• Asian-Australasian Journal of Animal Sciences
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• v.30 no.11
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• pp.1633-1642
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• 2017

Objective: Evaluation of exercise effects in racehorses is important in horseracing industry and animal health care. In this study, we compared metabolic patterns between before and after exercise to screen metabolic biomarkers for exercise effects in thoroughbreds. Methods: The concentration of metabolites in muscle, plasma, and urine was measured by $^1H$ nuclear magnetic resonance (NMR) spectroscopy analysis and the relative metabolite levels in the three samples were compared between before and after exercise. Subsequently, multivariate data analysis based on the metabolic profiles was performed using orthogonal partial least square discriminant analysis (OPLS-DA) and variable important plots and t-test was used for basic statistical analysis. Results: From $^1H$ NMR spectroscopy analysis, 35, 25, and 34 metabolites were detected in the muscle, plasma, and urine. Aspartate, betaine, choline, cysteine, ethanol, and threonine were increased over 2-fold in the muscle; propionate and trimethylamine were increased over 2-fold in the plasma; and alanine, glycerol, inosine, lactate, and pyruvate were increased over 2-fold whereas acetoacetate, arginine, citrulline, creatine, glutamine, glutarate, hippurate, lysine, methionine, phenylacetylglycine, taurine, trigonelline, trimethylamine, and trimethylamine N-oxide were decreased below 0.5-fold in the urine. The OPLS-DA showed clear separation of the metabolic patterns before and after exercise in the muscle, plasma, and urine. Statistical analysis showed that after exercise, acetoacetate, arginine, glutamine, hippurate, phenylacetylglycine trimethylamine, trimethylamine N-oxide, and trigonelline were significantly decreased and alanine, glycerol, inosine, lactate, and pyruvate were significantly increased in the urine (p<0.05). Conclusion: In conclusion, we analyzed integrated metabolic patterns in the muscle, plasma, and urine before and after exercise in racehorses. We found changed patterns of metabolites in the muscle, plasma, and urine of racehorses before and after exercise.

• Analytical Science and Technology
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• v.19 no.1
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• pp.96-102
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• 2006

Trimethylamine (TMA) is one of the difficult odorous compounds for the collection and analysis. Although sulfuric acid absorption and/or sulfuric acid impregnated filter method are commonly recommended for its sampling, these methods also suffer from difficulties involved in sample treatment and operational procedures. Hence, as an ancillary approach to measure TMA, we investigated the combination of bag sampling and SPME analysis for TMA measurements. For the purpose of our study, we investigated the following three subjects: 1) temporal variability of standard storage, 2) bag loss effect of TMS, and 3) TMA loss due to repetitive analysis of an identical bag sample. According to our storage test up to 7 or 20 dyas, TMA loss were found to occur up to 40 to 50% within relatively short period of up to 48 hrs depending on its concentration ranges. When the tests were made for bag loss by transferring TMA standards across different size bags, we were able to find that the extent of bag loss are not significant with 5 to 20% loss rate. Finally, the TMA sorptive loss via its exposure to SPME fiber was generally estimated to run from 2 to 3%.

• Journal of Radiation Industry
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• v.6 no.1
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• pp.49-54
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• 2012

Various anion exchange membranes were prepared by radiation graft copolymerization of vinylbenzyl chloride onto fluorinated films and subsequent quaternization with various tertiary amines such as trimethylamine, N,N-dimethylbuthylamine, N,N-dimethylaniline, and N-methylpiperidine. The quaternizations of the anion exchange membranes were confirmed by measuring of the ion exchange capacities of the membranes. The mechanical properties and the water uptakes were also measured. The elongation at break was found to be largely dependent on the fluorinated film, the quateranry ammonium, and the degree of grafting. The results indicate that the poly (ethylene-alt-tetrafluoroethylene) with quaternized trimethylamine moiety exhibits higher flexible property compared to the other prepared anion exchange membranes.

• Proceedings of the Microbiological Society of Korea Conference
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• 2005.05a
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• pp.223.4-223
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• 2005

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2002.10a
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• pp.64-64
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• 2002

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2004.05a
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• pp.179-180
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• 2004

• The Transactions of the Korean Institute of Electrical Engineers C
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• v.48 no.10
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• pp.710-715
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• 1999

The purpose of this work is to develop a high-efficiency air cleaning system for air pollutants such as particulate and gaseous state in indoor environments. In order to enhance a removal efficiency of gaseous state pollutants, we suggested that pulsed discharge plasma be combined with $TiO_2$ photocatalyst (photocatalytic plasma air cleaning unit). We investigated experimentally the basic characteristics of photocatalytic plasma air cleaning unit and measured air pollutants removal efficiency. The wavelength of light radiated from pulsed discharge plasma under the atmospheric condition was 310~380nm. Its energy is enough to excite the $TiO_2$ photocatalyst and it makes a photochemical reaction in the surface of $TiO_2$ photocatalyst. The removal quantity of trimethylamine$((CH_3)_3N)\; was\; 130mg/m^34 which is twice quantity of pulsed discharge plasma without $TiO_2$ phtocatalyst unit. From the result of gas analysis using FT-IR, nitric oxide was not detected and trimethylamine was decomposed to $H_2O\; and \;CO_2$. And trimethylamine removal efficiency was 95%. These experimental results indicate that photocatalytic plasma air cleaning unit is a potential method in removing the pollutants.

• Korean Journal of Food Science and Technology
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• v.41 no.5
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• pp.509-514
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• 2009

The effectiveness of a technique for binding rice-washed solution with trimethylamine (TMA) was investigated in this study. The mixtures of TMA and rice-washed solution were quantified using an electronic nose based on the mass spectrometer. After 7 min of reaction in a model system, the binding of TMA to the rice-washed solution was detected. As the concentration of rice-washed solution increased, the levels of TMA detected in the headspace decreased, thereby indicating an increase in the binding of TMA to the rice-washed solution. The binding effect of the rice-washed solution was comparable to those of starch, ${\alpha}$-cyclodextrin, or amylose, and superior to that of flour. The results of this study demonstrate the potential for reduction of off-flavors using the rice-washed solution.

• Journal of Korean Society for Atmospheric Environment
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• v.25 no.6
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• pp.512-522
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• 2009

In this study, the removal efficiency of trimethylamine (TMA) and four reduced sulfur compounds (RSC) of $H_2S$, $CH_3SH$, DMS, and DMDS was investigated using deionized water as absorbent. To this end, two types of experiments were conducted which include: (1) by passing both RSC and TMA standard gases through water contained in an impinger system and (2) by passing TMA gases through a water spray system. In the former method, TMA standard gases were effectively removed (below detection limit) in all experiments. Likewise, minor fractions of some RSC ($CH_3SH$, DMS, and DMDS) were removed by water in line with theoretical expectations. In addition, the first type of our experiment was extended further to test some commercially available odor treatment products. The results of this test showed that removal efficiencies of RSC $(8.2\pm13.7\sim43.7\pm2.48%)$ were different significantly among 4 kinds of absorbent, while the removal of TMA was consistently good as water. In the second phase of experiment, removal efficiency of TMA was tested by passing its standard gas through a water spray system. The overall results of our study showed that the actual removal efficiency of odorants by water (as sorptive media) approached the values that can be predicted theoretically (by Henry's law).

• Journal of Microbiology and Biotechnology
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• v.30 no.10
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• pp.1510-1515
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• 2020

Trimethylamine (TMA) is a well-known off-odor compound in fish and fishery products and is a metabolic product of trimethylamine N-oxide (TMAO) generated by the enzymatic action of microorganisms. The off-odor is a factor that can debase the value of fish and fishery products. The present study aimed to remove TMA using lactic acid bacteria (LAB). A total of fifteen isolates exhibiting the TMA reduction efficacy were isolated from Korean traditional fermented foods. Among these isolates, five LAB isolates (Lactobacillus plantarum SKD 1 and 4; Lactobacillus paraplantarum SKD 15; Pediococcus stilesii SKD 11; P. pentosaceus SKD 14) were selected based on their high TMA reduction efficacy. In situ reduction of TMA efficacy by the LAB cell-free supernatant was evaluated using a spoiled fish sample. The results showed effective TMA reduction by our selected strains: SKD1 (45%), SKD4 (62%), SKD11 (60%), SKD14 (59%), and SKD15 (52%), respectively. This is the first study on TMA reduction by the metabolic activity of LAB and in situ reduction of TMA using cell-free supernatant of LAB. The present finding suggests an economically useful and ecofriendly approach to the reduction of TMA.