• Proceedings of the Korean Vacuum Society Conference
• /
• 2014.02a
• /
• pp.402.1-402.1
• /
• 2014

Photonic force microscopy (PFM) is an optical tweezers-based scanning probe microscopy, which measures the forces in the range of fN to pN. The low stiffness leads proper to measure single molecular interaction. We introduce a novel photonic force microscopy to stably map various chemical properties as well as topographic information, utilizing weak molecular bond between probe and object's surface. First, we installed stable optical tweezers instrument, where an IR laser with 1064 nm wavelength was used as trapping source to reduce damage to biological sample. To manipulate trapped material, electric driven two-axis mirrors were used for x, y directional probe scanning and a piezo stage for z directional probe scanning. For resolution test, probe scans with vertical direction repeatedly at the same lateral position, where the vertical resolution is ~25 nm. To obtain the topography of surface which is etched glass, trapped bead scans 3-dimensionally and measures the contact position in each cycle. To acquire the chemical mapping, we design the DNA oligonucleotide pairs combining as a zipping structure, where one is attached at the surface of bead and other is arranged on surface. We measured the rupture force of molecular bonding to investigate chemical properties on the surface with various loading rate. We expect this system can realize a high-resolution multi-functional imaging technique able to acquire topographic map of objects and to distinguish difference of chemical properties between these objects simultaneously.

• Proceedings of the Optical Society of Korea Conference
• /
• 2008.07a
• /
• pp.305-306
• /
• 2008

• Molecular & Cellular Toxicology
• /
• v.3 no.1
• /
• pp.19-22
• /
• 2007

Optic tweezer is powerful tool to investigate biologic cells. Of eukaryotic cells, it was poorly documented regarding to optic trapping to manipulate yeasts. In preliminary experiment to explore yeast biology, interferometric optical tweezers was exploited to trap and manipulate budding yeasts. Successfully, several budding yeasts are trapped simultaneously. We found that the budding direction of the daughter cell was almost outward and the daughter cell surrounded by other yeasts grows slowly or fail to grow. Thus it was assumed that neighboring cells around budding yeast may be critical in budding and the growth of daughter cells. This is first report pertaining to the pattern of yeast budding under the optical trap when multiple yeasts were trapped.

• BMB Reports
• /
• v.52 no.10
• /
• pp.589-594
• /
• 2019

Single-molecule techniques have been used successfully to visualize real-time enzymatic activities, revealing transient complex properties and heterogeneity of various biological events. Especially, conventional force spectroscopy including optical tweezers and magnetic tweezers has been widely used to monitor change in DNA length by enzymes with high spatiotemporal resolutions of ~nanometers and ~milliseconds. However, DNA metabolism results from coordination of a number of components during the processes, requiring efficient monitoring of a complex of proteins catalyzing DNA substrates. In this min-review, we will introduce a simple and multiplexed single-molecule assay to detect DNA substrates catalyzed by enzymes with high-throughput data collection. We conclude with a perspective of possible directions that enhance capability of the assay to reveal complex biological events with higher resolution.

• Proceedings of the Optical Society of Korea Conference
• /
• 2009.10a
• /
• pp.56-57
• /
• 2009

• Journal of the KSME
• /
• v.44 no.10
• /
• pp.62-69
• /
• 2004

이 글에서는 마이크로/나노 스케일의 생체분자를 제어하거나 생체 분자의 기계적 물성치 측정 그리고 더 나아가 생체분자 상호간 작용력을 측정할 수 있는 광핀셋(optical tweezers) 원리 및 응용 예를 소개한다.

• Proceedings of the Optical Society of Korea Conference
• /
• 2008.02a
• /
• pp.237-238
• /
• 2008

• Korean Journal of Optics and Photonics
• /
• v.19 no.2
• /
• pp.132-139
• /
• 2008

In scanning laser optical tweezers, high speed scanning stages are used to manipulate a laser beam spot. Due to the inertia of the stage, the output scanning signal decreases with increased frequency of the input signal. This discrepancy in the signals is difficult to observe since most of the energy from the laser beam is blocked out to avoid CCD damage. In this paper, we propose two methods to alleviate these problems. Firstly, frequency responses of piezo stages are measured to analyze the signal drops and the input signal is compensated accordingly. Secondly, an overlay of the scanning path is drawn on the live monitoring screen to enhance the visibility of the scanning path. The result is a drop-compensated scanning with clear path view.

• The Journal of the Acoustical Society of Korea
• /
• v.39 no.5
• /
• pp.435-440
• /
• 2020

Single-beam acoustic tweezers that are capable of manipulating micron-size particles in a non-contact manner have been used in many biological and biomedical applications. Current single-beam acoustic tweezer systems developed for in vitro experiments consist of a function generator and a power amplifier, thus the system is bulky and expensive. This configuration would not be suitable for in vivo and clinical applications. Thus, in this paper, we present a portable single-beam acoustic tweezer system and its performances of trapping and manipulating micron-size objects. The developed system consists of an Field Programmable Gate Array (FPGA) chip and two pulsers, and parameters such as center frequency and pulse duration were controlled by a Personal Computer (PC) via a USB (Universal Serial Bus) interface in real-time. It was shown that the system was capable of generating the transmitting pulse up to 20 MHz, and producing sufficient intensity to trap microparticles and cells. The performance of the system was evaluated by trapping and manipulating 40 ㎛ and 90 ㎛ in diameter polystyrene particles.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.29 no.1 s.232
• /
• pp.110-115
• /
• 2005

A novel technique for fine particle beam focusing under the atmospheric pressure is introduced using a radiation pressure assisted aerodynamic lens. To introduce the radiation pressure in the aerodynamic focusing system, a 25m plano-convex lens having 2.5mm hole at its center is used as an orifice. The particle beam width is measured for various laser power, particle size, and flow velocity. In addition, the effect of the laser characteristics on the beam focusing is evaluated comparing an optical tweezers type and pure gradient force type. For the pure aerodynamic focusing system, the particle beam width was decreased as increasing particle size and Reynolds number. Using the optical tweezers type, the particle beam width becomes smaller than that of the pure aerodynamic focusing system about $16\%,\;11.4\%\;and\;9.6\%$ for PSL particle size of $2.5{\mu}m,\;1.0{\mu}m,\;and\;0.5{\mu}m$, respectively. Particle beam width was minimized around the laser power of 0.2W. However, as increasing the laser power higher than 0.4W, the particle beam width was increased a little and it approached almost a constant value which is still smaller than that of the pure aerodynamic focusing system. For pure gradient force type, the reduction of the particle beam width was smaller than optical tweezers type but proportional to laser power. The radiation pressure effect on the particle beam width is intensified as Reynolds number decreases or particle size increases relatively.