• Food Science and Preservation
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• v.16 no.5
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• pp.675-679
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• 2009

We investigated how Germanium (Ge) application, and the application method, affected 'Niitaka' (Pyrus pyrifolia) fruit quality in a conventional orchard. Ge-treated pears weredark yellow in color, indicating appropriate maturation. Other fruit characteristics, and weight, did not significantly differ between tests and controls. Compared to controls, fruit from Ge-treated trees had more soluble solids, a lower acid content, and fewer stone cells, which reduced fruit chewiness. Ge-treated trees showed a higher Ge concentration in fruit but a lower calcium (Ca) concentration. Other fruit mineral nutrients, includingP, K, and Mg, did not differ in level between Ge-treated fruit and controls. Ge-treated fruit showed greater firmness and a heavier specific weight during 6 months of storage, compared to control fruit. Therefore, Ge treatment reduced fruit pithiness, which can be a problem in 'Niitaka' pears. Overall, there was no significant difference between Ge-treated and control fruit qualities, but a combined Ge treatment (fertilization plus foliar application plus trunk injection) slightly improved fruit quality.

• The Plant Pathology Journal
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• v.39 no.3
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• pp.290-302
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• 2023

Abamectin offers great protection against Bursaphelenchus xylophilus, a well-known devastating pathogen of pine tree stands. Trunk injection of nematicides is currently the most preferred method of control. This study aimed to evaluate the potency of the commonly used formulations of abamectin against B. xylophilus. Twenty-one formulations of abamectin were evaluated by comparing their sublethal toxicities and reproduction inhibition potentials against B. xylophilus. Nematodes were treated with diluted formulation concentrations in multi-well culture plates. And, populations preexposed to pre-determined concentrations of the formulations were inoculated onto Botrytis cinerea culture, and in pine twig cuttings. Potency was contrastingly different among formulations, with LC₉₅ of 0.00285 and 0.39462 mg/ml for the most, and the least potent formulation, respectively. Paralysis generally occurred at an application dose of 0.06 ㎍/ml or higher, and formulations with high sublethal toxicities caused significant paralysis levels at the tested doses, albeit the variations. Nematode reproduction was evident at lower doses of 0.00053-0.0006 ㎍/ml both on Botrytis cinerea and pine twigs, with significant variations among formulations. Thus, the study highlighted the inconsistencies in the potency of similar product formulations with the same active ingredient concentration against the target organism, and the need to analyze the potential antagonistic effects of the additives used in formulations.

• The Plant Pathology Journal
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• v.39 no.1
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• pp.75-87
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• 2023

The pine wood nematode (PWN), Bursaphelenchus xylophilus is a well-known devastating pathogen of economic importance in the Republic of Korea and other countries. In the Republic of Korea, trunk injection of nematicides is the preferred method of control. In this study, the efficacy of 16 locally produced formulations of emamectin benzoate against the PWN are compared through determining their sublethal toxicities and reproduction inhibition potentials. Nematodes were treated with varying concentrations of the tested chemicals in multi-well culture plates, and rates of paralysis and mortality were determined after 24 h. Reproduction inhibition potential was tested by inoculating pre-treated nematodes onto Botrytis cinerea, and in pine twig cuttings. Despite the uniformity in the concentration of the active ingredient, efficacy was contrastingly different among formulations. The formulations evidently conformed to three distinct groups based on similarities in sublethal activity (group 1: LC₉₅ of 0.00768-0.01443 mg/ ml; group 2: LC₉₅ of 0.03202-0.07236 mg/ml, and group 3: LC₉₅ of as high as 0.30643-0.40811 mg/ml). Nematode paralysis generally occurred at the application dose of 0.0134-0.1075 ㎍/ml, and there were significant differences in nematode paralysis rates among the products. Nematode reproduction was only evident at lower doses both on B. cinerea and pine twigs, albeit the variations among formulations. Group 1 formulations significantly reduced nematode reproduction even at a lower dose of 0.001075 ㎍/ml. The variations in efficacy might be attributed to differences in inert ingredients. Therefore, there is need to analyze the potential antagonistic effects of the large number of additives used in formulations.

• Korean Journal of Bronchoesophagology
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• v.4 no.1
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• pp.96-100
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• 1998

Lymphangiomas are congenital malformations of the lymphatic system. Cervicofacial lymphangioma represents 75% of all lymphangiomas. Surgical excision has been the treatment of choice, however the reported results have been unsatisfactory. Various sclerosants have been tried to treat lymphangiomas, with variable results and considerable side effects. Herein we report the results of treatment using intralesional picibanil for lymphangioma. Between January 1996 and January 1998, 16 patients with lymphangiomas, 10 boys and 6 girls, were treated with intralesional picibanil injections. All cases were treated as a primary therapy. Eight lymphagiomas were located in the neck, and 2 in the cheek, 2 in the parotid, 2 in the trunk, 1 in the oropharynx, 1 in the thigh. Dose and method of intralesional injection was similar to that reported by Ogita in 1987. Complete regression was observed in 10 cases and marked regression(> 75% size decrease) in 2 cases and moderate regression(75%-25% size decrease) in 2 cases and poor regression(< 25% size decrease) in 2 cases. No serious side effect was observed except fever lasting for 2-3 days. Intralesional injection of picibanil for lymphangiomas represents a safe, easy and effective way of treatment with high success rate. Picibanil injection can be used as a primary therapy for lymphangiomas.

• The Plant Pathology Journal
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• v.40 no.2
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• pp.139-150
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• 2024

Huanglongbing (HLB) is a disease caused by the phloem-limited Candidatus Liberibacter asiaticus (CLas) that affects the citrus industry worldwide. To date, only indirect strategies have been implemented to eradicate HLB. Included among these is the population control of the psyllid vector (Diaphorina citri), which usually provides inconsistent results. Even though strategies for direct CLas suppression seem a priori more promising, only a handful of reports have been focused on a confrontation of the pathogen. Recent developments in polymer chemistry have allowed the design of polycationic self-assembled block copolymers with outstanding antibacterial capabilities. Here, we report the use of polymeric nano-sized bactericide particles (PNB) to control CLas directly in the phloem vasculature. The field experiments were performed in Rioverde, San Luis Potosí, and is one of the most important citrusproducing regions in Mexico. An average 52% reduction in the bacterial population was produced when PNB was injected directly into the trunk of 20 infected trees, although, in some cases, reduction levels reached 97%. These results position PNB as a novel and promising nanotechnological tool for citrus crop protection against CLas and other related pathogens.

• Journal of Korean Society of Forest Science
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• v.84 no.1
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• pp.22-30
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• 1995

Responses of Pospam-injected chestnut and pine trees to drill wound were examined. Pospam was injected into the stem by just filling up the drill wound in September 10, 1993. The diameter of the injection wound were 0.5 and 1cm. DBH of chestnut trees and pine trees injected were 10, 15, 20 and 10, 20, 30cm, respectively. The results of tree response were examined in June, 1994. None of them showed any symptom of decay by the time. However, sapwood under the bark was remarkably discolored. The discoloration was more severe in 1cm-injection wound than in 0.5cm one. The severity of discoloration was not differed between species and among DBH applied. The sapwood split was longer in 1cm-injection wounded trees than in 0.5cm trees and longer in chestnut trees than in pine trees. From the split, callus grew out and almost closed the splits. In the trunk injection tested, the damage was mare severe in 1cm injection wound of chestnut trees than in any other combination.

• Korean journal of applied entomology
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• v.17 no.3 s.36
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• pp.155-160
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• 1978

This experiment was carried out to develope a technique for labelling pine gall midge with ·radioactive phosphorus and calcium. This technique evolved would help to obtain basic information on the appropriate concentration of $^{32}P\; and\; ^{45}Ca$ in tagging and determination of adult flight ranges which considered as one of the factors in dissemination. 1. Labelling at the larval stage of pine gall midge by injection of $^{45}Ca$ into pine tree trunk appeared not to be effective for tagging the adults because radioactivity was gradually decreased to background by emergence. 2. The radioactivities of larvae in the galls gradually increased in September but from October 1 the activity was gradually decreased for 10 days then equilibrium was maintained thereafter. The results seem to indicate that larval diet termination occured early October. 3. A maximum level of radiation was detected at 3 weeks after $^{32}P$ injection and thereafter it was gradually reduced. This result implies that $^{32}P$ is not appropriate radioisotope in labelling pine gall midge through trunk injection method. 4. After washing in running water for 10 minutes, the optimum$^{32}P$ concentration for treated overwintered larvae and pupae was found to be 0.5 micro curie per ml. for 30 minutes dipping.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.32 no.4
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• pp.308-316
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• 2006

Objectives Despite considerable advances in technique, experience and skill, the precise place of surgery in the treatment of facial nerve injury remains uncertain. We designed a facial nerve crush injury model in rats and evaluated the recovery of crushed nerve which is the most common injury type of facial nerve using adenovirus vector mediated in vivo gene transfer of Brain derived neurotrophic factor(BDNF). Materials and methods In 48 Sprague Dawley rats, we made a facial nerve crush injury model to main trunk before the furcation, and injected a $10^{11}$pfu adenoviral BDNF in experimental group(BDNF adenoviral injection group; ad-BDNF) and $3{\mu}l$ saline in control group(Saline injection group; saline). After a period of regeneration from 10 to 40 days, nerve regeneration was evaluated with functioinal test (vibrissae and ocular movement), electrophysiologic study(threshold, peak voltage, conduction velocity) and histomorphometric study of axon density. Results Vibrissae and ocular movement, threshold and conduction velocity improved as time elapse in both group, however axon density was increased significantly only in experimental group. Functional test in 10 days and 20 days showed no difference between experimental group and control group. Vibrissae movement, threshold, conduction velocity and axon density in 30 days revealed that the regeneration in quality of experimental group was significantly superior to that of control group. Conclusion In general, there is tendency for nerve regeneration in experimental group (BDNF-adenovirus injection group) during 40 days, functional recovery was detected successfully after facial nerve crush in 30 days postoperatively.

• Korean Journal Plant Pathology
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• v.12 no.3
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• pp.324-330
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• 1996

암수 딴그루인 참다래 품종 중에서 우리나라에서 재배되고 있는 대표적인 결실수(암나무)인 Hayward와 수분수(숫나무)인 Matua에서 궤양병의 발생 차이를 조사한 결과 Hayward의 이병엽율은 18.0%인 반면에 Matua의 이병엽율은 2.4%였다. 또한 Hayward와 Matua의 잎에 형성된 병반에서 분리된 궤양병균의 균총수는 생체중 1 g당 각각 1.8$\times$10\ulcorner개와 2.1$\times$10\ulcorner개로 Hayward에서 Matua보다 약 10배 가량 많은 궤양병균이 검출되었다. Hayward 신초에 존재하는 궤양병균의 밀도를 조사한 결과 줄기 조직보다는 잎 조직에서 많은 궤양병균이 검출되었으며 새로 나온 잎일수록 궤양병균의 밀도는 낮게 검출되었다. 궤양병 방제를 위하여 스트렙토마이신 수간주입에 의한 궤양병 치료효과는 50.7~53.7%였다. 수간주입시 약량을 주당 2 L에서 3 L와 4 L로 증가시킴에 따라 치료효과도 32.3%에서 58.1%와 71.0%로 각각 증대되었는데, 수간주입 약제와 수간주입 방법의 개선을 통하여 궤양병 치료효과를 더욱 증대시킬 수 있을 것으로 기대된다.

• KSBB Journal
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• v.16 no.1
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• pp.71-75
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• 2001

Acanthoside-D, contained in Acanthopanax chilsanensis is known as a ginseng-like substance. This work was focused to set up analytical and preparative conditions for Acanthoside-D purification. The ethanol extract from the powder of the trunk of Acanthopanax chilsanensis was partitioned with hexane. A $\mu$-Bondapak $C_{18}$ (3.9$\times$300 mm, $10\mu\textrm{m}$) column was used to separate Acanthoside-D from the trunk of Acanthopanax chilsanensis. From the experimental results, the mobile phase used for isolating Acanthoside-D from the extract was water/acetonitrile/methanol=80/14/6 %(v/v). The flow rate of mobile phase was 1.0 mL/min, and UV wavelength was fixed at 210 nm. Finally on a semi-preparative column (3.9$\times$ 300 mm, $15\mu\textrm{m}$, Lichrospher 100RP-18) with the same mobile phase composition, the allowable maximum injection volume increased to 250 $\mu\ell$