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In vitro Translation and Methylation of Iso-1-Cytochrome C from Saccharomyces Cerevisiae

  • Paik, Woon-Ki;Park, Kwang-Sook;Tuck, Martin;Kim, Sang-Duk
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1986.12a
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    • pp.505.1-505
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    • 1986
  • The gene for iso-1-cytochrome c for Saccharomyces cerevisiae was recloned into a pSP65 vector containing an active bacteriophage SP6 promoter. The iso-1-cytochrome c gene was cloned as an 856 bp Xho 1-Hind III fragment. When the resulting plasmid was digested at the Hind 111 site 279 bases downstream from the termination codon of the gene and transcribed in vitro using SP6 RNA polymerase, full length transcripts were produced. The SP6 iso-1-cytochrome c mRNA was translated using a rabbit reticulocyte lysate system and the protein products analyzed on SDS polyacrylamide gels. One major band was detected by autofluorography. This band was found to have a molecular weight of 12,000 Da and coincided with the Coomassie staining band of apocytochrome c from S. cerebisiae. The product was also shown to be identical with that of standard yeast apocytochrome c on an isoelectric focusing gel. The in vitro synthesized iso-a-cytochrome c was methylated by adding partially purified S-adenosyl-L-methionine . protein-lysine N-methyltransferase (Protein methylase III; EC 2.1.1.43) from S. cerevisiae along with S-adenosyl-L-methionine to the in vitro translation mixtures. The methylation was shown to be inhibited by the addition of the methylase inhibitor S-adenosyl-L-homocysteine or the protein synthesis inhibitor pu omycin. The methyl derivatives in the protein were identified as $\varepsilon$-N-mono, di and trimethyllysine by amino acid analysis. The molar ratio of methyl groups incorporated to that of cytochrome c molecules synthesized showed that 23% of the translated cytochrome c molecules were methylated by protein methylase III.

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The Effect of Lifestyle, Dietary Habit, Food Preference and Eating Frequency on Sweet Taste Sensitivity and Preference of the Middle School Students (일부 중학생의 생활습관, 식습관, 음식 선호도 및 섭취빈도가 단맛의 민감도와 최적당도에 미치는 영향)

  • Kim, Ga-Hee;Lee, Hong-Mie
    • Journal of Nutrition and Health
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    • v.40 no.6
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    • pp.531-541
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    • 2007
  • This study was conducted to determine factors affecting detection threshold and the optimally-preferred concentration for sweet taste, since investigation into factors decreasing sensitivity or increasing preference for sweet taste is important to prevent overconsumption of simple sugar. Subjects were 70 first-grade middle school students in the Gyeonggi-Do. The detection threshold concentration of sucrose solution and the optimally-preferred sucrose concentration of omija jelly were determined by sensory evaluation. A self-administered questionnaire was used to obtain the information regarding health-related lifestyle practices, dietary habits, preference for and eating frequency of Westernized foods and frequencies of eating foods other than home-made. The detection threshold concentration of sucrose solution and the optimally-preferred sucrose concentration of omija jelly of the subjects were 0.204% and 14.44%, respectively. Subjects who exercise less (< 30 minutes/week except PE class), skipping meals (${\geq}$ 3/week), type of breakfast rather than traditional Korean meal and frequent eating out and buying foods from school tuck-shop tended to have higher sweet taste threshold. There was significant positive correlation between the sucrose threshold concentration and fried chicken preference or eating frequency of hamburger. Those who exercise less (< 30 minutes/week except PE class) did have significantly higher optimally-preferred sweetness of omija jelly, which tended to be higher in the subjects who had more (${\geq}$ 4 out of 10) behavioral or psychological stress symptoms and who had early getting-up time (before 7 am). There was significant positive correlations between the optimally-preferred sweetness of omija jelly and pizza preference, instant noodle eating frequency or sum of eating frequencies of five fast foods. From the results of this study, it was suggested that middle school students should make efforts to stop skipping meals, exercise more, eat Korean traditional breakfast and reduce the frequencies of eating out and using instant/fast foods in order to prevent overconsumption simple sugar.

The Study on the Development of Uniform Design according to Business Types -Focused on the Case of Korean Tobacco and Ginseng Corporation's Uniform Design- (업무 유형에 따른 유니폼디자인 개발에 관한 연구 -한국담배인삼공사 유니폼디자인 사례를 중심으로-)

  • Ahn Min-Young;Lee Youn-Hee;Park Jae-Ok;Suh Mi-A;Jin Sung-Mo
    • Journal of the Korean Society of Clothing and Textiles
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    • v.30 no.2 s.150
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    • pp.306-315
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    • 2006
  • Today, the uniform is perceived being important, because it transmits the unique company image through visualization of images, improves efficiency of work, and increases the feeling of belonging. Therefore, the purpose of this project was to make the winter uniform for the personnel in the manufacturing and the business departments of Korea Tobacco and Ginseng Corporation. The functional, aesthetic, and symbolic aspects were fully considered throughout the design process for the new uniform. To reflect the worker's opinions, the questionnaire method was used. The questionnaire was made up of the questions about the evaluation criteria and design preference for uniform. The result of the survey was reflected on the uniform design. The uniform consists of a jumper and a pair of pants. The jumper for manufacturing workers has two patched pockets with gussets. It has a convertible collar and a horizontal tuck on front panel. It has an open fastener on the front and a flap to cover the fastener. It has two types of pockets inside for functional reason. The welt one is for a wallet and the small one is for a pen. For the material, melanges with two tones of gray color was used, so it gave the sophisticated look on the uniform. The jumper for business workers also has a convertible collar and inside pockets. It has two slant slash pockets with welt. In addition, it has an attachable fur collar which gives thermal protection and aesthetic effect. The fabric has small check pattern and was made of wool. The pants are a basic trouser style with two pleats on the front.

Wearing Condition & Preference of Shirts for Males in Their Twenties and Thirties (20~30대 남성의 셔츠 착용실태 및 선호도 연구)

  • Seong, Hyeyun;Yi, Kyong-Hwa
    • Journal of Fashion Business
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    • v.20 no.5
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    • pp.60-75
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    • 2016
  • This research was conducted to understand the different circumstances for wearing shirts by adult males aged 20-39, and to provide this basic information to the shirt industry prior to developing new functional men's shirts. A total of 345 respondents participated in the survey. Most of questionnaire, frequency, mean and standard deviation were calculated and the differences between the 20s and 30s were analyzed by t-test or ${\chi}^2$ test. The results of the survey are as follows. Grading the satisfaction degree of their body parts, the respondents were relatively unsatisfied with their height, weight, and waist, abdominal and hip circumferences. Majority preferred department stores, discount stores and outlets for purchasing shirts. Many have never owned tailored shirts - men in 20's had less experience with tailoring than men in 30's. The foremost selection criterion for purchasing shirts was fit -during purchase, men in their 20's considered fit more important than men in their 30's. The most preferred unbuttoning of the top button when wearing shirts. For favored collar shapes with one button unbuttoned, the most valued collar angle and style was V-neck shape when unbuttoned, low collar band, collar with unopened collar, and stiff collars. Most shirt designs and details included slim fit, no dart in the front and one dart on the back. Men in 20's more preferred the no dart in the front and one tuck on the back, as compared to men in 30's. On the other hand, men in their 30's preferred one dart shirts than men in 20's. Regarding shirt bands and cuffs, one button and regular collar and one button cuffs with round angle design, were the most preferred, respectively.

A Study on the Tensile Deformation Characteristics of Knits and Appearance Using 3D Digital Virtual Clothing Systems (니트소재의 인장변형 특성과 3D 디지털 클로딩 시스템에 의한 외관표현에 관한 연구)

  • Choi, Kyoung-Me;Kim, Jong-Jun
    • Journal of Fashion Business
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    • v.16 no.2
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    • pp.151-162
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    • 2012
  • The industry-wide development of digital technologies has also affected the textile and fashion industries immensely. The applications of 3D technology, virtual reality, and/or augmented reality systems have helped to create novel fashion brands based on the marriage of IT and textile/fashion industries. 3D digital virtual clothing systems have been developed to help the textile and fashion industries in terms of the planning, manufacturing, marketing and sales sectors. So far, most of the development effort for the 3d virtual clothing systems has been focused on the woven fabrics. The characteristics of woven fabrics differ from those of knitted fabric. Since the physical structures and mechanical properties of the knitted fabrics are definitely different from those of woven fabrics, the simulation process for the knitted fabrics should follow different approaches. The loops in a knitted fabric deform easily. The deformation results in a readily stretchable fabric appearance. Cloth simulation mostly employs models that approximate the mechanical properties of linear elastic planes. This simulation scheme does not, however, describe well enough the behavior of knitted fabrics, which deviate largely from the linear isotropic material characteristics. This study aims at characterizing the tensile deformation and surface textures of a knitted fabric product. Tensile deformation curves for the wale, course, and bias direction are analyzed. The surface texture of the knitted fabric is analyzed by using a 3-dimensional scanning device.

Architecture and Path-Finding Behavior of An Intelligent Agent Deploying within 3D Virtual Environment (3차원 가상환경에서 동작하는 지능형 에이전트의 구조와 경로 찾기 행위)

  • Kim, In-Cheol;Lee, Jae-Ho
    • The KIPS Transactions:PartB
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    • v.10B no.1
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    • pp.1-12
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    • 2003
  • In this paper, we Introduce the Unreal Tournament (UT) game and the Gamebots system. The former it a well-known 3D first-person action game and the latter is an intelligent agent research testbed based on UT And then we explain the design and implementation of KGBot, which is an intelligent non-player character deploying effectively within the 3D virtual environment provided by UT and the Gamebots system. KGBot is a bot client within the Gamebots System. KGBot accomplishes its own task to find out and dominate several domination points pro-located on the complex surface map of 3D virtual environment KGBot adopts UM-PRS as its control engine, which is a general BDI agent architecture. KGBot contains a hierarchical knowledge base representing its complex behaviors in multiple layers. In this paper, we explain details of KGBot's Intelligent behaviors, tuck af locating the hidden domination points by exploring the unknown world effectively. constructing a path map by collecting the waypoints and paths distributed over the world, and finding an optimal path to certain destination based on this path graph. Finally we analyze the performance of KGBot exploring strategy and control engine through some experiments on different 3D maps.

Slacks Pattern Development for Chinese Males in Their 20s (중국 20대 남성의 슬랙스 패턴 개발)

  • Lee, So-Young;Shim, Boo-Ja
    • Journal of Fashion Business
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    • v.12 no.1
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    • pp.93-108
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    • 2008
  • This study held physical measurement for male subjects in their 20s living in Ningbo City, Zhejiang Province in China, showing the biggest market in men's clothes. Their representative lower body types with average shapes and sizes were chosen so as to develop men's slacks patterns with better dimension fitness. The following are the results of the analysis: 1. Slacks patterns were made based on the mean measurement values of 5 people, who were closest to the representative body types, from the index*absolute group of 47 people. The dimensions of application for slacks pattern-making were 77cm(omphalion waist circumference), 91cm(hip circumference), and 103cm(slacks length). 2. For the comparison of high-fitness and more-room pattern dimensions, 5 styles of slacks were made: Korean, Chinese, British, Japanese, and industrial. In the dressing fitness tests, industrial patterns had excellent appearance and Japanese ones had the best function. 3. Based on the analysis results of slacks pattern comparison, the target slacks were experimentally made and received superior evaluation points to the compared slacks patterns in two times of evaluation for appearance and function. 4. The final slacks pattern in this research had the following dimensions: front waist circumference: W(1-2cm with room included)/4-1cm, back waist circumference: W/4+1cm, front hip circumference: H(9$\sim$10cm with room included)/4-1cm, back hip circumference: H/4+1cm, crotch length: H/4-1.5cm, hip length: crotch length-H/12, front crotch extension: H/16-1.5cm, back crotch extension: H/8+3cm, knee line: 33cm below crotch line, front tuck amount: 4cm, back dart amount: 2.5cm(center) and 1.5cm(side), and back dart length: 10cm (center) and 6cm(side).

Numerical Analysis on the Wave Resistance by the Theory of Slender Ships (세장선 이론에 의한 조파저항의 수치 해석)

  • Kim, In Chull
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.23 no.3
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    • pp.1-1
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    • 1987
  • The accurate prediction of the ship wave resistance is very important to design ships which operate satisfactorily in a wave environment. Thus, work should continue on development and validation of methods to compute ship wave patterns and wave resistance. Research efforts to improve the prediction of ship waves and wavemaking resistance are categorized in two major areas. First is the development of higher-order theories to take account of the nonlinear effect of the free surface condition and improved analytical treatment of the body boundary condition. Second is the development of direct numerical methods aimed at solving body and free-surface boundary conditions as accurately as possible. A new formulation of the slender body theory for a ship with constant speed is developed by Maruo. It is quite different from the existing slender ship theory by Vossers, Maruo and Tuck. It may be regarded as a substitute for the Neumann-Kelvin approximation. In present work, the method of asymptotic expansion of the Kelvin source is applied to obtain a new wave resistance formulation in fluid of finite depth. It takes a simple form than existing theory.

Comparison of brand-name school uniform patterns for middle school boys and the development of school uniform patterns by students' body shape, using 3D virtual clothing simulation (3D virtual clothing simulation을 활용한 남자 중학생의 브랜드교복 패턴비교 및 체형별 교복 패턴개발)

  • Shin, Jang-Hee
    • Journal of the Korea Fashion and Costume Design Association
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    • v.23 no.1
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    • pp.143-154
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    • 2021
  • This study attempted to develop winter jackets and pants patterns reflecting the latest comfortable and active school uniform trends, using middle school boy avatars having various body shapes. Jacket and pants pattern-drawing methods and margins differed. Based on the results, research prototypes were prepared. Then, virtual wear prospective drawings, clothing pressure, and appearance were assessed according to body shape. Jackets were assessed using 25 factors, while pants were assessed using 19. Then, correlations between the jackets and pants were analyzed. According to the analysis, the reason why the dart & tuck position and margin were rated low was confirmed. In a virtual wear assessment on jacket patterns by body shape, a significant difference was found in all categories, except for fit and shape at the front bottom, sleeve length on the side, and the center back line. The virtual wear assessment on the pants patterns by body shape also revealed a significant difference in all categories, except for pants length. In jackets, a significant influence was observed around the shoulders and waist in Type 1 and around the belly in Type 2. On the contrary, for pants, a significant influence was found around the hip and waist in Type 1 and around back crotch in Type 2. Therefore, they should be considered when making of jackets and pants. The above results suggested that jacket and pants patterns should vary depending on body shape. It is anticipated that there should be further studies comparing brand-name school uniforms for middle school girls and school uniforms by body shape.

Comparison of Hyang-Sa and Bulchunwee Rituals and Food in Kyungbuk - Focused on Daegu and Andong Areas - (경북 지역의 향사와 불천위제례의 진설과 제수 비교 - 대구와 안동지역 중심으로 -)

  • Choi, Jeong-Hee;Park, Geum-Soon
    • Korean journal of food and cookery science
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    • v.24 no.6
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    • pp.801-810
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    • 2008
  • The principal objective of this study was to assess Korea's traditional ritual food culture, and to compare two types of ancestral rites the Hyang-Sa and Bool-cheon-wi rites which were held in the Daegu and Andong regions of Korea. In this study, we describe the performance of the Bool-cheon-wi rites held by two head families located near the Andong area namely, the head family of Seoae Ryu Seong-Ryong(1542-1607)(Seoae) who was well-respected for his writings and personality, and the head family of Dangye Ha Wee-Ji(1412-1456)(Dangye) who was well-known and famous as one of the members of the Sa-ryuk-sin. This research was conducted via diredt engagement in these memorial services and several interviews with the families. The results were summarized as follows; Foods used in the Hyang-sa rites including Mae(bap), Kook, Jaban(Jogee), Po(dried fish), Juck, boiled and seasoned vegetables, fruits, confections, and liquor. Jobap and Ssalbap were used as Mae at SD(Seo Dalsung), and PMPH(Pahoi Myogul Habin Park) used Jo, Hyunmy, Pi and Susu in the raw. The dishes on the table were arranged as follows. A wine cup was placed in the first row, Po(a dried pollack), and jujube and nuts in the second row, Ryukpo(slices of dried beef), Sangeogogi were set at the third row, and Soegogi, pork, Mu, and Minary were placed in the fourth row, and the head of the pig was placed in the center of the table at SD. A wine cup, Soegogi, and pork were positioned in the first row, Mu, Minary, Pi and Susu were placed in the second row, and Jogee, Jo, and Hyunmy were placed in the third row at PMPH. The sacrificial foods offered for Bool-cheon-wi rites were as follows; Mae(bap) Kook noodle Jogee Tang(stew) Po Juck Tucks boiled, seasoned and salted vegetables Jeon fruit confectioneries liquor(chungju). The head family of Seoae Yu Seong-Ryong utilized 5 types of Tang(meat stew, fish stew, chicken stew, vegetable stew, seafood stew), whereas the head family of Dangye Ha Wee-Ji utilized 3 kinds of Tang(meat stew, fish stew, vegetable stew). As a basic Tuck, Shi-roo-tuck(a steamed rice cake), piled up to 25 layers, was primarily utilized. In particular, Jung-Gae(Seoae's favorite food) was placed on the table. For grilled-meat food(Juck), Yu's family used raw meat and Ha's family the half-cooked meat. The main types of Jucks used were meat-Juck, fish-Juck, chicken-Juck, and these were not served one by one. Hyang-sa and Bool-cheon-wi rites have an the educational function, in that they allow for the carrying out of filial duties by a heartfelt performance of performing the ceremony, by taking great care from the pre-rite preparations to the post-rite period. In addition, these rites have various meanings, as events that strengthen the ties of blood relations of ancestors and themselves, and to promote and harmonize family friendships, they may also have religious meaning in the culture, as prayers are offered that all the family's descendants may be blessed, live long and enjoy abundance whlie respecting their ancestors. As for the role of Hyang-sa and Bool-cheon-wi rites in today's nuclear family society, it can be said that these rites remain especially important as a method to strengthen community consciousness by fostering an understanding of the meaning of existence itself, and thus inspiring the roots of consciousness.