• Korean Journal of Plant Taxonomy
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• v.34 no.4
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• pp.273-285
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• 2004

A comparative micromorphology of petal and sepal of 15 species of Lycopus was undertaken to assess their usefulness in species identification and to evaluate their significance in the taxonomy using scanning electron microscope and stereo microscope. Five types of trichome are found within the genus: unicellular cylindrical trichome, unicellular trichome with papillae, simple multicellular trichome, capitate glandular trichome, and peltate glandular trichome. The types, distribution, and density of the trichomes show considerable variation among the taxa. The distributional pattern of the unicellular cylindrical trichomes on inner side of corolla is differed among the investigated taxa, and can be classified into four patterns. The apex shape of sepal and the incised position of calyx are also various and be recognizedas four patterns. The shape of cell composing in both outer and inner sides of petal and sepal are shown variously. Finally, the taxonomic significance of micromorphology of the petal and sepal in identification and elucidation of the genus Lycopus, especially among the species is also briefly discussed.

• Korean Journal of Plant Resources
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• v.13 no.3
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• pp.179-187
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• 2000

The achene morphology about 16 taxa of Korean Aster L. sensu lato were investigated to estimate its taxonomic values. The achene shapes were divided into four types; oblanceolate-oblong, obovate, oblong and obovate-oblong. The trichome shape on achene six types; uniseriate-conical, filiform, cylindrical, capitate type, long stalk capitate and globular. Their distributional features on upper part of achene four types; absent, sparse distribution of conical trichome, dense distribution of conical trichome and mixed distribution of conical and capitate trichome. The achene shapes and trichome characteristics were regarded to be a good characters in delimiting taxa because these did not differ among individuals in same taxa, but differ among the taxa. If Korean Aster L. sensu late were divided into Kalimeris, Heteropappus, Aster, Cymnaster, the capitate forms and mixed distribution of conical and capitate trichome were recognized as the good characters in delimting above section such as genus Kalimeris and Heteropappus, section Pseudocalimeris of Aster L. sensu stricto.

• Journal of Plant Biology
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• v.38 no.3
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• pp.289-295
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• 1995

Trichomes found in the genus Cimicifuga were examined with scanning electron microscopy. Four basic types of trichomes were found in the genus; (1) filiform unicellular trichomes, (2) saccate unicellular trichomes, (3) pyriform unicellular trichomes, and (4) uniseriate multicellular trichomes. All the taxa examined develop at least two different types of trichomes, and many taxa are distinguished by differences in type, microornamentation, density, and position of trichomes. In addition, trichome features appear to be very useful in recognizing species relationships in the genus.

• Applied Microscopy
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• v.28 no.2
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• pp.159-170
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• 1998

The glandular secretory system of the capitate gandular trichomes in leaf of Thymus quinquecostatus Celakovsky was examined by transmission electron microscope. The glandular trichome was consisted of three cell layers; an basal cell layer, a stalk cell with single-celled intermediate layer and a discoid secretory layer with thickened cuticle. The secretory cell was dense, rich in mitochondria, rER, plastds, Golgi complex and had many vesicular structure. Typical plastids with reticulate body and plastoglobule were present in glandular trichome. The tytoplasm of secretory cell was filled with osmiophilic secretory materials. The secretory vesicles, originated from Golgi complex, appeared as membrane bounded vesicles and secreted to the outer wall surface. The presences of well developed rER, mitochondria, Golgi complex, and membrane-bounded vesicles fused with plasmalemma in the secreting cells indicate that the granulocrine mechanism of secretion was occurring in T. quinquecostatus. Subcuticular cavity was developed between the cuticular layer and the secretory cell wall, and it formed above the secretory cell upon separation of cuticle-wall.

• Applied Microscopy
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• v.32 no.4
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• pp.319-327
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• 2002

Salvinia natans, an unique water fern having a small rootless body, developed three different types of trichomes throughout the plant. The most peculiar type exhibiting rows of obvious, whitish, multicellular trichome clusters was noticed in the upper surface of the floating leaves. Eight to ten branches within a cluster extended ca. $370{\sim}420{\mu}m$ from the leaf surface. No stalk cell was found, however, four large epidermal cells were discernable at the base of four central branches in the cluster. Each branch consisted of $8{\sim}10$ obliquely-oriented small cells that gradually decreased in size toward the branch tip. The second type was found in the lower surface of the floating leaves, stems, and sporocarps. Multicellular uniseriate trichomes, ca. $430{\sim}980{\mu}m$ long, were distributed all over these structures. The tip of trichome was acicular, but a semi-spheric protuberance of approximately $24{\sim}32{\mu}m$ in diameter occurred at the base of each trichome. The protuberance appeared to be firmly attached to the side of the basal cell, however, internal connection to the trichome cell itself was uncertain. The third type was similar to the second in that multicellur uniseriate trichomes with acicular tip and a protuberance at the base were present. However, the trichomes were considerably long relative to the second type, and only occurred along the surface of highly dissected, submerged leaves. A majority of the trichomes exceeded more than 2 mm in length that hung downward in the water. Regardless of trichome type, all trichomes contained a huge central vacuole with very thin cytoplasm, resulting from the fusion of several vacuoles during early trichome development. The various densely-distributed trichomes formed in Salvinia natans probably play an important role in plant buoyancy.

• The Plant Pathology Journal
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• v.28 no.1
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• pp.107-113
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• 2012

Trichomes are specialized epidermal structure having the functions of physical and chemical block against biotic and abiotic stresses. Several studies on $Capsicum$ species revealed that virus and herbivore resistance is associated with trichome-formation. However, there is no research on the structural characterization of trichomes developed on the epidermis of $Capsicum$ spp. Thus, this study attempts to charaterize the trichome morphologies in 5 species of $Capsicum$ using a Field Emission Scanning Electron Microscopy (FESEM). Six main trichome types were identified by their morphology under FESEM. Both glandular and non-glandular types of trichomes were developed on the epidermal tissues of $Capsicum$ spp. The glandular trichome were further classified into type I, IV and VII according to their base, stalk length, and stalk. Non-glandular trichomes were also classified into type II, III, and V based on stalk cell number and norphology. Almost all the species in $C.$ $chinense$ and $C.$ $pubescens$ had glandular trichomes. To our knowledge, this is the first study on classification of trichomes in the genus $Capsicum$ and, our results could provide basic informations for understanding the structure and function of trichomes on the epidermal differentiation and association with biotic stress tolerance.

• Korean Journal of Plant Taxonomy
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• v.48 no.3
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• pp.185-194
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• 2018

A comparative floral morphological study of 19 taxa in Persicaria sect. Cephalophilon with four taxa related to Koenigia was conducted to evaluate the taxonomic implications. The flowers of P. sect. Cephalophilon have (four-)five-lobed tepals; five, six, or eight stamens, and one pistil with two or three styles. The size range of each floral characteristic varies according to the taxa; generally P. humilis, P. glacialis var. glacialis and Koenigia taxa have rather small floral sizes. The connate degrees of the tepal lobes and styles also vary. The tepal epidermis consists of elongated rectangular cells with variation of the anticlinal cell walls (ACWs). Two types of glandular trichomes are found. The peltate glandular trichome (PT) was observed in nearly all of the studied taxa. The PT was consistently distributed on the outer tepal of P. sect. Cephalophilon, while Koenigia taxa and P. glacialis var. glacialis had this type of trichome on both sides of the tepal. P. criopolitana had only long-stalked pilate-glandular trichomes (LT) on the outer tepal. The nectary is distributed on the basal part of the inner tepal, with three possible shapes: dome-like, elongated, and disc-like nectary. The nectaries are always accompanied by elongated or spheroidal papillae. Various combinations of floral characters (e.g., the numbers of stamens and styles, the stigma shape, the nectary shape, ACWs, cuticular striation and the trichome type and distribution) of P. sect. Cephalophilon are useful when attempting to recognize the infrasectional levels of P. sect. Cephalophilon recently proposed. Here, we describe the floral characteristics in detail and discuss the taxonomic significance of the floral characters.

• Korean Journal of Plant Taxonomy
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• v.46 no.2
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• pp.199-212
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• 2016

A comparative study of leaf epidermal microstructures in the tribe Sorbarieae (Adenostoma: 3 spp., Chamaebatiaria: 1 sp., Sorbaria: 11 spp., Spiraeanthus: 1 sp.) including related genera Gillenia (2 spp.) and Lyonothamnus (2 spp.) was carried out using scanning electron microscopy (SEM) in order to evaluate their significance in taxonomy. The leaves of Adenostoma, Chamaebatiaria, and Spiraeanthus were amphistomatic, whereas Gillenia, Lyonothamnus, and Sorbaria were hypostomatic. The size range of the guard cells is $7.84-48.7{\times}5.86-38.6{\mu}m$; the smallest one was found in Sorbaria tomentosa var. tomentosa ($7.84-11.8{\times}6.84-10.5{\mu}m$), while the largest measured example was Adenostoma fasciculatum var. obtusifolium ($30.3-48.7{\times}18.8-38.6{\mu}m$). Anomocytic stomata complex were the most frequent type (rarely cyclocytic), with usually both anomocytic and actinocytic types occurring in one leaf. On the surfaces, both the adaxial and abaxial anticlinal walls of the subsidiary cells vary (e.g., straight/curved, undulate, sinuate). Four types (unicellular non-glandular trichome, stellate, glandular trichome, pustular glandular trichome) of trichomes are found in the leaves. The epicuticular wax can be divided two types: membraneous platelets (Lyonothamnus) and platelets (Sorbaria arborea var. arborea, S. arborea var. subtomentosa, S. kirilowii, S. tomentosa var. tomentosa, Spiraeanthus schrenkianus). The trichome diversity (in particular, stellate, gland) and the existence of epicuticular wax may have taxonomic significance, although the leaf epidermal micromorphological characteristics do not provide synapomorphy in this tribe. These leaf micromorphological features are most likely better understood in the Sorbarieae when used in conjunction with external morphological characters.

• Applied Microscopy
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• v.43 no.1
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• pp.34-39
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• 2013

Glandular and nonglandular trichomes on the leaf surface of Plectranthus tomentosa were investigated by variable pressure field emission scanning electron microscopy (VP-FESEM). The segments of the plant's leaves were directly mounted without any specimen preparation, and examined at ambient temperature using a variable pressure secondary electron (SE) detector under ca. 15 Pa. Foliar trichomes maintained their shapes and structures without severe surface collapse or charging. The adaxial leaf surface was abundantly covered with different types of trichome. Nonglandular trichomes consisted of a basal cell and a long (up to ca. $300{\mu}m$) stalk. Meanwhile, capitate glandular trichomes had a secretory head and a short or long stalk. Peltate glandular trichomes with globose secretory heads were observed in close contact with the leaf epidermis. Spherical projections on the secretory head showed the secretion process of glandular trichomes. In addition to the trichomes, oval stomata were distributed on the abaxial leaf surface. These results suggest that ambient VP-FESEM can be used to classify the dehydration-sensitive foliar trichomes of succulent plants by SE imaging. At the FESEM resolution, this approach facilitates the rapid and detailed morphological analysis of a variety of trichomes in diverse plant taxa with reduced labor and preparation.

• Korean Journal of Plant Taxonomy
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• v.44 no.1
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• pp.22-32
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• 2014

The petal and sepal micromorphology of five species of Glechoma (Lamiaceae) was investigated to evaluate their taxonomic significance, and a molecular phylogeny using the sequences of internal transcribed spacers (ITS) regions of nuclear ribosomal DNA was carried out to resolve their phylogenetic relationships. Stomatal complexes were mostly found in the inner and outer part of the sepal from all investigated taxa, and the size length of the guard cell was variable among the taxa. Five types of trichomes (uni-cellular non-glandular trichome, multi-cellular non-glandular trichome, short-stalked capitate glandular trichome, long-stalked capitate glandular trichome, and peltate glandular trichome) were variable among the taxa as well as their distribution and density. In molecular phylogenetic studies, the genus Glechoma was composed of three geographically distinct major monophyletic groups (Europe-U.S.A., China-Korea, Japan). G. longituba in Korea and China formed well-supported monophyletic group. G. hederacea in Europe and U.S.A. formed a monophyletic and well-supported clade with G. sardoa, which are endemic species in Italy, with G. hirsuta falling as a sister to this clade. However, G. grandis did not form any phylogenetic relationships with the remaining taxa. The ITS analyses provided taxonomic boundaries of taxa in Glechoma although the petal and sepal micromorphological characters provided weak evidences of the systematic value. As further studies, incorporating more DNA regions to the matrix including other additional morphological analysis will be significant to provide clearer taxonomic structure in Glechoma.