• Title/Summary/Keyword: tubing

Search Result 248, Processing Time 0.022 seconds

Pattern of Molecular Aggregation of Ginsenosides in Aqueous Solution (수용액(水溶液)에서 인삼배당체(人蔘配糖體)의 분자결합양상(分子結合樣相))

  • Park, Hoon;Lee, Mee-Kyoung;Park, Qwi-Hee
    • Applied Biological Chemistry
    • /
    • v.29 no.2
    • /
    • pp.198-206
    • /
    • 1986
  • For the information on micellization at each ginsenoside level aqueous solution of purified saponin of Panax ginseng root was dialyzed through dialysis tubing (MW 12,000) or eluted through Bio-Gel P-2 (MW 200-2,000) and analysed for ginsenosides by high performance liquid chromatography. Ginsenosides can be classified into three groups depending upon molecular aggregation pattern and spatial arrangement of hydrophilic parts in molecule. Group I that is large micelle former(aggregation number: above 10) and one side hydrophilic part (HP) includes $ginsenoside\;Rb_1$, $Rb_2$, Rc and Rd (diols). Group II thai is small micelle former (aggregation number:>10-1) and semi-two sales HP includes $Rg_2$, Rf (triol) and $Rg_3$ (diol). Group III that is no micelle former (aggregation number: 1) and two sides HP includes Re and $Rg_1$ (triol).

  • PDF

In vivo Antioxidant Effects of Aralia elata Seemann Ethanol Extract Administered with Benzo($\alpha$) pyrene

  • Nam, Sang-Myung;Chung, Cha-Kwon
    • Preventive Nutrition and Food Science
    • /
    • v.4 no.1
    • /
    • pp.52-56
    • /
    • 1999
  • This study has examined the effects of Aralia elata Seemann ethanol extract on antioxidant enzyme systems inrats along with benzo($\alpha$) pyrene(B(a)P) administration . The ethanol extract of Aralia elata Seemann (50mg/kg body wt.) was fed to rats for 4 weeks by stomach tubing. The extract administration increased antioxidant activities of glutathione sulfur transferase(GST) comparing to the control. also total superoxide dismutase(SOD) and Cu, Zn-SOD activities were stimulated. Catalase activities were increased by 50% with the extract feeding compared to the control . Combined administration of B($\alpha$)P and the extract increased GST activity in B($\alpha$) P group. Although total SOD acitivity was decreased , Cu, Zn-SOD was greately increased from 0.10unit to 0.18 unit and catalase activity also was increased compared to the group of B($\alpha$) P. GST activity in CLE group was 1.32 unit, increased by 33% comparing to the group CL of 0.99unit. Cu, Zn-SOD and catalase activities in thegroup fed high fat and ethanol extracts were increased by 25% and 39%, respectivley comparing to the group of high fat. In addition , total SOD was decreased but, Cu, Zn-SOD acitivity was increased from 0.09 unit to 0.18unit. Catalase activity was 76.05 unit in the group of B($\alpha$) P and extract comparing to 65.26 units in B($\alpha$)P group. Serum $\alpha$-tocopherol of rat was markedly increased by theextract. Administration of B9$\alpha$)P reduced $\alpha$-tocopherol levels in the serum, on the other hand, lard in the diet increased $\alpha$-tocopherol levels in the serum. The above results indicate that Aralia bud exerts antioxidant functions in vivo against B($\alpha$)P. Further research may be necessary for the identification fo the biologically active material.

  • PDF

The Apparent Digestibility of Corn By-products for Growing-finishing Pigs In vivo and In vitro

  • Guo, Liang;Piao, Xiangshu;Li, Defa;Li, Songyu
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.17 no.3
    • /
    • pp.379-385
    • /
    • 2004
  • Two trials in vivo and in vitro were conducted, in vivo to determine the apparent digestibility of gross energy, crude protein, dry matter, acid detergent fiber, neutral detergent fiber and apparent digestible energy in 10 corn by-products. In vivo the diets included one basal corn diet, four corn gluten meal diets, four corn distillers dried grains with solubles diets and two corn distillers dried grains diets using the different methods, 12 crossbred barrows weigh $40{\pm}$1.6 kg were allocated into individual metabolic crate, according to a $6{\times}6$ Latin square design. In vitro using flask technique, filter bag technique and dialysis tubing technique, the digestibilities of gross energy, crude protein and dry matter in corn gluten meal and corn distillers dried grains with solubles were investigated. Pepsin, pancreatin, intestinal fluid, rumen fluid and cellulase were used in incubation. The results showed that correlation coefficient was 0.73 in corn distillers dried grains with solubles between the digestibility of crude protein and acid detergent fiber in vivo (p<0.01); and correlation coefficient was 0.68 in corn distillers dried grains with solubles between the digestibility of gross energy and neutral detergent fiber in vivo (p<0.01). Apparent digestible energy (DE) of corn by-products in pig total tract was predicted by the percentage of crude protein (CP) and the content of gross energy (GE) in feedstuff. The equation: DE=5,601.09+26.69$\times$CP %-0.5904$\times$GE, ($R^2=0.72$). In vitro, filter bag technique was more convenient; furthermore, the digestibility for the treatments (pepsin+pancreatin+rumen fluid and pepsin+pancreatin+cellulase) was better.

The Clinical Analysis of Two Layer Vasovasostomy Using Silicone Tube (실리콘 관을 이용한 이층 정관정관 문합술)

  • Choi, Eui Chul;Choi, Hwan Jun;Yun, Jong Hyun;Kim, Yong Bae
    • Archives of Plastic Surgery
    • /
    • v.36 no.2
    • /
    • pp.183-187
    • /
    • 2009
  • Purpose: Because of the volume of elective sterilizations performed in the world during the past decade, the vasectomy was a popular method for male sterilization in Korea and this, in turn, had been followed by an increase in the number of patient requiring vasectomy reversal with the high rate of subsequent divorce and remarriage. Recently, many authors have reported high success rates of vasovasostomy using microsurgical techniques and we performed modified two layer vasovasostomy with intravasal silicone tube to increase postoperative patency and pregnacy rate. Methods: Microscopic vasovasostomy was performed in 9 patients and their average age was $35.78{\pm}1.36years$(from 28 years to 44 years) from June 2006 to June 2008 at our department using modified two layer vasovasostomy with silicone tube insertion. Standard Guibor silicone tube, consisting of two 17.7 cm, 0.064 cm diameter, malleable, stainless steel probes connected by 29 cm of silicone tubing wedged onto disposable probes, were used in all cases. Results: Success rates were 88.8 % for patency and pregnancy 44.4 % for pregnancy in modified two layer vasovasostomy with silicone tube insertion. The patency rates were higher in cases of long postoperative day and in cases of short duration of vasectomy and vasovasostomy. Conclusion: We used a modified method to correspond the patency and pregnancy rate in microscopical modified two layer vasovasostomy using the intravasal silicone tube permanently. This method brings normal patency in microsurgical vasovasostomy because the silicone tube prevent obstruction of anastomosed site of the vas permanently.

Correlation between in vitro release and in vivo bioavailability of Propranolol.HCI from Poly(vinyl alcohol) Hydrogel Suppositories (폴리비닐알코올 하이드로겔 좌제로부터 프로프라놀롤의 in vitro 방출과 in vivo 생체이용률간의 상관성)

  • Kim, Ho-Jeong;Ku, Young-Soon
    • Journal of Pharmaceutical Investigation
    • /
    • v.28 no.4
    • /
    • pp.275-282
    • /
    • 1998
  • In order to develop a desirable in vitro release which correlates well with in vivo bioavailability, hollow type suppository containing Propranolol HCl(PPH) powder in the cavity and conventional type suppository with dispersed PPH in the base were prepared. Polyvinyl alcohol (PVA) hydrogel as a base and PPH as a model drug were used for the preparation of suppository. The rates of drug release from the suppositories were studied by Paddle method, Muranish method, Dialysis tubing method and Rotating dialysis cell method. The release profiles from suppositories using the four different release tests were compared. After a rectal administration in rat, the mean $C_{max}$ of hollow type suppository was significantly lower than that of conventional type, but $T_{max}$, $AUC_{0{\to}12}$ and MRT of hollow type were significantly higher 1.6 times, 1.2 times and 1.9 times than those of conventional type, respectively. The computer program was used to simulate plasma concentration from in vitro released amounts of drug and in vivo pharmacokinetic parameters. Based on comparison of the simulated bioavailability from computer program with experimental bioavailability in rat we have found out in vitro release test which correlates well with in vivo bioavailability. Our results have shown the best correlation between in vitro release and in vivo bioavailability in PPH-PVA hydrogel hollow type suppository for the paddle method and conventional type suppository for the rotating dialysis cell method. In this work we propose that PPH-PVA hydrogel suppository shows in vitro-in vivo correlation. This data should help to optimize the formulation of the drug and provide a basis for quality control procedures.

  • PDF

SELF-ADHESION OF LOW-VISCOSITY COMPOSITES TO DENTIN SURFACE (상아질에 대한 저점도 복합레진의 자가접착에 관한 연구)

  • Cho, Tae-Hee;Choi, Kyoung-Kyu;Park, Sang-Hyuk;Park, Sang-Jin
    • Restorative Dentistry and Endodontics
    • /
    • v.28 no.3
    • /
    • pp.209-221
    • /
    • 2003
  • The objectiveness of this study was to evaluate whether low-viscosity composite can bond effectively to dentin surface without bonding resin. The low-viscosity composites being 50wt% filler content were made by the inclusion of bonding resin of two self-etching systems(Cleafil SE Bond, Unifil Bond) varied with contents as 0, 10, 20, 30, 40, 50wt%. Exposed dentin surfaces of extracted 3rd molars are used. Dentin bond strengths were measured. The tests were carried out with a micro-shear device placed testing machine at a CHS of 1mm/min after a low-viscosity composite was filled into an iris cut from micro tygon tubing with internal diameter approximately 0.8mm and height of 1.0mm. 1 Flexural strength and modulus was increased with the addition of bonding resin. 2. Micro-shear bond strength to dentin was improved according to content of bonding resin irrespective of applying or not bonding resin in bonding procedure, and that of Clearfil SE Bond groups was higher than Unifil Bond. 3. There were no significant difference whether use of each bonding resin in bonding procedure for S-40, S-50, U-50(p>0.05). 4. In SEM examination, resin was well infiltrated into dentin after primed with self-etching primer only for S-50 and U-50 in spite of the formation of thinner hybrid layer. Low viscosity composite including some functional monomer may be used as dentin bonding resin without an intermediary bonding agent. It makes a simplified bonding procedure and foresees the possibility of self-adhesive restorative material.

Effect of Injector Cooling on Ignition of Cryogenic Spray (분사기 냉각이 초저온 분무의 점화에 미치는 영향)

  • Kim, Do-Hun;Lee, Jin-Hyuk;Koo, Ja-Ye
    • Journal of the Korean Society for Aeronautical & Space Sciences
    • /
    • v.40 no.3
    • /
    • pp.222-229
    • /
    • 2012
  • The cooling of a injector effects on the vapor pressure of cryogenic oxidizer spray, and it decides the phase transition point at the ignition process, when the combustion chamber pressure increases drastically. The phase transition of oxidizer spray affects the ignition characteristics, and several ignition tests with the LOx/$GCH_4$ uni-element coaxial swirl injector was performed in the different initial temperatures of oxidizer injector, in order to investigate the effect of injector cooling on the ignition transient characteristics. At the transition point of oxidizer phase, where the combustion chamber pressure increased over the LOx vapor pressure, the temporary quenching phenomenon of the flame occurred. The lower temperature of chilled down injector and tubing tends to move up the phase transition earlier.

Relationship Between the Initiation and Propagation of SCC and the Electrochemical Noise of Alloy 600 for the Steam Generator Tubing of Nuclear Power Plants

  • Kim, Y.S.;Nam, H.S.;Kwon, Y.H.;Kim, S.W.;Kim, H.P.;Chang, H.Y.
    • Corrosion Science and Technology
    • /
    • v.9 no.2
    • /
    • pp.57-66
    • /
    • 2010
  • Since nuclear power plants are being operated under high temperature and high pressure, on-line monitoring technique to detect corrosion could be more effective than off-line method in shut-off period. In this operating condition, electrochemical noise method may be suitable to monitor the corrosion. This paper aims the analysis on the relation between the cracking and electrochemical noise signal of Alloy 600 under U-bending. When electrochemical noise monitoring technique was used during SCC test, it was judge to be obvious that if cracks generate, its generation can be detected by electrochemical current noise. Cracking-related noise was defined as the noise showing 5~10 times greater than the average value of background noise bands. On the base of crack noise, crack initiation time was determined. From SCC test and electrochemical noise monitoring in $25^{\circ}C$, 0.1 M $Na_2S_4O_6$ solution (Reverse U-Bended Alloy 600 SE+), average crack initiation time was obtained as 9,046 seconds and from its initiation time, it could be defined that net crack propagation rate is the crack length divided by ${\Delta}T$(= total test period - crack initiation time). Therefore, average net crack propagation rate was obtained to be $1.18{\times}10^{-9}\;m/s$.

Material Integrity Assessment for a Ni Electrodeposit inside a Tube

  • Kim, Dong-Jin;Kim, Myong Jin;Kim, Joung Soo;Kim, Hong Pyo
    • Corrosion Science and Technology
    • /
    • v.6 no.5
    • /
    • pp.233-238
    • /
    • 2007
  • Due to the occasional occurrence of a localizedcorrosion such as a SCC and pitting in steam generator tubing(Alloy 600), leading to a significant economical loss, an effective repair technology is needed. For a successful electrodeposition inside a tube, many processes should be developed. Among these processes, an anode to be installed inside a tube, a degreasing condition to remove any dirt and grease, an activation condition for a surface oxide elimination, a strike layer forming condition which needs to be adhered tightly between an electroforming layer and a parent tube and a condition for an electroforming layer should be established. Through a combination of these various process condition parameters, the desired material properties can be acquired. Among these process parameters, various material properties including a mechanical property and its variation along with the height of the electrodeposit inside a tube as well as its thermal stability and SCC resistance should be assessed for an application in a plant. This work deals with the material properties of the Ni electrodeposits formed inside a tube by using the anode developed in this study such as the current efficiency, hardness, tensile property, thermal stability and SCC behavior of the electrodeposit in a 40wt% NaOH solution at $315^{\circ}C$. It was found that a variation of the material properties within the entire length of the electrodeposit was quite acceptable and the Ni electrodeposit showed an excellent SCC resistance.

Angiotensin I-Converting Enzyme Inhibitory Activity of the ${\kappa}-Casein$ Fragments Hydrolysated by Chymosin, Pepsin, and Trypsin (${\kappa}-Casein$의 Chymosin, Pepsin 및 Trypsin 가수분해물에 대한 안지오텐신 변환효소 저해효과의 탐색)

  • Oh, Se-Jong;Kim, Sae-Hun;Kim, Sang-Kyo;Baek, Young-Jin;Cho, Kyung-Hyun
    • Korean Journal of Food Science and Technology
    • /
    • v.29 no.6
    • /
    • pp.1316-1318
    • /
    • 1997
  • The isolated ${\kappa}-Casein$ on gel permeation chromatography was hydrolyzed by chymosin, trypsin, and pepsin. The 3% TCA soluble portion of the hydrolysates were dialyzed on the angiotensin-I converting enzyme (ACE) inhibition rate (%,) and inhibitory activity $(IC_{50})$ were determined. The trypsin hydrolysate exhibited the highest ACE inhibition rate while the chymosin hydrolysation showed the lowest activity. The hydrolysate was dialyzed using dialysis membrane with various molecular cut-offs, and $IC_{50}$ was determined. As the pore size of the dialysis tubing increased, the ACE inhibitory activity decreased.

  • PDF