Trap

  • Im, Kang-Bin;Kim, Hyun-Ik;Kim, Soo-Ki;Kim, Chul-Geun;Oh, Cha-Hwan;Song, Seok-Ho;Kim, Pill-Soo
    • Molecular & Cellular Toxicology
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    • 제3권1호
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    • pp.19-22
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    • 2007
  • Optic tweezer is powerful tool to investigate biologic cells. Of eukaryotic cells, it was poorly documented regarding to optic trapping to manipulate yeasts. In preliminary experiment to explore yeast biology, interferometric optical tweezers was exploited to trap and manipulate budding yeasts. Successfully, several budding yeasts are trapped simultaneously. We found that the budding direction of the daughter cell was almost outward and the daughter cell surrounded by other yeasts grows slowly or fail to grow. Thus it was assumed that neighboring cells around budding yeast may be critical in budding and the growth of daughter cells. This is first report pertaining to the pattern of yeast budding under the optical trap when multiple yeasts were trapped.

Multiplexed single-molecule flow-stretching bead assay for DNA enzymology

  • Lee, Ryanggeun;Yang, Keunsang;Lee, Jong-Bong
    • BMB Reports
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    • 제52권10호
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    • pp.589-594
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    • 2019
  • Single-molecule techniques have been used successfully to visualize real-time enzymatic activities, revealing transient complex properties and heterogeneity of various biological events. Especially, conventional force spectroscopy including optical tweezers and magnetic tweezers has been widely used to monitor change in DNA length by enzymes with high spatiotemporal resolutions of ~nanometers and ~milliseconds. However, DNA metabolism results from coordination of a number of components during the processes, requiring efficient monitoring of a complex of proteins catalyzing DNA substrates. In this min-review, we will introduce a simple and multiplexed single-molecule assay to detect DNA substrates catalyzed by enzymes with high-throughput data collection. We conclude with a perspective of possible directions that enhance capability of the assay to reveal complex biological events with higher resolution.

광핀셋의 원리 및 응용기술

  • 김종호
    • 기계저널
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    • 제44권10호
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    • pp.62-69
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    • 2004
  • 이 글에서는 마이크로/나노 스케일의 생체분자를 제어하거나 생체 분자의 기계적 물성치 측정 그리고 더 나아가 생체분자 상호간 작용력을 측정할 수 있는 광핀셋(optical tweezers) 원리 및 응용 예를 소개한다.

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주사 레이저 광집게를 위한 압전 구동기 주파수 특성 분석과 주사 경로 추적 및 보상 (Analysis of Frequency Response of Piezo Stages and Scanning Path Monitoring/Compensation for Scanning Laser Optical Tweezers)

  • 황선욱;이송우;이용구
    • 한국광학회지
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    • 제19권2호
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    • pp.132-139
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    • 2008
  • 주사 레이저 광집게에서 대물렌즈에 의해 집속된 레이저 초점을 제어하기 위해 사용되는 고속 구동기는 입력 신호의 주파수가 증가함에 따라 출력이 감소한다. 이러한 입출력의 괴리는 CCD 카메라를 통해 관찰이 어려우며 사용자는 희미하게 보이는 레이저 주사 형상을 보고 물체를 조작하여 물체를 포획할 수 없거나 포획하더라도 안정된 제어를 할 수 없다. 본 연구에서는 이러한 문제를 해결하기 위해 사용된 고속 구동기의 주파수 특성을 분석하고, 이를 바탕으로 입력 주파수에 따른 실제 주사 경로를 측정하여 시각화해주는 방법과 입출력 데이터의 차이를 계산하여 입력 데이터를 보상하는 방법을 제안한다.

생체응용을 위한 휴대용 단일빔 음향집게시스템 개발 (Development of portable single-beam acoustic tweezers for biomedical applications)

  • 이준수;박연성;김미지;윤창한
    • 한국음향학회지
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    • 제39권5호
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    • pp.435-440
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    • 2020
  • 음향집게는 마이크론 단위의 미세입자를 비접촉 방식으로 조작할 수 있어 다양한 생체공학 응용에 사용되고 있다. 현재까지 음향집게는 in vitro 실험을 목적으로 개발되어 임의파형 발생기와 전력 증폭기와 같은 부피가 큰 고가의 장비를 사용하여 구현하였다. 따라서 이러한 시스템은 이동이 불편하여 한정된 공간에서만 사용이 가능하기 때문에 향후 in vivo 및 임상 실험에 적합하지 않은 구조를 가진다. 따라서 본 논문에서는 이동이 가능한 휴대용 음향집게를 개발하고 그 성능을 평가하였다. 개발한 휴대용 음향집게 시스템은 하나의 Field Programmable Gate Array(FPGA)와 2 개의 펄서로 구현되었으며, Universal Serial Bus(USB) 통신을 이용하여 Personal Computer(PC)에서 송신 주파수 및 펄스 길이 등을 실시간으로 조절이 가능하도록 설계하였다. 개발한 시스템은 최대 20 MHz의 중심 주파수 까지 송신이 가능하며, 미세입자 및 세포를 포획할 수 있는 충분한 힘을 생성할 수 있었다. 개발한 시스템의 성능을 평가하기 위하여 40 ㎛와 90 ㎛ 크기의 폴리스티렌 입자를 포획 및 조정하였다.

광압을 이용한 입자빔 집속 (Particle Beam Focusing Using Radiation Pressure)

  • 김상복;박형호;김상수
    • 대한기계학회논문집B
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    • 제29권1호
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    • pp.110-115
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    • 2005
  • A novel technique for fine particle beam focusing under the atmospheric pressure is introduced using a radiation pressure assisted aerodynamic lens. To introduce the radiation pressure in the aerodynamic focusing system, a 25m plano-convex lens having 2.5mm hole at its center is used as an orifice. The particle beam width is measured for various laser power, particle size, and flow velocity. In addition, the effect of the laser characteristics on the beam focusing is evaluated comparing an optical tweezers type and pure gradient force type. For the pure aerodynamic focusing system, the particle beam width was decreased as increasing particle size and Reynolds number. Using the optical tweezers type, the particle beam width becomes smaller than that of the pure aerodynamic focusing system about $16\%,\;11.4\%\;and\;9.6\%$ for PSL particle size of $2.5{\mu}m,\;1.0{\mu}m,\;and\;0.5{\mu}m$, respectively. Particle beam width was minimized around the laser power of 0.2W. However, as increasing the laser power higher than 0.4W, the particle beam width was increased a little and it approached almost a constant value which is still smaller than that of the pure aerodynamic focusing system. For pure gradient force type, the reduction of the particle beam width was smaller than optical tweezers type but proportional to laser power. The radiation pressure effect on the particle beam width is intensified as Reynolds number decreases or particle size increases relatively.