• 제목/요약/키워드: trichome

검색결과 100건 처리시간 0.031초

Morphological Characteristics of Leaves, Trichomes and acorns in artificial hybrids of Quercus aliena ${\times}$ Q. mongolica var. crispula and Q. serrata ${\times}$ Q. mongolica var. crispula hybrids

  • Lee, Jeong-Ho;Kang, Kyu-Suk
    • 한국자원식물학회지
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    • 제22권3호
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    • pp.266-272
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    • 2009
  • Morphological characteristics of leaves, trichomes and acorns were investigated in 6-year-old artificial hybrids of Q. aliena ${\times}$ Q. mongolica var. crispula and Q. serrata ${\times}$ Q. mongolica var. crispula. Leaf shapes of Q. aliena ${\times}$ Q. mongolica var. crispula $F_{1}$ were obovate and resembled to that of Q. aliena. But several characters including the size of leaf and petiole and the shape of leaf base resembled to those of Q. mongolica var. crispula. In F1 hybrids, small stellate hairs distributed sparsely on the abaxial surface and their lay length was intermediate between both parents. There were no big differences on characters of nuts and cupules between both parents and $F_{1}$ hybrids. Leaf shapes of Q. serrata ${\times}$ Q. mongolica var. crispula $F_{1}$ were obovate-elliptic, and the leaf shape and leaf base and the length of petiole resembled to those of Q. mongolica var. crispula, but leaf size and serration resembled to those of Q. serrata. The number of serration in a leaf was intermediate between both parents. Small stellate hairs distributed sparsely and large single hairs were mixed on the reverse side of leaves. there were no big differences on the number and size of stellate hairs between $F_{1}$ hybrid and Q. serrata. It is able to distinguish $F_{1}$ hybrids from both parents by the size leaf size and shapes, leaf base and serration, petiole length and trichome type in the leaf.

한반도에 자생하는 돌뽕나무(Morus tiliaefolia Makino)의 형태학적 특성 (The Morphological Characteristics of Morus tiliaefolia Makino Growing Wild in the Korean Peninsula)

  • 박광준
    • 한국잠사곤충학회지
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    • 제44권1호
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    • pp.13-18
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    • 2002
  • 한반도에 자생하는 돌뽕나무를 수집하여 그 형태학적 특성을 조사한 결과는 다음과 같다 1.체세포의 염색체수는 2n=84인 6배체이다. 2.웅화수는 3.6~6.0cm의 긴 원통형이고 무화주인 자화수의 길이는 18 mm로 왜소하고 자 색 또는 자홍색의 오디는 1.39~l .65 g이다. 3. 동동아는 가지에 비하여 매우 크고 부아는 존재하지 않는다. 4. 잎은 대형의 열엽 또는 전연엽이며, 피목 밀도는 9.42$\pm$1.29개/$\textrm{cm}^2$이다. 5. 잎의 모용 길이는 236~438$\mu\textrm{m}$이고, 이면에 밀도가높은 바 16~37개/25 $\textrm{mm}^2$분포한다. 6. 잎의 표면의 모용형태는 원추형, 이면의 것은 매우 세장한 원추형이다

애기장대 (Arabidopsis thaliana) 잎 절편에서의 기관형성 특히 Naphthaleneacetic acid의 농도에 따른 부정근, 모용 및 캘러스 형성과 Determination Time (Organ Formation-The Formation of Adventitious Roots, Trichomes and Calli from Leaf Segments of Arabidopsis thaliana by Naphthaleneacetic acid Concentrations, and Their Determination times)

  • 한태진;김인현;김송림;김준철;임창진;진창덕
    • 식물조직배양학회지
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    • 제26권3호
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    • pp.211-217
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    • 1999
  • 애기장대 (Arabidopsis thaliana) 생태종 'Columbia' 잎절편에서의 묘조, 부정근, 모용 및 캘러스 형성에 미치는 auxin과 cytokinin의 효과를 조사하였다. 광범위한 식물생장 조절물질 농도와 조합에서 묘조, 부정근, 모용 및 캘러스가 형성되었다. 묘조는 0.1mg/L IAA와 10mg/L BA에서 형성되었고, 부정근은 저농도의 IAA와 NAA에서 모두 형성되었다. 모용과 캘러스는 IAA와 NAA의 농도 증가에 따라 형성되었으나 모용은 0.5mg/L NAA와 0.1mg/L BA 혼합처리구에서 가장 많이 형성되었으며, 캘러스는 10mg/L NAA와 10mg/L BA혼합처리구에서 가장 많이 형성되었다. NAA의 단독 영향을 조사한 결과 부정근 형성은 0.1mg/L 모용은 2.0mg/L 캘러스는 10mg/L가 가장 효과적이었으며, NAA배지에 각각 일정 기간 잎 절편을 치상하였다가 NAA 무처리 배지에 옮긴 결과 부정근, 모용 및 캘러스을 형성하는 determination time은 각각 6, 7, 18일이었다.

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Hybridization of Quercus aliena Blume and Q. serrata Murray in Korea - Analyses of Morphological variation and Flavonoid chemistry -

  • Park, Jin Hee;Park, Chong-Wook
    • 한국환경생태학회지
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    • 제29권2호
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    • pp.145-161
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    • 2015
  • This research was conducted in order to understand the hybridization between Quercus aliena Blume and Q. serrata Murray in Korea which show wide range of morphological variations within species and interspecific variations of diverse overlapping characteristics caused by hybridization. Morphological analysis (principal components analysis; PCA) of 116 individuals representing two species and their intermediates were performed. As a result, two species were clearly distinguished in terms of morphology, and intermediate morpho-types assumed to be hybrids between the two species were mostly located in the middle of each parent species in the plot of the principal components analysis. There was a clear distinction between two species in trichome distribution pattern which is an important diagnostic character in taxonomy of genus Quercus, whereas intermediate morpho-types showed intermediate state between two species' trichome distributions. Forty-two individuals representing two species and their intermediates were examined for leaf flavonoid constituents. Twenty-three flavonoid compounds were isolated and identified: They were glycosylated derivatives of flavonols, kaempferol, quercetin, isorhamnetin and myricetin. The flavonoid constituents of Q. aliena were five glycosylated derivatives: kaempferol 3-O-galactoside, kaempferol 3-O-glucoside, quercetin 3-O-galactoside, quercetin 3-O-glucoside, and Isorhamnetin 3-O-glucoside. The flavonoid constituents of Q. serrata had 20 diverse flavonol compounds including five flavonoid compounds found in Q. aliena. It was found that there is a clear difference in flavonoid constituents of Q. aliena and Q. serrata. Flavonoid chemistry is very useful in recognizing each species and putative hybrids. The flavonoid constituents of intermediates were a mixture of the two species' constituents and they generally showed similar characteristics to morpho-types. The hybrids between Q. aliena and Q. serrata showed morphologically and chemically diverse characteristics and it is assumed that there are frequent interspecific hybridization and introgression.

The Anticancer Role of Capsaicin in Experimentally-induced Lung Carcinogenesis

  • Anandakumar, Pandi;Kamaraj, Sattu;Jagan, Sundaram;Ramakrishnan, Gopalakrishnan;Asokkumar, Selvamani;Naveenkumar, Chandrashekar;Raghunandhakumar, Subramanian;Vanitha, Manickam Kalappan;Devaki, Thiruvengadam
    • 대한약침학회지
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    • 제18권2호
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    • pp.19-25
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    • 2015
  • Objectives: Capsaicin (CAP) is the chief pungent principle found in the hot red peppers and the chili peppers that have long been used as spices, food additives and drugs. This study investigated the anticancer potential of CAP through its ability to modify extracellular matrix components and proteases during mice lung carcinogenesis. Methods: Swiss albino mice were treated with benzo(a) pyrene (50 mg/kg body weight dissolved in olive oil) orally twice a week for four successive weeks to induce lung cancer at the end of $14^{th}$ week. CAP was administrated (10 mg/kg body weight dissolved in olive oil) intraperitoneally. Extracellular matrix components were assayed; Masson's trichome staining of lung tissues was performed. Western blot analyses of matrix metalloproteases 2 and 9 were also carried out. Results: In comparison with the control animals, animals in which benzo(a)pyrene had induced lung cancer showed significant increases in extracellular matrix components such as collagen (hydroxy proline), elastin, uronic acid and hexosamine and in glycosaminoglycans such as hyaluronate, chondroitin sulfate, keratan sulfate and dermatan sulfate. The above alterations in extracellular matrix components were effectively counteracted in benzo(a)pyrene along with CAP supplemented animals when compared to benzo(a) pyrene alone supplemented animals. The results of Masson's trichome staining for collagen and of, immunoblotting analyses of matrix metalloproteases 2 and 9 further supported the biochemical findings. Conclusion: The apparent potential of CAP in modulating extracellular matrix components and proteases suggests that CAP plays a chemomodulatory and anti-cancer role working against experimentally induced lung carcinogenesis.

한국산 팥꽃나무과 잎표피 미세구조의 분류학적 검토 (The taxonomic consideration of leaf epidermal microstructure in Korean Thymelaeaceae Adans)

  • 정은희;홍석표
    • 식물분류학회지
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    • 제33권4호
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    • pp.421-433
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    • 2003
  • 한국산 팥꽃나무과(Thymelaeaceae Adans.)의 5속(팥꽃나무속: 4종, 아마풀속: 1종, 삼지닥나무속: 1종, 피뿌리풀속: 1종, 산닥나무속: 2종)의 잎표피 미세구조를 광학현미경(LM)과 주사전자현미경(SEM)으로 관찰하였다. 연구된 모든 분류군은 이면기공엽(hypostomatic type)으로 밝혀졌다. 공변세포의 크기는 $13.8-34.4{\times}8.7-22.9{\mu}m$로 종에 따라 차이를 보이는데, Diathron linifolium($15.9{\pm}2.6{\times}10.0{\pm}1.3{\mu}m$)가 가장 작은 공변세포를 갖고 있었고, Daphne odora ($32.8{\pm}1.6{\times}20.7{\pm}1.3{\mu}m$)가 다소 큰 공변세포를 가지는 것으로 나타났다. 기공복합체의 형태는 대부분의 분류군에서 불규칙형(anomocytic)으로 나타났다. 다만, Daphne kiusiana의 경우는 불균등형(anisocytic)도 함께 나타났다. 부세포의 수층벽(anticlinal wall)은 직선형 또는 파상형이다. 표피세포의 크기는 $20.7-61.0{\mu}m$으로, 가장 작은 세포는 Stellera chamaejasme($26.0{\pm}1.9{\mu}m$)에서 나타났고, 가장 큰 세포는 Edgeworthia chrysantha($53.6{\pm}3.1{\mu}m$)에서 나타났다. 표피세포의 표변은 표피상납질(epicuticular wax)이 판 모양의 조각(flake-like platelet)들로 박혀있었으며, 표피침적정도에 따라 크게 세 가지 유형으로 구분되었다 - (1) 표피 상납질은 매끄러우며 완전하지 않은 판모양의 조각들이 존재함, (2) 납작한 판 모양의 조각들이 독립적으로 평행하게 산재함, (3) 막질의 판 모양 조각들이 표면으로부터 연결되어 다양한 각도로 조밀하게 산재함. 모용은 일부 분류군에서만 나타났으며, 엽연과 맥에 분포하는 분류군과 옆 전체에 분포하는 분류군으로 구분되었다. 모용이 있는 경우는 크게 두 가지 type으로 구별되었다. 즉, (1) Type I: 유선상이며, 선형단세포모(D. genkwa, E. chrysantha, Wikstroemia ganpi, W. trichotoma)와 (2) Type II: 유두상이며, 3-4개의 절로 구성된 갈고리형 다세포모(Diarthron linifoliuml로 나뉘었다. 끝으로 조사된 분류군내의 속간 그리고 종간의 동정과 식별을 위한 잎 표피 미세형태학적 형질의 분류학적 가치를 검토하였다.

나도국수나무족(장미과) 잎 표피 미세형태학적 형질의 계통학적 유용성 (The systematic implications of leaf micromorphological characteristics in the tribe Neillieae (Spiraeoideae, Rosaceae))

  • 송준호;홍석표
    • 식물분류학회지
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    • 제47권3호
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    • pp.222-235
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    • 2017
  • 나도국수나무족에 속하는 Neillia속(나도국수나무속) 4종 4변종, Physocarpus속(산국수나무속) 5종, Stephanandra속(국수나무속) 2종을 포함, 총 3속 15분류군의 잎 표피 미세형태학적 형질을 주사전자현미경(scanning electron microscope)을 이용하여 관찰 및 기재하였으며, 형질의 분류학적, 계통학적 유용성을 검토하였다. Neillia속, Stephanandra속에서 기공복합체는 모두 배축면에만 존재하는 이면기공엽(hypostomatic type)이 나타났고, Physocarpus속에서는 P. monogynus, P. opulifolius에서만 양면기공엽(amphistomatic type)이 나타났다. 공변세포의 크기는 $12.02-34.39{\times}10.76-27.13{\mu}m$로 속과 종마다 다소 차이를 보이는데, N. thyrsiflora가 평균 $13.98{\times}12.43(L{\times}W){\mu}m$로 가장 작은 공변세포를 갖는 것으로 나타났으며, N. gracilis가 평균 $26.82{\times}20.67(L{\times}W){\mu}m$로 가장 크게 나타났다. 기공복합체의 형태는 N. affinis에서만 평행형(paracytic)이 나타났고, 대부분 불규칙형(anomocytic)이 확인되었다. 표피세포는 섬국수나무(Physocarpus insularis)에서만 배축면에서 유두상(papillate)이 관찰되어 구별되었으며, N. affinis, S. tanakae의 향축면에서 판형(platelets)의 표피상납질(epicuticular wax)이 나타나 구별되었다. 한편, 연구된 분류군에서 모용은 크게 단세포단모(unicellular non-glandular trichome), 2-5개 가지상 모용(two- to five-armed trichome), 성상모(stellate), 선모(glandular trichome), 총 4종류로 확인되었다. 특히, 섬국수나무를 제외한 Physocarpus속 모든 분류군에서 성상모가 나타나 구별되었다. 끝으로, 잎 표피 미세형태학적 형질 중 기공복합체의 형태, 유두상표피세포의 유무, 성상모의 유무 형질이 분류학적 및 계통학적으로 유용성이 있음을 밝혔고, 본 연구 결과, Stephanandra속의 Neillia속으로의 통합, 섬국수나무(Physocarpus insularis)의 Spiraea속(조팝나무속)으로의 전속을 주장한 이전의 분자계통학적 및 화분학적 연구 결과를 강력하게 지지하였다.

Immunochemical Localization of Tetrahydrocannabinol (THC) in Chemically Fixed Glandular Thrichomes of Cannabis (Cannabaceae)

  • Eun Soo Kim;Paul G. Mahlberg
    • Animal cells and systems
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    • 제3권2호
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    • pp.215-219
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    • 1999
  • Monoclonal antibody for delta-9-tetrahydrocannabiol (THC Ab), conjugated with protein A-gold, was employed as a probe to detect THC localization in the gland and subjacent cells of chemically fixed bracts of Cannabis. THC was detected in the outer wall of the disc cells, fibrillar matrix, the surface feature of secretory vesicles, and sheath throughout development of the secretory cavity. The probe was absent from vesicles. Label was also present in anticlinal walls of disc cells and walls of dermal and mesophyll cells. Little or no THC Ab was present in disc cells and none were detected in control tissues. This distribution pattern of THC Ab was similar to that in tissues prepared by high pressure cryofixation-cryosubstitution. Consistent association of THC with wall and wall-derived materials suggests that cannnabinoids are synthesized outside the plasma membrane and bound to a wall component, where-upon they are transported to the cavity with wall materials released from the disc cell wall during development of the secretory cavity.

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Imidazole 염색에 의한 분비지질의 조직화학적 연구 (Histochemical Study of Secretory Lipids by Imidazole Staining)

  • 김은수
    • Applied Microscopy
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    • 제30권2호
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    • pp.113-119
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    • 2000
  • Lipophilic glandular trichomes form secretory vesicles that accumulate in a distended noncellular secretory cavity . Imidazole-buffered osmium tetroxide solution was used to visualize unsaturated lipids in glands of Cannabis sativa. This method of staining revealed two kinds of secretory vesicles in the cavity of glands. Some smaller and rounded vesicles in the secretory cavity and secretory cells were positively stained with the imidazole, and they appeared electron-dense. The other type of vesicles which have bigger sizes more or less were not reacted, however, they appeared transparent. A high contrast of the cuticles which cover the gland was also strongly reacted with that processing. Those result suggest that the dark vesicles in the cavity may contribute to enlarge the subcuticular wall and cuticle when contents of these vesicles are dispersed into wall.

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고추 모용(毛茸)의 유전(遺傳) (Inheritance of Stem Pubescence in Red Pepper)

  • 박규환;신성련
    • Current Research on Agriculture and Life Sciences
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    • 제6권
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    • pp.71-73
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    • 1988
  • 고추 줄기의 모용(毛茸)에 대(對)한 유전현상(遺傳現象)을 구명(究明)하기 위하여 342, 김장고추, 남지(南旨), Hungarian Wax등(等) 4품종(品種)과 이들을 교잡(交雜)하여 얻은 $F_1$, $F_2$$BC_1F_1$에 대(對)해 시험한 결과(結果)를 요약(要約)하면 고추 줄기의 모용(毛茸)이 많은 것이 없는 것에 대(對)하여 우성(優性)이었으며 이 형질(形質)은 단일유전인자(單一遺傳因子)에 의(依)하여 지배(支配)되는 것으로 나타났다.

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